Restriction Fragments for CUP1-1/YHR053C



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The currently selected gene/sequence is :

CUP1-1/YHR053C on chromosome VIII from coordinates 212720 to 212535.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.12683.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 11 14 + TspEI AATT 10 14 . . 12 17 + VspI ATTAAT 13 15 . . 13 20 + PacI TTAATTAA 17 15 . . 13 16 + MseI TTAA 13 15 . . 15 18 + TspEI AATT 14 18 . . 17 20 + MseI TTAA 17 19 . . 29 33 + TspDTI ATGAA 44 42 . . 33 36 + SetI ASST 36 32 . . 31 35 - Hin4II* CCTTC 25 24 . . 36 41 + Hpy178III* TCNNGA 37 39 . . 36 41 + BspHI TCATGA 36 40 . . 37 40 + FatI CATG 36 40 . . 37 40 + NlaIII CATG 40 36 . . 37 40 + CviAII CATG 37 39 . . 45 59 + XcmI CCANNNNNNNNNTGG 52 51 . . 61 64 + SetI ASST 64 60 . . 61 64 + CviJI RGCY 62 62 . . 61 64 + AluI AGCT 62 62 . . 62 66 + BlsI GCNGC 64 63 . . 62 66 + BisI GCNGC 63 64 . . 62 66 + TseI GCWGC 62 65 . . 64 67 + CviRI* TGCA 65 65 . . 62 66 - BbvI GCAGC 49 53 . . 74 78 + TspDTI ATGAA 89 87 . . 92 95 + FatI CATG 91 95 . . 92 95 + NlaIII CATG 95 91 . . 92 95 + CviAII CATG 92 94 . . 97 100 + SetI ASST 100 96 . . 97 100 + CviJI RGCY 98 98 . . 97 100 + AluI AGCT 98 98 . . 98 102 + BlsI GCNGC 100 99 . . 98 102 + BisI GCNGC 99 100 . . 98 102 + TseI GCWGC 98 101 . . 98 102 - BbvI GCAGC 85 89 . . 113 117 + MaeIII GTNAC 112 117 . . 120 124 + Hpy99I CGWCG 124 119 . . 140 144 + MaeIII GTNAC 139 144 . . 137 140 - AciI CCGC 137 139 . . 148 152 + Hpy188I TCNGA 150 149 . . 149 154 + Eco57MI CTGRAG 170 168 . . 149 154 + Eco57I CTGAAG 170 168 . . 151 155 + MboII GAAGA 163 162 . . 167 170 + FatI CATG 166 170 . . 167 170 + NlaIII CATG 170 166 . . 167 170 + CviAII CATG 167 169 . . 170 174 + BlsI GCNGC 172 171 . . 170 174 + BisI GCNGC 171 172 . . 170 174 + TseI GCWGC 170 173 . . 170 174 - BbvI GCAGC 157 161 . . #--------------------------------------- # # # AciI: # [CCGC] # 49 137 # # AluI: # [AGCT] # 36 62 88 # # BbvI: # [GCAGC] # 29 36 49 72 # # BisI: # [GCNGC] # 15 36 63 72 # # BlsI: # [GCNGC] # 14 36 64 72 # # BspHI: # [TCATGA] # 36 150 # # CviAII: # [CATG] # 19 37 55 75 # # CviJI: # [RGCY] # 36 62 88 # # CviRI*: # [TGCA] # 65 121 # # Eco57I: # [CTGAAG] # 16 170 # # Eco57MI: # [CTGRAG] # 16 170 # # FatI: # [CATG] # 20 36 55 75 # # Hin4II*: # [CCTTC] # 25 161 # # Hpy178III*: # [TCNNGA] # 37 149 # # Hpy188I: # [TCNGA] # 36 150 # # Hpy99I: # [CGWCG] # 62 124 # # MaeIII: # [GTNAC] # 27 47 112 # # MboII: # [GAAGA] # 23 163 # # MseI: # [TTAA] # 4 13 169 # # NlaIII: # [CATG] # 16 40 55 75 # # PacI: # [TTAATTAA] # 17 169 # # SetI: # [ASST] # 28 36 36 86 # # TseI: # [GCWGC] # 16 36 62 72 # # TspDTI: # [ATGAA] # 44 45 97 # # TspEI: # [AATT] # 4 10 172 # # VspI: # [ATTAAT] # 13 173 # # XcmI: # [CCANNNNNNNNNTGG] # 52 134 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 186 # Reported_sequences: 1 # Reported_hitcount: 49 #---------------------------------------