Restriction Fragments for MCM6/YGL201C



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The currently selected gene/sequence is :

MCM6/YGL201C on chromosome VII from coordinates 120907 to 117854.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.4960.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 9 - BseGI GGATG 4 2 . . 17 22 + PvuII CAGCTG 19 19 . . 17 22 + NspBII* CMGCKG 19 19 . . 18 21 + SetI ASST 21 17 . . 18 21 + CviJI RGCY 19 19 . . 18 21 + AluI AGCT 19 19 . . 42 45 + SetI ASST 45 41 . . 43 47 + Hin4II* CCTTC 53 52 . . 46 51 + MmeI TCCRAC 71 69 . . 35 47 - BarI GAAGNNNNNNTAC 59 54 27 22 53 56 + MnlI CCTC 63 62 . . 51 56 - BseRI GAGGAG 42 40 . . 58 61 + AciI CCGC 58 60 . . 57 62 - EciI GGCGGA 47 45 . . 64 68 + BccI CCATC 72 73 . . 68 72 + Hpy99I CGWCG 72 67 . . 70 73 + TaqI TCGA 70 72 . . 75 78 + SetI ASST 78 74 . . 79 84 + Cac8I GCNNGC 81 81 . . 82 85 + CviJI RGCY 83 83 . . 83 93 + MwoI GCNNNNNNNGC 89 86 . . 86 90 + Hpy188I TCNGA 88 87 . . 101 105 + HinfI GANTC 101 104 . . 98 101 - AciI CCGC 98 100 . . 104 107 + TaqI TCGA 104 106 . . 101 105 - PleI GAGTC 95 96 . . 101 105 - MlyI GAGTC 95 95 . . 106 110 + TfiI GAWTC 106 109 . . 106 110 + HinfI GANTC 106 109 . . 118 121 + CviJI RGCY 119 119 . . 122 125 + MaeI CTAG 122 124 . . 124 127 + SetI ASST 127 123 . . 126 130 + MaeIII GTNAC 125 130 . . 142 146 - MboII GAAGA 134 133 . . 151 154 + AciI CCGC 151 153 . . 153 158 + NspI RCATGY 157 153 . . 154 157 + FatI CATG 153 157 . . 154 157 + NlaIII CATG 157 153 . . 154 157 + CviAII CATG 154 156 . . 163 166 + TspEI AATT 162 166 . . 174 180 + DraII RGGNCCY 175 178 . . 175 178 + CviJI RGCY 176 176 . . 175 178 + HaeIII GGCC 176 176 . . 175 179 + BmgT120I GGNCC 176 177 . . 175 179 + AsuI* GGNCC 175 178 . . 184 189 + Hpy166II GTNNAC 186 186 . . 184 189 + HpaI GTTAAC 186 186 . . 184 189 + HindII GTYRAC 186 186 . . 185 188 + MseI TTAA 185 187 . . 190 194 + TfiI GAWTC 190 193 . . 190 194 + HinfI GANTC 190 193 . . 197 201 + DdeI CTNAG 197 200 . . 197 201 + BspCNI CTCAG 210 208 . . 197 201 + BseMII CTCAG 211 209 . . 214 217 + SetI ASST 217 213 . . 218 221 + CviRI* TGCA 219 219 . . 229 234 + NlaIV GGNNCC 231 231 . . 226 230 - BccI CCATC 220 221 . . 233 236 + AciI CCGC 233 235 . . 234 237 + FnuDII* CGCG 235 235 . . 250 253 - MnlI CCTC 243 242 . . 257 261 + TspDTI ATGAA 272 270 . . 261 264 + SetI ASST 264 260 . . 261 266 + BspMI ACCTGC 270 274 . . 270 275 + HindIII AAGCTT 270 274 . . 271 274 + SetI ASST 274 270 . . 271 274 + CviJI RGCY 272 272 . . 271 274 + AluI AGCT 272 272 . . 279 289 + FalI AAGNNNNNCTT 270 265 302 297 285 288 + SetI ASST 288 284 . . 285 288 + CviJI RGCY 286 286 . . 285 288 + AluI AGCT 286 286 . . 279 289 - FalI AAGNNNNNCTT 302 297 270 265 291 295 + TfiI GAWTC 291 294 . . 291 295 + HinfI GANTC 291 294 . . 296 299 + TaiI ACGT 299 295 . . 296 299 + SetI ASST 299 295 . . 296 299 + MaeII ACGT 296 298 . . 299 302 + MseI TTAA 299 301 . . 314 317 + TaiI ACGT 317 313 . . 314 317 + SetI ASST 317 313 . . 314 317 + MaeII ACGT 314 316 . . 316 320 + MaeIII GTNAC 315 320 . . 319 323 + BsrI ACTGG 324 322 . . 322 326 + HphI GGTGA 334 333 . . 332 336 - TspGWI ACGGA 322 320 . . 344 347 + TaqI TCGA 344 346 . . 358 362 + MboII GAAGA 370 369 . . 358 363 + BbvII* GAAGAC 365 369 . . 367 371 - TspGWI ACGGA 357 355 . . 378 384 + TspRI CASTGNN 384 375 . . 385 389 + BsrI ACTGG 390 388 . . 388 392 + HphI GGTGA 400 399 . . 410 415 + SacI GAGCTC 414 410 . . 410 415 + Ecl136II GAGCTC 412 412 . . 410 415 + SduI GDGCHC 414 410 . . 410 415 + HgiJII* GRGCYC 414 410 . . 410 415 + HgiAI* GWGCWC 414 410 . . 411 414 + SetI ASST 414 410 . . 411 414 + CviJI RGCY 412 412 . . 411 414 + AluI AGCT 412 412 . . 417 420 + TspEI AATT 416 420 . . 421 426 + ApoI RAATTY 421 425 . . 422 425 + TspEI AATT 421 425 . . 427 431 + TspDTI ATGAA 442 440 . . 440 443 + SetI ASST 443 439 . . 477 480 + FatI CATG 476 480 . . 477 480 + NlaIII CATG 480 476 . . 477 480 + CviAII CATG 477 479 . . 491 496 + SduI GDGCHC 495 491 . . 491 496 + HgiAI* GWGCWC 495 491 . . 496 499 + MaeI CTAG 496 498 . . 498 501 + SetI ASST 501 497 . . 498 501 + CviJI RGCY 499 499 . . 498 501 + AluI AGCT 499 499 . . 504 507 + CviJI RGCY 505 505 . . 503 508 - TsoI TARCCA 493 491 . . 510 514 + DdeI CTNAG 510 513 . . 510 514 + BspCNI CTCAG 523 521 . . 510 514 + BseMII CTCAG 524 522 . . 511 515 + Hpy188I TCNGA 513 512 . . 518 523 + SspI AATATT 520 520 . . 553 556 + MseI TTAA 553 555 . . 557 561 + MboII GAAGA 569 568 . . 557 562 - Ksp632I* CTCTTC 552 555 . . 576 579 + CviRI* TGCA 577 577 . . 579 582 + SetI ASST 582 578 . . 584 587 + TspEI AATT 583 587 . . 589 592 + MseI TTAA 589 591 . . 599 603 + Tsp45I GTSAC 598 603 . . 599 603 + MaeIII GTNAC 598 603 . . 601 605 + HinfI GANTC 601 604 . . 601 605 - PleI GAGTC 595 596 . . 601 605 - MlyI GAGTC 595 595 . . 609 613 + MboII GAAGA 621 620 . . 613 618 + XhoII RGATCY 613 617 . . 614 617 + DpnI GATC 615 615 . . 614 617 + BstKTI GATC 616 614 . . 609 614 - Ksp632I* CTCTTC 604 607 . . 614 617 + MboI GATC 613 617 . . 616 620 + Hpy188I TCNGA 618 617 . . 614 618 - BinI* GGATC 608 609 . . 621 624 + SetI ASST 624 620 . . 622 626 + HphI GGTGA 634 633 . . 623 627 + Tsp45I GTSAC 622 627 . . 623 627 + MaeIII GTNAC 622 627 . . 619 623 - Hin4II* CCTTC 613 612 . . 624 635 + BdaI TGANNNNNNTCA 613 611 647 645 625 634 + PshAI GACNNNNGTC 629 629 . . 630 633 + SetI ASST 633 629 . . 628 632 - Hin4II* CCTTC 622 621 . . 624 635 - BdaI TGANNNNNNTCA 647 645 613 611 639 643 + FokI GGATG 652 656 . . 639 643 + BseGI GGATG 645 643 . . 637 640 - AciI CCGC 637 639 . . 641 645 + TspDTI ATGAA 656 654 . . 643 647 + MboII GAAGA 655 654 . . 667 671 + TspDTI ATGAA 682 680 . . 676 679 + TaqI TCGA 676 678 . . 678 682 + PleI GAGTC 686 687 . . 678 682 + MlyI GAGTC 687 687 . . 678 682 + HinfI GANTC 678 681 . . 680 685 + Hpy166II GTNNAC 682 682 . . 680 685 + AccI GTMKAC 681 683 . . 691 695 + TfiI GAWTC 691 694 . . 691 695 + HinfI GANTC 691 694 . . 691 701 + Hin4I GAYNNNNNVTC 682 677 714 709 694 699 + Hpy178III* TCNNGA 695 697 . . 697 701 + BinI* GGATC 705 706 . . 698 701 + DpnI GATC 699 699 . . 698 701 + BstKTI GATC 700 698 . . 698 701 + MboI GATC 697 701 . . 691 701 - Hin4I GAYNNNNNVTC 714 709 682 677 705 708 + CviJI RGCY 706 706 . . 706 710 + TauI GCSGC 709 706 . . 706 710 + BlsI GCNGC 708 707 . . 706 710 + BisI GCNGC 707 708 . . 707 717 + BsiYI* CCNNNNNNNGG 713 710 . . 707 710 + AciI CCGC 707 709 . . 712 716 + BssKI CCNGG 711 716 . . 712 716 + BseBI CCWGG 713 714 . . 712 717 + SecI* CCNNGG 712 716 . . 712 716 + ScrFI CCNGG 713 714 . . 712 716 + EcoRII CCWGG 711 716 . . 712 722 + BsiYI* CCNNNNNNNGG 718 715 . . 712 722 + PflMI CCANNNNNTGG 718 715 . . 721 725 + BslFI GGGAC 735 739 . . 731 736 + DsaI* CCRYGG 731 735 . . 731 736 + StyI CCWWGG 731 735 . . 731 736 + NcoI CCATGG 731 735 . . 731 736 + SecI* CCNNGG 731 735 . . 732 735 + FatI CATG 731 735 . . 732 735 + NlaIII CATG 735 731 . . 732 735 + CviAII CATG 732 734 . . 742 745 + SetI ASST 745 741 . . 741 744 - MnlI CCTC 734 733 . . 760 763 + TaiI ACGT 763 759 . . 760 763 + SetI ASST 763 759 . . 760 763 + MaeII ACGT 760 762 . . 762 766 + Tsp45I GTSAC 761 766 . . 762 766 + MaeIII GTNAC 761 766 . . 765 768 + SetI ASST 768 764 . . 763 767 - HphI GGTGA 755 754 . . 775 779 + TspGWI ACGGA 790 788 . . 780 783 + TaiI ACGT 783 779 . . 780 783 + SetI ASST 783 779 . . 780 783 + MaeII ACGT 780 782 . . 780 785 + AflIII ACRYGT 780 784 . . 800 804 - MboII GAAGA 792 791 . . 814 818 + Tsp4CI* ACNGT 816 815 . . 817 822 + Hpy166II GTNNAC 819 819 . . 824 829 + EcoRI GAATTC 824 828 . . 824 829 + ApoI RAATTY 824 828 . . 825 828 + TspEI AATT 824 828 . . 828 833 + Hpy178III* TCNNGA 829 831 . . 832 842 + Hin4I GAYNNNNNVTC 823 818 855 850 838 843 + XhoII RGATCY 838 842 . . 838 843 + BglII AGATCT 838 842 . . 839 842 + DpnI GATC 840 840 . . 839 842 + BstKTI GATC 841 839 . . 839 842 + MboI GATC 838 842 . . 841 845 + Hpy188I TCNGA 843 842 . . 832 842 - Hin4I GAYNNNNNVTC 855 850 823 818 854 859 + Hpy166II GTNNAC 856 856 . . 874 878 + BslFI GGGAC 888 892 . . 877 881 + Tsp4CI* ACNGT 879 878 . . 878 884 + TspRI CASTGNN 884 875 . . 880 884 + Tsp45I GTSAC 879 884 . . 880 884 + MaeIII GTNAC 879 884 . . 892 896 + Hpy188I TCNGA 894 893 . . 893 897 + DdeI CTNAG 893 896 . . 896 899 + SetI ASST 899 895 . . 893 897 - BspCNI CTCAG 885 883 . . 893 897 - BseMII CTCAG 884 882 . . 897 901 + BmgT120I GGNCC 898 899 . . 897 901 + AvaII GGWCC 897 900 . . 897 901 + AsuI* GGNCC 897 900 . . 895 898 - MnlI CCTC 888 887 . . 899 903 - TspGWI ACGGA 889 887 . . 903 908 + Hpy178III* TCNNGA 904 906 . . 908 911 + TspEI AATT 907 911 . . 916 926 + FalI AAGNNNNNCTT 907 902 939 934 919 924 + Cac8I GCNNGC 921 921 . . 921 926 + HindIII AAGCTT 921 925 . . 922 925 + SetI ASST 925 921 . . 922 925 + CviJI RGCY 923 923 . . 922 925 + AluI AGCT 923 923 . . 916 926 - FalI AAGNNNNNCTT 939 934 907 902 927 932 + BsaAI YACGTR 929 929 . . 928 931 + TaiI ACGT 931 927 . . 928 931 + SetI ASST 931 927 . . 928 931 + MaeII ACGT 928 930 . . 928 933 + AflIII ACRYGT 928 932 . . 941 945 - BceAI ACGGC 926 928 . . 959 962 + TaiI ACGT 962 958 . . 959 962 + SetI ASST 962 958 . . 959 962 + MaeII ACGT 959 961 . . 966 970 + Tsp4CI* ACNGT 968 967 . . 971 975 + Hin4II* CCTTC 981 980 . . 977 982 + TatI WGTACW 977 981 . . 978 983 + Hpy166II GTNNAC 980 980 . . 978 981 + Csp6I GTAC 978 980 . . 978 981 + RsaI GTAC 979 979 . . 981 987 + TspRI CASTGNN 987 978 . . 1006 1010 + BccI CCATC 1014 1015 . . 1010 1013 + FatI CATG 1009 1013 . . 1010 1013 + NlaIII CATG 1013 1009 . . 1010 1013 + CviAII CATG 1010 1012 . . 1023 1026 + CviJI RGCY 1024 1024 . . 1036 1039 + MseI TTAA 1036 1038 . . 1042 1046 + MaeIII GTNAC 1041 1046 . . 1048 1053 + XhoII RGATCY 1048 1052 . . 1049 1052 + DpnI GATC 1050 1050 . . 1049 1052 + BstKTI GATC 1051 1049 . . 1049 1052 + MboI GATC 1048 1052 . . 1052 1056 + BssKI CCNGG 1051 1056 . . 1052 1056 + BseBI CCWGG 1053 1054 . . 1052 1056 + ScrFI CCNGG 1053 1054 . . 1052 1056 + EcoRII CCWGG 1051 1056 . . 1049 1053 - BinI* GGATC 1043 1044 . . 1054 1057 + SetI ASST 1057 1053 . . 1059 1064 + Hpy178III* TCNNGA 1060 1062 . . 1076 1080 + Hpy188I TCNGA 1078 1077 . . 1079 1084 + EcoRI GAATTC 1079 1083 . . 1079 1084 + ApoI RAATTY 1079 1083 . . 1080 1083 + TspEI AATT 1079 1083 . . 1083 1088 + Hpy178III* TCNNGA 1084 1086 . . 1104 1107 + SetI ASST 1107 1103 . . 1106 1111 + SfeI* CTRYAG 1106 1110 . . 1111 1114 + CviJI RGCY 1112 1112 . . 1111 1116 + NlaIV GGNNCC 1113 1113 . . 1116 1119 + FatI CATG 1115 1119 . . 1116 1119 + NlaIII CATG 1119 1115 . . 1116 1119 + CviAII CATG 1116 1118 . . 1119 1123 + TauI GCSGC 1122 1119 . . 1119 1129 + MwoI GCNNNNNNNGC 1125 1122 . . 1119 1123 + BlsI GCNGC 1121 1120 . . 1119 1123 + BisI GCNGC 1120 1121 . . 1120 1123 + AciI CCGC 1120 1122 . . 1121 1124 + FnuDII* CGCG 1122 1122 . . 1123 1128 + SplI* CGTACG 1123 1127 . . 1124 1127 + Csp6I GTAC 1124 1126 . . 1124 1127 + RsaI GTAC 1125 1125 . . 1132 1137 + ZraI GACGTC 1134 1134 . . 1132 1137 + AcyI GRCGYC 1133 1135 . . 1132 1137 + AatII GACGTC 1136 1132 . . 1133 1136 + TaiI ACGT 1136 1132 . . 1133 1136 + SetI ASST 1136 1132 . . 1133 1136 + MaeII ACGT 1133 1135 . . 1143 1146 + TaqI TCGA 1143 1145 . . 1140 1144 - MboII GAAGA 1132 1131 . . 1146 1149 + SetI ASST 1149 1145 . . 1147 1151 + HphI GGTGA 1159 1158 . . 1145 1148 - MnlI CCTC 1138 1137 . . 1157 1160 + TaqI TCGA 1157 1159 . . 1164 1167 + CviJI RGCY 1165 1165 . . 1169 1172 + CviJI RGCY 1170 1170 . . 1171 1175 + BssKI CCNGG 1170 1175 . . 1171 1175 + BseBI CCWGG 1172 1173 . . 1171 1175 + ScrFI CCNGG 1172 1173 . . 1171 1175 + EcoRII CCWGG 1170 1175 . . 1173 1176 + SetI ASST 1176 1172 . . 1174 1180 + BstEII GGTNACC 1174 1179 . . 1174 1178 + HphI GGTGA 1186 1185 . . 1175 1179 + Tsp45I GTSAC 1174 1179 . . 1175 1179 + MaeIII GTNAC 1174 1179 . . 1178 1182 + Tsp4CI* ACNGT 1180 1179 . . 1183 1186 + CviRI* TGCA 1184 1184 . . 1186 1191 + ApoI RAATTY 1186 1190 . . 1187 1190 + TspEI AATT 1186 1190 . . 1203 1206 + TspEI AATT 1202 1206 . . 1210 1213 + Csp6I GTAC 1210 1212 . . 1210 1213 + RsaI GTAC 1211 1211 . . 1212 1215 + SetI ASST 1215 1211 . . 1219 1223 + MaeIII GTNAC 1218 1223 . . 1225 1230 + MfeI CAATTG 1225 1229 . . 1226 1229 + TspEI AATT 1225 1229 . . 1232 1235 + CviJI RGCY 1233 1233 . . 1236 1242 + SexAI ACCWGGT 1236 1241 . . 1237 1241 + BssKI CCNGG 1236 1241 . . 1237 1241 + BseBI CCWGG 1238 1239 . . 1237 1241 + ScrFI CCNGG 1238 1239 . . 1237 1241 + EcoRII CCWGG 1236 1241 . . 1239 1242 + SetI ASST 1242 1238 . . 1247 1250 + CviJI RGCY 1248 1248 . . 1268 1271 + MaeI CTAG 1268 1270 . . 1272 1275 + SetI ASST 1275 1271 . . 1271 1274 - MnlI CCTC 1264 1263 . . 1278 1283 + AsuII TTCGAA 1279 1281 . . 1279 1282 + TaqI TCGA 1279 1281 . . 1281 1285 + MboII GAAGA 1293 1292 . . 1281 1286 + BbvII* GAAGAC 1288 1292 . . 1289 1294 + Eco57MI CTGRAG 1310 1308 . . 1289 1294 + Eco57I CTGAAG 1310 1308 . . 1293 1296 + SetI ASST 1296 1292 . . 1291 1295 - Hin4II* CCTTC 1285 1284 . . 1309 1313 + MaeIII GTNAC 1308 1313 . . 1312 1316 + BsrI ACTGG 1317 1315 . . 1316 1321 + Hpy166II GTNNAC 1318 1318 . . 1335 1340 + Hpy178III* TCNNGA 1336 1338 . . 1351 1361 + FalI AAGNNNNNCTT 1342 1337 1374 1369 1357 1360 + SetI ASST 1360 1356 . . 1357 1360 + CviJI RGCY 1358 1358 . . 1357 1360 + AluI AGCT 1358 1358 . . 1351 1361 - FalI AAGNNNNNCTT 1374 1369 1342 1337 1366 1371 + NspI RCATGY 1370 1366 . . 1366 1371 + Cac8I GCNNGC 1368 1368 . . 1366 1371 + SphI GCATGC 1370 1366 . . 1367 1370 + FatI CATG 1366 1370 . . 1367 1370 + NlaIII CATG 1370 1366 . . 1367 1370 + CviAII CATG 1367 1369 . . 1372 1375 + FatI CATG 1371 1375 . . 1372 1375 + NlaIII CATG 1375 1371 . . 1372 1375 + CviAII CATG 1372 1374 . . 1387 1391 + BinI* GGATC 1395 1396 . . 1388 1391 + DpnI GATC 1389 1389 . . 1388 1391 + BstKTI GATC 1390 1388 . . 1388 1391 + MboI GATC 1387 1391 . . 1401 1404 + CviRI* TGCA 1402 1402 . . 1408 1411 + CviJI RGCY 1409 1409 . . 1416 1419 + CviRI* TGCA 1417 1417 . . 1414 1418 - SfaNI GCATC 1404 1408 . . 1419 1424 + ApoI RAATTY 1419 1423 . . 1420 1423 + TspEI AATT 1419 1423 . . 1444 1449 + SfeI* CTRYAG 1444 1448 . . 1452 1455 + CviJI RGCY 1453 1453 . . 1453 1457 + BlsI GCNGC 1455 1454 . . 1453 1457 + BisI GCNGC 1454 1455 . . 1449 1453 - BccI CCATC 1443 1444 . . 1453 1457 + TseI GCWGC 1453 1456 . . 1453 1457 - BbvI GCAGC 1440 1444 . . 1459 1462 + TspEI AATT 1458 1462 . . 1467 1470 + SetI ASST 1470 1466 . . 1467 1470 + CviJI RGCY 1468 1468 . . 1467 1470 + AluI AGCT 1468 1468 . . 1477 1482 + Hpy166II GTNNAC 1479 1479 . . 1477 1482 + BssNAI GTATAC 1479 1479 . . 1477 1482 + AccI GTMKAC 1478 1480 . . 1490 1494 + TspDTI ATGAA 1505 1503 . . 1491 1502 + BdaI TGANNNNNNTCA 1480 1478 1514 1512 1498 1501 + DpnI GATC 1499 1499 . . 1498 1501 + BstKTI GATC 1500 1498 . . 1498 1501 + MboI GATC 1497 1501 . . 1500 1505 + Hpy178III* TCNNGA 1501 1503 . . 1491 1502 - BdaI TGANNNNNNTCA 1514 1512 1480 1478 1511 1516 + Hpy178III* TCNNGA 1512 1514 . . 1517 1521 + Tsp4CI* ACNGT 1519 1518 . . 1528 1532 + Hpy188I TCNGA 1530 1529 . . 1532 1536 + TspDTI ATGAA 1547 1545 . . 1544 1547 + SetI ASST 1547 1543 . . 1544 1547 + CviJI RGCY 1545 1545 . . 1544 1547 + AluI AGCT 1545 1545 . . 1546 1551 + Eco57MI CTGRAG 1567 1565 . . 1546 1551 + Eco57I CTGAAG 1567 1565 . . 1548 1552 - Hin4II* CCTTC 1542 1541 . . 1563 1567 + FokI GGATG 1576 1580 . . 1563 1567 + BseGI GGATG 1569 1567 . . 1565 1569 + TspDTI ATGAA 1580 1578 . . 1570 1579 + MslI CAYNNNNRTG 1574 1574 . . 1583 1586 + TspEI AATT 1582 1586 . . 1589 1593 + Hpy188I TCNGA 1591 1590 . . 1591 1596 + XhoII RGATCY 1591 1595 . . 1591 1596 + BglII AGATCT 1591 1595 . . 1592 1595 + DpnI GATC 1593 1593 . . 1592 1595 + BstKTI GATC 1594 1592 . . 1592 1595 + MboI GATC 1591 1595 . . 1604 1609 + PvuII CAGCTG 1606 1606 . . 1604 1609 + NspBII* CMGCKG 1606 1606 . . 1605 1608 + SetI ASST 1608 1604 . . 1605 1608 + CviJI RGCY 1606 1606 . . 1605 1608 + AluI AGCT 1606 1606 . . 1601 1606 - Eco57MI CTGRAG 1586 1584 . . 1601 1606 - GsuI CTGGAG 1586 1584 . . 1614 1619 + BalI TGGCCA 1616 1616 . . 1614 1619 + CfrI YGGCCR 1614 1618 . . 1615 1618 + CviJI RGCY 1616 1616 . . 1615 1618 + HaeIII GGCC 1616 1616 . . 1618 1621 + FatI CATG 1617 1621 . . 1618 1621 + NlaIII CATG 1621 1617 . . 1618 1621 + CviAII CATG 1618 1620 . . 1619 1623 + TspDTI ATGAA 1634 1632 . . 1623 1626 + CviJI RGCY 1624 1624 . . 1624 1628 - BceAI ACGGC 1609 1611 . . 1633 1643 + FalI AAGNNNNNCTT 1624 1619 1656 1651 1632 1636 - Hin4II* CCTTC 1626 1625 . . 1633 1643 - FalI AAGNNNNNCTT 1656 1651 1624 1619 1644 1654 + MwoI GCNNNNNNNGC 1650 1647 . . 1647 1651 + Hpy188I TCNGA 1649 1648 . . 1645 1650 - Eco57MI CTGRAG 1630 1628 . . 1645 1650 - Eco57I CTGAAG 1630 1628 . . 1650 1654 - SfaNI GCATC 1640 1644 . . 1664 1668 - TspDTI ATGAA 1654 1652 . . 1672 1677 + TatI WGTACW 1672 1676 . . 1672 1677 + ScaI AGTACT 1674 1674 . . 1673 1676 + Csp6I GTAC 1673 1675 . . 1673 1676 + RsaI GTAC 1674 1674 . . 1675 1679 + Tsp4CI* ACNGT 1677 1676 . . 1676 1681 + SfeI* CTRYAG 1676 1680 . . 1683 1686 + SetI ASST 1686 1682 . . 1681 1685 - Hin4II* CCTTC 1675 1674 . . 1688 1691 + MseI TTAA 1688 1690 . . 1693 1696 + MseI TTAA 1693 1695 . . 1697 1702 + BseRI GAGGAG 1712 1710 . . 1697 1700 - MnlI CCTC 1690 1689 . . 1702 1707 + EcoRV GATATC 1704 1704 . . 1697 1707 - Hin4I GAYNNNNNVTC 1720 1715 1688 1683 1723 1727 + HphI GGTGA 1735 1734 . . 1726 1729 + DpnI GATC 1727 1727 . . 1726 1729 + BstKTI GATC 1728 1726 . . 1726 1729 + MboI GATC 1725 1729 . . 1730 1733 + MnlI CCTC 1740 1739 . . 1726 1730 - BinI* GGATC 1720 1721 . . 1739 1743 + DdeI CTNAG 1739 1742 . . 1743 1747 + BsmAI GTCTC 1748 1752 . . 1748 1751 + TspEI AATT 1747 1751 . . 1752 1757 + AhaIII* TTTAAA 1754 1754 . . 1753 1756 + MseI TTAA 1753 1755 . . 1759 1764 + SnaBI TACGTA 1761 1761 . . 1759 1764 + BsaAI YACGTR 1761 1761 . . 1760 1763 + TaiI ACGT 1763 1759 . . 1760 1763 + SetI ASST 1763 1759 . . 1760 1763 + MaeII ACGT 1760 1762 . . 1773 1776 + CviRI* TGCA 1774 1774 . . 1781 1784 + DpnI GATC 1782 1782 . . 1781 1784 + BstKTI GATC 1783 1781 . . 1781 1784 + MboI GATC 1780 1784 . . 1786 1791 + Hpy166II GTNNAC 1788 1788 . . 1795 1800 + BetI* WCCGGW 1795 1799 . . 1796 1799 + HpaII CCGG 1796 1798 . . 1802 1807 + StuI AGGCCT 1804 1804 . . 1803 1806 + CviJI RGCY 1804 1804 . . 1803 1806 + HaeIII GGCC 1804 1804 . . 1804 1814 + MwoI GCNNNNNNNGC 1810 1807 . . 1804 1814 + BglI GCCNNNNNGGC 1810 1807 . . 1805 1808 + MnlI CCTC 1815 1814 . . 1808 1812 + Hpy99I CGWCG 1812 1807 . . 1812 1815 + CviJI RGCY 1813 1813 . . 1813 1817 + BlsI GCNGC 1815 1814 . . 1813 1817 + BisI GCNGC 1814 1815 . . 1813 1817 + TseI GCWGC 1813 1816 . . 1813 1817 - BbvI GCAGC 1800 1804 . . 1814 1819 - EcoP15I CAGCAG 1786 1788 . . 1822 1825 + MseI TTAA 1822 1824 . . 1826 1831 + PvuII CAGCTG 1828 1828 . . 1826 1831 + NspBII* CMGCKG 1828 1828 . . 1827 1830 + SetI ASST 1830 1826 . . 1827 1830 + CviJI RGCY 1828 1828 . . 1827 1830 + AluI AGCT 1828 1828 . . 1828 1832 + BlsI GCNGC 1830 1829 . . 1828 1832 + BisI GCNGC 1829 1830 . . 1828 1832 + TseI GCWGC 1828 1831 . . 1828 1832 - BbvI GCAGC 1815 1819 . . 1831 1834 - AciI CCGC 1831 1833 . . 1846 1849 - MnlI CCTC 1839 1838 . . 1849 1853 - Hin4II* CCTTC 1843 1842 . . 1854 1857 + SetI ASST 1857 1853 . . 1855 1859 + HphI GGTGA 1867 1866 . . 1853 1856 - MnlI CCTC 1846 1845 . . 1865 1870 + MfeI CAATTG 1865 1869 . . 1866 1869 + TspEI AATT 1865 1869 . . 1875 1878 + SetI ASST 1878 1874 . . 1873 1878 - BspMI ACCTGC 1864 1868 . . 1873 1879 - AarI CACCTGC 1864 1868 . . 1882 1885 + MseI TTAA 1882 1884 . . 1887 1892 + Cac8I GCNNGC 1889 1889 . . 1898 1902 + Tsp4CI* ACNGT 1900 1899 . . 1912 1917 + ClaI ATCGAT 1913 1915 . . 1913 1916 + TaqI TCGA 1913 1915 . . 1916 1920 + TspDTI ATGAA 1931 1929 . . 1918 1923 + ApoI RAATTY 1918 1922 . . 1919 1922 + TspEI AATT 1918 1922 . . 1939 1943 + Hpy188I TCNGA 1941 1940 . . 1942 1945 + DpnI GATC 1943 1943 . . 1942 1945 + BstKTI GATC 1944 1942 . . 1942 1945 + MboI GATC 1941 1945 . . 1956 1961 + Hpy178III* TCNNGA 1957 1959 . . 1956 1961 + BspHI TCATGA 1956 1960 . . 1957 1960 + FatI CATG 1956 1960 . . 1957 1960 + NlaIII CATG 1960 1956 . . 1957 1960 + CviAII CATG 1957 1959 . . 1958 1962 + TspDTI ATGAA 1973 1971 . . 1955 1959 - TspDTI ATGAA 1945 1943 . . 1962 1965 + SetI ASST 1965 1961 . . 1962 1965 + CviJI RGCY 1963 1963 . . 1962 1965 + AluI AGCT 1963 1963 . . 1979 1983 + BccI CCATC 1987 1988 . . 1995 1998 + SetI ASST 1998 1994 . . 1995 1998 + CviJI RGCY 1996 1996 . . 1995 1998 + AluI AGCT 1996 1996 . . 2013 2018 + AhaIII* TTTAAA 2015 2015 . . 2014 2017 + MseI TTAA 2014 2016 . . 2019 2022 + CviRI* TGCA 2020 2020 . . 2031 2034 + TspEI AATT 2030 2034 . . 2035 2038 + MaeI CTAG 2035 2037 . . 2038 2042 + TauI GCSGC 2041 2038 . . 2038 2042 + BlsI GCNGC 2040 2039 . . 2038 2042 + BisI GCNGC 2039 2040 . . 2040 2043 + CviJI RGCY 2041 2041 . . 2041 2045 + BlsI GCNGC 2043 2042 . . 2041 2045 + BisI GCNGC 2042 2043 . . 2041 2045 + TseI GCWGC 2041 2044 . . 2038 2041 - AciI CCGC 2038 2040 . . 2041 2045 - BbvI GCAGC 2028 2032 . . 2049 2053 + BssKI CCNGG 2048 2053 . . 2049 2053 + ScrFI CCNGG 2050 2051 . . 2049 2053 + CauII* CCSGG 2050 2051 . . 2050 2053 + HpaII CCGG 2050 2052 . . 2050 2060 + BsiYI* CCNNNNNNNGG 2056 2053 . . 2055 2058 + SetI ASST 2058 2054 . . 2060 2064 + MboII GAAGA 2072 2071 . . 2081 2087 + TspRI CASTGNN 2087 2078 . . 2083 2087 + DdeI CTNAG 2083 2086 . . 2083 2087 - BspCNI CTCAG 2075 2073 . . 2083 2087 - BseMII CTCAG 2074 2072 . . 2085 2088 - MnlI CCTC 2078 2077 . . 2092 2095 + TspEI AATT 2091 2095 . . 2093 2100 + SwaI ATTTAAAT 2096 2096 . . 2094 2099 + AhaIII* TTTAAA 2096 2096 . . 2095 2098 + MseI TTAA 2095 2097 . . 2105 2108 + AciI CCGC 2105 2107 . . 2112 2115 + DpnI GATC 2113 2113 . . 2112 2115 + BstKTI GATC 2114 2112 . . 2112 2115 + MboI GATC 2111 2115 . . 2115 2118 + FatI CATG 2114 2118 . . 2115 2118 + NlaIII CATG 2118 2114 . . 2115 2118 + CviAII CATG 2115 2117 . . 2119 2124 + Hpy178III* TCNNGA 2120 2122 . . 2145 2150 + Hpy178III* TCNNGA 2146 2148 . . 2153 2157 + Tsp4CI* ACNGT 2155 2154 . . 2156 2160 + MaeIII GTNAC 2155 2160 . . 2166 2169 + TspEI AATT 2165 2169 . . 2177 2180 + TspEI AATT 2176 2180 . . 2182 2186 + SfaNI GCATC 2191 2195 . . 2194 2199 + Hpy166II GTNNAC 2196 2196 . . 2194 2199 + HindII GTYRAC 2196 2196 . . 2205 2208 + FatI CATG 2204 2208 . . 2205 2208 + NlaIII CATG 2208 2204 . . 2205 2208 + CviAII CATG 2205 2207 . . 2206 2210 + TspDTI ATGAA 2221 2219 . . 2213 2217 - BsmAI GTCTC 2207 2211 . . 2213 2218 - Esp3I CGTCTC 2207 2211 . . 2220 2223 + CviJI RGCY 2221 2221 . . 2228 2231 + CviJI RGCY 2229 2229 . . 2229 2233 + TauI GCSGC 2232 2229 . . 2229 2233 + BlsI GCNGC 2231 2230 . . 2229 2233 + BisI GCNGC 2230 2231 . . 2230 2233 + AciI CCGC 2230 2232 . . 2240 2245 + BsgI GTGCAG 2261 2259 . . 2241 2244 + CviRI* TGCA 2242 2242 . . 2253 2256 + TaiI ACGT 2256 2252 . . 2253 2256 + SetI ASST 2256 2252 . . 2253 2256 + MaeII ACGT 2253 2255 . . 2254 2258 + Hpy99I CGWCG 2258 2253 . . 2271 2274 + CviRI* TGCA 2272 2272 . . 2273 2278 + BsaAI YACGTR 2275 2275 . . 2274 2277 + TaiI ACGT 2277 2273 . . 2274 2277 + SetI ASST 2277 2273 . . 2274 2277 + MaeII ACGT 2274 2276 . . 2276 2279 + Csp6I GTAC 2276 2278 . . 2276 2279 + RsaI GTAC 2277 2277 . . 2281 2286 + AhaIII* TTTAAA 2283 2283 . . 2282 2285 + MseI TTAA 2282 2284 . . 2289 2294 + SspI AATATT 2291 2291 . . 2293 2296 + MseI TTAA 2293 2295 . . 2304 2307 + CviJI RGCY 2305 2305 . . 2321 2324 + TaqI TCGA 2321 2323 . . 2321 2326 + Hpy178III* TCNNGA 2322 2324 . . 2338 2342 + DdeI CTNAG 2338 2341 . . 2338 2342 - BspCNI CTCAG 2330 2328 . . 2338 2342 - BseMII CTCAG 2329 2327 . . 2346 2350 + FokI GGATG 2359 2363 . . 2346 2350 + BseGI GGATG 2352 2350 . . 2354 2360 + PasI CCCWGGG 2355 2358 . . 2350 2354 - SfaNI GCATC 2340 2344 . . 2354 2359 + SecI* CCNNGG 2354 2358 . . 2355 2359 + BssKI CCNGG 2354 2359 . . 2355 2359 + BseBI CCWGG 2356 2357 . . 2355 2360 + SecI* CCNNGG 2355 2359 . . 2355 2359 + ScrFI CCNGG 2356 2357 . . 2355 2359 + EcoRII CCWGG 2354 2359 . . 2369 2372 + DpnI GATC 2370 2370 . . 2369 2372 + BstKTI GATC 2371 2369 . . 2369 2372 + MboI GATC 2368 2372 . . 2371 2374 + TaqI TCGA 2371 2373 . . 2381 2385 + TfiI GAWTC 2381 2384 . . 2381 2385 + HinfI GANTC 2381 2384 . . 2386 2390 + Tsp4CI* ACNGT 2388 2387 . . 2419 2423 + Hpy188I TCNGA 2421 2420 . . 2424 2427 + SetI ASST 2427 2423 . . 2424 2427 + CviJI RGCY 2425 2425 . . 2424 2427 + AluI AGCT 2425 2425 . . 2433 2436 - MnlI CCTC 2426 2425 . . 2436 2439 + CviJI RGCY 2437 2437 . . 2440 2443 + TspEI AATT 2439 2443 . . 2447 2450 + TaqI TCGA 2447 2449 . . 2450 2454 + TspDTI ATGAA 2465 2463 . . 2454 2457 + TspEI AATT 2453 2457 . . 2461 2465 + BccI CCATC 2469 2470 . . 2467 2471 - TspDTI ATGAA 2457 2455 . . 2472 2475 + CviRI* TGCA 2473 2473 . . 2469 2474 - BsrDI GCAATG 2468 2466 . . 2477 2482 + HindIII AAGCTT 2477 2481 . . 2478 2481 + SetI ASST 2481 2477 . . 2478 2481 + CviJI RGCY 2479 2479 . . 2478 2481 + AluI AGCT 2479 2479 . . 2485 2488 + DpnI GATC 2486 2486 . . 2485 2488 + BstKTI GATC 2487 2485 . . 2485 2488 + MboI GATC 2484 2488 . . 2491 2494 + MseI TTAA 2491 2493 . . 2520 2524 + FokI GGATG 2533 2537 . . 2520 2524 + BseGI GGATG 2526 2524 . . 2535 2539 + FokI GGATG 2548 2552 . . 2535 2539 + BseGI GGATG 2541 2539 . . 2537 2541 + TspDTI ATGAA 2552 2550 . . 2539 2543 + MboII GAAGA 2551 2550 . . 2542 2547 + ApoI RAATTY 2542 2546 . . 2543 2546 + TspEI AATT 2542 2546 . . 2559 2563 + PleI GAGTC 2567 2568 . . 2559 2563 + MlyI GAGTC 2568 2568 . . 2559 2563 + HinfI GANTC 2559 2562 . . 2567 2571 + Tsp45I GTSAC 2566 2571 . . 2567 2571 + MaeIII GTNAC 2566 2571 . . 2559 2569 - Hin4I GAYNNNNNVTC 2582 2577 2550 2545 2572 2576 + TauI GCSGC 2575 2572 . . 2572 2576 + BlsI GCNGC 2574 2573 . . 2572 2576 + BisI GCNGC 2573 2574 . . 2573 2576 + AciI CCGC 2573 2575 . . 2578 2583 + Hpy178III* TCNNGA 2579 2581 . . 2605 2609 + BslFI GGGAC 2619 2623 . . 2602 2606 - BccI CCATC 2596 2597 . . 2607 2615 + Tth111I GACNNNGTC 2610 2611 . . 2608 2612 + BsrI ACTGG 2613 2611 . . 2608 2613 + BfiI ACTGGG 2618 2617 . . 2616 2619 + SetI ASST 2619 2615 . . 2622 2625 + TspEI AATT 2621 2625 . . 2633 2636 + CviJI RGCY 2634 2634 . . 2639 2644 + EcoP15I CAGCAG 2669 2671 . . 2650 2654 + MboII GAAGA 2662 2661 . . 2653 2657 - Hin4II* CCTTC 2647 2646 . . 2667 2670 + SetI ASST 2670 2666 . . 2667 2670 + CviJI RGCY 2668 2668 . . 2667 2670 + AluI AGCT 2668 2668 . . 2669 2674 + SfeI* CTRYAG 2669 2673 . . 2673 2676 + SetI ASST 2676 2672 . . 2673 2676 + CviJI RGCY 2674 2674 . . 2673 2676 + AluI AGCT 2674 2674 . . 2680 2684 + BssKI CCNGG 2679 2684 . . 2680 2684 + BseBI CCWGG 2681 2682 . . 2680 2684 + ScrFI CCNGG 2681 2682 . . 2680 2684 + EcoRII CCWGG 2679 2684 . . 2682 2685 + SetI ASST 2685 2681 . . 2684 2687 + Csp6I GTAC 2684 2686 . . 2684 2687 + RsaI GTAC 2685 2685 . . 2694 2698 + MboII GAAGA 2706 2705 . . 2697 2701 + MboII GAAGA 2709 2708 . . 2707 2711 + Tsp4CI* ACNGT 2709 2708 . . 2709 2713 + HphI GGTGA 2721 2720 . . 2710 2714 + Tsp45I GTSAC 2709 2714 . . 2710 2714 + MaeIII GTNAC 2709 2714 . . 2713 2716 + TaiI ACGT 2716 2712 . . 2713 2716 + SetI ASST 2716 2712 . . 2713 2716 + MaeII ACGT 2713 2715 . . 2733 2736 + FatI CATG 2732 2736 . . 2733 2736 + NlaIII CATG 2736 2732 . . 2733 2736 + CviAII CATG 2733 2735 . . 2737 2741 + TspDTI ATGAA 2752 2750 . . 2742 2745 + FatI CATG 2741 2745 . . 2742 2745 + NlaIII CATG 2745 2741 . . 2742 2745 + CviAII CATG 2742 2744 . . 2745 2748 + DpnI GATC 2746 2746 . . 2745 2748 + BstKTI GATC 2747 2745 . . 2745 2748 + MboI GATC 2744 2748 . . 2762 2767 + Eco57MI CTGRAG 2783 2781 . . 2762 2767 + Eco57I CTGAAG 2783 2781 . . 2776 2779 - MnlI CCTC 2769 2768 . . 2780 2785 + BsgI GTGCAG 2801 2799 . . 2781 2784 + CviRI* TGCA 2782 2782 . . 2785 2789 + MboII GAAGA 2797 2796 . . 2795 2800 + PvuII CAGCTG 2797 2797 . . 2795 2800 + NspBII* CMGCKG 2797 2797 . . 2796 2799 + SetI ASST 2799 2795 . . 2796 2799 + CviJI RGCY 2797 2797 . . 2796 2799 + AluI AGCT 2797 2797 . . 2829 2833 - Hin4II* CCTTC 2823 2822 . . 2858 2863 + SspI AATATT 2860 2860 . . 2866 2870 + MboII GAAGA 2878 2877 . . 2866 2871 - Ksp632I* CTCTTC 2861 2864 . . 2873 2877 + MboII GAAGA 2885 2884 . . 2899 2902 + SetI ASST 2902 2898 . . 2920 2923 + MseI TTAA 2920 2922 . . 2928 2932 + TfiI GAWTC 2928 2931 . . 2928 2932 + HinfI GANTC 2928 2931 . . 2932 2935 + FatI CATG 2931 2935 . . 2932 2935 + NlaIII CATG 2935 2931 . . 2932 2935 + CviAII CATG 2932 2934 . . 2930 2934 - TspDTI ATGAA 2920 2918 . . 2935 2940 + KpnI GGTACC 2939 2935 . . 2935 2940 + NlaIV GGNNCC 2937 2937 . . 2935 2940 + HgiCI* GGYRCC 2935 2939 . . 2935 2940 + Acc65I GGTACC 2935 2939 . . 2936 2939 + Csp6I GTAC 2936 2938 . . 2936 2939 + RsaI GTAC 2937 2937 . . 2947 2950 + TspEI AATT 2946 2950 . . 2950 2953 + MseI TTAA 2950 2952 . . 2955 2960 + XhoII RGATCY 2955 2959 . . 2955 2960 + BglII AGATCT 2955 2959 . . 2956 2959 + DpnI GATC 2957 2957 . . 2956 2959 + BstKTI GATC 2958 2956 . . 2956 2959 + MboI GATC 2955 2959 . . 2958 2963 + Hpy178III* TCNNGA 2959 2961 . . 2959 2964 + SmlI CTYRAG 2959 2963 . . 2959 2964 + Bce83I* CTTGAG 2980 2978 . . 2968 2971 - MnlI CCTC 2961 2960 . . 2975 2979 + TspDTI ATGAA 2990 2988 . . 2989 2993 + Tsp4CI* ACNGT 2991 2990 . . 3002 3006 - TspDTI ATGAA 2992 2990 . . 3004 3008 - FokI GGATG 2990 2994 . . 3004 3008 - BseGI GGATG 3003 3001 . . 3011 3015 + Tsp4CI* ACNGT 3013 3012 . . 3017 3020 + SetI ASST 3020 3016 . . 3016 3019 - MnlI CCTC 3009 3008 . . 3024 3028 + BinI* GGATC 3032 3033 . . 3025 3028 + DpnI GATC 3026 3026 . . 3025 3028 + BstKTI GATC 3027 3025 . . 3025 3028 + MboI GATC 3024 3028 . . 3028 3033 + MfeI CAATTG 3028 3032 . . 3029 3032 + TspEI AATT 3028 3032 . . 3033 3038 + NlaIV GGNNCC 3035 3035 . . 3043 3047 + TfiI GAWTC 3043 3046 . . 3043 3047 + HinfI GANTC 3043 3046 . . 3049 3052 + SetI ASST 3052 3048 . . 3049 3052 + CviJI RGCY 3050 3050 . . 3049 3052 + AluI AGCT 3050 3050 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 1190 1864 # # AatII: # [GACGTC] # 1136 1918 # # Acc65I: # [GGTACC] # 119 2935 # # AccI: # [GTMKAC] # 681 797 1576 # # AciI: # [CCGC] # 40 53 58 67 70 82 # 125 207 343 404 413 481 # 711 # # AcyI: # [GRCGYC] # 1133 1921 # # AflIII: # [ACRYGT] # 148 780 2126 # # AhaIII*: # [TTTAAA] # 81 187 261 771 1754 # # AluI: # [AGCT] # 4 6 14 19 33 54 # 61 77 87 110 123 126 # 135 189 222 253 253 424 # 429 435 # # ApoI: # [RAATTY] # 107 233 255 403 421 499 # 512 624 # # AsuI*: # [GGNCC] # 175 722 2157 # # AsuII: # [TTCGAA] # 1279 1775 # # AvaII: # [GGWCC] # 897 2157 # # BalI: # [TGGCCA] # 1438 1616 # # BarI: # [GAAGNNNNNNTAC] # 27 32 2995 # # BbvI: # [GCAGC] # 15 213 360 1026 1440 # # BbvII*: # [GAAGAC] # 365 923 1766 # # BccI: # [CCATC] # 72 127 148 429 458 482 # 544 794 # # Bce83I*: # [CTTGAG] # 74 2980 # # BceAI: # [ACGGC] # 683 926 1445 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 613 833 1540 # # BetI*: # [WCCGGW] # 1259 1795 # # BfiI: # [ACTGGG] # 436 2618 # # BglI: # [GCCNNNNNGGC] # 1244 1810 # # BglII: # [AGATCT] # 99 753 838 1364 # # BinI*: # [GGATC] # 22 97 325 338 352 608 # 1312 # # BisI: # [GCNGC] # 3 15 188 210 334 343 # 360 413 481 707 # # BlsI: # [GCNGC] # 3 15 188 210 334 343 # 360 413 480 708 # # BmgT120I: # [GGNCC] # 176 722 2156 # # BsaAI: # [YACGTR] # 514 779 832 929 # # BseBI: # [CCWGG] # 66 119 325 340 373 713 # 1118 # # BseGI: # [GGATG] # 4 15 51 174 462 641 # 783 924 # # BseMII: # [CTCAG] # 211 255 313 360 725 1190 # # BseRI: # [GAGGAG] # 42 1342 1670 # # BsgI: # [GTGCAG] # 253 540 2261 # # BsiYI*: # [CCNNNNNNNGG] # 5 713 998 1338 # # BslFI: # [GGGAC] # 153 435 735 1731 # # BsmAI: # [GTCTC] # 459 847 1748 # # BspCNI: # [CTCAG] # 210 255 313 362 724 1190 # # BspHI: # [TCATGA] # 1098 1956 # # BspMI: # [ACCTGC] # 270 1190 1594 # # BsrDI: # [GCAATG] # 586 2468 # # BsrI: # [ACTGG] # 66 324 441 927 1296 # # BssKI: # [CCNGG] # 66 119 306 325 340 375 # 711 812 # # BssNAI: # [GTATAC] # 1479 1575 # # BstEII: # [GGTNACC] # 1174 1880 # # BstKTI: # [GATC] # 27 55 69 84 94 110 # 116 134 141 161 170 210 # 211 257 260 339 616 # # Cac8I: # [GCNNGC] # 81 447 521 840 1165 # # CauII*: # [CCSGG] # 1004 2050 # # CfrI: # [YGGCCR] # 1440 1614 # # ClaI: # [ATCGAT] # 1141 1913 # # Csp6I: # [GTAC] # 86 118 146 252 408 463 # 603 978 # # CviAII: # [CATG] # 5 9 90 106 122 154 # 158 190 246 251 255 278 # 323 339 528 # # CviJI: # [RGCY] # 4 5 6 6 8 8 # 9 10 12 14 15 15 # 15 19 33 34 36 42 # 44 45 51 53 57 61 # 63 64 76 77 87 88 # 96 101 110 120 123 126 # 135 155 180 180 201 217 # 253 # # CviRI*: # [TGCA] # 15 30 201 218 219 222 # 246 272 309 357 358 607 # # DdeI: # [CTNAG] # 197 255 313 344 383 716 # 846 # # DpnI: # [GATC] # 28 55 69 84 94 110 # 116 134 141 161 170 210 # 211 257 260 339 615 # # DraII: # [RGGNCCY] # 175 2879 # # DsaI*: # [CCRYGG] # 731 2323 # # EciI: # [GGCGGA] # 47 3007 # # Ecl136II: # [GAGCTC] # 412 2642 # # Eco57I: # [CTGAAG] # 63 257 271 1153 1310 # # Eco57MI: # [CTGRAG] # 19 44 257 271 1153 1310 # # EcoP15I: # [CAGCAG] # 385 883 1786 # # EcoRI: # [GAATTC] # 255 824 1975 # # EcoRII: # [CCWGG] # 66 119 325 340 375 711 # 1118 # # EcoRV: # [GATATC] # 1350 1704 # # Esp3I: # [CGTCTC] # 847 2207 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 0 0 32 32 32 32 # 250 270 403 605 1398 # # FatI: # [CATG] # 5 9 90 106 123 153 # 158 190 246 251 255 278 # 323 339 528 # # FnuDII*: # [CGCG] # 235 887 1932 # # FokI: # [GGATG] # 15 64 174 442 652 783 # 924 # # GsuI: # [CTGGAG] # 1468 1586 # # HaeIII: # [GGCC] # 176 188 1250 1440 # # HgiAI*: # [GWGCWC] # 81 414 2559 # # HgiCI*: # [GGYRCC] # 119 2935 # # HgiJII*: # [GRGCYC] # 414 2640 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 32 32 109 472 682 830 # 833 # # Hin4II*: # [CCTTC] # 9 49 53 84 168 176 # 231 257 304 359 560 804 # # HindII: # [GTYRAC] # 186 858 2010 # # HindIII: # [AAGCTT] # 270 577 651 1556 # # HinfI: # [GANTC] # 5 11 13 77 84 101 # 101 115 178 310 369 1690 # # HpaI: # [GTTAAC] # 186 2868 # # HpaII: # [CCGG] # 254 1004 1796 # # HphI: # [GGTGA] # 27 66 121 132 234 333 # 334 404 549 854 # # Hpy166II: # [GTNNAC] # 37 124 137 161 186 309 # 338 408 496 858 # # Hpy178III*: # [TCNNGA] # 11 24 26 75 95 134 # 156 163 165 176 252 257 # 380 445 695 # # Hpy188I: # [TCNGA] # 51 58 61 88 105 184 # 225 292 425 452 480 633 # # Hpy99I: # [CGWCG] # 72 446 796 1740 # # KpnI: # [GGTACC] # 115 2939 # # Ksp632I*: # [CTCTTC] # 52 193 552 2257 # # MaeI: # [CTAG] # 122 374 767 772 1019 # # MaeII: # [ACGT] # 18 20 21 31 148 174 # 296 341 439 446 493 627 # # MaeIII: # [GTNAC] # 24 44 90 118 125 133 # 139 143 162 190 283 345 # 411 847 # # MboI: # [GATC] # 30 55 69 84 94 110 # 116 134 141 161 170 210 # 211 257 260 339 613 # # MboII: # [GAAGA] # 3 7 34 44 52 81 # 88 111 134 137 161 169 # 199 236 340 479 779 # # MfeI: # [CAATTG] # 26 640 1163 1225 # # MlyI: # [GAGTC] # 92 95 486 500 1881 # # MmeI: # [TCCRAC] # 71 2983 # # MnlI: # [CCTC] # 7 24 45 48 50 63 # 75 126 154 180 192 232 # 250 343 348 426 491 # # MseI: # [TTAA] # 5 11 30 36 60 60 # 69 81 104 114 132 185 # 187 198 254 429 447 652 # # MslI: # [CAYNNNNRTG] # 1480 1574 # # MwoI: # [GCNNNNNNNGC] # 89 160 525 1036 1244 # # NcoI: # [CCATGG] # 731 2323 # # NlaIII: # [CATG] # 5 9 90 106 119 157 # 158 190 246 251 255 278 # 323 339 528 # # NlaIV: # [GGNNCC] # 19 98 231 882 1824 # # NspBII*: # [CMGCKG] # 19 222 257 969 1587 # # NspI: # [RCATGY] # 157 1213 1684 # # PasI: # [CCCWGGG] # 699 2355 # # PflMI: # [CCANNNNNTGG] # 718 2336 # # PleI: # [GAGTC] # 91 95 487 500 1881 # # PshAI: # [GACNNNNGTC] # 629 2425 # # PvuII: # [CAGCTG] # 19 222 257 969 1587 # # RsaI: # [GTAC] # 86 117 146 252 408 463 # 603 979 # # SacI: # [GAGCTC] # 414 2640 # # ScaI: # [AGTACT] # 1380 1674 # # ScrFI: # [CCNGG] # 66 119 306 325 340 373 # 713 812 # # SduI: # [GDGCHC] # 81 414 2559 # # SecI*: # [CCNNGG] # 1 19 699 712 1623 # # SetI: # [ASST] # 2 5 6 6 9 9 # 10 11 13 14 15 18 # 18 21 21 21 21 24 # 26 27 27 27 29 29 # 31 31 32 33 33 33 # 39 42 47 49 50 51 # 54 58 60 61 64 67 # 77 77 78 81 83 87 # 90 95 97 103 110 112 # 116 118 138 150 198 # # SexAI: # [ACCWGGT] # 1236 1818 # # SfaNI: # [GCATC] # 149 236 551 714 1404 # # SfeI*: # [CTRYAG] # 232 338 385 993 1106 # # SmlI: # [CTYRAG] # 95 2959 # # SnaBI: # [TACGTA] # 1293 1761 # # SphI: # [GCATGC] # 1370 1684 # # SplI*: # [CGTACG] # 1123 1931 # # SspI: # [AATATT] # 194 520 569 1771 # # StuI: # [AGGCCT] # 1250 1804 # # StyI: # [CCWWGG] # 731 2323 # # SwaI: # [ATTTAAAT] # 958 2096 # # TaiI: # [ACGT] # 18 20 21 31 148 174 # 299 338 439 446 493 627 # # TaqI: # [TCGA] # 14 34 50 70 76 122 # 240 332 408 467 607 634 # # TatI: # [WGTACW] # 695 977 1382 # # TauI: # [GCSGC] # 191 343 413 479 709 919 # # TfiI: # [GAWTC] # 11 84 101 106 115 400 # 547 1690 # # TseI: # [GCWGC] # 15 213 360 1013 1453 # # TsoI: # [TARCCA] # 493 2561 # # Tsp45I: # [GTSAC] # 24 118 139 143 295 345 # 598 1392 # # Tsp4CI*: # [ACNGT] # 22 41 63 89 158 212 # 223 233 255 282 321 339 # 816 # # TspDTI: # [ATGAA] # 2 8 14 20 26 28 # 33 42 54 62 70 87 # 168 170 200 214 236 248 # 272 277 823 # # TspEI: # [AATT] # 5 11 14 16 23 26 # 39 53 61 74 79 82 # 83 89 107 112 118 124 # 162 162 165 172 194 241 # 254 263 325 # # TspGWI: # [ACGGA] # 35 99 322 433 2165 # # TspRI: # [CASTGNN] # 103 384 500 967 1100 # # Tth111I: # [GACNNNGTC] # 444 2610 # # XhoII: # [RGATCY] # 99 210 225 543 613 1364 # # ZraI: # [GACGTC] # 1134 1920 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3054 # Reported_sequences: 1 # Reported_hitcount: 793 #---------------------------------------