Restriction Fragments for GIS1/YDR096W



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The currently selected gene/sequence is :

GIS1/YDR096W on chromosome IV from coordinates 637139 to 639823.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.31419.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 11 14 + CviJI RGCY 12 12 . . 13 17 - BsrI ACTGG 13 11 . . 20 23 + SetI ASST 23 19 . . 19 22 - MnlI CCTC 12 11 . . 28 32 - BccI CCATC 22 23 . . 36 41 + BetI* WCCGGW 36 40 . . 37 40 + HpaII CCGG 37 39 . . 44 47 + MseI TTAA 44 46 . . 47 50 + CviJI RGCY 48 48 . . 48 52 - BceAI ACGGC 33 35 . . 57 61 - BccI CCATC 51 52 . . 66 69 + CviRI* TGCA 67 67 . . 69 74 + ApoI RAATTY 69 73 . . 70 73 + TspEI AATT 69 73 . . 77 82 + SspI AATATT 79 79 . . 82 86 - TspDTI ATGAA 72 70 . . 89 93 + TspDTI ATGAA 104 102 . . 93 96 + TspEI AATT 92 96 . . 100 105 + ApoI RAATTY 100 104 . . 101 104 + TspEI AATT 100 104 . . 104 108 + Hpy188I TCNGA 106 105 . . 136 139 + MnlI CCTC 146 145 . . 140 154 + XcmI CCANNNNNNNNNTGG 147 146 . . 140 150 + BsiYI* CCNNNNNNNGG 146 143 . . 140 150 + PflMI CCANNNNNTGG 146 143 . . 149 152 + CviJI RGCY 150 150 . . 151 156 + Eco57MI CTGRAG 172 170 . . 151 156 + GsuI CTGGAG 172 170 . . 155 158 + SetI ASST 158 154 . . 155 158 + CviJI RGCY 156 156 . . 155 158 + AluI AGCT 156 156 . . 166 169 + CviJI RGCY 167 167 . . 163 167 - Hin4II* CCTTC 157 156 . . 171 174 + SetI ASST 174 170 . . 172 175 + MnlI CCTC 182 181 . . 169 173 - HphI GGTGA 161 160 . . 178 181 - AciI CCGC 178 180 . . 186 191 + AhaIII* TTTAAA 188 188 . . 187 190 + MseI TTAA 187 189 . . 202 205 + MaeI CTAG 202 204 . . 205 209 + HinfI GANTC 205 208 . . 205 209 - PleI GAGTC 199 200 . . 205 209 - MlyI GAGTC 199 199 . . 213 217 + TfiI GAWTC 213 216 . . 213 217 + HinfI GANTC 213 216 . . 225 228 + CviJI RGCY 226 226 . . 227 237 + BsiYI* CCNNNNNNNGG 233 230 . . 227 237 + PflMI CCANNNNNTGG 233 230 . . 231 236 + BtgZI GCGATG 246 250 . . 236 240 + BslFI GGGAC 250 254 . . 233 237 - BccI CCATC 227 228 . . 244 247 + FatI CATG 243 247 . . 244 247 + NlaIII CATG 247 243 . . 244 247 + CviAII CATG 244 246 . . 245 249 + TspDTI ATGAA 260 258 . . 251 255 + Tsp4CI* ACNGT 253 252 . . 263 267 + SfaNI GCATC 272 276 . . 266 269 + TaqI TCGA 266 268 . . 280 290 + Hin4I GAYNNNNNVTC 271 266 303 298 280 290 + Eam1105I GACNNNNNGTC 285 284 . . 280 290 - Hin4I GAYNNNNNVTC 303 298 271 266 291 294 + Csp6I GTAC 291 293 . . 291 294 + RsaI GTAC 292 292 . . 295 298 + TspEI AATT 294 298 . . 298 301 + MseI TTAA 298 300 . . 303 307 + Tsp4CI* ACNGT 305 304 . . 304 310 + TspRI CASTGNN 310 301 . . 309 313 + MboII GAAGA 321 320 . . 317 322 + CfrI YGGCCR 317 321 . . 318 321 + CviJI RGCY 319 319 . . 318 321 + HaeIII GGCC 319 319 . . 327 332 + XbaI TCTAGA 327 331 . . 327 332 + Hpy178III* TCNNGA 328 330 . . 328 331 + MaeI CTAG 328 330 . . 331 335 + HinfI GANTC 331 334 . . 334 339 + Hpy178III* TCNNGA 335 337 . . 331 335 - PleI GAGTC 325 326 . . 331 335 - MlyI GAGTC 325 325 . . 336 339 + TaqI TCGA 336 338 . . 348 351 + SetI ASST 351 347 . . 349 353 + HphI GGTGA 361 360 . . 356 359 + MseI TTAA 356 358 . . 369 374 + AhaIII* TTTAAA 371 371 . . 370 373 + MseI TTAA 370 372 . . 379 382 + TspEI AATT 378 382 . . 383 387 + TauI GCSGC 386 383 . . 383 387 + BlsI GCNGC 385 384 . . 383 387 + BisI GCNGC 384 385 . . 384 387 + AciI CCGC 384 386 . . 385 388 + FnuDII* CGCG 386 386 . . 397 402 + EcoP15I CAGCAG 427 429 . . 420 424 + Tsp4CI* ACNGT 422 421 . . 423 427 + MaeIII GTNAC 422 427 . . 428 433 + ApoI RAATTY 428 432 . . 429 432 + TspEI AATT 428 432 . . 455 459 + BccI CCATC 463 464 . . 456 461 - BtgZI GCGATG 441 445 . . 465 470 + AsuII TTCGAA 466 468 . . 466 469 + TaqI TCGA 466 468 . . 471 474 + SetI ASST 474 470 . . 480 483 + CviRI* TGCA 481 481 . . 481 486 + BtsI GCAGTG 488 486 . . 482 488 + TspRI CASTGNN 488 479 . . 484 489 + Hpy166II GTNNAC 486 486 . . 487 497 + Hin4I GAYNNNNNVTC 478 473 510 505 495 500 + Ksp632I* CTCTTC 501 504 . . 496 500 - MboII GAAGA 488 487 . . 501 504 + TspEI AATT 500 504 . . 517 520 + MseI TTAA 517 519 . . 528 533 + ApoI RAATTY 528 532 . . 529 532 + TspEI AATT 528 532 . . 538 541 + TspEI AATT 537 541 . . 547 551 + TfiI GAWTC 547 550 . . 547 551 + HinfI GANTC 547 550 . . 558 563 + SmlI CTYRAG 558 562 . . 563 567 + MaeIII GTNAC 562 567 . . 558 563 - Bce83I* CTTGAG 543 541 . . 570 574 + Tsp4CI* ACNGT 572 571 . . 573 577 + MaeIII GTNAC 572 577 . . 580 584 + BsrI ACTGG 585 583 . . 594 599 + EcoT22I ATGCAT 598 594 . . 595 598 + CviRI* TGCA 596 596 . . 604 609 + DsaI* CCRYGG 604 608 . . 604 609 + StyI CCWWGG 604 608 . . 604 609 + NcoI CCATGG 604 608 . . 604 609 + SecI* CCNNGG 604 608 . . 605 608 + FatI CATG 604 608 . . 605 608 + NlaIII CATG 608 604 . . 605 608 + CviAII CATG 605 607 . . 647 650 + CviRI* TGCA 648 648 . . 648 659 + AlfI GCANNNNNNTGC 637 635 671 669 649 658 + MslI CAYNNNNRTG 653 653 . . 656 661 + SduI GDGCHC 660 656 . . 656 661 + HgiAI* GWGCWC 660 656 . . 648 659 - AlfI GCANNNNNNTGC 671 669 637 635 662 672 + BsiYI* CCNNNNNNNGG 668 665 . . 662 672 + PflMI CCANNNNNTGG 668 665 . . 681 685 + Tsp4CI* ACNGT 683 682 . . 686 689 + AciI CCGC 686 688 . . 701 704 + TspEI AATT 700 704 . . 710 715 + ApoI RAATTY 710 714 . . 711 714 + TspEI AATT 710 714 . . 723 728 + NlaIV GGNNCC 725 725 . . 727 731 + BccI CCATC 735 736 . . 745 748 + TspEI AATT 744 748 . . 753 756 + SetI ASST 756 752 . . 753 756 + CviJI RGCY 754 754 . . 753 756 + AluI AGCT 754 754 . . 786 791 + Hpy178III* TCNNGA 787 789 . . 814 819 + ApoI RAATTY 814 818 . . 815 818 + TspEI AATT 814 818 . . 818 821 + MseI TTAA 818 820 . . 829 834 + BsgI GTGCAG 850 848 . . 830 833 + CviRI* TGCA 831 831 . . 836 841 + MmeI TCCRAC 861 859 . . 841 844 + FatI CATG 840 844 . . 841 844 + NlaIII CATG 844 840 . . 841 844 + CviAII CATG 841 843 . . 842 846 + TspDTI ATGAA 857 855 . . 844 849 + EcoRI GAATTC 844 848 . . 844 849 + ApoI RAATTY 844 848 . . 845 848 + TspEI AATT 844 848 . . 847 851 - TspDTI ATGAA 837 835 . . 868 871 + CviRI* TGCA 869 869 . . 869 874 + NspI RCATGY 873 869 . . 870 873 + FatI CATG 869 873 . . 870 873 + NlaIII CATG 873 869 . . 870 873 + CviAII CATG 870 872 . . 872 877 + TatI WGTACW 872 876 . . 873 876 + Csp6I GTAC 873 875 . . 873 876 + RsaI GTAC 874 874 . . 877 882 + Hpy178III* TCNNGA 878 880 . . 877 882 + BetI* WCCGGW 877 881 . . 877 882 + BspMII* TCCGGA 877 881 . . 878 881 + HpaII CCGG 878 880 . . 881 885 + TfiI GAWTC 881 884 . . 881 885 + HinfI GANTC 881 884 . . 886 889 + TspEI AATT 885 889 . . 893 897 + MaeIII GTNAC 892 897 . . 891 896 - TsoI TARCCA 881 879 . . 898 901 + TspEI AATT 897 901 . . 907 911 + TfiI GAWTC 907 910 . . 907 911 + HinfI GANTC 907 910 . . 919 923 - TspDTI ATGAA 909 907 . . 928 931 + DpnI GATC 929 929 . . 928 931 + BstKTI GATC 930 928 . . 928 931 + MboI GATC 927 931 . . 933 938 + Cac8I GCNNGC 935 935 . . 936 939 + SetI ASST 939 935 . . 936 939 + CviJI RGCY 937 937 . . 936 939 + AluI AGCT 937 937 . . 954 957 + SetI ASST 957 953 . . 957 962 + AhaIII* TTTAAA 959 959 . . 958 961 + MseI TTAA 958 960 . . 965 969 + TauI GCSGC 968 965 . . 965 969 + BlsI GCNGC 967 966 . . 965 969 + BisI GCNGC 966 967 . . 967 970 + CviJI RGCY 968 968 . . 965 968 - AciI CCGC 965 967 . . 985 989 + MboII GAAGA 997 996 . . 981 985 - Hin4II* CCTTC 975 974 . . 985 990 - Ksp632I* CTCTTC 980 983 . . 985 991 - SapI GCTCTTC 980 983 . . 988 998 - Hin4I GAYNNNNNVTC 1011 1006 979 974 1000 1004 + BmgT120I GGNCC 1001 1002 . . 1000 1004 + AvaII GGWCC 1000 1003 . . 1000 1004 + AsuI* GGNCC 1000 1003 . . 1002 1006 - TspGWI ACGGA 992 990 . . 1011 1016 + ApoI RAATTY 1011 1015 . . 1012 1015 + TspEI AATT 1011 1015 . . 1024 1028 + TfiI GAWTC 1024 1027 . . 1024 1028 + HinfI GANTC 1024 1027 . . 1033 1037 + PleI GAGTC 1041 1042 . . 1033 1037 + MlyI GAGTC 1042 1042 . . 1033 1037 + HinfI GANTC 1033 1036 . . 1036 1040 + Hpy188I TCNGA 1038 1037 . . 1035 1040 - MmeI TCCRAC 1016 1014 . . 1048 1051 + CviJI RGCY 1049 1049 . . 1048 1053 + NlaIV GGNNCC 1050 1050 . . 1046 1049 - MnlI CCTC 1039 1038 . . 1088 1093 + AsuII TTCGAA 1089 1091 . . 1089 1092 + TaqI TCGA 1089 1091 . . 1100 1104 + TspDTI ATGAA 1115 1113 . . 1103 1106 + TspEI AATT 1102 1106 . . 1118 1121 + MnlI CCTC 1128 1127 . . 1121 1126 + SmlI CTYRAG 1121 1125 . . 1122 1127 + Hpy178III* TCNNGA 1123 1125 . . 1121 1126 - Bce83I* CTTGAG 1106 1104 . . 1128 1132 + MaeIII GTNAC 1127 1132 . . 1134 1139 + ApoI RAATTY 1134 1138 . . 1135 1138 + TspEI AATT 1134 1138 . . 1141 1145 + MboII GAAGA 1153 1152 . . 1141 1146 + BbvII* GAAGAC 1148 1152 . . 1150 1160 + FalI AAGNNNNNCTT 1141 1136 1173 1168 1150 1160 - FalI AAGNNNNNCTT 1173 1168 1141 1136 1168 1173 + SspI AATATT 1170 1170 . . 1171 1176 + VspI ATTAAT 1172 1174 . . 1172 1175 + MseI TTAA 1172 1174 . . 1177 1181 + TspDTI ATGAA 1192 1190 . . 1186 1189 + AciI CCGC 1186 1188 . . 1199 1204 + Ksp632I* CTCTTC 1205 1208 . . 1200 1204 - MboII GAAGA 1192 1191 . . 1206 1211 + Hpy166II GTNNAC 1208 1208 . . 1206 1211 + AccI GTMKAC 1207 1209 . . 1203 1208 - TaqII GACCGA 1193 1191 . . 1225 1230 + Hpy166II GTNNAC 1227 1227 . . 1231 1239 + AlwNI CAGNNNCTG 1236 1233 . . 1230 1234 - BsrI ACTGG 1230 1228 . . 1235 1240 + Hpy178III* TCNNGA 1236 1238 . . 1242 1245 + FatI CATG 1241 1245 . . 1242 1245 + NlaIII CATG 1245 1241 . . 1242 1245 + CviAII CATG 1242 1244 . . 1243 1247 + TspDTI ATGAA 1258 1256 . . 1245 1249 + TfiI GAWTC 1245 1248 . . 1245 1249 + HinfI GANTC 1245 1248 . . 1265 1274 + XmnI GAANNNNTTC 1269 1269 . . 1265 1270 + ApoI RAATTY 1265 1269 . . 1266 1269 + TspEI AATT 1265 1269 . . 1270 1274 - MboII GAAGA 1262 1261 . . 1289 1293 + FokI GGATG 1302 1306 . . 1289 1293 + BseGI GGATG 1295 1293 . . 1293 1297 - BccI CCATC 1287 1288 . . 1304 1315 + CspCI CAANNNNNGTGG 1292 1290 1327 1325 1306 1311 + SspI AATATT 1308 1308 . . 1314 1319 + NlaIV GGNNCC 1316 1316 . . 1326 1330 + FokI GGATG 1339 1343 . . 1326 1330 + BseGI GGATG 1332 1330 . . 1330 1333 + DpnI GATC 1331 1331 . . 1330 1333 + BstKTI GATC 1332 1330 . . 1330 1333 + MboI GATC 1329 1333 . . 1333 1337 + BssKI CCNGG 1332 1337 . . 1333 1336 + HpaII CCGG 1333 1335 . . 1333 1337 + ScrFI CCNGG 1334 1335 . . 1333 1337 + CauII* CCSGG 1334 1335 . . 1330 1334 - BinI* GGATC 1324 1325 . . 1336 1341 + NlaIV GGNNCC 1338 1338 . . 1360 1363 + MseI TTAA 1360 1362 . . 1366 1369 + AciI CCGC 1366 1368 . . 1368 1378 + MwoI GCNNNNNNNGC 1374 1371 . . 1370 1373 + TspEI AATT 1369 1373 . . 1375 1378 + AciI CCGC 1375 1377 . . 1381 1385 + TspDTI ATGAA 1396 1394 . . 1384 1389 + SspI AATATT 1386 1386 . . 1389 1393 - TspGWI ACGGA 1379 1377 . . 1396 1399 + TspEI AATT 1395 1399 . . 1400 1403 + MaeI CTAG 1400 1402 . . 1405 1408 + TspEI AATT 1404 1408 . . 1411 1416 + NlaIV GGNNCC 1413 1413 . . 1411 1416 + HgiCI* GGYRCC 1411 1415 . . 1417 1421 + Hin4II* CCTTC 1427 1426 . . 1456 1460 + BceAI ACGGC 1472 1474 . . 1458 1461 + CviJI RGCY 1459 1459 . . 1474 1478 + BccI CCATC 1482 1483 . . 1477 1480 + TaqI TCGA 1477 1479 . . 1493 1497 + BlsI GCNGC 1495 1494 . . 1493 1497 + BisI GCNGC 1494 1495 . . 1493 1497 + BbvI GCAGC 1505 1509 . . 1493 1497 + TseI GCWGC 1493 1496 . . 1495 1498 + CviJI RGCY 1496 1496 . . 1504 1513 + MslI CAYNNNNRTG 1508 1508 . . 1513 1518 + Hpy166II GTNNAC 1515 1515 . . 1513 1518 + HpaI GTTAAC 1515 1515 . . 1513 1518 + HindII GTYRAC 1515 1515 . . 1514 1517 + MseI TTAA 1514 1516 . . 1529 1532 + MseI TTAA 1529 1531 . . 1534 1537 + SetI ASST 1537 1533 . . 1535 1538 + MnlI CCTC 1545 1544 . . 1538 1543 + PstI CTGCAG 1542 1538 . . 1538 1543 + SfeI* CTRYAG 1538 1542 . . 1539 1542 + CviRI* TGCA 1540 1540 . . 1540 1544 + BlsI GCNGC 1542 1541 . . 1540 1544 + BisI GCNGC 1541 1542 . . 1540 1544 + BbvI GCAGC 1552 1556 . . 1540 1544 + TseI GCWGC 1540 1543 . . 1542 1545 + CviJI RGCY 1543 1543 . . 1543 1547 + TauI GCSGC 1546 1543 . . 1543 1547 + BlsI GCNGC 1545 1544 . . 1543 1547 + BisI GCNGC 1544 1545 . . 1544 1547 + AciI CCGC 1544 1546 . . 1546 1550 + SfaNI GCATC 1555 1559 . . 1571 1574 + Csp6I GTAC 1571 1573 . . 1571 1574 + RsaI GTAC 1572 1572 . . 1582 1587 + HaeII RGCGCY 1586 1582 . . 1582 1587 + Eco47III AGCGCT 1584 1584 . . 1583 1586 + Hin6I GCGC 1583 1585 . . 1583 1586 + GlaI GCGC 1584 1584 . . 1583 1586 + HhaI GCGC 1585 1583 . . 1609 1614 + AclI AACGTT 1610 1612 . . 1610 1613 + TaiI ACGT 1613 1609 . . 1610 1613 + SetI ASST 1613 1609 . . 1610 1613 + MaeII ACGT 1610 1612 . . 1617 1623 + TspRI CASTGNN 1623 1614 . . 1618 1622 + Tsp4CI* ACNGT 1620 1619 . . 1624 1628 + Hin4II* CCTTC 1634 1633 . . 1627 1631 - MboII GAAGA 1619 1618 . . 1636 1641 + EcoT22I ATGCAT 1640 1636 . . 1637 1640 + CviRI* TGCA 1638 1638 . . 1635 1639 - SfaNI GCATC 1625 1629 . . 1638 1643 - BsmI GAATGC 1638 1636 . . 1644 1649 + Hpy166II GTNNAC 1646 1646 . . 1650 1655 + AhaIII* TTTAAA 1652 1652 . . 1651 1654 + MseI TTAA 1651 1653 . . 1657 1661 + BslFI GGGAC 1671 1675 . . 1659 1669 + Hin4I GAYNNNNNVTC 1650 1645 1682 1677 1659 1669 + Eam1105I GACNNNNNGTC 1664 1663 . . 1665 1668 + SetI ASST 1668 1664 . . 1661 1666 - Eco57MI CTGRAG 1646 1644 . . 1661 1666 - Eco57I CTGAAG 1646 1644 . . 1659 1669 - Hin4I GAYNNNNNVTC 1682 1677 1650 1645 1669 1672 + MaeI CTAG 1669 1671 . . 1673 1677 + TauI GCSGC 1676 1673 . . 1673 1677 + BlsI GCNGC 1675 1674 . . 1673 1677 + BisI GCNGC 1674 1675 . . 1673 1676 - AciI CCGC 1673 1675 . . 1698 1701 + SetI ASST 1701 1697 . . 1698 1701 + CviJI RGCY 1699 1699 . . 1698 1701 + AluI AGCT 1699 1699 . . 1702 1705 + MseI TTAA 1702 1704 . . 1711 1721 + MwoI GCNNNNNNNGC 1717 1714 . . 1715 1721 + TspRI CASTGNN 1721 1712 . . 1716 1720 + BsrI ACTGG 1721 1719 . . 1719 1722 + CviJI RGCY 1720 1720 . . 1720 1730 + MwoI GCNNNNNNNGC 1726 1723 . . 1718 1723 - TsoI TARCCA 1708 1706 . . 1726 1731 + HaeII RGCGCY 1730 1726 . . 1726 1731 + Eco47III AGCGCT 1728 1728 . . 1727 1730 + Hin6I GCGC 1727 1729 . . 1727 1730 + GlaI GCGC 1728 1728 . . 1727 1730 + HhaI GCGC 1729 1727 . . 1737 1741 + Tsp45I GTSAC 1736 1741 . . 1737 1741 + MaeIII GTNAC 1736 1741 . . 1735 1739 - HgaI GACGC 1724 1729 . . 1738 1742 - HphI GGTGA 1730 1729 . . 1750 1753 + TaiI ACGT 1753 1749 . . 1750 1753 + SetI ASST 1753 1749 . . 1750 1753 + MaeII ACGT 1750 1752 . . 1752 1756 + MaeIII GTNAC 1751 1756 . . 1756 1759 + AciI CCGC 1756 1758 . . 1758 1762 + TauI GCSGC 1761 1758 . . 1758 1762 + BlsI GCNGC 1760 1759 . . 1758 1762 + BisI GCNGC 1759 1760 . . 1759 1764 + NspBII* CMGCKG 1761 1761 . . 1759 1762 + AciI CCGC 1759 1761 . . 1767 1771 + Tsp45I GTSAC 1766 1771 . . 1767 1771 + MaeIII GTNAC 1766 1771 . . 1768 1772 - HphI GGTGA 1760 1759 . . 1774 1778 + TspDTI ATGAA 1789 1787 . . 1776 1780 + TfiI GAWTC 1776 1779 . . 1776 1780 + HinfI GANTC 1776 1779 . . 1779 1783 + Hpy188I TCNGA 1781 1780 . . 1784 1788 + BceAI ACGGC 1800 1802 . . 1785 1790 + CfrI YGGCCR 1785 1789 . . 1786 1789 + CviJI RGCY 1787 1787 . . 1786 1789 + HaeIII GGCC 1787 1787 . . 1792 1795 + SetI ASST 1795 1791 . . 1793 1796 + MnlI CCTC 1803 1802 . . 1813 1817 + TspDTI ATGAA 1828 1826 . . 1818 1829 + CspCI CAANNNNNGTGG 1806 1804 1841 1839 1823 1827 + Tsp4CI* ACNGT 1825 1824 . . 1824 1830 + TspRI CASTGNN 1830 1821 . . 1838 1848 + MwoI GCNNNNNNNGC 1844 1841 . . 1841 1845 + Tsp4CI* ACNGT 1843 1842 . . 1852 1855 + SetI ASST 1855 1851 . . 1852 1855 + CviJI RGCY 1853 1853 . . 1852 1855 + AluI AGCT 1853 1853 . . 1862 1866 + Tsp4CI* ACNGT 1864 1863 . . 1867 1871 + Tsp45I GTSAC 1866 1871 . . 1867 1871 + MaeIII GTNAC 1866 1871 . . 1873 1877 + BceAI ACGGC 1889 1891 . . 1875 1878 + CviJI RGCY 1876 1876 . . 1876 1880 + BlsI GCNGC 1878 1877 . . 1876 1880 + BisI GCNGC 1877 1878 . . 1876 1880 + TseI GCWGC 1876 1879 . . 1879 1883 + BlsI GCNGC 1881 1880 . . 1879 1883 + BisI GCNGC 1880 1881 . . 1879 1883 + TseI GCWGC 1879 1882 . . 1876 1880 - BbvI GCAGC 1863 1867 . . 1877 1882 - EcoP15I CAGCAG 1849 1851 . . 1879 1883 - BbvI GCAGC 1866 1870 . . 1896 1901 + HaeII RGCGCY 1900 1896 . . 1897 1900 + Hin6I GCGC 1897 1899 . . 1897 1900 + GlaI GCGC 1898 1898 . . 1897 1900 + HhaI GCGC 1899 1897 . . 1900 1903 + MnlI CCTC 1910 1909 . . 1903 1914 + TstI CACNNNNNNTCC 1894 1889 1926 1921 1920 1924 - BslFI GGGAC 1905 1909 . . 1929 1934 + MmeI TCCRAC 1954 1952 . . 1937 1941 + Tsp4CI* ACNGT 1939 1938 . . 1941 1945 + Hpy188I TCNGA 1943 1942 . . 1944 1948 + TfiI GAWTC 1944 1947 . . 1944 1948 + HinfI GANTC 1944 1947 . . 1984 1987 + MseI TTAA 1984 1986 . . 1986 1991 + ApoI RAATTY 1986 1990 . . 1987 1990 + TspEI AATT 1986 1990 . . 1992 1995 + SetI ASST 1995 1991 . . 1998 2001 + CviRI* TGCA 1999 1999 . . 2010 2013 + SetI ASST 2013 2009 . . 2011 2015 + Hin4II* CCTTC 2021 2020 . . 2008 2012 - HphI GGTGA 2000 1999 . . 2025 2029 - FokI GGATG 2011 2015 . . 2025 2029 - BseGI GGATG 2024 2022 . . 2035 2040 + SmlI CTYRAG 2035 2039 . . 2036 2041 + Hpy178III* TCNNGA 2037 2039 . . 2035 2040 - Bce83I* CTTGAG 2020 2018 . . 2040 2043 - MnlI CCTC 2033 2032 . . 2053 2057 + MaeIII GTNAC 2052 2057 . . 2051 2056 - TsoI TARCCA 2041 2039 . . 2059 2062 + MseI TTAA 2059 2061 . . 2062 2067 + SspI AATATT 2064 2064 . . 2068 2073 + Hpy178III* TCNNGA 2069 2071 . . 2070 2080 + FalI AAGNNNNNCTT 2061 2056 2093 2088 2075 2080 + HindIII AAGCTT 2075 2079 . . 2076 2079 + SetI ASST 2079 2075 . . 2076 2079 + CviJI RGCY 2077 2077 . . 2076 2079 + AluI AGCT 2077 2077 . . 2070 2080 - FalI AAGNNNNNCTT 2093 2088 2061 2056 2085 2090 + SduI GDGCHC 2089 2085 . . 2085 2090 + HgiJII* GRGCYC 2089 2085 . . 2086 2089 + CviJI RGCY 2087 2087 . . 2095 2099 - MboII GAAGA 2087 2086 . . 2110 2120 + Hin4I GAYNNNNNVTC 2101 2096 2133 2128 2112 2117 + SfeI* CTRYAG 2112 2116 . . 2116 2121 + XhoII RGATCY 2116 2120 . . 2116 2121 + BglII AGATCT 2116 2120 . . 2117 2120 + DpnI GATC 2118 2118 . . 2117 2120 + BstKTI GATC 2119 2117 . . 2117 2120 + MboI GATC 2116 2120 . . 2110 2120 - Hin4I GAYNNNNNVTC 2133 2128 2101 2096 2121 2125 - TspGWI ACGGA 2111 2109 . . 2126 2131 - TaqII CACCCA 2116 2114 . . 2145 2148 + FatI CATG 2144 2148 . . 2145 2148 + NlaIII CATG 2148 2144 . . 2145 2148 + CviAII CATG 2145 2147 . . 2152 2156 - MboII GAAGA 2144 2143 . . 2177 2181 + Tsp4CI* ACNGT 2179 2178 . . 2180 2183 + Csp6I GTAC 2180 2182 . . 2180 2183 + RsaI GTAC 2181 2181 . . 2182 2186 + BsrI ACTGG 2187 2185 . . 2186 2191 + EcoRI GAATTC 2186 2190 . . 2186 2191 + ApoI RAATTY 2186 2190 . . 2187 2190 + TspEI AATT 2186 2190 . . 2206 2211 + SspI AATATT 2208 2208 . . 2209 2214 + VspI ATTAAT 2210 2212 . . 2210 2213 + MseI TTAA 2210 2212 . . 2217 2221 + DdeI CTNAG 2217 2220 . . 2223 2226 + CviRI* TGCA 2224 2224 . . 2221 2225 - SfaNI GCATC 2211 2215 . . 2226 2229 + SetI ASST 2229 2225 . . 2227 2231 + BmgT120I GGNCC 2228 2229 . . 2227 2231 + AvaII GGWCC 2227 2230 . . 2227 2231 + AsuI* GGNCC 2227 2230 . . 2224 2229 - BspMI ACCTGC 2215 2219 . . 2230 2234 + BccI CCATC 2238 2239 . . 2242 2245 + MnlI CCTC 2252 2251 . . 2240 2245 - BseRI GAGGAG 2231 2229 . . 2270 2274 + Tsp4CI* ACNGT 2272 2271 . . 2289 2294 + Hpy178III* TCNNGA 2290 2292 . . 2294 2298 + Tsp4CI* ACNGT 2296 2295 . . 2302 2305 + TspEI AATT 2301 2305 . . 2312 2316 + Tsp4CI* ACNGT 2314 2313 . . 2324 2328 + MaeIII GTNAC 2323 2328 . . 2335 2339 + TfiI GAWTC 2335 2338 . . 2335 2339 + HinfI GANTC 2335 2338 . . 2344 2348 + BccI CCATC 2352 2353 . . 2351 2356 + Hpy178III* TCNNGA 2352 2354 . . 2377 2386 + BsaBI GATNNNNATC 2381 2381 . . 2387 2390 + Csp6I GTAC 2387 2389 . . 2387 2390 + RsaI GTAC 2388 2388 . . 2392 2396 - MboII GAAGA 2384 2383 . . 2398 2402 + TspDTI ATGAA 2413 2411 . . 2408 2412 + BceAI ACGGC 2424 2426 . . 2416 2419 + TspEI AATT 2415 2419 . . 2425 2428 + Hin6I GCGC 2425 2427 . . 2425 2428 + GlaI GCGC 2426 2426 . . 2425 2428 + HhaI GCGC 2427 2425 . . 2427 2432 - BsmI GAATGC 2427 2425 . . 2431 2435 - MboII GAAGA 2423 2422 . . 2435 2439 + DdeI CTNAG 2435 2438 . . 2443 2447 + HphI GGTGA 2455 2454 . . 2453 2456 + SetI ASST 2456 2452 . . 2452 2455 - MnlI CCTC 2445 2444 . . 2464 2468 + Hpy188I TCNGA 2466 2465 . . 2467 2470 + DpnI GATC 2468 2468 . . 2467 2470 + BstKTI GATC 2469 2467 . . 2467 2470 + MboI GATC 2466 2470 . . 2470 2473 + FatI CATG 2469 2473 . . 2470 2473 + NlaIII CATG 2473 2469 . . 2470 2473 + CviAII CATG 2470 2472 . . 2490 2495 + Hpy178III* TCNNGA 2491 2493 . . 2505 2509 + Tsp4CI* ACNGT 2507 2506 . . 2513 2516 + MnlI CCTC 2523 2522 . . 2518 2524 + BstEII GGTNACC 2518 2523 . . 2519 2523 + Tsp45I GTSAC 2518 2523 . . 2519 2523 + MaeIII GTNAC 2518 2523 . . 2523 2527 + BccI CCATC 2531 2532 . . 2520 2524 - HphI GGTGA 2512 2511 . . 2551 2560 + MslI CAYNNNNRTG 2555 2555 . . 2549 2553 - TspDTI ATGAA 2539 2537 . . 2554 2559 + BetI* WCCGGW 2554 2558 . . 2555 2558 + HpaII CCGG 2555 2557 . . 2557 2561 + HphI GGTGA 2569 2568 . . 2576 2586 + MwoI GCNNNNNNNGC 2582 2579 . . 2578 2588 + BsiYI* CCNNNNNNNGG 2584 2581 . . 2582 2586 - SfaNI GCATC 2572 2576 . . 2585 2588 - AciI CCGC 2585 2587 . . 2594 2598 + TfiI GAWTC 2594 2597 . . 2594 2598 + HinfI GANTC 2594 2597 . . 2597 2602 + Hpy178III* TCNNGA 2598 2600 . . 2603 2607 + MboII GAAGA 2615 2614 . . 2603 2608 - Ksp632I* CTCTTC 2598 2601 . . 2606 2610 - BsmAI GTCTC 2600 2604 . . 2611 2614 + FatI CATG 2610 2614 . . 2611 2614 + NlaIII CATG 2614 2610 . . 2611 2614 + CviAII CATG 2611 2613 . . 2606 2611 - Eco31I GGTCTC 2600 2604 . . 2606 2617 - PpiI GAACNNNNNCTC 2629 2624 2597 2592 2618 2621 + CviRI* TGCA 2619 2619 . . 2624 2631 + SwaI ATTTAAAT 2627 2627 . . 2625 2630 + AhaIII* TTTAAA 2627 2627 . . 2626 2629 + MseI TTAA 2626 2628 . . 2636 2641 + ApoI RAATTY 2636 2640 . . 2637 2640 + TspEI AATT 2636 2640 . . 2642 2645 + FatI CATG 2641 2645 . . 2642 2645 + NlaIII CATG 2645 2641 . . 2642 2645 + CviAII CATG 2642 2644 . . 2644 2647 + CviRI* TGCA 2645 2645 . . 2648 2653 + SmlI CTYRAG 2648 2652 . . 2649 2654 + Hpy178III* TCNNGA 2650 2652 . . 2648 2653 - Bce83I* CTTGAG 2633 2631 . . 2669 2672 + TspEI AATT 2668 2672 . . 2673 2676 + SetI ASST 2676 2672 . . 2673 2676 + CviJI RGCY 2674 2674 . . 2673 2676 + AluI AGCT 2674 2674 . . 2677 2681 + TfiI GAWTC 2677 2680 . . 2677 2681 + HinfI GANTC 2677 2680 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 1207 1478 # # AciI: # [CCGC] # 3 9 83 100 129 169 # 178 180 206 221 279 302 # 826 # # AclI: # [AACGTT] # 1075 1610 # # AhaIII*: # [TTTAAA] # 58 183 188 588 693 975 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 637 2014 # # AluI: # [AGCT] # 11 154 156 183 224 597 # 598 762 # # AlwNI: # [CAGNNNCTG] # 1236 1449 # # ApoI: # [RAATTY] # 30 31 49 69 100 104 # 123 131 167 182 200 328 # 450 721 # # AsuI*: # [GGNCC] # 458 1000 1227 # # AsuII: # [TTCGAA] # 466 623 1596 # # AvaII: # [GGWCC] # 458 1000 1227 # # BbvI: # [GCAGC] # 3 47 311 819 1505 # # BbvII*: # [GAAGAC] # 1148 1537 # # BccI: # [CCATC] # 22 29 114 154 176 179 # 195 236 272 552 756 # # Bce83I*: # [CTTGAG] # 52 543 563 613 914 # # BceAI: # [ACGGC] # 33 89 261 328 535 1439 # # BetI*: # [WCCGGW] # 36 131 841 1677 # # BglII: # [AGATCT] # 569 2116 # # BinI*: # [GGATC] # 1324 1361 # # BisI: # [GCNGC] # 3 3 47 85 118 130 # 384 528 582 805 # # BlsI: # [GCNGC] # 3 3 47 85 118 130 # 385 528 582 804 # # BmgT120I: # [GGNCC] # 457 1001 1227 # # BsaBI: # [GATNNNNATC] # 304 2381 # # BseGI: # [GGATG] # 37 661 692 1295 # # BseRI: # [GAGGAG] # 454 2231 # # BsgI: # [GTGCAG] # 850 1835 # # BsiYI*: # [CCNNNNNNNGG] # 87 101 146 435 1916 # # BslFI: # [GGGAC] # 234 250 780 1421 # # BsmAI: # [GTCTC] # 85 2600 # # BsmI: # [GAATGC] # 258 789 1638 # # BspMI: # [ACCTGC] # 470 2215 # # BspMII*: # [TCCGGA] # 877 1808 # # BsrI: # [ACTGG] # 13 466 491 498 572 645 # # BssKI: # [CCNGG] # 1332 1353 # # BstEII: # [GGTNACC] # 167 2518 # # BstKTI: # [GATC] # 216 350 402 787 930 # # BtgZI: # [GCGATG] # 195 246 2244 # # BtsI: # [GCAGTG] # 488 2197 # # Cac8I: # [GCNNGC] # 935 1750 # # CauII*: # [CCSGG] # 1334 1351 # # CfrI: # [YGGCCR] # 317 900 1468 # # Csp6I: # [GTAC] # 207 291 298 582 609 698 # # CspCI: # [CAANNNNNGTGG] # 35 35 479 844 1292 # # CviAII: # [CATG] # 29 31 43 141 236 244 # 325 361 372 903 # # CviJI: # [RGCY] # 6 10 11 11 12 21 # 23 31 36 37 47 59 # 66 67 81 93 102 156 # 183 201 410 435 587 # # CviRI*: # [TGCA] # 26 38 40 52 67 98 # 115 183 225 361 395 414 # 671 # # DdeI: # [CTNAG] # 218 250 2217 # # DpnI: # [GATC] # 217 350 402 787 929 # # DsaI*: # [CCRYGG] # 604 2081 # # Eam1105I: # [GACNNNNNGTC] # 285 1021 1379 # # Eco31I: # [GGTCTC] # 85 2600 # # Eco47III: # [AGCGCT] # 144 957 1584 # # Eco57I: # [CTGAAG] # 1039 1646 # # Eco57MI: # [CTGRAG] # 172 1039 1474 # # EcoP15I: # [CAGCAG] # 427 836 1422 # # EcoRI: # [GAATTC] # 499 844 1342 # # EcoT22I: # [ATGCAT] # 598 1042 1045 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 592 888 1141 # # FatI: # [CATG] # 29 31 44 141 236 243 # 325 361 372 903 # # FnuDII*: # [CGCG] # 386 2299 # # FokI: # [GGATG] # 37 672 674 1302 # # GlaI: # [GCGC] # 144 170 259 528 1584 # # GsuI: # [CTGGAG] # 172 2513 # # HaeII: # [RGCGCY] # 144 170 785 1586 # # HaeIII: # [GGCC] # 319 898 1468 # # HgaI: # [GACGC] # 961 1724 # # HgiAI*: # [GWGCWC] # 660 2025 # # HgiCI*: # [GGYRCC] # 1274 1411 # # HgiJII*: # [GRGCYC] # 596 2089 # # HhaI: # [GCGC] # 144 170 258 528 1585 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 32 32 32 32 32 175 # 271 419 469 552 639 # # Hin4II*: # [CCTTC] # 157 207 387 452 664 818 # # Hin6I: # [GCGC] # 144 170 260 528 1583 # # HindII: # [GTYRAC] # 1170 1515 # # HindIII: # [AAGCTT] # 610 2075 # # HinfI: # [GANTC] # 8 8 9 26 83 117 # 118 168 205 212 216 259 # 334 391 531 # # HpaI: # [GTTAAC] # 1170 1515 # # HpaII: # [CCGG] # 37 130 455 841 1222 # # HphI: # [GGTGA] # 30 57 57 116 161 200 # 240 455 1369 # # Hpy166II: # [GTNNAC] # 19 131 288 486 722 1039 # # Hpy178III*: # [TCNNGA] # 7 32 35 52 62 91 # 107 113 139 221 245 328 # 452 801 # # Hpy188I: # [TCNGA] # 106 162 219 523 743 932 # # Ksp632I*: # [CTCTTC] # 87 225 479 501 1393 # # MaeI: # [CTAG] # 126 202 269 1016 1072 # # MaeII: # [ACGT] # 140 935 1610 # # MaeIII: # [GTNAC] # 10 15 15 100 140 167 # 186 195 235 271 320 422 # 609 # # MboI: # [GATC] # 219 350 402 787 927 # # MboII: # [GAAGA] # 39 39 57 70 70 156 # 167 192 240 321 357 468 # 509 # # MlyI: # [GAGTC] # 126 199 717 1643 # # MmeI: # [TCCRAC] # 155 731 861 938 # # MnlI: # [CCTC] # 12 36 78 89 107 123 # 134 162 193 219 258 417 # 857 # # MseI: # [TTAA] # 14 15 44 51 58 59 # 75 111 122 140 143 147 # 151 154 188 214 282 301 # 416 # # MslI: # [CAYNNNNRTG] # 130 653 855 1047 # # MwoI: # [GCNNNNNNNGC] # 9 103 118 343 738 1374 # # NcoI: # [CCATGG] # 604 2081 # # NlaIII: # [CATG] # 29 31 40 141 236 247 # 325 361 372 903 # # NlaIV: # [GGNNCC] # 22 75 266 325 725 1272 # # NspBII*: # [CMGCKG] # 924 1761 # # NspI: # [RCATGY] # 873 1812 # # PflMI: # [CCANNNNNTGG] # 87 146 435 2017 # # PleI: # [GAGTC] # 126 199 716 1644 # # PpiI: # [GAACNNNNNCTC] # 32 56 2597 # # PstI: # [CTGCAG] # 1143 1542 # # RsaI: # [GTAC] # 207 292 297 582 609 698 # # SapI: # [GCTCTTC] # 980 1705 # # ScrFI: # [CCNGG] # 1334 1351 # # SduI: # [GDGCHC] # 596 660 1429 # # SecI*: # [CCNNGG] # 604 2081 # # SetI: # [ASST] # 9 16 18 18 23 33 # 42 52 55 60 66 76 # 123 135 140 150 177 183 # 220 227 282 580 # # SfaNI: # [GCATC] # 70 113 272 361 586 1283 # # SfeI*: # [CTRYAG] # 573 574 1538 # # SmlI: # [CTYRAG] # 37 558 563 613 914 # # SspI: # [AATATT] # 78 79 138 144 477 678 # 1091 # # StyI: # [CCWWGG] # 604 2081 # # SwaI: # [ATTTAAAT] # 58 2627 # # TaiI: # [ACGT] # 140 932 1613 # # TaqI: # [TCGA] # 70 130 266 388 623 1208 # # TaqII: # [GACCGA] # 569 923 1193 # # TatI: # [WGTACW] # 872 1813 # # TauI: # [GCSGC] # 85 130 386 578 582 924 # # TfiI: # [GAWTC] # 8 26 83 117 168 213 # 221 259 334 334 391 531 # # TseI: # [GCWGC] # 3 47 336 806 1493 # # TsoI: # [TARCCA] # 333 644 827 881 # # Tsp45I: # [GTSAC] # 30 100 167 652 1736 # # Tsp4CI*: # [ACNGT] # 18 18 21 24 52 75 # 93 111 117 150 178 193 # 205 240 253 937 # # TspDTI: # [ATGAA] # 20 32 39 52 66 72 # 77 126 138 146 156 206 # 393 577 585 # # TspEI: # [AATT] # 8 9 9 10 12 17 # 23 26 28 30 32 32 # 34 41 50 69 70 72 # 84 91 104 114 114 115 # 131 163 194 200 221 582 # # TspGWI: # [ACGGA] # 387 574 732 992 # # TspRI: # [CASTGNN] # 98 109 178 310 855 1135 # # TstI: # [CACNNNNNNTCC] # 32 759 1894 # # VspI: # [ATTAAT] # 475 1038 1172 # # XbaI: # [TCTAGA] # 327 2358 # # XcmI: # [CCANNNNNNNNNTGG] # 147 2538 # # XhoII: # [RGATCY] # 569 2116 # # XmnI: # [GAANNNNTTC] # 1269 1416 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2685 # Reported_sequences: 1 # Reported_hitcount: 575 #---------------------------------------