Restriction Fragments for PPH3/YDR075W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

PPH3/YDR075W on chromosome IV from coordinates 597156 to 598082.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.14189.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 9 13 + DdeI CTNAG 9 12 . . 25 29 + SfaNI GCATC 34 38 . . 29 35 + TspRI CASTGNN 35 26 . . 31 35 + DdeI CTNAG 31 34 . . 31 35 - BspCNI CTCAG 23 21 . . 31 35 - BseMII CTCAG 22 20 . . 38 42 + TspGWI ACGGA 53 51 . . 35 39 - BsmAI GTCTC 29 33 . . 35 40 - Esp3I CGTCTC 29 33 . . 51 56 + Hpy178III* TCNNGA 52 54 . . 53 58 + Eco57MI CTGRAG 74 72 . . 53 58 + Eco57I CTGAAG 74 72 . . 55 59 + MboII GAAGA 67 66 . . 61 65 + Tsp4CI* ACNGT 63 62 . . 62 72 + BsiYI* CCNNNNNNNGG 68 65 . . 71 74 + CviJI RGCY 72 72 . . 78 83 + AhaIII* TTTAAA 80 80 . . 79 82 + MseI TTAA 79 81 . . 81 86 + ApoI RAATTY 81 85 . . 82 85 + TspEI AATT 81 85 . . 96 101 + VspI ATTAAT 97 99 . . 97 100 + MseI TTAA 97 99 . . 100 104 + TspDTI ATGAA 115 113 . . 104 108 + TspDTI ATGAA 119 117 . . 106 110 - Hin4II* CCTTC 100 99 . . 110 115 + BsrDI GCAATG 117 115 . . 115 119 + MaeIII GTNAC 114 119 . . 125 128 + TaqI TCGA 125 127 . . 132 137 + Cfr10I RCCGGY 132 136 . . 132 137 + BetI* WCCGGW 132 136 . . 132 137 + AgeI ACCGGT 132 136 . . 133 136 + HpaII CCGG 133 135 . . 136 140 + MaeIII GTNAC 135 140 . . 127 137 - BaeI ACNNNNGTAYC 152 147 119 114 148 152 + HphI GGTGA 160 159 . . 146 149 - AciI CCGC 146 148 . . 157 160 + FatI CATG 156 160 . . 157 160 + NlaIII CATG 160 156 . . 157 160 + CviAII CATG 157 159 . . 159 164 + BalI TGGCCA 161 161 . . 159 164 + CfrI YGGCCR 159 163 . . 160 163 + CviJI RGCY 161 161 . . 160 163 + HaeIII GGCC 161 161 . . 164 167 + TspEI AATT 163 167 . . 172 175 + DpnI GATC 173 173 . . 172 175 + BstKTI GATC 174 172 . . 172 175 + MboI GATC 171 175 . . 183 189 + SapI GCTCTTC 190 193 . . 184 189 + Ksp632I* CTCTTC 190 193 . . 187 192 + AsuII TTCGAA 188 190 . . 188 191 + TaqI TCGA 188 190 . . 185 189 - MboII GAAGA 177 176 . . 217 220 + SetI ASST 220 216 . . 287 291 + MaeIII GTNAC 286 291 . . 293 296 + TspEI AATT 292 296 . . 295 298 + MseI TTAA 295 297 . . 299 304 + EcoRV GATATC 301 301 . . 303 308 + Hpy178III* TCNNGA 304 306 . . 319 322 + MseI TTAA 319 321 . . 321 324 + TspEI AATT 320 324 . . 326 329 - MnlI CCTC 319 318 . . 333 338 + Hpy178III* TCNNGA 334 336 . . 341 345 + BssKI CCNGG 340 345 . . 341 345 + ScrFI CCNGG 342 343 . . 341 345 + CauII* CCSGG 342 343 . . 342 345 + HpaII CCGG 342 344 . . 348 351 + TspEI AATT 347 351 . . 358 363 + Hpy166II GTNNAC 360 360 . . 358 363 + BssNAI GTATAC 360 360 . . 358 363 + AccI GTMKAC 359 361 . . 362 366 + TspGWI ACGGA 377 375 . . 374 378 + TspDTI ATGAA 389 387 . . 401 405 + MaeIII GTNAC 400 405 . . 404 407 + TaiI ACGT 407 403 . . 404 407 + SetI ASST 407 403 . . 404 407 + MaeII ACGT 404 406 . . 412 415 + SetI ASST 415 411 . . 411 414 - MnlI CCTC 404 403 . . 414 417 + Csp6I GTAC 414 416 . . 414 417 + RsaI GTAC 415 415 . . 417 422 - EcoP15I CAGCAG 389 391 . . 413 423 - BaeI ACNNNNGTAYC 438 433 405 400 433 443 + Hin4I GAYNNNNNVTC 424 419 456 451 433 443 - Hin4I GAYNNNNNVTC 456 451 424 419 481 484 + FatI CATG 480 484 . . 481 484 + NlaIII CATG 484 480 . . 481 484 + CviAII CATG 481 483 . . 479 483 - TspDTI ATGAA 469 467 . . 495 500 + Hpy178III* TCNNGA 496 498 . . 495 500 + BetI* WCCGGW 495 499 . . 495 500 + BspMII* TCCGGA 495 499 . . 496 499 + HpaII CCGG 496 498 . . 506 511 + DsaI* CCRYGG 506 510 . . 506 511 + SecI* CCNNGG 506 510 . . 508 512 + Tsp4CI* ACNGT 510 509 . . 515 519 + TspDTI ATGAA 530 528 . . 550 560 + BsiYI* CCNNNNNNNGG 556 553 . . 553 562 + MslI CAYNNNNRTG 557 557 . . 553 556 + FatI CATG 552 556 . . 553 556 + NlaIII CATG 556 552 . . 553 556 + CviAII CATG 553 555 . . 554 558 + TspDTI ATGAA 569 567 . . 558 561 + SetI ASST 561 557 . . 556 560 - Hin4II* CCTTC 550 549 . . 569 573 + Tsp45I GTSAC 568 573 . . 569 573 + MaeIII GTNAC 568 573 . . 592 596 + MboII GAAGA 604 603 . . 592 597 + BbvII* GAAGAC 599 603 . . 596 599 + TaiI ACGT 599 595 . . 596 599 + SetI ASST 599 595 . . 596 599 + MaeII ACGT 596 598 . . 598 603 + Hpy166II GTNNAC 600 600 . . 598 603 + HindII GTYRAC 600 600 . . 601 611 + Hin4I GAYNNNNNVTC 592 587 624 619 601 611 + Eam1105I GACNNNNNGTC 606 605 . . 605 608 + FatI CATG 604 608 . . 605 608 + NlaIII CATG 608 604 . . 605 608 + CviAII CATG 605 607 . . 601 611 - Hin4I GAYNNNNNVTC 624 619 592 587 616 620 - HphI GGTGA 608 607 . . 624 627 + SetI ASST 627 623 . . 623 626 - MnlI CCTC 616 615 . . 657 662 + BclI TGATCA 657 661 . . 658 661 + DpnI GATC 659 659 . . 658 661 + BstKTI GATC 660 658 . . 658 661 + MboI GATC 657 661 . . 662 665 + TspEI AATT 661 665 . . 666 670 + DdeI CTNAG 666 669 . . 679 684 + AclI AACGTT 680 682 . . 680 683 + TaiI ACGT 683 679 . . 680 683 + SetI ASST 683 679 . . 680 683 + MaeII ACGT 680 682 . . 688 691 + MseI TTAA 688 690 . . 690 693 + TspEI AATT 689 693 . . 695 698 + MaeI CTAG 695 697 . . 698 703 + SacI GAGCTC 702 698 . . 698 703 + Ecl136II GAGCTC 700 700 . . 698 703 + SduI GDGCHC 702 698 . . 698 703 + HgiJII* GRGCYC 702 698 . . 698 703 + HgiAI* GWGCWC 702 698 . . 699 702 + SetI ASST 702 698 . . 699 702 + CviJI RGCY 700 700 . . 699 702 + AluI AGCT 700 700 . . 714 718 - BccI CCATC 708 709 . . 720 723 + SetI ASST 723 719 . . 722 726 + MaeIII GTNAC 721 726 . . 718 722 - Hin4II* CCTTC 712 711 . . 737 740 + TaqI TCGA 737 739 . . 738 742 + Hpy99I CGWCG 742 737 . . 740 744 + Tsp4CI* ACNGT 742 741 . . 751 755 + Tsp45I GTSAC 750 755 . . 751 755 + MaeIII GTNAC 750 755 . . 753 764 + DrdI GACNNNNNNGTC 759 757 . . 754 758 + Tsp4CI* ACNGT 756 755 . . 765 770 + NlaIV GGNNCC 767 767 . . 765 770 + HgiCI* GGYRCC 765 769 . . 772 775 + TspEI AATT 771 775 . . 776 780 + Tsp4CI* ACNGT 778 777 . . 799 803 + BlsI GCNGC 801 800 . . 799 803 + BisI GCNGC 800 801 . . 799 803 + BbvI GCAGC 811 815 . . 799 803 + TseI GCWGC 799 802 . . 800 805 + PvuII CAGCTG 802 802 . . 800 805 + NspBII* CMGCKG 802 802 . . 801 804 + SetI ASST 804 800 . . 801 804 + CviJI RGCY 802 802 . . 801 804 + AluI AGCT 802 802 . . 810 814 + MboII GAAGA 822 821 . . 819 823 + Hpy99I CGWCG 823 818 . . 823 832 + BsaBI GATNNNNATC 827 827 . . 828 832 + TfiI GAWTC 828 831 . . 828 832 + HinfI GANTC 828 831 . . 831 836 + Hpy178III* TCNNGA 832 834 . . 843 846 + TspEI AATT 842 846 . . 849 860 + BdaI TGANNNNNNTCA 838 836 872 870 850 859 + XmnI GAANNNNTTC 854 854 . . 852 855 + SetI ASST 855 851 . . 852 855 + CviJI RGCY 853 853 . . 852 855 + AluI AGCT 853 853 . . 853 863 + MwoI GCNNNNNNNGC 859 856 . . 849 860 - BdaI TGANNNNNNTCA 872 870 838 836 869 873 + TspDTI ATGAA 884 882 . . 875 879 + Hpy188I TCNGA 877 876 . . 874 879 - MmeI TCCRAC 855 853 . . 888 891 + TspEI AATT 887 891 . . 891 896 + MmeI TCCRAC 916 914 . . 922 927 + PsiI TTATAA 924 924 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 359 568 # # AciI: # [CCGC] # 146 781 # # AclI: # [AACGTT] # 247 680 # # AgeI: # [ACCGGT] # 132 795 # # AhaIII*: # [TTTAAA] # 80 847 # # AluI: # [AGCT] # 51 74 102 700 # # ApoI: # [RAATTY] # 81 846 # # AsuII: # [TTCGAA] # 188 739 # # BaeI: # [ACNNNNGTAYC] # 33 33 119 253 489 # # BalI: # [TGGCCA] # 161 766 # # BbvI: # [GCAGC] # 116 811 # # BbvII*: # [GAAGAC] # 328 599 # # BccI: # [CCATC] # 219 708 # # BclI: # [TGATCA] # 270 657 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 55 838 # # BetI*: # [WCCGGW] # 132 363 432 # # BisI: # [GCNGC] # 127 800 # # BlsI: # [GCNGC] # 126 801 # # BsaBI: # [GATNNNNATC] # 100 827 # # BseMII: # [CTCAG] # 22 905 # # BsiYI*: # [CCNNNNNNNGG] # 68 371 488 # # BsmAI: # [GTCTC] # 29 898 # # BspCNI: # [CTCAG] # 23 904 # # BspMII*: # [TCCGGA] # 432 495 # # BsrDI: # [GCAATG] # 117 810 # # BssKI: # [CCNGG] # 340 587 # # BssNAI: # [GTATAC] # 360 567 # # BstKTI: # [GATC] # 174 267 486 # # CauII*: # [CCSGG] # 342 585 # # Cfr10I: # [RCCGGY] # 132 795 # # CfrI: # [YGGCCR] # 159 768 # # Csp6I: # [GTAC] # 414 513 # # CviAII: # [CATG] # 52 72 157 322 324 # # CviJI: # [RGCY] # 51 72 74 89 102 539 # # DdeI: # [CTNAG] # 9 22 261 635 # # DpnI: # [GATC] # 173 268 486 # # DrdI: # [GACNNNNNNGTC] # 168 759 # # DsaI*: # [CCRYGG] # 421 506 # # Eam1105I: # [GACNNNNNGTC] # 321 606 # # Ecl136II: # [GAGCTC] # 227 700 # # Eco57I: # [CTGAAG] # 74 853 # # Eco57MI: # [CTGRAG] # 74 853 # # EcoP15I: # [CAGCAG] # 389 538 # # EcoRV: # [GATATC] # 301 626 # # Esp3I: # [CGTCTC] # 29 898 # # FatI: # [CATG] # 52 72 156 323 324 # # HaeIII: # [GGCC] # 161 766 # # HgiAI*: # [GWGCWC] # 225 702 # # HgiCI*: # [GGYRCC] # 162 765 # # HgiJII*: # [GRGCYC] # 225 702 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 136 303 424 # # Hin4II*: # [CCTTC] # 100 162 215 450 # # HindII: # [GTYRAC] # 327 600 # # HinfI: # [GANTC] # 99 828 # # HpaII: # [CCGG] # 133 154 209 431 # # HphI: # [GGTGA] # 160 319 448 # # Hpy166II: # [GTNNAC] # 240 327 360 # # Hpy178III*: # [TCNNGA] # 30 52 95 162 252 336 # # Hpy188I: # [TCNGA] # 50 877 # # Hpy99I: # [CGWCG] # 81 104 742 # # Ksp632I*: # [CTCTTC] # 190 737 # # MaeI: # [CTAG] # 232 695 # # MaeII: # [ACGT] # 84 192 247 404 # # MaeIII: # [GTNAC] # 21 29 114 114 151 153 # 168 177 # # MboI: # [GATC] # 171 270 486 # # MboII: # [GAAGA] # 67 105 110 218 427 # # MmeI: # [TCCRAC] # 11 61 855 # # MnlI: # [CCTC] # 85 212 311 319 # # MseI: # [TTAA] # 18 24 79 198 239 369 # # MslI: # [CAYNNNNRTG] # 370 557 # # MwoI: # [GCNNNNNNNGC] # 68 859 # # NlaIII: # [CATG] # 52 72 160 319 324 # # NlaIV: # [GGNNCC] # 160 767 # # NspBII*: # [CMGCKG] # 125 802 # # PsiI: # [TTATAA] # 3 924 # # PvuII: # [CAGCTG] # 125 802 # # RsaI: # [GTAC] # 415 512 # # SacI: # [GAGCTC] # 225 702 # # SapI: # [GCTCTTC] # 190 737 # # ScrFI: # [CCNGG] # 342 585 # # SduI: # [GDGCHC] # 225 702 # # SecI*: # [CCNNGG] # 421 506 # # SetI: # [ASST] # 8 19 21 28 38 51 # 56 72 81 146 187 220 # # SfaNI: # [GCATC] # 34 893 # # TaiI: # [ACGT] # 84 192 244 407 # # TaqI: # [TCGA] # 63 125 190 549 # # TfiI: # [GAWTC] # 99 828 # # TseI: # [GCWGC] # 128 799 # # Tsp45I: # [GTSAC] # 177 182 568 # # Tsp4CI*: # [ACNGT] # 14 22 63 149 232 447 # # TspDTI: # [ATGAA] # 4 39 43 61 80 115 # 270 315 # # TspEI: # [AATT] # 27 28 28 40 45 71 # 81 82 82 129 314 # # TspGWI: # [ACGGA] # 53 324 550 # # TspRI: # [CASTGNN] # 35 892 # # VspI: # [ATTAAT] # 97 830 # # XmnI: # [GAANNNNTTC] # 73 854 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 927 # Reported_sequences: 1 # Reported_hitcount: 187 #---------------------------------------