Restriction Fragments for PHO2/YDL106C



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The currently selected gene/sequence is :

PHO2/YDL106C on chromosome IV from coordinates 271901 to 270222.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.3798.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 11 + MboII GAAGA 19 18 . . 10 15 + EcoRI GAATTC 10 14 . . 10 15 + ApoI RAATTY 10 14 . . 11 14 + TspEI AATT 10 14 . . 17 22 + SplI* CGTACG 17 21 . . 18 21 + Csp6I GTAC 18 20 . . 18 21 + RsaI GTAC 19 19 . . 22 25 + DpnI GATC 23 23 . . 22 25 + BstKTI GATC 24 22 . . 22 25 + MboI GATC 21 25 . . 24 29 + Hpy178III* TCNNGA 25 27 . . 32 35 + MseI TTAA 32 34 . . 47 52 + SfeI* CTRYAG 47 51 . . 65 68 + CviRI* TGCA 66 66 . . 70 73 + FatI CATG 69 73 . . 70 73 + NlaIII CATG 73 69 . . 70 73 + CviAII CATG 70 72 . . 100 103 + FatI CATG 99 103 . . 100 103 + NlaIII CATG 103 99 . . 100 103 + CviAII CATG 100 102 . . 102 107 + BclI TGATCA 102 106 . . 103 106 + DpnI GATC 104 104 . . 103 106 + BstKTI GATC 105 103 . . 103 106 + MboI GATC 102 106 . . 144 147 + TspEI AATT 143 147 . . 158 161 + SetI ASST 161 157 . . 159 163 + BssKI CCNGG 158 163 . . 159 163 + BseBI CCWGG 160 161 . . 159 163 + ScrFI CCNGG 160 161 . . 159 163 + EcoRII CCWGG 158 163 . . 160 165 + Eco57MI CTGRAG 181 179 . . 160 165 + GsuI CTGGAG 181 179 . . 163 168 + SduI GDGCHC 167 163 . . 163 168 + HgiAI* GWGCWC 167 163 . . 181 190 + MslI CAYNNNNRTG 185 185 . . 201 205 - TspDTI ATGAA 191 189 . . 205 208 + TaqI TCGA 205 207 . . 207 212 + BsmI GAATGC 213 211 . . 209 214 + EcoT22I ATGCAT 213 209 . . 210 213 + CviRI* TGCA 211 211 . . 211 215 + SfaNI GCATC 220 224 . . 214 218 + Hpy188I TCNGA 216 215 . . 221 226 + Hpy166II GTNNAC 223 223 . . 223 227 + BmgT120I GGNCC 224 225 . . 223 227 + AvaII GGWCC 223 226 . . 223 227 + AsuI* GGNCC 223 226 . . 225 228 + SetI ASST 228 224 . . 226 229 + MnlI CCTC 236 235 . . 233 236 + CviJI RGCY 234 234 . . 233 236 + HaeIII GGCC 234 234 . . 233 237 + BmgT120I GGNCC 234 235 . . 233 237 + AsuI* GGNCC 233 236 . . 240 243 - MnlI CCTC 233 232 . . 243 247 + HinfI GANTC 243 246 . . 243 247 - PleI GAGTC 237 238 . . 243 247 - MlyI GAGTC 237 237 . . 247 250 + FnuDII* CGCG 248 248 . . 248 251 + Hin6I GCGC 248 250 . . 248 251 + GlaI GCGC 249 249 . . 248 251 + HhaI GCGC 250 248 . . 256 260 + HphI GGTGA 268 267 . . 265 268 + MaeI CTAG 265 267 . . 283 288 + ApoI RAATTY 283 287 . . 284 287 + TspEI AATT 283 287 . . 297 302 + MmeI TCCRAC 322 320 . . 305 308 + MnlI CCTC 315 314 . . 331 335 + Hpy188I TCNGA 333 332 . . 333 338 + XhoII RGATCY 333 337 . . 333 338 + BglII AGATCT 333 337 . . 334 337 + DpnI GATC 335 335 . . 334 337 + BstKTI GATC 336 334 . . 334 337 + MboI GATC 333 337 . . 336 340 + Hpy188I TCNGA 338 337 . . 344 349 + BsmI GAATGC 350 348 . . 359 362 + TaiI ACGT 362 358 . . 359 362 + SetI ASST 362 358 . . 359 362 + MaeII ACGT 359 361 . . 362 366 + Hpy188I TCNGA 364 363 . . 365 370 + ApoI RAATTY 365 369 . . 366 369 + TspEI AATT 365 369 . . 375 379 + Hpy188I TCNGA 377 376 . . 383 387 + MboII GAAGA 395 394 . . 387 390 + SetI ASST 390 386 . . 387 390 + CviJI RGCY 388 388 . . 387 390 + AluI AGCT 388 388 . . 383 388 - Ksp632I* CTCTTC 378 381 . . 383 389 - SapI GCTCTTC 378 381 . . 392 395 + TspEI AATT 391 395 . . 396 399 - MnlI CCTC 389 388 . . 405 409 + BlsI GCNGC 407 406 . . 405 409 + BisI GCNGC 406 407 . . 405 409 + BbvI GCAGC 417 421 . . 405 409 + TseI GCWGC 405 408 . . 409 412 + FatI CATG 408 412 . . 409 412 + NlaIII CATG 412 408 . . 409 412 + CviAII CATG 409 411 . . 434 437 + MnlI CCTC 444 443 . . 438 442 + Tsp45I GTSAC 437 442 . . 438 442 + MaeIII GTNAC 437 442 . . 463 472 + BsaBI GATNNNNATC 467 467 . . 468 473 + McrI* CGRYCG 471 469 . . 468 473 + PvuI CGATCG 471 469 . . 469 472 + DpnI GATC 470 470 . . 469 472 + BstKTI GATC 471 469 . . 469 472 + MboI GATC 468 472 . . 478 483 + TatI WGTACW 478 482 . . 479 482 + Csp6I GTAC 479 481 . . 479 482 + RsaI GTAC 480 480 . . 490 493 + TspEI AATT 489 493 . . 496 500 + Tsp45I GTSAC 495 500 . . 496 500 + MaeIII GTNAC 495 500 . . 508 513 + TatI WGTACW 508 512 . . 508 513 + ScaI AGTACT 510 510 . . 509 512 + Csp6I GTAC 509 511 . . 509 512 + RsaI GTAC 510 510 . . 513 517 - TspDTI ATGAA 503 501 . . 515 519 - FokI GGATG 501 505 . . 515 519 - BseGI GGATG 514 512 . . 525 530 + Hpy178III* TCNNGA 526 528 . . 530 534 + TspDTI ATGAA 545 543 . . 532 536 + MboII GAAGA 544 543 . . 532 537 + BbvII* GAAGAC 539 543 . . 536 539 + SetI ASST 539 535 . . 548 551 + TaqI TCGA 548 550 . . 549 554 + McrI* CGRYCG 552 550 . . 551 555 + Tsp4CI* ACNGT 553 552 . . 559 564 + NspBII* CMGCKG 561 561 . . 559 562 + AciI CCGC 559 561 . . 592 602 + Hin4I GAYNNNNNVTC 583 578 615 610 602 613 + CspCI CAANNNNNGTGG 590 588 625 623 603 606 + TspEI AATT 602 606 . . 607 611 + Tsp4CI* ACNGT 609 608 . . 617 622 - MmeI TCCRAC 598 596 . . 628 632 + TspDTI ATGAA 643 641 . . 635 639 + TauI GCSGC 638 635 . . 635 639 + BlsI GCNGC 637 636 . . 635 639 + BisI GCNGC 636 637 . . 638 641 + Hin6I GCGC 638 640 . . 638 641 + GlaI GCGC 639 639 . . 638 641 + HhaI GCGC 640 638 . . 635 638 - AciI CCGC 635 637 . . 641 647 + TspRI CASTGNN 647 638 . . 644 647 + CviRI* TGCA 645 645 . . 641 646 - BtsI GCAGTG 640 638 . . 650 654 - Hin4II* CCTTC 644 643 . . 683 686 + SetI ASST 686 682 . . 700 705 + VspI ATTAAT 701 703 . . 701 704 + MseI TTAA 701 703 . . 715 718 + TaqI TCGA 715 717 . . 717 722 + BsmI GAATGC 723 721 . . 722 736 + XcmI CCANNNNNNNNNTGG 729 728 . . 730 733 + MseI TTAA 730 732 . . 741 746 + EcoRV GATATC 743 743 . . 756 760 + DdeI CTNAG 756 759 . . 756 760 + BspCNI CTCAG 769 767 . . 756 760 + BseMII CTCAG 770 768 . . 757 761 + Hpy188I TCNGA 759 758 . . 782 787 + DsaI* CCRYGG 782 786 . . 782 787 + StyI CCWWGG 782 786 . . 782 787 + NcoI CCATGG 782 786 . . 782 787 + SecI* CCNNGG 782 786 . . 783 786 + FatI CATG 782 786 . . 783 786 + NlaIII CATG 786 782 . . 783 786 + CviAII CATG 783 785 . . 800 805 + ApoI RAATTY 800 804 . . 801 804 + TspEI AATT 800 804 . . 805 810 + Ksp632I* CTCTTC 811 814 . . 809 814 + Hpy178III* TCNNGA 810 812 . . 806 810 - MboII GAAGA 798 797 . . 807 812 - Eco57MI CTGRAG 792 790 . . 812 817 + XhoII RGATCY 812 816 . . 807 812 - Eco57I CTGAAG 792 790 . . 812 816 + BinI* GGATC 820 821 . . 813 816 + DpnI GATC 814 814 . . 813 816 + BstKTI GATC 815 813 . . 813 816 + MboI GATC 812 816 . . 826 829 + MseI TTAA 826 828 . . 835 839 + Tsp45I GTSAC 834 839 . . 835 839 + MaeIII GTNAC 834 839 . . 836 841 + Hpy178III* TCNNGA 837 839 . . 841 844 + TspEI AATT 840 844 . . 867 871 + Hpy99I CGWCG 871 866 . . 884 888 + MaeIII GTNAC 883 888 . . 936 940 + Hpy99I CGWCG 940 935 . . 944 948 + Tsp4CI* ACNGT 946 945 . . 950 954 + TspDTI ATGAA 965 963 . . 952 956 + MboII GAAGA 964 963 . . 952 957 + BbvII* GAAGAC 959 963 . . 973 976 - MnlI CCTC 966 965 . . 981 984 - MnlI CCTC 974 973 . . 984 989 + BsmI GAATGC 990 988 . . 989 993 + DdeI CTNAG 989 992 . . 991 1001 + FalI AAGNNNNNCTT 982 977 1014 1009 988 994 - Bpu10I CCTNAGC 989 992 . . 991 1001 - FalI AAGNNNNNCTT 1014 1009 982 977 1007 1010 + TspEI AATT 1006 1010 . . 1013 1016 + SetI ASST 1016 1012 . . 1013 1016 + CviJI RGCY 1014 1014 . . 1013 1016 + AluI AGCT 1014 1014 . . 1018 1022 + Tsp4CI* ACNGT 1020 1019 . . 1021 1025 + Tsp45I GTSAC 1020 1025 . . 1021 1025 + MaeIII GTNAC 1020 1025 . . 1022 1026 - HphI GGTGA 1014 1013 . . 1028 1031 + DpnI GATC 1029 1029 . . 1028 1031 + BstKTI GATC 1030 1028 . . 1028 1031 + MboI GATC 1027 1031 . . 1031 1036 + TaqII CACCCA 1047 1045 . . 1030 1034 - HphI GGTGA 1022 1021 . . 1045 1050 + Hpy166II GTNNAC 1047 1047 . . 1053 1058 + AhaIII* TTTAAA 1055 1055 . . 1054 1057 + MseI TTAA 1054 1056 . . 1065 1070 + Hpy178III* TCNNGA 1066 1068 . . 1069 1078 + BsaBI GATNNNNATC 1073 1073 . . 1070 1074 + TspDTI ATGAA 1085 1083 . . 1072 1076 + MboII GAAGA 1084 1083 . . 1074 1079 + XhoII RGATCY 1074 1078 . . 1075 1078 + DpnI GATC 1076 1076 . . 1075 1078 + BstKTI GATC 1077 1075 . . 1075 1078 + MboI GATC 1074 1078 . . 1075 1079 - BinI* GGATC 1069 1070 . . 1080 1085 + ApoI RAATTY 1080 1084 . . 1081 1084 + TspEI AATT 1080 1084 . . 1093 1099 + TspRI CASTGNN 1099 1090 . . 1097 1101 + BmgT120I GGNCC 1098 1099 . . 1097 1101 + AvaII GGWCC 1097 1100 . . 1097 1101 + AsuI* GGNCC 1097 1100 . . 1101 1106 + NdeI CATATG 1102 1104 . . 1108 1118 + Hin4I GAYNNNNNVTC 1099 1094 1131 1126 1116 1120 + DdeI CTNAG 1116 1119 . . 1116 1120 + BspCNI CTCAG 1129 1127 . . 1116 1120 + BseMII CTCAG 1130 1128 . . 1117 1121 + Hpy188I TCNGA 1119 1118 . . 1122 1125 + SetI ASST 1125 1121 . . 1120 1124 - Hin4II* CCTTC 1114 1113 . . 1124 1129 + Hpy166II GTNNAC 1126 1126 . . 1124 1129 + AccI GTMKAC 1125 1127 . . 1130 1133 + SetI ASST 1133 1129 . . 1138 1142 + HgaI GACGC 1147 1152 . . 1155 1160 + AsuII TTCGAA 1156 1158 . . 1156 1159 + TaqI TCGA 1156 1158 . . 1159 1164 + SspI AATATT 1161 1161 . . 1165 1168 + MnlI CCTC 1175 1174 . . 1179 1182 + SetI ASST 1182 1178 . . 1181 1186 + Hpy166II GTNNAC 1183 1183 . . 1195 1198 + MseI TTAA 1195 1197 . . 1199 1203 + TfiI GAWTC 1199 1202 . . 1199 1203 + HinfI GANTC 1199 1202 . . 1202 1207 + Hpy178III* TCNNGA 1203 1205 . . 1202 1207 + BspHI TCATGA 1202 1206 . . 1203 1206 + FatI CATG 1202 1206 . . 1203 1206 + NlaIII CATG 1206 1202 . . 1203 1206 + CviAII CATG 1203 1205 . . 1204 1208 + TspDTI ATGAA 1219 1217 . . 1201 1205 - TspDTI ATGAA 1191 1189 . . 1206 1211 + EcoRI GAATTC 1206 1210 . . 1206 1211 + ApoI RAATTY 1206 1210 . . 1207 1210 + TspEI AATT 1206 1210 . . 1215 1218 + TspEI AATT 1214 1218 . . 1218 1223 + XbaI TCTAGA 1218 1222 . . 1218 1223 + Hpy178III* TCNNGA 1219 1221 . . 1219 1222 + MaeI CTAG 1219 1221 . . 1222 1232 + Hin4I GAYNNNNNVTC 1213 1208 1245 1240 1222 1232 - Hin4I GAYNNNNNVTC 1245 1240 1213 1208 1234 1237 + TaqI TCGA 1234 1236 . . 1238 1242 + TspDTI ATGAA 1253 1251 . . 1242 1247 + SspI AATATT 1244 1244 . . 1248 1251 + SetI ASST 1251 1247 . . 1254 1257 + DpnI GATC 1255 1255 . . 1254 1257 + BstKTI GATC 1256 1254 . . 1254 1257 + MboI GATC 1253 1257 . . 1266 1269 + DpnI GATC 1267 1267 . . 1266 1269 + BstKTI GATC 1268 1266 . . 1266 1269 + MboI GATC 1265 1269 . . 1266 1270 - BinI* GGATC 1260 1261 . . 1272 1278 + TspRI CASTGNN 1278 1269 . . 1276 1280 + BlsI GCNGC 1278 1277 . . 1276 1280 + BisI GCNGC 1277 1278 . . 1276 1280 + TseI GCWGC 1276 1279 . . 1277 1282 + PstI CTGCAG 1281 1277 . . 1272 1277 - BtsI GCAGTG 1271 1269 . . 1277 1282 + SfeI* CTRYAG 1277 1281 . . 1278 1281 + CviRI* TGCA 1279 1279 . . 1274 1279 - EcoP15I CAGCAG 1246 1248 . . 1276 1280 - BbvI GCAGC 1263 1267 . . 1294 1299 + SspI AATATT 1296 1296 . . 1303 1306 + MnlI CCTC 1313 1312 . . 1311 1315 + Hpy188I TCNGA 1313 1312 . . 1320 1325 + ApoI RAATTY 1320 1324 . . 1321 1324 + TspEI AATT 1320 1324 . . 1329 1332 + CviRI* TGCA 1330 1330 . . 1336 1340 + HinfI GANTC 1336 1339 . . 1336 1340 - PleI GAGTC 1330 1331 . . 1336 1340 - MlyI GAGTC 1330 1330 . . 1353 1358 + ApoI RAATTY 1353 1357 . . 1354 1357 + TspEI AATT 1353 1357 . . 1360 1364 + MboII GAAGA 1372 1371 . . 1363 1367 + TfiI GAWTC 1363 1366 . . 1363 1367 + HinfI GANTC 1363 1366 . . 1369 1374 + Ksp632I* CTCTTC 1375 1378 . . 1370 1374 - MboII GAAGA 1362 1361 . . 1376 1379 + TaqI TCGA 1376 1378 . . 1373 1377 - MboII GAAGA 1365 1364 . . 1378 1381 + DpnI GATC 1379 1379 . . 1378 1381 + BstKTI GATC 1380 1378 . . 1378 1387 + BsaBI GATNNNNATC 1382 1382 . . 1378 1381 + MboI GATC 1377 1381 . . 1380 1385 + Hpy178III* TCNNGA 1381 1383 . . 1380 1385 + BspHI TCATGA 1380 1384 . . 1381 1384 + FatI CATG 1380 1384 . . 1381 1384 + NlaIII CATG 1384 1380 . . 1381 1384 + CviAII CATG 1381 1383 . . 1384 1394 + Hin4I GAYNNNNNVTC 1375 1370 1407 1402 1384 1387 + DpnI GATC 1385 1385 . . 1384 1387 + BstKTI GATC 1386 1384 . . 1384 1387 + MboI GATC 1383 1387 . . 1393 1396 + TaqI TCGA 1393 1395 . . 1384 1394 - Hin4I GAYNNNNNVTC 1407 1402 1375 1370 1399 1402 + TaqI TCGA 1399 1401 . . 1403 1408 + TsoI TARCCA 1419 1417 . . 1418 1422 + TspGWI ACGGA 1433 1431 . . 1426 1429 + CviJI RGCY 1427 1427 . . 1439 1444 + ZraI GACGTC 1441 1441 . . 1439 1444 + AcyI GRCGYC 1440 1442 . . 1439 1444 + AatII GACGTC 1443 1439 . . 1440 1443 + TaiI ACGT 1443 1439 . . 1440 1443 + SetI ASST 1443 1439 . . 1440 1443 + MaeII ACGT 1440 1442 . . 1446 1449 + SetI ASST 1449 1445 . . 1446 1449 + CviJI RGCY 1447 1447 . . 1446 1449 + AluI AGCT 1447 1447 . . 1447 1452 + Cac8I GCNNGC 1449 1449 . . 1447 1452 + BmtI GCTAGC 1451 1447 . . 1447 1452 + NheI GCTAGC 1447 1451 . . 1448 1451 + MaeI CTAG 1448 1450 . . 1455 1459 + FokI GGATG 1468 1472 . . 1455 1459 + BseGI GGATG 1461 1459 . . 1468 1471 + TspEI AATT 1467 1471 . . 1473 1477 + BsrI ACTGG 1478 1476 . . 1476 1481 - BciVI GTATCC 1470 1469 . . 1483 1487 + Tsp4CI* ACNGT 1485 1484 . . 1486 1491 + Hpy166II GTNNAC 1488 1488 . . 1486 1491 + HindII GTYRAC 1488 1488 . . 1477 1487 - BaeI ACNNNNGTAYC 1502 1497 1469 1464 1518 1529 + BdaI TGANNNNNNTCA 1507 1505 1541 1539 1519 1524 + BseRI GAGGAG 1534 1532 . . 1519 1522 - MnlI CCTC 1512 1511 . . 1524 1528 + TfiI GAWTC 1524 1527 . . 1524 1528 + HinfI GANTC 1524 1527 . . 1518 1529 - BdaI TGANNNNNNTCA 1541 1539 1507 1505 1531 1534 + MaeI CTAG 1531 1533 . . 1534 1537 + Hin6I GCGC 1534 1536 . . 1534 1537 + GlaI GCGC 1535 1535 . . 1534 1537 + HhaI GCGC 1536 1534 . . 1549 1554 + MmeI TCCRAC 1574 1572 . . 1555 1559 - SfaNI GCATC 1545 1549 . . 1569 1572 + CviRI* TGCA 1570 1570 . . 1581 1586 + BclI TGATCA 1581 1585 . . 1582 1585 + DpnI GATC 1583 1583 . . 1582 1585 + BstKTI GATC 1584 1582 . . 1582 1585 + MboI GATC 1581 1585 . . 1610 1615 + Hpy166II GTNNAC 1612 1612 . . 1626 1631 + Hpy178III* TCNNGA 1627 1629 . . 1625 1630 - Eco57MI CTGRAG 1610 1608 . . 1625 1630 - GsuI CTGGAG 1610 1608 . . 1638 1642 + MboII GAAGA 1650 1649 . . 1670 1674 - BccI CCATC 1664 1665 . . #--------------------------------------- # # # AatII: # [GACGTC] # 237 1443 # # AccI: # [GTMKAC] # 555 1125 # # AciI: # [CCGC] # 76 559 1045 # # AcyI: # [GRCGYC] # 240 1440 # # AhaIII*: # [TTTAAA] # 625 1055 # # AluI: # [AGCT] # 233 388 433 626 # # ApoI: # [RAATTY] # 10 33 82 114 126 273 # 280 327 435 # # AsuI*: # [GGNCC] # 10 223 583 864 # # AsuII: # [TTCGAA] # 524 1156 # # AvaII: # [GGWCC] # 223 583 874 # # BaeI: # [ACNNNNGTAYC] # 33 178 1469 # # BbvI: # [GCAGC] # 417 417 846 # # BbvII*: # [GAAGAC] # 420 539 721 # # BccI: # [CCATC] # 16 1664 # # BciVI: # [GTATCC] # 210 1470 # # BclI: # [TGATCA] # 99 102 1479 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 139 1507 # # BglII: # [AGATCT] # 333 1347 # # BinI*: # [GGATC] # 191 249 420 820 # # BisI: # [GCNGC] # 230 403 406 641 # # BlsI: # [GCNGC] # 230 402 407 641 # # BmgT120I: # [GGNCC] # 10 224 582 864 # # BmtI: # [GCTAGC] # 229 1451 # # Bpu10I: # [CCTNAGC] # 691 989 # # BsaBI: # [GATNNNNATC] # 298 309 467 606 # # BseBI: # [CCWGG] # 160 1520 # # BseGI: # [GGATG] # 219 514 947 # # BseMII: # [CTCAG] # 360 550 770 # # BseRI: # [GAGGAG] # 146 1534 # # BsmI: # [GAATGC] # 137 213 267 373 690 # # BspCNI: # [CTCAG] # 360 551 769 # # BspHI: # [TCATGA] # 178 300 1202 # # BsrI: # [ACTGG] # 202 1478 # # BssKI: # [CCNGG] # 158 1522 # # BstKTI: # [GATC] # 6 12 24 47 81 96 # 112 135 179 198 215 231 # 344 # # BtsI: # [GCAGTG] # 409 631 640 # # Cac8I: # [GCNNGC] # 231 1449 # # Csp6I: # [GTAC] # 18 30 461 1171 # # CspCI: # [CAANNNNNGTGG] # 35 590 1055 # # CviAII: # [CATG] # 30 70 178 299 309 374 # 420 # # CviJI: # [RGCY] # 20 154 233 234 413 626 # # CviRI*: # [TGCA] # 51 66 110 145 240 434 # 634 # # DdeI: # [CTNAG] # 127 233 564 756 # # DpnI: # [GATC] # 6 12 23 47 81 97 # 112 135 179 198 215 231 # 344 # # DsaI*: # [CCRYGG] # 782 898 # # Eco57I: # [CTGAAG] # 792 888 # # Eco57MI: # [CTGRAG] # 70 181 611 818 # # EcoP15I: # [CAGCAG] # 434 1246 # # EcoRI: # [GAATTC] # 10 474 1196 # # EcoRII: # [CCWGG] # 158 1522 # # EcoRV: # [GATATC] # 743 937 # # EcoT22I: # [ATGCAT] # 213 1467 # # FalI: # [AAGNNNNNCTT] # 0 0 32 666 982 # # FatI: # [CATG] # 30 69 178 300 309 374 # 420 # # FnuDII*: # [CGCG] # 248 1432 # # FokI: # [GGATG] # 212 501 967 # # GlaI: # [GCGC] # 145 249 390 896 # # GsuI: # [CTGGAG] # 70 181 1429 # # HaeIII: # [GGCC] # 234 1446 # # HgaI: # [GACGC] # 533 1147 # # HgiAI*: # [GWGCWC] # 167 1513 # # HhaI: # [GCGC] # 144 250 390 896 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 32 32 82 130 273 484 # 583 # # Hin4II*: # [CCTTC] # 470 566 644 # # Hin6I: # [GCGC] # 146 248 390 896 # # HindII: # [GTYRAC] # 192 1488 # # HinfI: # [GANTC] # 27 137 156 161 243 956 # # HphI: # [GGTGA] # 8 268 658 746 # # Hpy166II: # [GTNNAC] # 57 68 79 124 223 305 # 824 # # Hpy178III*: # [TCNNGA] # 16 25 27 53 137 162 # 229 246 284 501 # # Hpy188I: # [TCNGA] # 5 13 26 117 194 216 # 360 367 382 # # Hpy99I: # [CGWCG] # 69 740 871 # # Ksp632I*: # [CTCTTC] # 305 378 433 564 # # MaeI: # [CTAG] # 83 149 229 265 954 # # MaeII: # [ACGT] # 240 359 1081 # # MaeIII: # [GTNAC] # 49 58 137 339 437 660 # # MboI: # [GATC] # 6 12 21 47 81 99 # 112 135 179 198 215 231 # 344 # # MboII: # [GAAGA] # 3 7 19 30 120 149 # 166 254 278 278 376 # # McrI*: # [CGRYCG] # 81 471 1128 # # MlyI: # [GAGTC] # 237 350 1093 # # MmeI: # [TCCRAC] # 106 276 322 976 # # MnlI: # [CCTC] # 3 8 55 74 79 138 # 168 199 201 233 522 # # MseI: # [TTAA] # 29 32 96 141 228 485 # 669 # # MslI: # [CAYNNNNRTG] # 185 1495 # # NcoI: # [CCATGG] # 782 898 # # NdeI: # [CATATG] # 578 1102 # # NheI: # [GCTAGC] # 233 1447 # # NlaIII: # [CATG] # 30 73 178 296 309 374 # 420 # # NspBII*: # [CMGCKG] # 561 1119 # # PleI: # [GAGTC] # 237 350 1093 # # PstI: # [CTGCAG] # 399 1281 # # PvuI: # [CGATCG] # 471 1209 # # RsaI: # [GTAC] # 19 30 461 1170 # # SapI: # [GCTCTTC] # 378 1302 # # ScaI: # [AGTACT] # 510 1170 # # ScrFI: # [CCNGG] # 160 1520 # # SduI: # [GDGCHC] # 167 1513 # # SecI*: # [CCNNGG] # 782 898 # # SetI: # [ASST] # 6 8 28 49 67 69 # 109 134 147 149 161 192 # 231 330 # # SfaNI: # [GCATC] # 135 220 1325 # # SfeI*: # [CTRYAG] # 47 403 1230 # # SplI*: # [CGTACG] # 17 1663 # # SspI: # [AATATT] # 52 83 384 1161 # # StyI: # [CCWWGG] # 782 898 # # TaiI: # [ACGT] # 237 362 1081 # # TaqI: # [TCGA] # 6 17 78 142 167 205 # 281 343 441 # # TaqII: # [CACCCA] # 633 1047 # # TatI: # [WGTACW] # 30 478 1172 # # TauI: # [GCSGC] # 638 1042 # # TfiI: # [GAWTC] # 156 161 164 1199 # # TseI: # [GCWGC] # 404 405 871 # # TsoI: # [TARCCA] # 261 1419 # # Tsp45I: # [GTSAC] # 58 186 339 437 660 # # Tsp4CI*: # [ACNGT] # 56 74 195 337 465 553 # # TspDTI: # [ATGAA] # 28 34 42 98 106 120 # 191 312 322 427 # # TspEI: # [AATT] # 8 10 26 33 40 74 # 82 98 106 113 114 126 # 133 140 166 198 213 # # TspGWI: # [ACGGA] # 247 1433 # # TspRI: # [CASTGNN] # 179 402 452 647 # # VspI: # [ATTAAT] # 701 979 # # XbaI: # [TCTAGA] # 462 1218 # # XcmI: # [CCANNNNNNNNNTGG] # 729 951 # # XhoII: # [RGATCY] # 262 333 479 606 # # ZraI: # [GACGTC] # 239 1441 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1680 # Reported_sequences: 1 # Reported_hitcount: 366 #---------------------------------------