Restriction Fragments for MSH3/YCR092C



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The currently selected gene/sequence is :

MSH3/YCR092C on chromosome III from coordinates 279820 to 276764.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.6949.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 10 + BslFI GGGAC 20 24 . . 4 7 - AciI CCGC 4 6 . . 25 28 + SetI ASST 28 24 . . 23 28 - BspMI ACCTGC 14 18 . . 32 37 + Hpy178III* TCNNGA 33 35 . . 36 40 - Hin4II* CCTTC 30 29 . . 40 43 - AciI CCGC 40 42 . . 49 53 + Hpy188I TCNGA 51 50 . . 53 56 + SetI ASST 56 52 . . 53 56 + CviJI RGCY 54 54 . . 53 56 + AluI AGCT 54 54 . . 57 61 + HgaI GACGC 66 71 . . 76 89 + AjuI GAANNNNNNNTTGG 68 63 100 95 82 85 - AciI CCGC 82 84 . . 85 90 - MmeI TCCRAC 66 64 . . 94 99 + HaeII RGCGCY 98 94 . . 95 98 + Hin6I GCGC 95 97 . . 95 98 + GlaI GCGC 96 96 . . 95 105 + MwoI GCNNNNNNNGC 101 98 . . 95 98 + HhaI GCGC 97 95 . . 106 110 + TfiI GAWTC 106 109 . . 106 110 + HinfI GANTC 106 109 . . 110 114 + BccI CCATC 118 119 . . 123 129 + TspRI CASTGNN 129 120 . . 128 132 + TspDTI ATGAA 143 141 . . 130 134 + MboII GAAGA 142 141 . . 130 135 - Ksp632I* CTCTTC 125 128 . . 133 136 - MnlI CCTC 126 125 . . 136 146 + Hin4I GAYNNNNNVTC 127 122 159 154 139 142 + TspEI AATT 138 142 . . 136 146 - Hin4I GAYNNNNNVTC 159 154 127 122 145 149 - MboII GAAGA 137 136 . . 153 156 + CviRI* TGCA 154 154 . . 154 159 + Cac8I GCNNGC 156 156 . . 163 167 + Tsp4CI* ACNGT 165 164 . . 166 170 + MaeIII GTNAC 165 170 . . 178 181 + SetI ASST 181 177 . . 178 181 + CviJI RGCY 179 179 . . 178 181 + AluI AGCT 179 179 . . 184 194 + BsiYI* CCNNNNNNNGG 190 187 . . 194 199 + BtsI GCAGTG 201 199 . . 195 201 + TspRI CASTGNN 201 192 . . 202 206 - MboII GAAGA 194 193 . . 210 215 + EcoRI GAATTC 210 214 . . 210 215 + ApoI RAATTY 210 214 . . 211 214 + TspEI AATT 210 214 . . 213 218 + AsuII TTCGAA 214 216 . . 214 217 + TaqI TCGA 214 216 . . 219 224 + ApoI RAATTY 219 223 . . 220 223 + TspEI AATT 219 223 . . 223 227 + Hpy188I TCNGA 225 224 . . 232 237 + SpeI ACTAGT 232 236 . . 233 236 + MaeI CTAG 233 235 . . 239 242 + Csp6I GTAC 239 241 . . 239 242 + RsaI GTAC 240 240 . . 244 247 + TaqI TCGA 244 246 . . 254 257 + MseI TTAA 254 256 . . 272 276 + DdeI CTNAG 272 275 . . 278 281 + TspEI AATT 277 281 . . 292 296 + TspDTI ATGAA 307 305 . . 308 312 + TspDTI ATGAA 323 321 . . 321 324 + CviJI RGCY 322 322 . . 319 322 - MnlI CCTC 312 311 . . 323 328 + Eco57MI CTGRAG 344 342 . . 323 328 + Eco57I CTGAAG 344 342 . . 325 329 + MboII GAAGA 337 336 . . 334 337 - MnlI CCTC 327 326 . . 337 341 + MboII GAAGA 349 348 . . 337 342 - Ksp632I* CTCTTC 332 335 . . 340 343 - MnlI CCTC 333 332 . . 343 347 + HphI GGTGA 355 354 . . 346 349 - MnlI CCTC 339 338 . . 349 353 + MboII GAAGA 361 360 . . 369 372 + CviJI RGCY 370 370 . . 378 382 - BslFI GGGAC 363 367 . . 383 388 + SfeI* CTRYAG 383 387 . . 401 406 + StyI CCWWGG 401 405 . . 401 406 + SecI* CCNNGG 401 405 . . 413 416 + SetI ASST 416 412 . . 414 418 + HphI GGTGA 426 425 . . 419 425 + PpuMI RGGWCCY 420 423 . . 419 425 + DraII RGGNCCY 420 423 . . 420 424 + BmgT120I GGNCC 421 422 . . 420 424 + AvaII GGWCC 420 423 . . 420 424 + AsuI* GGNCC 420 423 . . 417 421 - Hin4II* CCTTC 411 410 . . 422 425 + SetI ASST 425 421 . . 430 435 + EcoT22I ATGCAT 434 430 . . 431 434 + CviRI* TGCA 432 432 . . 432 436 + SfaNI GCATC 441 445 . . 466 469 + CviJI RGCY 467 467 . . 473 478 + TatI WGTACW 473 477 . . 474 477 + Csp6I GTAC 474 476 . . 474 477 + RsaI GTAC 475 475 . . 486 489 + CviRI* TGCA 487 487 . . 487 497 + MwoI GCNNNNNNNGC 493 490 . . 487 497 + BstAPI GCANNNNNTGC 493 490 . . 492 496 + FokI GGATG 505 509 . . 492 496 + BseGI GGATG 498 496 . . 490 493 - MnlI CCTC 483 482 . . 495 498 + CviRI* TGCA 496 496 . . 493 497 - SfaNI GCATC 483 487 . . 499 503 + MaeIII GTNAC 498 503 . . 502 506 + Tsp4CI* ACNGT 504 503 . . 535 539 + BssKI CCNGG 534 539 . . 535 539 + BseBI CCWGG 536 537 . . 535 539 + ScrFI CCNGG 536 537 . . 535 539 + EcoRII CCWGG 534 539 . . 542 545 + TspEI AATT 541 545 . . 550 555 + ClaI ATCGAT 551 553 . . 551 554 + TaqI TCGA 551 553 . . 556 560 + PleI GAGTC 564 565 . . 556 560 + MlyI GAGTC 565 565 . . 556 560 + HinfI GANTC 556 559 . . 565 568 + MnlI CCTC 575 574 . . 566 571 + SmlI CTYRAG 566 570 . . 567 572 + Hpy178III* TCNNGA 568 570 . . 571 580 + BsaBI GATNNNNATC 575 575 . . 566 571 - Bce83I* CTTGAG 551 549 . . 574 577 + CviRI* TGCA 575 575 . . 594 597 + Csp6I GTAC 594 596 . . 594 597 + RsaI GTAC 595 595 . . 596 600 + Tsp4CI* ACNGT 598 597 . . 605 611 + PfoI TCCNGGA 605 610 . . 606 610 + BssKI CCNGG 605 610 . . 606 610 + ScrFI CCNGG 607 608 . . 606 610 + CauII* CCSGG 607 608 . . 607 610 + HpaII CCGG 607 609 . . 609 613 + FokI GGATG 622 626 . . 609 613 + BseGI GGATG 615 613 . . 614 618 + Hpy188I TCNGA 616 615 . . 619 622 + MseI TTAA 619 621 . . 622 627 + AclI AACGTT 623 625 . . 623 626 + TaiI ACGT 626 622 . . 623 626 + SetI ASST 626 622 . . 623 626 + MaeII ACGT 623 625 . . 625 630 + Hpy166II GTNNAC 627 627 . . 629 632 + SetI ASST 632 628 . . 631 634 + MaeI CTAG 631 633 . . 627 631 - HphI GGTGA 619 618 . . 636 640 - BsmAI GTCTC 630 634 . . 644 647 + CviRI* TGCA 645 645 . . 645 649 + SfaNI GCATC 654 658 . . 652 655 + TspEI AATT 651 655 . . 654 659 + AhaIII* TTTAAA 656 656 . . 655 658 + MseI TTAA 655 657 . . 659 662 + SetI ASST 662 658 . . 665 670 + DsaI* CCRYGG 665 669 . . 665 670 + SecI* CCNNGG 665 669 . . 664 668 - BceAI ACGGC 649 651 . . 675 680 + Cac8I GCNNGC 677 677 . . 688 693 + HaeII RGCGCY 692 688 . . 688 693 + Eco47III AGCGCT 690 690 . . 689 692 + Hin6I GCGC 689 691 . . 689 692 + GlaI GCGC 690 690 . . 689 692 + HhaI GCGC 691 689 . . 695 698 + MseI TTAA 695 697 . . 703 706 + FatI CATG 702 706 . . 703 706 + NlaIII CATG 706 702 . . 703 706 + CviAII CATG 703 705 . . 706 709 + DpnI GATC 707 707 . . 706 709 + BstKTI GATC 708 706 . . 706 709 + MboI GATC 705 709 . . 709 713 + BssKI CCNGG 708 713 . . 709 713 + BseBI CCWGG 710 711 . . 709 713 + ScrFI CCNGG 710 711 . . 709 713 + EcoRII CCWGG 708 713 . . 706 710 - BinI* GGATC 700 701 . . 711 714 + SetI ASST 714 710 . . 712 717 + NlaIV GGNNCC 714 714 . . 712 717 + HgiCI* GGYRCC 712 716 . . 715 720 + Cac8I GCNNGC 717 717 . . 719 729 + MwoI GCNNNNNNNGC 725 722 . . 740 749 + XmnI GAANNNNTTC 744 744 . . 765 768 + SetI ASST 768 764 . . 765 768 + CviJI RGCY 766 766 . . 765 768 + AluI AGCT 766 766 . . 779 782 + MseI TTAA 779 781 . . 781 784 + TspEI AATT 780 784 . . 787 790 + SetI ASST 790 786 . . 795 801 + SauI* CCTNAGG 796 799 . . 796 800 + DdeI CTNAG 796 799 . . 807 810 + TaiI ACGT 810 806 . . 807 810 + SetI ASST 810 806 . . 807 810 + MaeII ACGT 807 809 . . 817 821 + HphI GGTGA 829 828 . . 827 831 + Tsp4CI* ACNGT 829 828 . . 837 840 + CviJI RGCY 838 838 . . 843 847 - BslFI GGGAC 828 832 . . 848 852 + Tsp45I GTSAC 847 852 . . 848 852 + MaeIII GTNAC 847 852 . . 849 860 + BdaI TGANNNNNNTCA 838 836 872 870 851 854 + TaiI ACGT 854 850 . . 851 854 + SetI ASST 854 850 . . 851 854 + MaeII ACGT 851 853 . . 853 856 + Csp6I GTAC 853 855 . . 853 856 + RsaI GTAC 854 854 . . 849 860 - BdaI TGANNNNNNTCA 872 870 838 836 866 869 + SetI ASST 869 865 . . 870 873 + CviJI RGCY 871 871 . . 869 874 - TsoI TARCCA 859 857 . . 875 880 + SspI AATATT 877 877 . . 883 886 + MseI TTAA 883 885 . . 885 888 + TspEI AATT 884 888 . . 895 898 + TspEI AATT 894 898 . . 896 903 + SwaI ATTTAAAT 899 899 . . 897 902 + AhaIII* TTTAAA 899 899 . . 898 901 + MseI TTAA 898 900 . . 923 927 + TspDTI ATGAA 938 936 . . 925 930 + ApoI RAATTY 925 929 . . 926 929 + TspEI AATT 925 929 . . 931 935 + MboII GAAGA 943 942 . . 935 938 + CviJI RGCY 936 936 . . 931 936 - Ksp632I* CTCTTC 926 929 . . 931 937 - SapI GCTCTTC 926 929 . . 958 963 + SfeI* CTRYAG 958 962 . . 963 972 + BsaBI GATNNNNATC 967 967 . . 968 972 + TfiI GAWTC 968 971 . . 968 972 + HinfI GANTC 968 971 . . 974 979 + SspI AATATT 976 976 . . 983 986 + CviJI RGCY 984 984 . . 986 1000 + XcmI CCANNNNNNNNNTGG 993 992 . . 993 998 + TatI WGTACW 993 997 . . 993 998 + ScaI AGTACT 995 995 . . 994 997 + Csp6I GTAC 994 996 . . 994 997 + RsaI GTAC 995 995 . . 996 1000 + BsrI ACTGG 1001 999 . . 1012 1015 + DpnI GATC 1013 1013 . . 1012 1015 + BstKTI GATC 1014 1012 . . 1012 1015 + MboI GATC 1011 1015 . . 1014 1018 - MboII GAAGA 1006 1005 . . 1023 1028 + BspLU11I* ACATGT 1023 1027 . . 1023 1028 + NspI RCATGY 1027 1023 . . 1023 1028 + AflIII ACRYGT 1023 1027 . . 1024 1027 + FatI CATG 1023 1027 . . 1024 1027 + NlaIII CATG 1027 1023 . . 1024 1027 + CviAII CATG 1024 1026 . . 1032 1041 + XmnI GAANNNNTTC 1036 1036 . . 1033 1038 + ApoI RAATTY 1033 1037 . . 1034 1037 + TspEI AATT 1033 1037 . . 1052 1055 + FatI CATG 1051 1055 . . 1052 1055 + NlaIII CATG 1055 1051 . . 1052 1055 + CviAII CATG 1052 1054 . . 1050 1054 - TspDTI ATGAA 1040 1038 . . 1060 1063 + MseI TTAA 1060 1062 . . 1087 1091 + MboII GAAGA 1099 1098 . . 1090 1093 + DpnI GATC 1091 1091 . . 1090 1093 + BstKTI GATC 1092 1090 . . 1090 1093 + MboI GATC 1089 1093 . . 1093 1096 + FatI CATG 1092 1096 . . 1093 1096 + NlaIII CATG 1096 1092 . . 1093 1096 + CviAII CATG 1093 1095 . . 1117 1121 + TspDTI ATGAA 1132 1130 . . 1122 1133 + BdaI TGANNNNNNTCA 1111 1109 1145 1143 1127 1132 + ApoI RAATTY 1127 1131 . . 1128 1131 + TspEI AATT 1127 1131 . . 1122 1133 - BdaI TGANNNNNNTCA 1145 1143 1111 1109 1142 1147 + Esp3I CGTCTC 1148 1152 . . 1143 1147 + BsmAI GTCTC 1148 1152 . . 1140 1144 - BceAI ACGGC 1125 1127 . . 1155 1159 + DdeI CTNAG 1155 1158 . . 1163 1167 + DdeI CTNAG 1163 1166 . . 1177 1182 + NdeI CATATG 1178 1180 . . 1187 1192 + TatI WGTACW 1187 1191 . . 1188 1191 + Csp6I GTAC 1188 1190 . . 1188 1191 + RsaI GTAC 1189 1189 . . 1202 1205 + SetI ASST 1205 1201 . . 1203 1207 + HphI GGTGA 1215 1214 . . 1200 1205 - BspMI ACCTGC 1191 1195 . . 1200 1206 - AarI CACCTGC 1191 1195 . . 1212 1216 + TfiI GAWTC 1212 1215 . . 1212 1216 + HinfI GANTC 1212 1215 . . 1216 1220 + Hin4II* CCTTC 1226 1225 . . 1232 1236 + Hin4II* CCTTC 1242 1241 . . 1237 1241 + SfaNI GCATC 1246 1250 . . 1249 1254 + NdeI CATATG 1250 1252 . . 1254 1258 + MaeIII GTNAC 1253 1258 . . 1261 1271 + Hin4I GAYNNNNNVTC 1252 1247 1284 1279 1261 1264 + DpnI GATC 1262 1262 . . 1261 1264 + BstKTI GATC 1263 1261 . . 1261 1264 + MboI GATC 1260 1264 . . 1261 1265 - BinI* GGATC 1255 1256 . . 1274 1277 + CviRI* TGCA 1275 1275 . . 1296 1307 + BstXI CCANNNNNNTGG 1303 1299 . . 1297 1306 + MslI CAYNNNNRTG 1301 1301 . . 1297 1300 + FatI CATG 1296 1300 . . 1297 1300 + NlaIII CATG 1300 1296 . . 1297 1300 + CviAII CATG 1297 1299 . . 1300 1304 - BccI CCATC 1294 1295 . . 1311 1314 + SetI ASST 1314 1310 . . 1324 1329 - TsoI TARCCA 1314 1312 . . 1332 1336 + BmgT120I GGNCC 1333 1334 . . 1332 1336 + AvaII GGWCC 1332 1335 . . 1332 1336 + AsuI* GGNCC 1332 1335 . . 1357 1360 + MseI TTAA 1357 1359 . . 1372 1381 + XmnI GAANNNNTTC 1376 1376 . . 1377 1381 + TfiI GAWTC 1377 1380 . . 1377 1381 + HinfI GANTC 1377 1380 . . 1386 1389 + SetI ASST 1389 1385 . . 1398 1403 + TatI WGTACW 1398 1402 . . 1398 1403 + Bsp1407I TGTACA 1398 1402 . . 1399 1404 + Hpy166II GTNNAC 1401 1401 . . 1399 1402 + Csp6I GTAC 1399 1401 . . 1399 1402 + RsaI GTAC 1400 1400 . . 1407 1410 + TspEI AATT 1406 1410 . . 1411 1415 + MboII GAAGA 1423 1422 . . 1412 1422 + FalI AAGNNNNNCTT 1403 1398 1435 1430 1416 1420 + TauI GCSGC 1419 1416 . . 1411 1416 - Ksp632I* CTCTTC 1406 1409 . . 1416 1420 + BlsI GCNGC 1418 1417 . . 1416 1420 + BisI GCNGC 1417 1418 . . 1411 1417 - SapI GCTCTTC 1406 1409 . . 1418 1421 + CviJI RGCY 1419 1419 . . 1416 1419 - AciI CCGC 1416 1418 . . 1414 1419 - BsrBI CCGCTC 1416 1416 . . 1412 1422 - FalI AAGNNNNNCTT 1435 1430 1403 1398 1423 1427 - SfaNI GCATC 1413 1417 . . 1435 1438 + CviRI* TGCA 1436 1436 . . 1444 1448 + Hpy188I TCNGA 1446 1445 . . 1442 1446 - FokI GGATG 1428 1432 . . 1442 1446 - BseGI GGATG 1441 1439 . . 1457 1461 + Tsp4CI* ACNGT 1459 1458 . . 1471 1475 + TfiI GAWTC 1471 1474 . . 1471 1475 + HinfI GANTC 1471 1474 . . 1479 1483 + TfiI GAWTC 1479 1482 . . 1479 1483 + HinfI GANTC 1479 1482 . . 1491 1495 + TfiI GAWTC 1491 1494 . . 1491 1495 + HinfI GANTC 1491 1494 . . 1510 1513 + MseI TTAA 1510 1512 . . 1518 1523 + AhaIII* TTTAAA 1520 1520 . . 1519 1522 + MseI TTAA 1519 1521 . . 1538 1541 + Csp6I GTAC 1538 1540 . . 1538 1541 + RsaI GTAC 1539 1539 . . 1546 1551 + XbaI TCTAGA 1546 1550 . . 1546 1551 + Hpy178III* TCNNGA 1547 1549 . . 1547 1550 + MaeI CTAG 1547 1549 . . 1569 1574 + AhaIII* TTTAAA 1571 1571 . . 1570 1573 + MseI TTAA 1570 1572 . . 1593 1598 + BclI TGATCA 1593 1597 . . 1594 1597 + DpnI GATC 1595 1595 . . 1594 1597 + BstKTI GATC 1596 1594 . . 1594 1597 + MboI GATC 1593 1597 . . 1601 1606 + Hpy178III* TCNNGA 1602 1604 . . 1606 1611 + EcoT22I ATGCAT 1610 1606 . . 1607 1610 + CviRI* TGCA 1608 1608 . . 1608 1612 + SfaNI GCATC 1617 1621 . . 1605 1609 - SfaNI GCATC 1595 1599 . . 1619 1622 + SetI ASST 1622 1618 . . 1624 1628 + Hpy188I TCNGA 1626 1625 . . 1642 1646 + Hpy188I TCNGA 1644 1643 . . 1645 1649 - BccI CCATC 1639 1640 . . 1649 1653 - Hin4II* CCTTC 1643 1642 . . 1694 1697 + TspEI AATT 1693 1697 . . 1700 1704 + TspDTI ATGAA 1715 1713 . . 1718 1722 + DdeI CTNAG 1718 1721 . . 1718 1722 + BspCNI CTCAG 1731 1729 . . 1718 1722 + BseMII CTCAG 1732 1730 . . 1726 1729 + MnlI CCTC 1736 1735 . . 1736 1747 + BdaI TGANNNNNNTCA 1725 1723 1759 1757 1740 1743 + FatI CATG 1739 1743 . . 1740 1743 + NlaIII CATG 1743 1739 . . 1740 1743 + CviAII CATG 1740 1742 . . 1742 1747 + BclI TGATCA 1742 1746 . . 1743 1746 + DpnI GATC 1744 1744 . . 1743 1746 + BstKTI GATC 1745 1743 . . 1743 1746 + MboI GATC 1742 1746 . . 1736 1747 - BdaI TGANNNNNNTCA 1759 1757 1725 1723 1747 1752 + AclI AACGTT 1748 1750 . . 1748 1751 + TaiI ACGT 1751 1747 . . 1748 1751 + SetI ASST 1751 1747 . . 1748 1751 + MaeII ACGT 1748 1750 . . 1756 1759 - AciI CCGC 1756 1758 . . 1770 1775 + ApoI RAATTY 1770 1774 . . 1771 1774 + TspEI AATT 1770 1774 . . 1774 1778 + Hpy188I TCNGA 1776 1775 . . 1799 1802 + MseI TTAA 1799 1801 . . 1801 1804 + TspEI AATT 1800 1804 . . 1802 1809 + SwaI ATTTAAAT 1805 1805 . . 1803 1808 + AhaIII* TTTAAA 1805 1805 . . 1804 1807 + MseI TTAA 1804 1806 . . 1822 1826 + Hpy188I TCNGA 1824 1823 . . 1825 1828 - MnlI CCTC 1818 1817 . . 1838 1843 + ApoI RAATTY 1838 1842 . . 1839 1842 + TspEI AATT 1838 1842 . . 1855 1859 + TfiI GAWTC 1855 1858 . . 1855 1859 + HinfI GANTC 1855 1858 . . 1861 1864 + Csp6I GTAC 1861 1863 . . 1861 1864 + RsaI GTAC 1862 1862 . . 1863 1867 + Tsp4CI* ACNGT 1865 1864 . . 1866 1870 + Tsp45I GTSAC 1865 1870 . . 1866 1870 + MaeIII GTNAC 1865 1870 . . 1869 1873 + Tsp4CI* ACNGT 1871 1870 . . 1873 1876 + MseI TTAA 1873 1875 . . 1879 1883 + MboII GAAGA 1891 1890 . . 1883 1886 + TspEI AATT 1882 1886 . . 1911 1914 + TaiI ACGT 1914 1910 . . 1911 1914 + SetI ASST 1914 1910 . . 1911 1914 + MaeII ACGT 1911 1913 . . 1923 1928 + ApoI RAATTY 1923 1927 . . 1924 1927 + TspEI AATT 1923 1927 . . 1934 1938 + TspDTI ATGAA 1949 1947 . . 1948 1951 + MseI TTAA 1948 1950 . . 1952 1955 + TaqI TCGA 1952 1954 . . 1971 1974 + TspEI AATT 1970 1974 . . 1973 1976 + MseI TTAA 1973 1975 . . 1984 1995 + BstXI CCANNNNNNTGG 1991 1987 . . 2002 2007 + Hpy166II GTNNAC 2004 2004 . . 2002 2007 + HpaI GTTAAC 2004 2004 . . 2002 2007 + HindII GTYRAC 2004 2004 . . 2003 2006 + MseI TTAA 2003 2005 . . 2013 2017 + MboII GAAGA 2025 2024 . . 2016 2020 - BccI CCATC 2010 2011 . . 2047 2055 + AlwNI CAGNNNCTG 2052 2049 . . 2055 2059 + HinfI GANTC 2055 2058 . . 2055 2059 - PleI GAGTC 2049 2050 . . 2055 2059 - MlyI GAGTC 2049 2049 . . 2063 2066 + SetI ASST 2066 2062 . . 2063 2066 + CviJI RGCY 2064 2064 . . 2063 2066 + AluI AGCT 2064 2064 . . 2065 2068 + MaeI CTAG 2065 2067 . . 2069 2074 + SspI AATATT 2071 2071 . . 2085 2090 + VspI ATTAAT 2086 2088 . . 2086 2089 + MseI TTAA 2086 2088 . . 2088 2091 + TspEI AATT 2087 2091 . . 2091 2096 + Hpy178III* TCNNGA 2092 2094 . . 2095 2099 + TfiI GAWTC 2095 2098 . . 2095 2099 + HinfI GANTC 2095 2098 . . 2098 2102 + Hpy188I TCNGA 2100 2099 . . 2104 2109 + SfeI* CTRYAG 2104 2108 . . 2106 2110 + Tsp4CI* ACNGT 2108 2107 . . 2116 2121 + EcoRI GAATTC 2116 2120 . . 2116 2121 + ApoI RAATTY 2116 2120 . . 2117 2120 + TspEI AATT 2116 2120 . . 2120 2125 + Hpy178III* TCNNGA 2121 2123 . . 2130 2133 + TspEI AATT 2129 2133 . . 2134 2138 + BceAI ACGGC 2150 2152 . . 2149 2154 + SacI GAGCTC 2153 2149 . . 2149 2154 + Ecl136II GAGCTC 2151 2151 . . 2149 2154 + SduI GDGCHC 2153 2149 . . 2149 2154 + HgiJII* GRGCYC 2153 2149 . . 2149 2154 + HgiAI* GWGCWC 2153 2149 . . 2150 2153 + SetI ASST 2153 2149 . . 2150 2153 + CviJI RGCY 2151 2151 . . 2150 2153 + AluI AGCT 2151 2151 . . 2153 2157 - TspGWI ACGGA 2143 2141 . . 2160 2163 + TspEI AATT 2159 2163 . . 2170 2173 + TspEI AATT 2169 2173 . . 2176 2179 + Hin6I GCGC 2176 2178 . . 2176 2179 + GlaI GCGC 2177 2177 . . 2176 2179 + HhaI GCGC 2178 2176 . . 2186 2190 + Tsp4CI* ACNGT 2188 2187 . . 2203 2207 + BlsI GCNGC 2205 2204 . . 2203 2207 + BisI GCNGC 2204 2205 . . 2203 2207 + BbvI GCAGC 2215 2219 . . 2203 2207 + TseI GCWGC 2203 2206 . . 2205 2208 + CviJI RGCY 2206 2206 . . 2213 2216 + FatI CATG 2212 2216 . . 2213 2216 + NlaIII CATG 2216 2212 . . 2213 2216 + CviAII CATG 2213 2215 . . 2215 2218 + CviRI* TGCA 2216 2216 . . 2219 2222 + TaiI ACGT 2222 2218 . . 2219 2222 + SetI ASST 2222 2218 . . 2219 2222 + MaeII ACGT 2219 2221 . . 2224 2227 + TspEI AATT 2223 2227 . . 2252 2257 + Hpy166II GTNNAC 2254 2254 . . 2252 2257 + HindII GTYRAC 2254 2254 . . 2259 2262 + CviJI RGCY 2260 2260 . . 2260 2270 + MwoI GCNNNNNNNGC 2266 2263 . . 2269 2279 + MwoI GCNNNNNNNGC 2275 2272 . . 2269 2279 + BstAPI GCANNNNNTGC 2275 2272 . . 2277 2280 + CviRI* TGCA 2278 2278 . . 2289 2292 + TspEI AATT 2288 2292 . . 2294 2297 + TaqI TCGA 2294 2296 . . 2296 2300 + PleI GAGTC 2304 2305 . . 2296 2300 + MlyI GAGTC 2305 2305 . . 2296 2300 + HinfI GANTC 2296 2299 . . 2304 2308 + FokI GGATG 2317 2321 . . 2304 2308 + BseGI GGATG 2310 2308 . . 2309 2313 - TspDTI ATGAA 2299 2297 . . 2317 2320 + Csp6I GTAC 2317 2319 . . 2317 2320 + RsaI GTAC 2318 2318 . . 2326 2331 + EcoRV GATATC 2328 2328 . . 2330 2335 + Hpy178III* TCNNGA 2331 2333 . . 2330 2335 + BspHI TCATGA 2330 2334 . . 2331 2334 + FatI CATG 2330 2334 . . 2331 2334 + NlaIII CATG 2334 2330 . . 2331 2334 + CviAII CATG 2331 2333 . . 2337 2341 - BslFI GGGAC 2322 2326 . . 2341 2351 + BsiYI* CCNNNNNNNGG 2347 2344 . . 2348 2352 + Tsp4CI* ACNGT 2350 2349 . . 2359 2364 + SspI AATATT 2361 2361 . . 2363 2368 + PsiI TTATAA 2365 2365 . . 2370 2375 + NlaIV GGNNCC 2372 2372 . . 2371 2375 + BmgT120I GGNCC 2372 2373 . . 2371 2375 + AsuI* GGNCC 2371 2374 . . 2372 2375 + CviJI RGCY 2373 2373 . . 2372 2375 + HaeIII GGCC 2373 2373 . . 2374 2384 + BsiYI* CCNNNNNNNGG 2380 2377 . . 2381 2386 - TaqII CACCCA 2371 2369 . . 2414 2420 + TspRI CASTGNN 2420 2411 . . 2414 2419 - BtsI GCAGTG 2413 2411 . . 2436 2439 + DpnI GATC 2437 2437 . . 2436 2439 + BstKTI GATC 2438 2436 . . 2436 2439 + MboI GATC 2435 2439 . . 2440 2443 + CviJI RGCY 2441 2441 . . 2452 2456 + FauI CCCGC 2460 2462 . . 2449 2453 - BslFI GGGAC 2434 2438 . . 2453 2456 + AciI CCGC 2453 2455 . . 2458 2462 + MboII GAAGA 2470 2469 . . 2463 2466 + DpnI GATC 2464 2464 . . 2463 2466 + BstKTI GATC 2465 2463 . . 2458 2463 - Ksp632I* CTCTTC 2453 2456 . . 2463 2466 + MboI GATC 2462 2466 . . 2465 2469 + Hpy188I TCNGA 2467 2466 . . 2470 2473 + MseI TTAA 2470 2472 . . 2486 2489 + TaiI ACGT 2489 2485 . . 2486 2489 + SetI ASST 2489 2485 . . 2486 2489 + MaeII ACGT 2486 2488 . . 2488 2491 + Csp6I GTAC 2488 2490 . . 2488 2491 + RsaI GTAC 2489 2489 . . 2496 2499 + TaqI TCGA 2496 2498 . . 2498 2502 + TfiI GAWTC 2498 2501 . . 2498 2502 + HinfI GANTC 2498 2501 . . 2504 2511 + FspAI RTGCGCAY 2507 2507 . . 2505 2510 + MstI* TGCGCA 2507 2507 . . 2506 2509 + Hin6I GCGC 2506 2508 . . 2506 2509 + GlaI GCGC 2507 2507 . . 2506 2509 + HhaI GCGC 2508 2506 . . 2519 2524 + PsiI TTATAA 2521 2521 . . 2525 2529 + Tsp4CI* ACNGT 2527 2526 . . 2527 2531 + HphI GGTGA 2539 2538 . . 2530 2534 + TfiI GAWTC 2530 2533 . . 2530 2534 + HinfI GANTC 2530 2533 . . 2539 2544 + AhaIII* TTTAAA 2541 2541 . . 2540 2543 + MseI TTAA 2540 2542 . . 2557 2562 + EcoRV GATATC 2559 2559 . . 2570 2575 + Hpy178III* TCNNGA 2571 2573 . . 2578 2581 + TspEI AATT 2577 2581 . . 2581 2584 + CviRI* TGCA 2582 2582 . . 2589 2593 + Tsp4CI* ACNGT 2591 2590 . . 2618 2622 + MboII GAAGA 2630 2629 . . 2622 2625 + SetI ASST 2625 2621 . . 2618 2623 - Ksp632I* CTCTTC 2613 2616 . . 2621 2624 - MnlI CCTC 2614 2613 . . 2624 2627 + Csp6I GTAC 2624 2626 . . 2624 2627 + RsaI GTAC 2625 2625 . . 2626 2630 + BsrI ACTGG 2631 2629 . . 2630 2635 + Cac8I GCNNGC 2632 2632 . . 2638 2642 - BccI CCATC 2632 2633 . . 2649 2652 + TspEI AATT 2648 2652 . . 2662 2665 + MseI TTAA 2662 2664 . . 2677 2681 + Hpy188I TCNGA 2679 2678 . . 2678 2682 + DdeI CTNAG 2678 2681 . . 2678 2682 - BspCNI CTCAG 2670 2668 . . 2678 2682 - BseMII CTCAG 2669 2667 . . 2683 2686 + MseI TTAA 2683 2685 . . 2687 2691 + Tsp45I GTSAC 2686 2691 . . 2687 2691 + MaeIII GTNAC 2686 2691 . . 2722 2732 + BsiYI* CCNNNNNNNGG 2728 2725 . . 2724 2727 + FatI CATG 2723 2727 . . 2724 2727 + NlaIII CATG 2727 2723 . . 2724 2727 + CviAII CATG 2724 2726 . . 2727 2732 - GsaI CCCAGC 2731 2727 . . 2727 2732 - BseYI CCCAGC 2727 2731 . . 2745 2749 - TspGWI ACGGA 2735 2733 . . 2749 2752 + MseI TTAA 2749 2751 . . 2758 2761 + TspEI AATT 2757 2761 . . 2764 2769 + NdeI CATATG 2765 2767 . . 2773 2778 + BsaAI YACGTR 2775 2775 . . 2774 2777 + TaiI ACGT 2777 2773 . . 2774 2777 + SetI ASST 2777 2773 . . 2774 2777 + MaeII ACGT 2774 2776 . . 2779 2783 + MboII GAAGA 2791 2790 . . 2791 2795 + BsrI ACTGG 2796 2794 . . 2797 2800 - MnlI CCTC 2790 2789 . . 2801 2805 + BsrI ACTGG 2806 2804 . . 2804 2808 + FokI GGATG 2817 2821 . . 2804 2808 + BseGI GGATG 2810 2808 . . 2814 2817 + TspEI AATT 2813 2817 . . 2830 2833 + MseI TTAA 2830 2832 . . 2847 2852 + PsiI TTATAA 2849 2849 . . 2861 2865 + FokI GGATG 2874 2878 . . 2861 2865 + BseGI GGATG 2867 2865 . . 2863 2867 + TspDTI ATGAA 2878 2876 . . 2872 2882 + MwoI GCNNNNNNNGC 2878 2875 . . 2876 2879 + TspEI AATT 2875 2879 . . 2881 2886 + Cac8I GCNNGC 2883 2883 . . 2886 2890 + BssKI CCNGG 2885 2890 . . 2886 2890 + BseBI CCWGG 2887 2888 . . 2886 2890 + ScrFI CCNGG 2887 2888 . . 2886 2890 + EcoRII CCWGG 2885 2890 . . 2900 2905 + PsiI TTATAA 2902 2902 . . 2911 2916 - BsmI GAATGC 2911 2909 . . 2923 2927 + Hpy188I TCNGA 2925 2924 . . 2926 2930 + MboII GAAGA 2938 2937 . . 2930 2933 + TspEI AATT 2929 2933 . . 2934 2937 - AciI CCGC 2934 2936 . . 2941 2945 + TfiI GAWTC 2941 2944 . . 2937 2941 - Hin4II* CCTTC 2931 2930 . . 2941 2945 + HinfI GANTC 2941 2944 . . 2948 2951 + MseI TTAA 2948 2950 . . 2953 2957 + MboII GAAGA 2965 2964 . . 2953 2958 + BbvII* GAAGAC 2960 2964 . . 2956 2960 + HgaI GACGC 2965 2970 . . 2957 2962 + MluI ACGCGT 2957 2961 . . 2957 2962 + AflIII ACRYGT 2957 2961 . . 2958 2961 + FnuDII* CGCG 2959 2959 . . 2963 2974 + BdaI TGANNNNNNTCA 2952 2950 2986 2984 2964 2973 + XmnI GAANNNNTTC 2968 2968 . . 2966 2969 + TspEI AATT 2965 2969 . . 2974 2977 + SetI ASST 2977 2973 . . 2974 2977 + CviJI RGCY 2975 2975 . . 2963 2974 - BdaI TGANNNNNNTCA 2986 2984 2952 2950 2974 2977 + AluI AGCT 2975 2975 . . 2988 2993 + SspI AATATT 2990 2990 . . 2992 2995 + MseI TTAA 2992 2994 . . 3016 3020 + TspGWI ACGGA 3031 3029 . . 3026 3031 + Hpy178III* TCNNGA 3027 3029 . . 3026 3031 + NruI TCGCGA 3028 3028 . . 3027 3030 + FnuDII* CGCG 3028 3028 . . 3032 3035 + TspEI AATT 3031 3035 . . 3046 3051 + EcoRV GATATC 3048 3048 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 1191 1866 # # AciI: # [CCGC] # 4 36 42 123 340 481 # 697 1334 # # AclI: # [AACGTT] # 623 1125 1309 # # AflIII: # [ACRYGT] # 100 1023 1934 # # AhaIII*: # [TTTAAA] # 51 234 243 516 621 656 # 736 # # AjuI: # [GAANNNNNNNTTGG] # 32 68 2957 # # AluI: # [AGCT] # 54 82 87 125 587 824 # 1298 # # AlwNI: # [CAGNNNCTG] # 1005 2052 # # ApoI: # [RAATTY] # 9 68 85 94 108 193 # 210 643 706 941 # # AsuI*: # [GGNCC] # 420 686 912 1039 # # AsuII: # [TTCGAA] # 214 2843 # # AvaII: # [GGWCC] # 420 912 1725 # # BbvI: # [GCAGC] # 842 2215 # # BbvII*: # [GAAGAC] # 97 2960 # # BccI: # [CCATC] # 118 345 371 425 622 1176 # # Bce83I*: # [CTTGAG] # 551 2506 # # BceAI: # [ACGGC] # 476 649 907 1025 # # BclI: # [TGATCA] # 149 1315 1593 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 0 0 # 0 0 34 34 34 34 # 71 239 580 838 1193 # # BinI*: # [GGATC] # 555 700 1802 # # BisI: # [GCNGC] # 787 853 1417 # # BlsI: # [GCNGC] # 787 852 1418 # # BmgT120I: # [GGNCC] # 421 685 912 1039 # # BsaAI: # [YACGTR] # 282 2775 # # BsaBI: # [GATNNNNATC] # 392 575 2090 # # BseBI: # [CCWGG] # 170 174 536 2177 # # BseGI: # [GGATG] # 57 117 190 498 500 826 # 869 # # BseMII: # [CTCAG] # 388 937 1732 # # BseYI: # [CCCAGC] # 330 2727 # # BsiYI*: # [CCNNNNNNNGG] # 33 190 329 348 2157 # # BslFI: # [GGGAC] # 20 112 343 465 623 1494 # # BsmAI: # [GTCTC] # 518 630 1909 # # BsmI: # [GAATGC] # 146 2911 # # Bsp1407I: # [TGTACA] # 1398 1659 # # BspCNI: # [CTCAG] # 387 939 1731 # # BspHI: # [TCATGA] # 727 2330 # # BspLU11I*: # [ACATGT] # 1023 2034 # # BspMI: # [ACCTGC] # 14 1177 1866 # # BsrBI: # [CCGCTC] # 1416 1641 # # BsrI: # [ACTGG] # 10 165 251 1001 1630 # # BssKI: # [CCNGG] # 71 103 172 534 2177 # # BstAPI: # [GCANNNNNTGC] # 493 782 1782 # # BstKTI: # [GATC] # 27 78 149 171 306 333 # 592 693 708 # # BstXI: # [CCANNNNNNTGG] # 688 1066 1303 # # BtsI: # [GCAGTG] # 201 644 2212 # # Cac8I: # [GCNNGC] # 40 156 174 251 521 1915 # # CauII*: # [CCSGG] # 607 2450 # # ClaI: # [ATCGAT] # 551 2506 # # Csp6I: # [GTAC] # 120 136 139 141 171 194 # 211 235 239 259 323 433 # 456 # # CviAII: # [CATG] # 28 41 118 204 321 333 # 393 443 473 703 # # CviJI: # [RGCY] # 33 48 48 54 54 55 # 65 68 72 82 87 97 # 113 125 143 299 435 534 # 645 # # CviRI*: # [TGCA] # 9 55 62 70 79 154 # 161 172 278 304 475 608 # 630 # # DdeI: # [CTNAG] # 8 272 359 379 524 555 # 960 # # DpnI: # [GATC] # 27 78 149 171 306 333 # 593 693 707 # # DraII: # [RGGNCCY] # 420 2637 # # DsaI*: # [CCRYGG] # 665 2392 # # Ecl136II: # [GAGCTC] # 906 2151 # # Eco47III: # [AGCGCT] # 690 2367 # # Eco57I: # [CTGAAG] # 344 2713 # # Eco57MI: # [CTGRAG] # 344 2713 # # EcoRI: # [GAATTC] # 210 941 1906 # # EcoRII: # [CCWGG] # 172 174 534 2177 # # EcoRV: # [GATATC] # 9 231 489 2328 # # EcoT22I: # [ATGCAT] # 434 1176 1447 # # Esp3I: # [CGTCTC] # 1148 1909 # # FalI: # [AAGNNNNNCTT] # 0 0 32 1403 1622 # # FatI: # [CATG] # 28 41 118 204 321 334 # 393 443 473 702 # # FauI: # [CCCGC] # 597 2460 # # FnuDII*: # [CGCG] # 29 69 2959 # # FokI: # [GGATG] # 57 117 183 500 505 806 # 889 # # FspAI: # [RTGCGCAY] # 550 2507 # # GlaI: # [GCGC] # 96 330 550 594 1487 # # GsaI: # [CCCAGC] # 326 2731 # # HaeII: # [RGCGCY] # 98 594 2365 # # HaeIII: # [GGCC] # 684 2373 # # HgaI: # [GACGC] # 66 92 2899 # # HgiAI*: # [GWGCWC] # 904 2153 # # HgiCI*: # [GGYRCC] # 712 2345 # # HgiJII*: # [GRGCYC] # 904 2153 # # HhaI: # [GCGC] # 97 330 549 594 1487 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 127 1093 # 1773 # # Hin4II*: # [CCTTC] # 16 30 126 381 401 815 # 1288 # # Hin6I: # [GCGC] # 95 330 551 594 1487 # # HindII: # [GTYRAC] # 250 803 2004 # # HinfI: # [GANTC] # 8 12 32 40 94 106 # 116 165 200 201 202 244 # 364 411 412 450 # # HpaI: # [GTTAAC] # 1053 2004 # # HpaII: # [CCGG] # 607 2450 # # HphI: # [GGTGA] # 71 193 210 355 386 518 # 1324 # # Hpy166II: # [GTNNAC] # 250 603 627 774 803 # # Hpy178III*: # [TCNNGA] # 29 30 33 55 210 240 # 456 490 535 979 # # Hpy188I: # [TCNGA] # 18 48 51 132 132 174 # 180 212 246 276 367 391 # 830 # # Ksp632I*: # [CTCTTC] # 125 160 207 444 480 594 # 1047 # # MaeI: # [CTAG] # 233 398 518 916 992 # # MaeII: # [ACGT] # 44 163 184 267 283 288 # 308 623 897 # # MaeIII: # [GTNAC] # 165 333 349 371 406 612 # 821 # # MboI: # [GATC] # 27 78 149 171 306 333 # 595 693 705 # # MboII: # [GAAGA] # 5 12 12 27 52 63 # 92 93 134 137 143 147 # 160 161 324 445 468 582 # # MluI: # [ACGCGT] # 100 2957 # # MlyI: # [GAGTC] # 256 565 752 1484 # # MmeI: # [TCCRAC] # 66 2991 # # MnlI: # [CCTC] # 6 6 15 82 92 126 # 144 176 186 267 796 1161 # # MseI: # [TTAA] # 5 9 15 21 25 30 # 36 40 44 51 65 66 # 69 70 75 81 83 84 # 104 118 122 153 162 229 # 254 297 365 384 # # MslI: # [CAYNNNNRTG] # 1301 1756 # # MstI*: # [TGCGCA] # 550 2507 # # MwoI: # [GCNNNNNNNGC] # 9 101 179 232 392 603 # 1541 # # NdeI: # [CATATG] # 72 292 1178 1515 # # NlaIII: # [CATG] # 28 41 118 204 321 330 # 393 443 473 706 # # NlaIV: # [GGNNCC] # 685 714 1658 # # NruI: # [TCGCGA] # 29 3028 # # NspI: # [RCATGY] # 1027 2030 # # PfoI: # [TCCNGGA] # 605 2452 # # PleI: # [GAGTC] # 255 564 753 1485 # # PpuMI: # [RGGWCCY] # 420 2637 # # PsiI: # [TTATAA] # 53 155 156 328 2365 # # RsaI: # [GTAC] # 120 136 139 141 171 194 # 211 235 240 259 323 432 # 456 # # SacI: # [GAGCTC] # 904 2153 # # SapI: # [GCTCTTC] # 480 926 1651 # # SauI*: # [CCTNAGG] # 796 2261 # # ScaI: # [AGTACT] # 995 2062 # # ScrFI: # [CCNGG] # 71 103 170 536 2177 # # SduI: # [GDGCHC] # 904 2153 # # SecI*: # [CCNNGG] # 264 401 2392 # # SetI: # [ASST] # 6 9 15 20 22 28 # 28 30 44 52 54 69 # 75 80 87 109 125 129 # 136 152 152 163 200 201 # 233 235 267 336 # # SfaNI: # [GCATC] # 22 42 167 171 182 441 # 592 1440 # # SfeI*: # [CTRYAG] # 383 575 953 1146 # # SmlI: # [CTYRAG] # 566 2491 # # SpeI: # [ACTAGT] # 232 2825 # # SspI: # [AATATT] # 67 99 290 629 877 1095 # # StyI: # [CCWWGG] # 401 2656 # # SwaI: # [ATTTAAAT] # 899 906 1252 # # TaiI: # [ACGT] # 44 163 184 267 280 288 # 308 626 897 # # TaqI: # [TCGA] # 30 202 214 307 342 561 # 1401 # # TaqII: # [CACCCA] # 686 2371 # # TatI: # [WGTACW] # 194 211 473 520 1659 # # TauI: # [GCSGC] # 1419 1638 # # TfiI: # [GAWTC] # 8 12 32 94 106 116 # 165 240 244 364 403 411 # 862 # # TseI: # [GCWGC] # 854 2203 # # TsoI: # [TARCCA] # 455 859 1743 # # Tsp45I: # [GTSAC] # 371 821 847 1018 # # Tsp4CI*: # [ACNGT] # 6 64 80 94 162 165 # 177 231 237 339 406 466 # 630 # # TspDTI: # [ATGAA] # 16 92 102 143 164 179 # 234 350 579 583 615 # # TspEI: # [AATT] # 9 10 10 13 26 29 # 30 30 31 36 38 41 # 44 47 54 54 56 58 # 62 65 66 71 72 77 # 94 104 108 109 110 117 # 129 138 264 279 287 289 # # TspGWI: # [ACGGA] # 26 296 592 2143 # # TspRI: # [CASTGNN] # 72 129 637 2219 # # VspI: # [ATTAAT] # 971 2086 # # XbaI: # [TCTAGA] # 1511 1546 # # XcmI: # [CCANNNNNNNNNTGG] # 993 2064 # # XmnI: # [GAANNNNTTC] # 89 292 340 744 1592 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3057 # Reported_sequences: 1 # Reported_hitcount: 622 #---------------------------------------