Restriction Fragments for PAT1/YCR077C



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The currently selected gene/sequence is :

PAT1/YCR077C on chromosome III from coordinates 252628 to 250238.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.26972.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 9 + Hin4II* CCTTC 15 14 . . 5 15 + BsiYI* CCNNNNNNNGG 11 8 . . 26 29 - AciI CCGC 26 28 . . 34 37 + Hin6I GCGC 34 36 . . 34 37 + GlaI GCGC 35 35 . . 34 37 + HhaI GCGC 36 34 . . 35 38 + FnuDII* CGCG 36 36 . . 39 43 + FokI GGATG 52 56 . . 39 43 + BseGI GGATG 45 43 . . 36 39 - AciI CCGC 36 38 . . 36 40 - FauI CCCGC 29 31 . . 43 47 + BmgT120I GGNCC 44 45 . . 43 47 + AvaII GGWCC 43 46 . . 43 47 + AsuI* GGNCC 43 46 . . 40 44 - BccI CCATC 34 35 . . 46 49 + MnlI CCTC 56 55 . . 48 53 + Hpy178III* TCNNGA 49 51 . . 58 62 + MboII GAAGA 70 69 . . 58 63 - Ksp632I* CTCTTC 53 56 . . 65 69 + MaeIII GTNAC 64 69 . . 73 76 + CviJI RGCY 74 74 . . 80 85 + Cac8I GCNNGC 82 82 . . 82 87 + SduI GDGCHC 86 82 . . 82 87 + HgiAI* GWGCWC 86 82 . . 91 96 + SmlI CTYRAG 91 95 . . 91 96 + Bce83I* CTTGAG 112 110 . . 94 99 + BseRI GAGGAG 109 107 . . 94 97 - MnlI CCTC 87 86 . . 114 118 + Hpy99I CGWCG 118 113 . . 127 131 + HphI GGTGA 139 138 . . 129 140 + BdaI TGANNNNNNTCA 118 116 152 150 140 143 + SetI ASST 143 139 . . 129 140 - BdaI TGANNNNNNTCA 152 150 118 116 145 150 + KpnI GGTACC 149 145 . . 145 150 + NlaIV GGNNCC 147 147 . . 145 150 + HgiCI* GGYRCC 145 149 . . 145 150 + Acc65I GGTACC 145 149 . . 146 149 + Csp6I GTAC 146 148 . . 146 149 + RsaI GTAC 147 147 . . 169 172 + MnlI CCTC 179 178 . . 174 179 + EcoP15I CAGCAG 204 206 . . 176 186 + MwoI GCNNNNNNNGC 182 179 . . 176 180 + BlsI GCNGC 178 177 . . 176 180 + BisI GCNGC 177 178 . . 176 180 + BbvI GCAGC 188 192 . . 176 180 + TseI GCWGC 176 179 . . 177 182 + NspBII* CMGCKG 179 179 . . 179 183 + TauI GCSGC 182 179 . . 179 189 + MwoI GCNNNNNNNGC 185 182 . . 179 183 + BlsI GCNGC 181 180 . . 179 183 + BisI GCNGC 180 181 . . 182 192 + MwoI GCNNNNNNNGC 188 185 . . 182 186 + BlsI GCNGC 184 183 . . 182 186 + BisI GCNGC 183 184 . . 182 186 + BbvI GCAGC 194 198 . . 182 186 + TseI GCWGC 182 185 . . 179 182 - AciI CCGC 179 181 . . 183 188 + EcoP15I CAGCAG 213 215 . . 185 189 + BlsI GCNGC 187 186 . . 185 189 + BisI GCNGC 186 187 . . 185 189 + BbvI GCAGC 197 201 . . 185 189 + TseI GCWGC 185 188 . . 186 191 + NspBII* CMGCKG 188 188 . . 188 192 + TauI GCSGC 191 188 . . 188 192 + BlsI GCNGC 190 189 . . 188 192 + BisI GCNGC 189 190 . . 188 191 - AciI CCGC 188 190 . . 197 202 + MfeI CAATTG 197 201 . . 198 201 + TspEI AATT 197 201 . . 211 216 + AcyI GRCGYC 212 214 . . 213 217 + Hpy99I CGWCG 217 212 . . 212 216 - HgaI GACGC 201 206 . . 217 222 + NlaIV GGNNCC 219 219 . . 217 222 + HgiCI* GGYRCC 217 221 . . 221 226 + DsaI* CCRYGG 221 225 . . 221 226 + SecI* CCNNGG 221 225 . . 223 227 + BceAI ACGGC 239 241 . . 225 228 + CviJI RGCY 226 226 . . 227 230 + MaeI CTAG 227 229 . . 230 233 + DpnI GATC 231 231 . . 230 233 + BstKTI GATC 232 230 . . 230 233 + MboI GATC 229 233 . . 233 238 + NdeI CATATG 234 236 . . 240 243 + CviRI* TGCA 241 241 . . 241 251 + MwoI GCNNNNNNNGC 247 244 . . 241 251 + BstAPI GCANNNNNTGC 247 244 . . 241 245 + BlsI GCNGC 243 242 . . 241 245 + BisI GCNGC 242 243 . . 241 245 + BbvI GCAGC 253 257 . . 241 245 + TseI GCWGC 241 244 . . 242 250 + AlwNI CAGNNNCTG 247 244 . . 243 246 + SetI ASST 246 242 . . 243 246 + CviJI RGCY 244 244 . . 243 246 + AluI AGCT 244 244 . . 248 253 + PstI CTGCAG 252 248 . . 248 253 + SfeI* CTRYAG 248 252 . . 249 252 + CviRI* TGCA 250 250 . . 253 257 - Hin4II* CCTTC 247 246 . . 258 261 + TspEI AATT 257 261 . . 263 267 + TauI GCSGC 266 263 . . 263 267 + BlsI GCNGC 265 264 . . 263 267 + BisI GCNGC 264 265 . . 265 268 + CviJI RGCY 266 266 . . 265 268 + HaeIII GGCC 266 266 . . 265 269 + BmgT120I GGNCC 266 267 . . 265 269 + AsuI* GGNCC 265 268 . . 263 266 - AciI CCGC 263 265 . . 268 273 + StyI CCWWGG 268 272 . . 268 273 + SecI* CCNNGG 268 272 . . 268 273 + AvrII CCTAGG 268 272 . . 269 272 + MaeI CTAG 269 271 . . 272 276 + BmgT120I GGNCC 273 274 . . 272 276 + AvaII GGWCC 272 275 . . 272 276 + AsuI* GGNCC 272 275 . . 273 278 + TaqII GACCGA 289 287 . . 277 281 - BccI CCATC 271 272 . . 283 287 + BceAI ACGGC 299 301 . . 286 290 + BlsI GCNGC 288 287 . . 286 290 + BisI GCNGC 287 288 . . 286 290 + BbvI GCAGC 298 302 . . 286 290 + TseI GCWGC 286 289 . . 287 292 + EcoP15I CAGCAG 317 319 . . 289 293 + BlsI GCNGC 291 290 . . 289 293 + BisI GCNGC 290 291 . . 289 293 + BbvI GCAGC 301 305 . . 289 293 + TseI GCWGC 289 292 . . 290 295 + EcoP15I CAGCAG 320 322 . . 294 300 + PpuMI RGGWCCY 295 298 . . 294 300 + DraII RGGNCCY 295 298 . . 295 299 + BmgT120I GGNCC 296 297 . . 295 299 + AvaII GGWCC 295 298 . . 295 299 + AsuI* GGNCC 295 298 . . 297 300 + SetI ASST 300 296 . . 298 301 + MnlI CCTC 308 307 . . 300 305 + XbaI TCTAGA 300 304 . . 300 305 + Hpy178III* TCNNGA 301 303 . . 301 304 + MaeI CTAG 301 303 . . 305 308 + SetI ASST 308 304 . . 307 312 + Eco57MI CTGRAG 328 326 . . 307 312 + Eco57I CTGAAG 328 326 . . 311 314 + CviJI RGCY 312 312 . . 319 323 + TfiI GAWTC 319 322 . . 319 323 + HinfI GANTC 319 322 . . 318 329 - TstI CACNNNNNNTCC 342 337 310 305 330 335 + Hpy166II GTNNAC 332 332 . . 330 335 + AccI GTMKAC 331 333 . . 336 339 + CviRI* TGCA 337 337 . . 335 340 - BsgI GTGCAG 320 318 . . 342 345 + SetI ASST 345 341 . . 343 346 + MnlI CCTC 353 352 . . 344 349 - Eco57MI CTGRAG 329 327 . . 349 354 + BsrDI GCAATG 356 354 . . 344 349 - GsuI CTGGAG 329 327 . . 354 359 + NarI GGCGCC 355 357 . . 354 359 + KasI GGCGCC 354 358 . . 354 359 + DinI GGCGCC 356 356 . . 354 359 + HaeII RGCGCY 358 354 . . 354 359 + NlaIV GGNNCC 356 356 . . 354 359 + AcyI GRCGYC 355 357 . . 354 359 + HgiCI* GGYRCC 354 358 . . 355 358 + Hin6I GCGC 355 357 . . 355 358 + GlaI GCGC 356 356 . . 355 358 + HhaI GCGC 357 355 . . 358 362 + Hin4II* CCTTC 368 367 . . 361 365 - HphI GGTGA 353 352 . . 370 375 + TatI WGTACW 370 374 . . 371 374 + Csp6I GTAC 371 373 . . 371 374 + RsaI GTAC 372 372 . . 373 383 + BsaXI ACNNNNNCTCC 363 360 393 390 378 381 + CviJI RGCY 379 379 . . 378 383 + NlaIV GGNNCC 380 380 . . 382 385 + HpaII CCGG 382 384 . . 384 387 + CviJI RGCY 385 385 . . 384 389 + NlaIV GGNNCC 386 386 . . 394 397 + AciI CCGC 394 396 . . 396 400 + BlsI GCNGC 398 397 . . 396 400 + BisI GCNGC 397 398 . . 396 400 + BbvI GCAGC 408 412 . . 396 400 + TseI GCWGC 396 399 . . 405 408 + CviJI RGCY 406 406 . . 405 408 + HaeIII GGCC 406 406 . . 405 410 + NlaIV GGNNCC 407 407 . . 405 409 + BmgT120I GGNCC 406 407 . . 405 409 + AsuI* GGNCC 405 408 . . 412 417 + SfeI* CTRYAG 412 416 . . 416 419 + CviJI RGCY 417 417 . . 427 430 + TaqI TCGA 427 429 . . 431 434 + CviRI* TGCA 432 432 . . 429 433 - SfaNI GCATC 419 423 . . 446 457 + DrdI GACNNNNNNGTC 452 450 . . 453 456 + TaiI ACGT 456 452 . . 453 456 + SetI ASST 456 452 . . 453 456 + MaeII ACGT 453 455 . . 461 464 + CviRI* TGCA 462 462 . . 462 466 + BlsI GCNGC 464 463 . . 462 466 + BisI GCNGC 463 464 . . 462 466 + BbvI GCAGC 474 478 . . 462 466 + TseI GCWGC 462 465 . . 468 472 + Tsp4CI* ACNGT 470 469 . . 475 479 + TspDTI ATGAA 490 488 . . 477 482 + ApoI RAATTY 477 481 . . 478 481 + TspEI AATT 477 481 . . 483 493 + BsiYI* CCNNNNNNNGG 489 486 . . 483 493 + PflMI CCANNNNNTGG 489 486 . . 488 493 + DsaI* CCRYGG 488 492 . . 488 493 + StyI CCWWGG 488 492 . . 488 493 + NcoI CCATGG 488 492 . . 488 493 + SecI* CCNNGG 488 492 . . 489 492 + FatI CATG 488 492 . . 489 492 + NlaIII CATG 492 488 . . 489 492 + CviAII CATG 489 491 . . 500 511 + TstI CACNNNNNNTCC 491 486 523 518 496 500 - FokI GGATG 482 486 . . 496 500 - BseGI GGATG 495 493 . . 501 511 + BsaXI ACNNNNNCTCC 491 488 521 518 505 510 + Eco31I GGTCTC 511 515 . . 506 510 + BsmAI GTCTC 511 515 . . 516 521 + NlaIV GGNNCC 518 518 . . 517 521 + BmgT120I GGNCC 518 519 . . 517 521 + AvaII GGWCC 517 520 . . 517 521 + AsuI* GGNCC 517 520 . . 520 523 + AciI CCGC 520 522 . . 522 532 + MwoI GCNNNNNNNGC 528 525 . . 523 526 + MnlI CCTC 533 532 . . 523 529 + Bpu10I CCTNAGC 524 527 . . 523 529 + BbvCI CCTCAGC 524 527 . . 519 524 - EciI GGCGGA 509 507 . . 524 528 + DdeI CTNAG 524 527 . . 524 528 + BspCNI CTCAG 537 535 . . 524 528 + BseMII CTCAG 538 536 . . 526 531 + EcoP15I CAGCAG 556 558 . . 528 532 + BlsI GCNGC 530 529 . . 528 532 + BisI GCNGC 529 530 . . 528 532 + BbvI GCAGC 540 544 . . 528 532 + TseI GCWGC 528 531 . . 533 536 + TspEI AATT 532 536 . . 542 545 + CviRI* TGCA 543 543 . . 543 547 + BlsI GCNGC 545 544 . . 543 547 + BisI GCNGC 544 545 . . 543 547 + BbvI GCAGC 555 559 . . 543 547 + TseI GCWGC 543 546 . . 545 548 + CviJI RGCY 546 546 . . 546 551 + Cac8I GCNNGC 548 548 . . 547 557 + BsiYI* CCNNNNNNNGG 553 550 . . 551 555 + Hpy99I CGWCG 555 550 . . 550 554 - HgaI GACGC 539 544 . . 557 562 + Hpy166II GTNNAC 559 559 . . 557 562 + HindII GTYRAC 559 559 . . 561 567 + SexAI ACCWGGT 561 566 . . 562 566 + BssKI CCNGG 561 566 . . 562 566 + BseBI CCWGG 563 564 . . 562 566 + ScrFI CCNGG 563 564 . . 562 566 + EcoRII CCWGG 561 566 . . 564 567 + SetI ASST 567 563 . . 564 570 + PpuMI RGGWCCY 565 568 . . 564 570 + DraII RGGNCCY 565 568 . . 565 570 + NlaIV GGNNCC 567 567 . . 565 569 + BmgT120I GGNCC 566 567 . . 565 569 + AvaII GGWCC 565 568 . . 565 569 + AsuI* GGNCC 565 568 . . 569 572 + MnlI CCTC 579 578 . . 566 570 - BslFI GGGAC 551 555 . . 575 578 + TspEI AATT 574 578 . . 580 583 + Hin6I GCGC 580 582 . . 580 583 + GlaI GCGC 581 581 . . 580 583 + HhaI GCGC 582 580 . . 583 586 + MnlI CCTC 593 592 . . 588 591 + SetI ASST 591 587 . . 589 592 + MnlI CCTC 599 598 . . 592 602 + BsiYI* CCNNNNNNNGG 598 595 . . 592 602 + PflMI CCANNNNNTGG 598 595 . . 594 597 + SetI ASST 597 593 . . 595 598 + MnlI CCTC 605 604 . . 598 602 + BssKI CCNGG 597 602 . . 598 602 + BseBI CCWGG 599 600 . . 598 602 + ScrFI CCNGG 599 600 . . 598 602 + EcoRII CCWGG 597 602 . . 605 608 + MseI TTAA 605 607 . . 616 620 + TspDTI ATGAA 631 629 . . 618 622 + TfiI GAWTC 618 621 . . 618 622 + HinfI GANTC 618 621 . . 633 639 + PpuMI RGGWCCY 634 637 . . 633 639 + DraII RGGNCCY 634 637 . . 633 638 + NlaIV GGNNCC 635 635 . . 634 638 + BmgT120I GGNCC 635 636 . . 634 638 + AvaII GGWCC 634 637 . . 634 638 + AsuI* GGNCC 634 637 . . 639 644 + TatI WGTACW 639 643 . . 639 644 + Bsp1407I TGTACA 639 643 . . 640 643 + Csp6I GTAC 640 642 . . 640 643 + RsaI GTAC 641 641 . . 649 653 - BsrI ACTGG 649 647 . . 656 661 + HindIII AAGCTT 656 660 . . 657 660 + SetI ASST 660 656 . . 657 660 + CviJI RGCY 658 658 . . 657 660 + AluI AGCT 658 658 . . 664 668 + Hin4II* CCTTC 674 673 . . 668 673 + TaqII CACCCA 684 682 . . 671 675 + BccI CCATC 679 680 . . 667 671 - HphI GGTGA 659 658 . . 672 681 + MslI CAYNNNNRTG 676 676 . . 682 687 + MmeI TCCRAC 707 705 . . 687 698 + TstI CACNNNNNNTCC 678 673 710 705 688 698 + BsaXI ACNNNNNCTCC 678 675 708 705 691 695 + Hin4II* CCTTC 701 700 . . 691 701 + EcoNI CCTNNNNNAGG 695 696 . . 691 701 + BsiYI* CCNNNNNNNGG 697 694 . . 697 701 + BssKI CCNGG 696 701 . . 697 701 + BseBI CCWGG 698 699 . . 697 701 + ScrFI CCNGG 698 699 . . 697 701 + EcoRII CCWGG 696 701 . . 698 706 + AlwNI CAGNNNCTG 703 700 . . 699 705 + DraII RGGNCCY 700 703 . . 695 700 - Eco57MI CTGRAG 680 678 . . 700 703 + CviJI RGCY 701 701 . . 700 703 + HaeIII GGCC 701 701 . . 695 700 - GsuI CTGGAG 680 678 . . 700 704 + BmgT120I GGNCC 701 702 . . 700 704 + AsuI* GGNCC 700 703 . . 703 713 + BsiYI* CCNNNNNNNGG 709 706 . . 713 716 + Hin6I GCGC 713 715 . . 713 716 + GlaI GCGC 714 714 . . 713 716 + HhaI GCGC 715 713 . . 727 730 + MnlI CCTC 737 736 . . 731 734 + CviRI* TGCA 732 732 . . 737 742 + Hpy166II GTNNAC 739 739 . . 740 744 + HgaI GACGC 749 754 . . 746 749 + DpnI GATC 747 747 . . 746 749 + BstKTI GATC 748 746 . . 746 749 + MboI GATC 745 749 . . 748 751 + TaqI TCGA 748 750 . . 750 754 + MboII GAAGA 762 761 . . 750 755 - Ksp632I* CTCTTC 745 748 . . 765 775 + MwoI GCNNNNNNNGC 771 768 . . 767 772 + Eco57MI CTGRAG 788 786 . . 767 772 + Eco57I CTGAAG 788 786 . . 769 773 + MboII GAAGA 781 780 . . 769 774 - Ksp632I* CTCTTC 764 767 . . 769 775 - SapI GCTCTTC 764 767 . . 780 783 + TaiI ACGT 783 779 . . 780 783 + SetI ASST 783 779 . . 780 783 + MaeII ACGT 780 782 . . 785 788 + CviRI* TGCA 786 786 . . 789 793 + TfiI GAWTC 789 792 . . 789 793 + HinfI GANTC 789 792 . . 796 799 + FatI CATG 795 799 . . 796 799 + NlaIII CATG 799 795 . . 796 799 + CviAII CATG 796 798 . . 815 820 + Hpy178III* TCNNGA 816 818 . . 825 829 + DdeI CTNAG 825 828 . . 825 829 + BspCNI CTCAG 838 836 . . 825 829 + BseMII CTCAG 839 837 . . 828 831 + SetI ASST 831 827 . . 832 835 + MseI TTAA 832 834 . . 837 841 + HinfI GANTC 837 840 . . 837 841 - PleI GAGTC 831 832 . . 841 844 + MnlI CCTC 851 850 . . 837 841 - MlyI GAGTC 831 831 . . 843 848 + Hpy178III* TCNNGA 844 846 . . 839 844 - BseRI GAGGAG 830 828 . . 842 847 - BsiI* CACGAG 842 846 . . 856 860 - TspDTI ATGAA 846 844 . . 860 864 - HphI GGTGA 852 851 . . 866 871 + EcoRV GATATC 868 868 . . 876 880 + BsmAI GTCTC 881 885 . . 882 885 + TspEI AATT 881 885 . . 886 890 + Tsp45I GTSAC 885 890 . . 886 890 + MaeIII GTNAC 885 890 . . 888 894 + TspRI CASTGNN 894 885 . . 890 894 + DdeI CTNAG 890 893 . . 893 899 + PpuMI RGGWCCY 894 897 . . 893 899 + DraII RGGNCCY 894 897 . . 890 894 - BspCNI CTCAG 882 880 . . 890 894 - BseMII CTCAG 881 879 . . 894 899 + NlaIV GGNNCC 896 896 . . 894 898 + BmgT120I GGNCC 895 896 . . 894 898 + AvaII GGWCC 894 897 . . 894 898 + AsuI* GGNCC 894 897 . . 892 895 - MnlI CCTC 885 884 . . 907 910 - MnlI CCTC 900 899 . . 921 925 + BssKI CCNGG 920 925 . . 921 925 + BseBI CCWGG 922 923 . . 921 925 + ScrFI CCNGG 922 923 . . 921 925 + EcoRII CCWGG 920 925 . . 923 926 + SetI ASST 926 922 . . 925 930 + Hpy166II GTNNAC 927 927 . . 925 930 + AccI GTMKAC 926 928 . . 944 947 - MnlI CCTC 937 936 . . 947 950 - AciI CCGC 947 949 . . 944 954 - Hin4I GAYNNNNNVTC 967 962 935 930 954 959 + BtrI CACGTC 956 956 . . 955 958 + TaiI ACGT 958 954 . . 955 958 + SetI ASST 958 954 . . 955 958 + MaeII ACGT 955 957 . . 958 962 + Hpy188I TCNGA 960 959 . . 961 965 + TfiI GAWTC 961 964 . . 961 965 + HinfI GANTC 961 964 . . 964 969 + MmeI TCCRAC 989 987 . . 972 975 + SetI ASST 975 971 . . 983 986 + MaeI CTAG 983 985 . . 1003 1008 + Hpy178III* TCNNGA 1004 1006 . . 1007 1017 + Hin4I GAYNNNNNVTC 998 993 1030 1025 1013 1017 + HinfI GANTC 1013 1016 . . 1013 1024 + DrdI GACNNNNNNGTC 1019 1017 . . 1013 1017 - PleI GAGTC 1007 1008 . . 1013 1017 - MlyI GAGTC 1007 1007 . . 1048 1053 + SfeI* CTRYAG 1048 1052 . . 1055 1060 + BsmI GAATGC 1061 1059 . . 1058 1061 + CviRI* TGCA 1059 1059 . . 1077 1080 + CviJI RGCY 1078 1078 . . 1081 1085 + Tsp45I GTSAC 1080 1085 . . 1081 1085 + MaeIII GTNAC 1080 1085 . . 1083 1089 + TspRI CASTGNN 1089 1080 . . 1084 1088 + Tsp4CI* ACNGT 1086 1085 . . 1089 1092 + CviJI RGCY 1090 1090 . . 1091 1095 + DdeI CTNAG 1091 1094 . . 1088 1093 - TsoI TARCCA 1078 1076 . . 1090 1096 - Bpu10I CCTNAGC 1091 1094 . . 1101 1104 + SetI ASST 1104 1100 . . 1102 1106 + Hin4II* CCTTC 1112 1111 . . 1106 1110 + DdeI CTNAG 1106 1109 . . 1116 1120 + BinI* GGATC 1124 1125 . . 1113 1117 - Hin4II* CCTTC 1107 1106 . . 1117 1120 + DpnI GATC 1118 1118 . . 1117 1120 + BstKTI GATC 1119 1117 . . 1117 1120 + MboI GATC 1116 1120 . . 1126 1130 + TauI GCSGC 1129 1126 . . 1126 1130 + BlsI GCNGC 1128 1127 . . 1126 1130 + BisI GCNGC 1127 1128 . . 1128 1131 + CviJI RGCY 1129 1129 . . 1129 1133 + BlsI GCNGC 1131 1130 . . 1129 1133 + BisI GCNGC 1130 1131 . . 1129 1133 + TseI GCWGC 1129 1132 . . 1126 1129 - AciI CCGC 1126 1128 . . 1129 1133 - BbvI GCAGC 1116 1120 . . 1130 1135 - EcoP15I CAGCAG 1102 1104 . . 1136 1140 + MaeIII GTNAC 1135 1140 . . 1142 1145 + MaeI CTAG 1142 1144 . . 1143 1148 + TsoI TARCCA 1159 1157 . . 1144 1147 + CviJI RGCY 1145 1145 . . 1146 1150 + BssKI CCNGG 1145 1150 . . 1146 1150 + BseBI CCWGG 1147 1148 . . 1146 1150 + ScrFI CCNGG 1147 1148 . . 1146 1150 + EcoRII CCWGG 1145 1150 . . 1158 1163 + Cac8I GCNNGC 1160 1160 . . 1168 1172 + Tsp4CI* ACNGT 1170 1169 . . 1185 1190 + Ksp632I* CTCTTC 1191 1194 . . 1186 1190 - MboII GAAGA 1178 1177 . . 1206 1210 + TfiI GAWTC 1206 1209 . . 1206 1210 + HinfI GANTC 1206 1209 . . 1214 1218 + MboII GAAGA 1226 1225 . . 1214 1219 + BbvII* GAAGAC 1221 1225 . . 1222 1227 + PstI CTGCAG 1226 1222 . . 1222 1227 + SfeI* CTRYAG 1222 1226 . . 1223 1226 + CviRI* TGCA 1224 1224 . . 1226 1231 + XhoII RGATCY 1226 1230 . . 1227 1230 + DpnI GATC 1228 1228 . . 1227 1230 + BstKTI GATC 1229 1227 . . 1227 1230 + MboI GATC 1226 1230 . . 1227 1231 - BinI* GGATC 1221 1222 . . 1241 1244 + CviJI RGCY 1242 1242 . . 1243 1247 + Hin4II* CCTTC 1253 1252 . . 1246 1250 + Hpy188I TCNGA 1248 1247 . . 1258 1261 + Hin6I GCGC 1258 1260 . . 1258 1261 + GlaI GCGC 1259 1259 . . 1258 1261 + HhaI GCGC 1260 1258 . . 1255 1259 - SfaNI GCATC 1245 1249 . . 1267 1272 + ZraI GACGTC 1269 1269 . . 1267 1272 + AcyI GRCGYC 1268 1270 . . 1267 1272 + AatII GACGTC 1271 1267 . . 1268 1271 + TaiI ACGT 1271 1267 . . 1268 1271 + SetI ASST 1271 1267 . . 1268 1271 + MaeII ACGT 1268 1270 . . 1270 1274 + Tsp45I GTSAC 1269 1274 . . 1270 1274 + MaeIII GTNAC 1269 1274 . . 1272 1278 + TspRI CASTGNN 1278 1269 . . 1276 1280 + HinfI GANTC 1276 1279 . . 1276 1280 - PleI GAGTC 1270 1271 . . 1276 1280 - MlyI GAGTC 1270 1270 . . 1281 1285 + Hpy188I TCNGA 1283 1282 . . 1286 1300 + XcmI CCANNNNNNNNNTGG 1293 1292 . . 1289 1292 + TaiI ACGT 1292 1288 . . 1289 1292 + SetI ASST 1292 1288 . . 1289 1292 + MaeII ACGT 1289 1291 . . 1291 1296 + Hpy166II GTNNAC 1293 1293 . . 1294 1304 + Hin4I GAYNNNNNVTC 1285 1280 1317 1312 1299 1302 + CviJI RGCY 1300 1300 . . 1299 1302 + HaeIII GGCC 1300 1300 . . 1301 1304 + MnlI CCTC 1311 1310 . . 1301 1311 + BsiYI* CCNNNNNNNGG 1307 1304 . . 1302 1307 + Ksp632I* CTCTTC 1308 1311 . . 1303 1307 - MboII GAAGA 1295 1294 . . 1309 1315 + PpuMI RGGWCCY 1310 1313 . . 1304 1309 - Eco57MI CTGRAG 1289 1287 . . 1309 1315 + DraII RGGNCCY 1310 1313 . . 1309 1314 + NlaIV GGNNCC 1311 1311 . . 1304 1309 - Eco57I CTGAAG 1289 1287 . . 1310 1314 + BmgT120I GGNCC 1311 1312 . . 1310 1314 + AvaII GGWCC 1310 1313 . . 1310 1314 + AsuI* GGNCC 1310 1313 . . 1313 1316 + MnlI CCTC 1323 1322 . . 1316 1319 + MnlI CCTC 1326 1325 . . 1314 1319 - BseRI GAGGAG 1305 1303 . . 1323 1328 + SduI GDGCHC 1327 1323 . . 1323 1328 + HgiJII* GRGCYC 1327 1323 . . 1324 1327 + CviJI RGCY 1325 1325 . . 1325 1331 + SapI GCTCTTC 1332 1335 . . 1326 1331 + Ksp632I* CTCTTC 1332 1335 . . 1327 1331 - MboII GAAGA 1319 1318 . . 1333 1337 + TauI GCSGC 1336 1333 . . 1333 1337 + BlsI GCNGC 1335 1334 . . 1333 1337 + BisI GCNGC 1334 1335 . . 1334 1339 + NspBII* CMGCKG 1336 1336 . . 1334 1337 + AciI CCGC 1334 1336 . . 1336 1346 + MwoI GCNNNNNNNGC 1342 1339 . . 1336 1340 + BlsI GCNGC 1338 1337 . . 1336 1340 + BisI GCNGC 1337 1338 . . 1336 1340 + TseI GCWGC 1336 1339 . . 1336 1340 - BbvI GCAGC 1323 1327 . . 1337 1342 - EcoP15I CAGCAG 1309 1311 . . 1349 1353 + DdeI CTNAG 1349 1352 . . 1358 1362 + MboII GAAGA 1370 1369 . . 1359 1369 + FalI AAGNNNNNCTT 1350 1345 1382 1377 1361 1365 + MboII GAAGA 1373 1372 . . 1361 1370 + XmnI GAANNNNTTC 1365 1365 . . 1363 1368 + XhoII RGATCY 1363 1367 . . 1363 1368 + BglII AGATCT 1363 1367 . . 1364 1367 + DpnI GATC 1365 1365 . . 1364 1367 + BstKTI GATC 1366 1364 . . 1364 1367 + MboI GATC 1363 1367 . . 1359 1369 - FalI AAGNNNNNCTT 1382 1377 1350 1345 1366 1370 - MboII GAAGA 1358 1357 . . 1368 1372 - TspDTI ATGAA 1358 1356 . . 1373 1378 + MluI ACGCGT 1373 1377 . . 1373 1378 + AflIII ACRYGT 1373 1377 . . 1374 1377 + FnuDII* CGCG 1375 1375 . . 1385 1389 + Tsp4CI* ACNGT 1387 1386 . . 1393 1398 + NlaIV GGNNCC 1395 1395 . . 1393 1398 + HgiCI* GGYRCC 1393 1397 . . 1401 1406 + ApoI RAATTY 1401 1405 . . 1402 1405 + TspEI AATT 1401 1405 . . 1426 1431 + ApoI RAATTY 1426 1430 . . 1427 1430 + TspEI AATT 1426 1430 . . 1429 1433 - TspDTI ATGAA 1419 1417 . . 1434 1439 + Hpy178III* TCNNGA 1435 1437 . . 1435 1440 + SmlI CTYRAG 1435 1439 . . 1435 1440 + Bce83I* CTTGAG 1456 1454 . . 1441 1444 + MseI TTAA 1441 1443 . . 1443 1446 + TspEI AATT 1442 1446 . . 1450 1454 + Tsp4CI* ACNGT 1452 1451 . . 1457 1461 + TspDTI ATGAA 1472 1470 . . 1459 1463 + MboII GAAGA 1471 1470 . . 1459 1464 - Ksp632I* CTCTTC 1454 1457 . . 1479 1482 + SetI ASST 1482 1478 . . 1479 1482 + CviJI RGCY 1480 1480 . . 1479 1482 + AluI AGCT 1480 1480 . . 1485 1490 + SmlI CTYRAG 1485 1489 . . 1485 1490 + Bce83I* CTTGAG 1506 1504 . . 1488 1491 - MnlI CCTC 1481 1480 . . 1494 1499 + BalI TGGCCA 1496 1496 . . 1494 1499 + CfrI YGGCCR 1494 1498 . . 1495 1498 + CviJI RGCY 1496 1496 . . 1495 1498 + HaeIII GGCC 1496 1496 . . 1496 1501 + Cac8I GCNNGC 1498 1498 . . 1512 1518 + TspRI CASTGNN 1518 1509 . . 1512 1521 + MslI CAYNNNNRTG 1516 1516 . . 1515 1518 + CviRI* TGCA 1516 1516 . . 1516 1521 + BsrDI GCAATG 1523 1521 . . 1512 1517 - BtsI GCAGTG 1511 1509 . . 1526 1532 + DraII RGGNCCY 1527 1530 . . 1527 1530 + CviJI RGCY 1528 1528 . . 1527 1530 + HaeIII GGCC 1528 1528 . . 1527 1531 + BmgT120I GGNCC 1528 1529 . . 1527 1531 + AsuI* GGNCC 1527 1530 . . 1525 1528 - MnlI CCTC 1518 1517 . . 1530 1534 + Hin4II* CCTTC 1540 1539 . . 1538 1541 + TaqI TCGA 1538 1540 . . 1539 1543 + Hpy99I CGWCG 1543 1538 . . 1544 1548 + Tsp4CI* ACNGT 1546 1545 . . 1550 1555 + NdeI CATATG 1551 1553 . . 1548 1552 - TspDTI ATGAA 1538 1536 . . 1556 1559 + TaiI ACGT 1559 1555 . . 1556 1559 + SetI ASST 1559 1555 . . 1556 1559 + MaeII ACGT 1556 1558 . . 1558 1563 + Hpy166II GTNNAC 1560 1560 . . 1569 1578 + MslI CAYNNNNRTG 1573 1573 . . 1567 1571 - TspDTI ATGAA 1557 1555 . . 1573 1576 + TaqI TCGA 1573 1575 . . 1575 1579 - SfaNI GCATC 1565 1569 . . 1593 1596 + SetI ASST 1596 1592 . . 1598 1603 + Hpy178III* TCNNGA 1599 1601 . . 1610 1613 + MaeI CTAG 1610 1612 . . 1613 1618 + ApoI RAATTY 1613 1617 . . 1614 1617 + TspEI AATT 1613 1617 . . 1619 1622 + MseI TTAA 1619 1621 . . 1621 1624 + TspEI AATT 1620 1624 . . 1635 1639 + BlsI GCNGC 1637 1636 . . 1635 1639 + BisI GCNGC 1636 1637 . . 1635 1639 + BbvI GCAGC 1647 1651 . . 1635 1639 + TseI GCWGC 1635 1638 . . 1643 1646 + TspEI AATT 1642 1646 . . 1655 1658 + CviRI* TGCA 1656 1656 . . 1664 1668 - MboII GAAGA 1656 1655 . . 1670 1674 + TspDTI ATGAA 1685 1683 . . 1673 1676 + TspEI AATT 1672 1676 . . 1685 1688 + CviRI* TGCA 1686 1686 . . 1738 1743 + MfeI CAATTG 1738 1742 . . 1739 1742 + TspEI AATT 1738 1742 . . 1743 1747 + MboII GAAGA 1755 1754 . . 1751 1754 + TaqI TCGA 1751 1753 . . 1753 1756 + DpnI GATC 1754 1754 . . 1753 1756 + BstKTI GATC 1755 1753 . . 1753 1756 + MboI GATC 1752 1756 . . 1766 1771 + BclI TGATCA 1766 1770 . . 1767 1770 + DpnI GATC 1768 1768 . . 1767 1770 + BstKTI GATC 1769 1767 . . 1767 1770 + MboI GATC 1766 1770 . . 1774 1777 + MseI TTAA 1774 1776 . . 1779 1782 + DpnI GATC 1780 1780 . . 1779 1782 + BstKTI GATC 1781 1779 . . 1779 1782 + MboI GATC 1778 1782 . . 1786 1790 + BsmAI GTCTC 1791 1795 . . 1810 1813 + TspEI AATT 1809 1813 . . 1817 1820 + TaqI TCGA 1817 1819 . . 1821 1824 + TspEI AATT 1820 1824 . . 1837 1842 + SfeI* CTRYAG 1837 1841 . . 1839 1843 + Tsp4CI* ACNGT 1841 1840 . . 1843 1846 + MseI TTAA 1843 1845 . . 1847 1851 + Hpy188I TCNGA 1849 1848 . . 1858 1863 + AclI AACGTT 1859 1861 . . 1859 1862 + TaiI ACGT 1862 1858 . . 1859 1862 + SetI ASST 1862 1858 . . 1859 1862 + MaeII ACGT 1859 1861 . . 1868 1873 + Hpy178III* TCNNGA 1869 1871 . . 1876 1879 + SetI ASST 1879 1875 . . 1877 1880 + MnlI CCTC 1887 1886 . . 1884 1887 + TspEI AATT 1883 1887 . . 1893 1898 + ApoI RAATTY 1893 1897 . . 1894 1897 + TspEI AATT 1893 1897 . . 1898 1903 + BclI TGATCA 1898 1902 . . 1899 1902 + DpnI GATC 1900 1900 . . 1899 1902 + BstKTI GATC 1901 1899 . . 1899 1902 + MboI GATC 1898 1902 . . 1901 1905 - HphI GGTGA 1893 1892 . . 1921 1925 + TauI GCSGC 1924 1921 . . 1921 1925 + BlsI GCNGC 1923 1922 . . 1921 1925 + BisI GCNGC 1922 1923 . . 1916 1921 - BsiI* CACGAG 1916 1920 . . 1922 1925 + AciI CCGC 1922 1924 . . 1926 1931 + VspI ATTAAT 1927 1929 . . 1927 1930 + MseI TTAA 1927 1929 . . 1939 1943 + TfiI GAWTC 1939 1942 . . 1939 1943 + HinfI GANTC 1939 1942 . . 1941 1945 - TspDTI ATGAA 1931 1929 . . 1945 1950 + Hpy178III* TCNNGA 1946 1948 . . 1948 1953 + XhoII RGATCY 1948 1952 . . 1948 1953 + BglII AGATCT 1948 1952 . . 1949 1952 + DpnI GATC 1950 1950 . . 1949 1952 + BstKTI GATC 1951 1949 . . 1949 1952 + MboI GATC 1948 1952 . . 1954 1959 + SspI AATATT 1956 1956 . . 1964 1967 + MnlI CCTC 1974 1973 . . 1968 1973 + Hpy178III* TCNNGA 1969 1971 . . 1982 1985 + FatI CATG 1981 1985 . . 1982 1985 + NlaIII CATG 1985 1981 . . 1982 1985 + CviAII CATG 1982 1984 . . 2003 2006 + TspEI AATT 2002 2006 . . 2013 2017 - TspDTI ATGAA 2003 2001 . . 2028 2032 + TfiI GAWTC 2028 2031 . . 2028 2032 + HinfI GANTC 2028 2031 . . 2032 2037 + PvuII CAGCTG 2034 2034 . . 2032 2037 + NspBII* CMGCKG 2034 2034 . . 2033 2036 + SetI ASST 2036 2032 . . 2033 2036 + CviJI RGCY 2034 2034 . . 2033 2036 + AluI AGCT 2034 2034 . . 2037 2047 + Hin4I GAYNNNNNVTC 2028 2023 2060 2055 2043 2053 + BsiYI* CCNNNNNNNGG 2049 2046 . . 2044 2047 + MnlI CCTC 2054 2053 . . 2047 2052 + StyI CCWWGG 2047 2051 . . 2047 2052 + SecI* CCNNGG 2047 2051 . . 2070 2073 + FatI CATG 2069 2073 . . 2070 2073 + NlaIII CATG 2073 2069 . . 2070 2073 + CviAII CATG 2070 2072 . . 2075 2080 + Hpy166II GTNNAC 2077 2077 . . 2097 2101 + FokI GGATG 2110 2114 . . 2097 2101 + BseGI GGATG 2103 2101 . . 2099 2103 + TspDTI ATGAA 2114 2112 . . 2122 2125 + MaeI CTAG 2122 2124 . . 2124 2127 + SetI ASST 2127 2123 . . 2124 2127 + CviJI RGCY 2125 2125 . . 2124 2127 + AluI AGCT 2125 2125 . . 2125 2135 + MwoI GCNNNNNNNGC 2131 2128 . . 2126 2129 + MaeI CTAG 2126 2128 . . 2135 2143 + DraIII CACNNNGTG 2140 2137 . . 2137 2141 + DdeI CTNAG 2137 2140 . . 2147 2150 + SetI ASST 2150 2146 . . 2147 2150 + CviJI RGCY 2148 2148 . . 2147 2150 + AluI AGCT 2148 2148 . . 2162 2166 + TfiI GAWTC 2162 2165 . . 2162 2166 + HinfI GANTC 2162 2165 . . 2174 2178 + TspDTI ATGAA 2189 2187 . . 2179 2182 + Csp6I GTAC 2179 2181 . . 2179 2182 + RsaI GTAC 2180 2180 . . 2180 2185 + BsaAI YACGTR 2182 2182 . . 2181 2184 + TaiI ACGT 2184 2180 . . 2181 2184 + SetI ASST 2184 2180 . . 2181 2184 + MaeII ACGT 2181 2183 . . 2186 2190 + TspDTI ATGAA 2201 2199 . . 2204 2207 + MseI TTAA 2204 2206 . . 2211 2216 + EciI GGCGGA 2227 2225 . . 2209 2212 - MnlI CCTC 2202 2201 . . 2212 2215 - AciI CCGC 2212 2214 . . 2218 2221 + SetI ASST 2221 2217 . . 2215 2219 - BsmAI GTCTC 2209 2213 . . 2215 2220 - Eco31I GGTCTC 2209 2213 . . 2240 2243 + TspEI AATT 2239 2243 . . 2253 2256 + SetI ASST 2256 2252 . . 2254 2257 + MnlI CCTC 2264 2263 . . 2255 2259 + DdeI CTNAG 2255 2258 . . 2255 2259 + BspCNI CTCAG 2268 2266 . . 2255 2259 + BseMII CTCAG 2269 2267 . . 2256 2260 + Hpy188I TCNGA 2258 2257 . . 2260 2263 + SetI ASST 2263 2259 . . 2261 2266 + Eco31I GGTCTC 2267 2271 . . 2259 2262 - MnlI CCTC 2252 2251 . . 2262 2266 + BsmAI GTCTC 2267 2271 . . 2264 2269 + SmlI CTYRAG 2264 2268 . . 2265 2270 + Hpy178III* TCNNGA 2266 2268 . . 2264 2269 - Bce83I* CTTGAG 2249 2247 . . 2270 2273 + TspEI AATT 2269 2273 . . 2284 2287 + MseI TTAA 2284 2286 . . 2281 2291 - Hin4I GAYNNNNNVTC 2304 2299 2272 2267 2298 2303 + PsiI TTATAA 2300 2300 . . 2328 2337 + XmnI GAANNNNTTC 2332 2332 . . 2328 2333 + ApoI RAATTY 2328 2332 . . 2329 2332 + TspEI AATT 2328 2332 . . 2341 2346 + AclI AACGTT 2342 2344 . . 2342 2345 + TaiI ACGT 2345 2341 . . 2342 2345 + SetI ASST 2345 2341 . . 2342 2345 + MaeII ACGT 2342 2344 . . 2361 2366 + Hpy178III* TCNNGA 2362 2364 . . 2361 2366 + NruI TCGCGA 2363 2363 . . 2362 2365 + FnuDII* CGCG 2363 2363 . . 2363 2368 + BtgZI GCGATG 2378 2382 . . 2368 2372 + HphI GGTGA 2380 2379 . . 2365 2369 - BccI CCATC 2359 2360 . . 2377 2381 + Hpy188I TCNGA 2379 2378 . . #--------------------------------------- # # # AatII: # [GACGTC] # 1120 1271 # # Acc65I: # [GGTACC] # 145 2246 # # AccI: # [GTMKAC] # 331 595 1465 # # AciI: # [CCGC] # 9 10 26 75 126 131 # 143 179 179 208 290 427 # 588 # # AclI: # [AACGTT] # 49 483 1859 # # AcyI: # [GRCGYC] # 143 212 913 1123 # # AflIII: # [ACRYGT] # 1018 1373 # # AluI: # [AGCT] # 23 91 243 244 414 554 # 822 # # AlwNI: # [CAGNNNCTG] # 247 456 1688 # # ApoI: # [RAATTY] # 25 63 187 280 435 477 # 924 # # AsuI*: # [GGNCC] # 7 23 43 48 66 69 # 110 112 194 217 222 416 # 864 # # AvaII: # [GGWCC] # 23 43 48 69 222 229 # 260 416 1081 # # AvrII: # [CCTAGG] # 268 2123 # # BalI: # [TGGCCA] # 895 1496 # # BbvCI: # [CCTCAGC] # 524 1867 # # BbvI: # [GCAGC] # 3 3 6 15 45 56 # 66 66 107 188 207 324 # 561 744 # # BbvII*: # [GAAGAC] # 1170 1221 # # BccI: # [CCATC] # 32 34 237 408 1680 # # Bce83I*: # [CTTGAG] # 50 112 142 743 1344 # # BceAI: # [ACGGC] # 60 239 2092 # # BclI: # [TGATCA] # 132 493 1766 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 118 2239 # # BglII: # [AGATCT] # 443 585 1363 # # BinI*: # [GGATC] # 97 1124 1170 # # BisI: # [GCNGC] # 3 3 3 3 3 3 # 3 15 22 23 53 66 # 66 107 177 204 286 299 # 469 583 # # BlsI: # [GCNGC] # 3 3 3 3 3 3 # 3 15 22 23 53 66 # 66 107 178 204 286 299 # 468 583 # # BmgT120I: # [GGNCC] # 7 23 44 48 66 69 # 110 112 194 217 222 416 # 863 # # Bpu10I: # [CCTNAGC] # 524 567 1300 # # BsaAI: # [YACGTR] # 209 2182 # # BsaXI: # [ACNNNNNCTCC] # 30 30 30 98 157 363 # 1683 # # BseBI: # [CCWGG] # 36 99 224 225 563 1244 # # BseGI: # [GGATG] # 45 288 450 1608 # # BseMII: # [CTCAG] # 42 122 301 538 1388 # # BseRI: # [GAGGAG] # 109 475 721 1086 # # BsgI: # [GTGCAG] # 320 2071 # # BsiI*: # [CACGAG] # 475 842 1074 # # BsiYI*: # [CCNNNNNNNGG] # 11 12 45 64 99 342 # 478 598 742 # # BslFI: # [GGGAC] # 551 1840 # # BsmAI: # [GTCTC] # 58 124 370 418 511 910 # # BsmI: # [GAATGC] # 1061 1330 # # Bsp1407I: # [TGTACA] # 639 1752 # # BspCNI: # [CTCAG] # 44 123 301 537 1386 # # BsrDI: # [GCAATG] # 356 868 1167 # # BsrI: # [ACTGG] # 649 1742 # # BssKI: # [CCNGG] # 36 99 224 225 561 1246 # # BstAPI: # [GCANNNNNTGC] # 247 2144 # # BstKTI: # [GATC] # 12 14 50 110 120 137 # 232 371 389 440 516 # # BtgZI: # [GCGATG] # 13 2378 # # BtrI: # [CACGTC] # 956 1435 # # BtsI: # [GCAGTG] # 880 1511 # # Cac8I: # [GCNNGC] # 82 338 466 612 893 # # CfrI: # [YGGCCR] # 897 1494 # # Csp6I: # [GTAC] # 146 212 225 269 1539 # # CviAII: # [CATG] # 88 307 321 489 1186 # # CviJI: # [RGCY] # 6 11 12 16 16 18 # 21 22 23 25 32 39 # 43 46 58 67 74 91 # 97 112 129 152 155 243 # 377 506 # # CviRI*: # [TGCA] # 9 30 30 54 81 87 # 95 140 165 189 241 273 # 292 705 # # DdeI: # [CTNAG] # 15 65 118 136 201 243 # 301 524 788 # # DinI: # [GGCGCC] # 356 2035 # # DpnI: # [GATC] # 12 14 50 110 120 137 # 231 371 389 441 516 # # DraII: # [RGGNCCY] # 66 69 194 217 270 295 # 416 864 # # DraIII: # [CACNNNGTG] # 251 2140 # # DrdI: # [GACNNNNNNGTC] # 452 567 1372 # # DsaI*: # [CCRYGG] # 221 267 1903 # # EciI: # [GGCGGA] # 164 509 1718 # # Eco31I: # [GGTCTC] # 58 124 511 1698 # # Eco57I: # [CTGAAG] # 328 460 501 1102 # # Eco57MI: # [CTGRAG] # 1 108 328 351 501 1102 # # EcoNI: # [CCTNNNNNAGG] # 695 1696 # # EcoP15I: # [CAGCAG] # 3 9 104 204 207 236 # 546 1082 # # EcoRII: # [CCWGG] # 36 99 224 225 561 1246 # # EcoRV: # [GATATC] # 868 1523 # # FalI: # [AAGNNNNNCTT] # 0 0 32 1009 1350 # # FatI: # [CATG] # 88 307 322 488 1186 # # FauI: # [CCCGC] # 29 2362 # # FnuDII*: # [CGCG] # 28 36 988 1339 # # FokI: # [GGATG] # 52 281 430 1628 # # GlaI: # [GCGC] # 35 133 225 321 545 1132 # # GsuI: # [CTGGAG] # 329 351 1711 # # HaeII: # [RGCGCY] # 358 2033 # # HaeIII: # [GGCC] # 32 140 196 266 295 599 # 863 # # HgaI: # [GACGC] # 201 210 338 1642 # # HgiAI*: # [GWGCWC] # 86 2305 # # HgiCI*: # [GGYRCC] # 72 137 145 998 1039 # # HgiJII*: # [GRGCYC] # 1064 1327 # # HhaI: # [GCGC] # 36 133 225 321 545 1131 # # Hin4I: # [GAYNNNNNVTC] # 31 32 32 32 32 32 # 87 212 255 711 935 # # Hin4II*: # [CCTTC] # 5 15 27 121 141 232 # 287 306 406 851 # # Hin6I: # [GCGC] # 34 133 225 321 545 1133 # # HindII: # [GTYRAC] # 559 1832 # # HindIII: # [AAGCTT] # 656 1735 # # HinfI: # [GANTC] # 48 52 70 89 124 134 # 171 193 229 299 319 663 # # HpaII: # [CCGG] # 382 2009 # # HphI: # [GGTGA] # 11 139 193 214 306 487 # 1041 # # Hpy166II: # [GTNNAC] # 180 188 227 267 314 332 # 366 517 # # Hpy178III*: # [TCNNGA] # 23 28 29 49 77 96 # 160 164 252 270 297 431 # 515 # # Hpy188I: # [TCNGA] # 12 35 121 288 409 566 # 960 # # Hpy99I: # [CGWCG] # 99 118 338 848 988 # # KasI: # [GGCGCC] # 354 2037 # # KpnI: # [GGTACC] # 149 2242 # # Ksp632I*: # [CTCTTC] # 19 24 53 117 122 427 # 692 937 # # MaeI: # [CTAG] # 4 32 42 159 227 265 # 468 512 682 # # MaeII: # [ACGT] # 21 49 161 175 267 303 # 313 322 327 453 # # MaeIII: # [GTNAC] # 55 64 134 195 821 1122 # # MboI: # [GATC] # 12 14 50 110 120 137 # 229 371 389 443 516 # # MboII: # [GAAGA] # 3 12 19 24 39 48 # 69 70 98 99 185 397 # 636 692 # # MfeI: # [CAATTG] # 197 653 1541 # # MluI: # [ACGCGT] # 1018 1373 # # MlyI: # [GAGTC] # 176 263 831 1121 # # MmeI: # [TCCRAC] # 282 707 1402 # # MnlI: # [CCTC] # 3 6 6 12 12 14 # 15 31 34 37 37 45 # 46 50 56 80 87 92 # 114 127 129 132 148 155 # 180 369 374 # # MseI: # [TTAA] # 69 80 84 107 155 178 # 227 277 605 609 # # MslI: # [CAYNNNNRTG] # 57 676 818 840 # # MwoI: # [GCNNNNNNNGC] # 3 3 59 182 243 260 # 281 571 789 # # NarI: # [GGCGCC] # 355 2036 # # NcoI: # [CCATGG] # 488 1903 # # NdeI: # [CATATG] # 234 840 1317 # # NlaIII: # [CATG] # 88 307 318 492 1186 # # NlaIV: # [GGNNCC] # 6 21 24 49 68 72 # 84 111 137 147 261 415 # 996 # # NruI: # [TCGCGA] # 28 2363 # # NspBII*: # [CMGCKG] # 9 179 357 698 1148 # # PflMI: # [CCANNNNNTGG] # 109 489 1793 # # PleI: # [GAGTC] # 176 263 831 1121 # # PpuMI: # [RGGWCCY] # 69 260 270 295 416 1081 # # PsiI: # [TTATAA] # 91 2300 # # PstI: # [CTGCAG] # 252 974 1165 # # PvuII: # [CAGCTG] # 357 2034 # # RsaI: # [GTAC] # 147 211 225 269 1539 # # SapI: # [GCTCTTC] # 568 764 1059 # # ScrFI: # [CCNGG] # 36 99 224 225 563 1244 # # SduI: # [GDGCHC] # 86 1064 1241 # # SecI*: # [CCNNGG] # 47 220 221 344 1559 # # SetI: # [ASST] # 6 7 8 17 17 21 # 23 24 32 34 35 37 # 37 37 46 48 54 63 # 77 82 91 95 103 111 # 111 123 129 143 157 167 # 190 266 # # SexAI: # [ACCWGGT] # 561 1830 # # SfaNI: # [GCATC] # 320 419 826 826 # # SfeI*: # [CTRYAG] # 164 174 248 554 615 636 # # SmlI: # [CTYRAG] # 50 91 127 779 1344 # # SspI: # [AATATT] # 435 1956 # # StyI: # [CCWWGG] # 220 268 344 1559 # # TaiI: # [ACGT] # 21 46 161 175 267 303 # 313 322 327 456 # # TaqI: # [TCGA] # 35 66 178 321 427 574 # 790 # # TaqII: # [GACCGA] # 289 395 1707 # # TatI: # [WGTACW] # 269 370 1752 # # TauI: # [GCSGC] # 9 75 182 207 467 588 # 863 # # TfiI: # [GAWTC] # 89 134 171 172 229 245 # 299 319 733 # # TseI: # [GCWGC] # 3 3 6 15 45 56 # 66 66 107 176 207 299 # 586 756 # # TsoI: # [TARCCA] # 81 1078 1232 # # Tsp45I: # [GTSAC] # 189 195 885 1122 # # Tsp4CI*: # [ACNGT] # 65 84 94 217 295 470 # 550 616 # # TspDTI: # [ATGAA] # 12 19 53 61 66 72 # 75 111 128 141 190 215 # 246 490 512 # # TspEI: # [AATT] # 7 10 11 16 22 25 # 30 30 42 55 59 60 # 63 63 66 71 109 171 # 197 220 237 307 520 # # TspRI: # [CASTGNN] # 189 195 240 873 894 # # TstI: # [CACNNNNNNTCC] # 32 32 32 149 155 310 # 1681 # # VspI: # [ATTAAT] # 464 1927 # # XbaI: # [TCTAGA] # 300 2091 # # XcmI: # [CCANNNNNNNNNTGG] # 1098 1293 # # XhoII: # [RGATCY] # 137 443 585 1226 # # XmnI: # [GAANNNNTTC] # 59 967 1365 # # ZraI: # [GACGTC] # 1122 1269 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2391 # Reported_sequences: 1 # Reported_hitcount: 747 #---------------------------------------