Chromosome I History Help



 

This page lists all sequence and annotation changes that have been made to the Chromosome I systematic reference sequence since its intial release on 1996-07-31.


SEQUENCE CHANGES, including any resulting annotation changesJump to: Annotation changes

DateAffected FeaturesStart Coordinate
of Change
End Coordinate
of Change
Type of ChangeOld SequenceNew Sequence
2011-02-03
YAL067C, YAL067W-A
38363836DeletionC
39813982SubstitutionATTA
52445244SubstitutionGA
59275927Insertion G
64546454DeletionC
 Several nucleotide sequence changes were made in the intergenic region between ORFs YAL067W-A and SEO1/YAL067C.
New    3791    CTTGGTAGTAACCATAATATTACCCAGGTACGAAACGCTAAGAAC-TTGAAAGACTCATA  3849
               ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
Old    3791    CTTGGTAGTAACCATAATATTACCCAGGTACGAAACGCTAAGAACCTTGAAAGACTCATA  3850
New    3960    TTTCCATTTTGATAATGAGCTAGTGATCCGGAAAGCTACTTTATGATGTTTCAAGGCCTG  4019
               ||||||||||||||||||||  ||||||||||||||||||||||||||||||||||||||
Old    3961    TTTCCATTTTGATAATGAGCATGTGATCCGGAAAGCTACTTTATGATGTTTCAAGGCCTG  4020
New    5220    TAAATAGTTTTCAACTGCTGGTGATAAATCAATAATTTATGTTCTTAACCTAACATTTGA  5279
               ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
Old    5221    TAAATAGTTTTCAACTGCTGGTGGTAAATCAATAATTTATGTTCTTAACCTAACATTTGA  5280
New    5880    AACTGCATTCGGACTATGAAAGAAAAAATGGTAGTAGCAAAGGATAGGCATCGCCGTATT  5939
               ||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
Old    5881    AACTGCATTCGGACTATGAAAGAAAAAATGGTAGTAGCAAAGGATAG-CATCGCCGTATT  5939
New    6420    AAGTTTCTTAAATAACCCGGATTGGTTAGGTTCA-GCCATGCCTGGCGCGTACATTGAGG  6478
               |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
Old    6420    AAGTTTCTTAAATAACCCGGATTGGTTAGGTTCACGCCATGCCTGGCGCGTACATTGAGG  6479

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03
YAL064C-A, YAL064W
1645716457SubstitutionGA
1647016470DeletionT
1650016500DeletionG
 A single nucleotide substitution and two separate single nucleotide deletions were made in the intergenic region between ORFs YAL064C-A and YAL064W.
New    16449   TCTGTGGAAAATAAGAAATT-CAGCACCAGTAAAAGACGAGAAATATAGG-CACATAAAT  16506
               ||||||| |||||||||||| ||||||||||||||||||||||||||||| |||||||||
Old    16450   TCTGTGGGAAATAAGAAATTTCAGCACCAGTAAAAGACGAGAAATATAGGGCACATAAAT  16509

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03YAL064W2153121531Insertion A
 A single A nucleotide was inserted within ORF YAL064W, near its 5' end, moving the start codon out of frame with the rest of the protein. The C-terminus and majority of the reading frame remain the same, but the N-terminus has changed and the annotated protein is now 14 amino acids shorter.
New    21487   CTATCGATGTGTATACAAACGTACTTCAAATAAGCAATGCGAATATACTGCAACTTTTCG  21546
               |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
Old    21490   CTATCGATGTGTATACAAACGTACTTCAAATAAGCAATGCGA-TATACTGCAACTTTTCG  21548

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03YAL063C, YAL063C-A2325323253Insertion T
 A single nucleotide insertion was made in the intergenic region between ORFs YAL063C-A and FLO9/YAL063C.
New    23217   TTTGCTCATCATTGGATAATTTCTTTTTTTTTTTTTGATGCATCCAAACTTGGACCCCTT  23276
               ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Old    23219   TTTGCTCATCATTGGATAATTTCTTTTTTTTTTTT-GATGCATCCAAACTTGGACCCCTT  23277

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03
ARS104, YAL063C
2806928069DeletionT
2895328953Insertion T
 A single nucleotide deletion and a single nucleotide insertion were made in the intergenic region between ORF FLO9/YAL063C and ARS104.
New    28027   GTAAATAAGAAGGAAGAACGTTATGTTATTAATGGACTTTT-AGTGTCATCGAATTTTAT  28085
               ||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
Old    28028   GTAAATAAGAAGGAAGAACGTTATGTTATTAATGGACTTTTTAGTGTCATCGAATTTTAT  28087
New    28916   AGTAAACAAGAGATACCTAATTTCACAGCCACTTTTTGTTGCGGACACTGACGGGATGTG  28975
               |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
Old    28918   AGTAAACAAGAGATACCTAATTTCACAGCCACTTTT-GTTGCGGACACTGACGGGATGTG  28976

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

1998-09-02
YAL062W, YAL063C
2925429254DeletionA
2928929289DeletionA
2929029290DeletionA
2929129291DeletionT
2930129301SubstitutionTA
2930729307DeletionA
 The following changes were made to the systematic sequence of Chromosome I in the region encompassing features FLO9/YAL063C and GDH3/YAL062W: deletion of the A at 29254, deletion of AAT at 29289-29291, transversion of T to A at 29301, and deletion of the A at 29307.
Old: 29221 TGAAATCATACCTGCCAAGCTTGCTGCACTTGGAAAGGCAACTCCGTTTTTCAGAGATTA 29280
           ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
New: 29221 TGAAATCATACCTGCCAAGCTTGCTGCACTTGGAA-GGCAACTCCGTTTTTCAGAGATTA 29279
Old: 29281 GATCCTTTAATTTTTTTTGATGAAAAAGGCAGCCAAGTTACGTCATAGAGAAAACTCCCT 29340
           ||||||||   ||||||||| ||||| |||||||||||||||||||||||||||||||||
New: 29280 GATCCTTT---TTTTTTTGAAGAAAA-GGCAGCCAAGTTACGTCATAGAGAAAACTCCCT 29335
2011-02-03YAL060W3612036120SubstitutionCA
 A single nucleotide substitution within the coding region of BDH1/YAL060W resulted in an altered protein sequence. The start, stop, and reading frame remain the same, but protein residue 322 is now Aspartic Acid rather than Alanine.
New    36106   TATGTTGTCGAAGACTTCGAAGAAGTTGTTCGTGCCATCCACAACGGAGACATCGCCATG  36165
               ||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
Old    36107   TATGTTGTCGAAGCCTTCGAAGAAGTTGTTCGTGCCATCCACAACGGAGACATCGCCATG  36166

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03YAL059C-A, YAL059W3681436814SubstitutionAC
 A single nucleotide substitution within the coding regions of overlapping ORFs ECM1/YAL059W and YAL059C-A resulted in altered protein sequences for both ORFs. The start, stop, and reading frames remain the same for both proteins, but ECM1/YAL059W protein residue 102 is now Alanine rather than Aspartic Acid, and YAL059C-A protein residue 36 is now Alanine rather than Serine.
New    36776   AAGAAGTTAAATAAAAAGGCTCTGGAAGACAAACTGGCCAACTCTATTTCATCCATGGAC  36835
               ||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
Old    36777   AAGAAGTTAAATAAAAAGGCTCTGGAAGACAAACTGGACAACTCTATTTCATCCATGGAC  36836

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03
YAL056W
4023140231SubstitutionCG
4124041240SubstitutionAG
4166441664SubstitutionTG
4170041700SubstitutionCA
4170341703SubstitutionCA
 Nucleotide changes within the coding region of GPB2/YAL056W resulted in an altered protein sequence. The start, stop, and reading frame remain the same, but protein residue 661 is now Valine rather than Isoleucine, residue 802 is now Cysteine rather than Phenylalanine, and residues 814-815 are now ED rather than AA.
New    40196   ATTTTTTTGTAGACGGAATAAAGAACTTACCTCCGCCTTTACTACCTCAAGTGATTAATA  40255
               |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
Old    40197   ATTTTTTTGTAGACGGAATAAAGAACTTACCTCCCCCTTTACTACCTCAAGTGATTAATA  40256

New    41216   ACGATAATTTGGAAAATTATATAGTCAATCCAGGGAGAAAATCGTCATCTATTCCAATGA  41275
               ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
Old    41217   ACGATAATTTGGAAAATTATATAATCAATCCAGGGAGAAAATCGTCATCTATTCCAATGA  41276

New    41651   TAAGCACGCAGTGTTGGGAAGAACATAAAATTACTCTGTCCAAGAAGGAAGACGATGAGG  41710
               |||||||||||| ||||||||||||||||||||||||||||||||||| || ||||||||
Old    41652   TAAGCACGCAGTTTTGGGAAGAACATAAAATTACTCTGTCCAAGAAGGCAGCCGATGAGG  41711

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2004-01-24YAL056W4177841778InsertionG
 Kellis et al. 2003 predicted and confirmed the insertion of a single G nucleotide after the T at chromosomal coordinate 41778. As a consequence of this sequence change, YAL056W was extended at the 3' end, altering the C-terminus and increasing the size of the predicted protein increasing from 847 to 880 amino acids.
New: 41724 GCGAAAATGAAGATACAAATTCAAATATAGTAGTTGGTGTCGGTGGCACTTCTTTGCAAT 41783
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||| 
Old: 41724 GCGAAAATGAAGATACAAATTCAAATATAGTAGTTGGTGTCGGTGGCACTTCTTT-CAAT 41782 

Kellis M, et al. (2003) Sequencing and comparison of yeast species to identify genes and regulatory elements. Nature 423(6937):241-54
SGD Papers Entry  Pubmed Entry  DOI full text  Web Supplement  SGD Curated Comments & Errata

2011-02-03
YAL053W
4623146231SubstitutionCG
4683346833SubstitutionGA
4782147821SubstitutionTA
4782647826SubstitutionCT
 Nucleotide changes within the coding region of FLC2/YAL053W resulted in an altered protein sequence. The start, stop, and reading frame remain the same, but protein residue 111 is now Cysteine rather than Serine, residue 312 is now Asparagine rather than Aspartic Acid, and residues 641-643 are now NDS rather than IDP.
New    46196   TTTGATTTATGTTCCTTGGGCCAAGTATCGCTTTGCCCCCTAAGTGCTGGGCGTATTGAT  46255
               |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
Old    46197   TTTGATTTATGTTCCTTGGGCCAAGTATCGCTTTCCCCCCTAAGTGCTGGGCGTATTGAT  46256

New    46796   AGTGATTACAATTTTGACACCATTTTAGACGATTCGAATCTGTACACCACTTCTGAGAAG  46855
               |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
Old    46797   AGTGATTACAATTTTGACACCATTTTAGACGATTCGGATCTGTACACCACTTCTGAGAAG  46856

New    47806   TGGCAAAAACAAAAATGATTCTGAACTGTTTGAATTGAGAAAAGCTGTTATGGACACCAA  47865
               |||||||||||||| |||| ||||||||||||||||||||||||||||||||||||||||
Old    47807   TGGCAAAAACAAAATTGATCCTGAACTGTTTGAATTGAGAAAAGCTGTTATGGACACCAA  47866

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03
YAL051W
4877248772SubstitutionTA
4990449904SubstitutionCA
5032750327SubstitutionCA
 Nucleotide changes within the coding region of OAF1/YAL051W resulted in an altered protein sequence. The start, stop, and reading frame remain the same, but protein residue 70 is now Arginine rather than Tryptophan, residue 447 is now Glutamine rather than Proline, and residue 588 is now Lysine rather than Threonine.
New    48726   CATAATAGGAAAAGAAATAGAATATTGTTTGTCTGCCAGGCTTGTAGGAAGTCAAAAACA  48785
               ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
Old    48727   CATAATAGGAAAAGAAATAGAATATTGTTTGTCTGCCAGGCTTGTTGGAAGTCAAAAACA  48786

New    49856   AATAATTGATAAATACCCAATACCGAACGATTTTATTTTATTGAGTCAAAGATGTCTAGC  49915
               ||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
Old    49857   AATAATTGATAAATACCCAATACCGAACGATTTTATTTTATTGAGTCCAAGATGTCTAGC  49916

New    50286   AACCGAGTTAGGGGCGATCTAAGCGATATCAATAATCACAAACTTTTGAGAATTCATAAA  50345
               |||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
Old    50287   AACCGAGTTAGGGGCGATCTAAGCGATATCAATAATCACACACTTTTGAGAATTCATAAA  50346

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2004-07-20
YAL051W
5168851688DeletionG
5169451694DeletionG
 The work of Kellis et al. 2003 predicted the deletion of 2 separate G nucleotides in YAL051W at chromosomal coordinates 51688 and 51694, and these sequence errors were confirmed in S288C by SGD. As a consequence of these changes, YAL051W was shortened at the 3' end, altering the C-terminus and decreasing the size of the predicted protein from 1062 to 1047 amino acids.
NEW: 51666 TTTATTTGATTATGACTTTTTG-TTTGG-CAATGACTTTGCTTAAAAATTTTCTTTCCAA 51723
           |||||||||||||||||||||| ||||| |||||||||||||||||||||||||||||||
OLD: 51666 TTTATTTGATTATGACTTTTTGGTTTGGGCAATGACTTTGCTTAAAAATTTTCTTTCCAA 51725 

Kellis M, et al. (2003) Sequencing and comparison of yeast species to identify genes and regulatory elements. Nature 423(6937):241-54
SGD Papers Entry  Pubmed Entry  DOI full text  Web Supplement  SGD Curated Comments & Errata

2011-02-03
YAL047C
5574655746SubstitutionAG
5595455954SubstitutionAT
 Nucleotide changes within the coding region of SPC72/YAL047C resulted in an altered protein sequence. The start, stop, and reading frame remain the same, but protein residue 302 is now Asparagine rather than Isoleucine.
New    55726   TTTCCAAATTGTTTATCAAGACAGATTGCGATTCGATCTTTTCCTTCAGTTGGGAAATCA  55785
               ||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
Old    55727   TTTCCAAATTGTTTATCAAAACAGATTGCGATTCGATCTTTTCCTTCAGTTGGGAAATCA  55786

New    55916   AATGGAATTTATAAATTGATCATATTCTTTGTGCAAATTTTCTATGACAATCTCTAATTG  55975
               ||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
Old    55917   AATGGAATTTATAAATTGATCATATTCTTTGTGCAAAATTTCTATGACAATCTCTAATTG  55976

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2008-03-05
YAL044C
5819658196SubstitutionAC
5824758247SubstitutionAC
5842458424SubstitutionCT
 Three single base substitutions were made within ORF YAL044C/GCV3 to correct errors in the systematic reference sequence for Chromosome I: Two separate A -> C transversions at 58196 and 58247, and a C -> T transition at 58424. These errors were verified by sequencing in 3 different strains, all of ResGen BY4741 (S288C) background. SGD thanks Michael E. Rice for bringing these errors to our attention, and for verifying the correct sequence.
New:   58191 TTGGGCAATCTCAGTGCCCACTTCTGGCAACTCAACATAGGTAGCGTCCCCTAAGGCATC 58250
             ||||| |||||||||||||||||||||||||||||||||||||||||||||||||| ||| 
Old:   58191 TTGGGAAATCTCAGTGCCCACTTCTGGCAACTCAACATAGGTAGCGTCCCCTAAGGAATC 58250

New:   58251 AGTGGCGTATTTTGTAATTCCGACAAAGGCAGTCTTGTCCTGATGCACAGCTATCCACTC 58310
             |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
Old:   58251 AGTGGCGTATTTTGTAATTCCGACAAAGGCAGTCTTGTCCTGATGCACAGCTATCCACTC 58310

New:   58311 ATGTTGGGAAGTGTACCTCACGGCTTGAGGTCCTTGGGATGAGTACAAAAATGGTAGTTT 58370
             |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
Old:   58311 ATGTTGGGAAGTGTACCTCACGGCTTGAGGTCCTTGGGATGAGTACAAAAATGGTAGTTT 58370

New:   58371 ATTCTTGTTTAGGGCATTGCCGGAGCTGTTTCTCAAAAACAATTTGCTCACAGTGGGCAT 58430
             ||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||| 
Old:   58371 ATTCTTGTTTAGGGCATTGCCGGAGCTGTTTCTCAAAAACAATTTGCTCACAGCGGGCAT 58430
2011-02-03YAL041W, YAL042W6276762767SubstitutionAG
 A single nucleotide substitution was made in the intergenic region between ORFs ERV46/YAL042W and CDC24/YAL041W.
New    62756   TAGGATAGCAGAAGAGTACCATTGCTGTTATCATTTGTTGCCTAGCCCTATCAAGACCTG  62815
               |||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
Old    62757   TAGGATAGCAAAAGAGTACCATTGCTGTTATCATTTGTTGCCTAGCCCTATCAAGACCTG  62816

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03
ARS106, YAL038W
7079470794SubstitutionCG
7087470874SubstitutionAG
 Two separate single nucleotide substitutions were made in the intergenic region between ARS106 and ORF CDC19/YAL038W.
New    70746   GGTTTTCCGACTTTATTTGAGATGACTTGAGATGTGTGTCAATGCTAGTATTTTGGAGAT  70805
               ||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
Old    70747   GGTTTTCCGACTTTATTTGAGATGACTTGAGATGTGTGTCAATGCTACTATTTTGGAGAT  70806
New    70856   TTATTTTTTTTTTGTTAGAATTGATCCAAATGTAAATAAACAATCACAAGGAAAAAAAAA  70915
               ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
Old    70857   TTATTTTTTTTTTGTTAAAATTGATCCAAATGTAAATAAACAATCACAAGGAAAAAAAAA  70916

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03
YAL026C
9674096740SubstitutionGC
9683896838DeletionC
9684196841Insertion C
9702597026SubstitutionATTA
9767897679SubstitutionCCGG
9835098351SubstitutionCGGC
9956399565SubstitutionATCTCG
 Nucleotide changes within the coding region of DRS2/YAL026C resulted in an altered protein sequence. The start, stop, and reading frame remain the same, but protein residues 45-46 are now AN rather than GY, residue 450 is now Alanine rather than Arginine, residue 674 is now Proline rather than Glycine, residues 891-892 are now NT rather than KS, residues 953-954 are now GD rather than AS, and residue 987 is now Valine rather than Leucine.
New    96716   TTTTAAAAATTTAAATTGGCCAACAGCTATATCAGCTGAACGAGCCGCTTGCATACCTTC  96775
               ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
Old    96717   TTTTAAAAATTTAAATTGGCCAAGAGCTATATCAGCTGAACGAGCCGCTTGCATACCTTC  96776

New    96826   CGTTGGCACCA-TCGCCAATGGCTAGCAGTAGTGAAGACGACTTTCTTTTTACCATTTTA  96884
               ||||||||||| ||| ||||||||||||||||||||||||||||||||||||||||||||
Old    96827   CGTTGGCACCACTCG-CAATGGCTAGCAGTAGTGAAGACGACTTTCTTTTTACCATTTTA  96885

New    97005   TCAATGACGAGCGCTAAGGTATTCATATCATGTGTTGACAATTGATGCTCGTTTAGAGCG  97064
               |||||||||||||||||||  |||||||||||||||||||||||||||||||||||||||
Old    97006   TCAATGACGAGCGCTAAGGATTTCATATCATGTGTTGACAATTGATGCTCGTTTAGAGCG  97065

New    97665   GTTACAGAGTTTGGTTTACGGATGATAAACTTATACCCTAAATCTGCACCACCTTGAACG  97724
               ||||||||||||  ||||||||||||||||||||||||||||||||||||||||||||||
Old    97666   GTTACAGAGTTTCCTTTACGGATGATAAACTTATACCCTAAATCTGCACCACCTTGAACG  97725

New    98335   AAACTGTGAACAATGCAATAATCTGTCTGTTGATAATTTTCTCAACCGCGGTTCTTTTAA  98394
               ||||||||||||||  ||||||||||||||||||||||||||||||||||||||||||||
Old    98336   AAACTGTGAACAATCGAATAATCTGTCTGTTGATAATTTTCTCAACCGCGGTTCTTTTAA  98395

New    99535   GAAGTACATGACTTGGTGGTATATAATTCGCGTTCGCATGTGAGTTGGTGACCTTTGAAC  99594
               |||||||||||||||||||||||||||   ||||||||||||||||||||||||||||||
Old    99536   GAAGTACATGACTTGGTGGTATATAATATCCGTTCGCATGTGAGTTGGTGACCTTTGAAC  99595

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03HRA19984199841SubstitutionAT
 A single nucleotide substitution was made within ncRNA HRA1.
New    99825   TTCAACTTAGTGGCATTAAACAGATTTGGGTTTTCTGGCAAAAAAAGCCAATCACGTGAT  99884
               ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
Old    99826   TTCAACTTAGTGGCAATAAACAGATTTGGGTTTTCTGGCAAAAAAAGCCAATCACGTGAT  99885

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03YAL025C100399100399SubstitutionAC
 A single nucleotide substitution within the coding region of MAK16/YAL025C resulted in an altered protein sequence. The start, stop, and reading frame remain the same, but protein residue 250 is now Glutamic Acid rather than Glutamine.
New    100375  GCTTTCACTGTCGCTTTCGCTTTCACTGGCGCTGGAAGCTTCTCTGTCAGAGTCAGCTAA  100434
               ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
Old    100376  GCTTTCACTGTCGCTTTCGCTTTGACTGGCGCTGGAAGCTTCTCTGTCAGAGTCAGCTAA  100435

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2002-12-17
YAL023C
108137108137InsertionCC
108143108143InsertionG
 Due to the insertion of CC after the C at 108137, and the insertion of a G after the G at 108143 in the systematic sequence of Chromosome I, the coordinates of PMT2/YAL023C have been changed. Thanks to Verena Girrbach (verena.girrbach@biologie.uni-regensburg.de) for reporting this sequence error in the systematic sequence of PMT2/FUN25/YAL023C to SGD.
Old: 108121 AGTCTGGGTAAATTTCC--AGAAGG-AAGTCCCAAGAACCGTTGTAGCCTGCCAAATAAC 108177
            |||||||||||||||||  |||||| ||||||||||||||||||||||||||||||||||
New: 108121 AGTCTGGGTAAATTTCCCCAGAAGGGAAGTCCCAAGAACCGTTGTAGCCTGCCAAATAAC 108180
2011-02-03YAL021C, YAL022C110470110471SubstitutionCGGC
 A dinucleotide substitution was made in the intergenic region between ORFs FUN26/YAL022C and CCR4/YAL021C.
New    110455  GCGTTTGATGATAAGCTGTCGCCGTAACGTAAATCATTCATCCTTTCCTATGATTTTTTA  110514
               ||||||||||||||  ||||||||||||||||||||||||||||||||||||||||||||
Old    110456  GCGTTTGATGATAACGTGTCGCCGTAACGTAAATCATTCATCCTTTCCTATGATTTTTTA  110515

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03YAL020C113702113703SubstitutionCGGC
 The substitution of two nucleotides within the coding region of ATS1/YAL020C resulted in an altered protein sequence. The start, stop, and reading frame remain the same, but protein residue 305 is now Glycine rather than Alanine.
New    113685  GCAGTCCAGGCTGGGAGCCCTTTTGCGGGCCGCAGTTGCCATGCTCTCCCCAGCCCCAGC  113744
               ||||||||||||||||  ||||||||||||||||||||||||||||||||||||||||||
Old    113896  GCAGTCCAGGCTGGGACGCCTTTTGCGGGCCGCAGTTGCCATGCTCTCCCCAGCCCCAGC  113745

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03YAL017W120442120442SubstitutionCG
 A single nucleotide substitution within the coding region of PSK1/YAL017W resulted in an altered protein sequence. The start, stop, and reading frame remain the same, but protein residue 73 is now Glutamic Acid rather than Glutamine.
New    120415  CGAATAAAAAGGAAGGTGATGAGTTCGAGCAAAGTTTAAGAGATACATTTGCGAGCTTTC  120474
               |||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
Old    120416  CGAATAAAAAGGAAGGTGATGAGTTCCAGCAAAGTTTAAGAGATACATTTGCGAGCTTTC  120475

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2002-12-17YAL014C128441128441InsertionT
 Due to the insertion of a single T nucleotide after the T at coordinate 128441 in the systematic sequence of Chromosome I, the ORF SUN8/YAL014C was extended 450 nucleotides in the 3' direction, making the protein product 150 amino acids longer at the C-terminus. Thanks to Lena Burri, Trevor Lithgow, and Hugh Pelham for reporting this sequence error in the systematic sequence of SYN8/UIP2/YAL014C to SGD.

Old: 128398 TTCTAAATCAACCAGCAGCGAGTCGTTCTGAGAAACGATCTCAT-ATTAAGGTCCAGCGA 128456
            |||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
New: 128401 TTCTAAATCAACCAGCAGCGAGTCGTTCTGAGAAACGATCTCATTATTAAGGTCCAGCGA 128460

Lewis MJ and Pelham HR (2002) A new yeast endosomal SNARE related to mammalian syntaxin 8. Traffic 3(12):922-9
SGD Papers Entry  Pubmed Entry  DOI full text  

2003-12-17
YAL013W
130239130239InsertionC
130246130247SubstitutionCGGC
 Kellis et al. 2003 and Brachat et al. 2003 predicted and confirmed the insertion of a single C nucleotide after the C at position 130239 on Chromosome I. As a consequence of this sequence change, YAL013W was extended at the 3' end, altering the C-terminus and increasing the size of the predicted protein from 362 to 420 amino acids. They also found an additional seqeuncing error: the CG at 130246-130247 should be GC.

Old: 130201 ACTTGATAACAAGACGCTGAGCTGTATCACGGGCTACGC-AGCGCACGACAGCTGTGCTA 130259
            ||||||||||||||||||||||||||||||||||||||| ||||||  ||||||||||||
New: 130201 ACTTGATAACAAGACGCTGAGCTGTATCACGGGCTACGCCAGCGCAGCACAGCTGTGCTA 130260

Kellis M, et al. (2003) Sequencing and comparison of yeast species to identify genes and regulatory elements. Nature 423(6937):241-54
SGD Papers Entry  Pubmed Entry  DOI full text  Web Supplement  SGD Curated Comments & Errata
Brachat S, et al. (2003) Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii. Genome Biol 4(7):R45
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  

2011-02-03YAL013W130485130486DeletionGT
 Two nucleotides were deleted near the 3' end of ORF DEP1/YAL013W, altering its coding sequence. The start and most of the reading frame remain the same, but the C-terminus has changed and the annotated protein is now 15 amino acids shorter.
New    130445  AGACTCCGAAATCAACGACGACTTCCACCAGTGGGCCCA--GTGACCGCCACACTGGACC  130502
               |||||||||||||||||||||||||||||||||||||||  |||||||||||||||||||
Old    130446  AGACTCCGAAATCAACGACGACTTCCACCAGTGGGCCCAGTGTGACCGCCACACTGGACC  130505

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03YAL010C, YAL011W134125134125Insertion C
 A single nucleotide substitution was made in the intergenic region between ORFs SWC3/YAL011W and MDM10/YAL010C.
New    134093  ATTCTGCTTTAACGCCATTATGATTATACACATTGTATTACTTATTTTTTAACCTGTATA  134152
               |||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Old    134096  ATTCTGCTTTAACGCCATTATGATTATACA-ATTGTATTACTTATTTTTTAACCTGTATA  134154

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03YAL010C134852134854SubstitutionTTGATT
 A single nucleotide substitution within the coding region of MDM10/YAL010C resulted in an altered protein sequence. The start, stop, and reading frame remain the same, but protein residue 272 is now Asparagine rather than Glutamine.
New    134813  TGGCCGAATATGTGGAGGATATATGGCCGAATAATGGATTCCAAGATAATGTCAAAGTTA  134872
               ||||||||||||||||||||||||||||||||||||| | ||||||||||||||||||||
Old    134815  TGGCCGAATATGTGGAGGATATATGGCCGAATAATGGTTGCCAAGATAATGTCAAAGTTA  134874

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2007-04-05
YAL004W, YAL005C
140169140169SubstitutionAG
140182140182SubstitutionAG
140811140811SubstitutionAG
 Three single nucleotide substitutions were made within the coding sequence of SSA1 (at nucleotide positions 623, 1252, and 1265 relative to the SSA1 coding sequence), based on the sequence reported by Slater and Craig (1989) and corresponding to GenBank entry X12926. These changes result in phenylalanine to serine changes at amino acid residues 208 and 422 and a change from serine to proline at amino acid residue 418. Thanks to Andreas Bracher for bringing these corrections to our attention.

Note that the change at position 623 of SSA1 also affects the coding sequence of the Dubious ORF YAL004W.

638     ATACCGTCTTCAATGGACAACAAAGAGAC 610     new sequence, coordinates relative to SSA1 coding region
        ||||||||||||||| |||||||||||||       
140796  ATACCGTCTTCAATGAACAACAAAGAGAC 140824  old sequence, chromosomal coordinates


1279   TGGAAAAGATCTCGGACTTCTTTGTTGGAATGGTAGAGTTT 1239    new sequence, coordinates relative to SSA1 coding region
       |||||||||||||| |||||||||||| |||||||||||||
140155 TGGAAAAGATCTCGAACTTCTTTGTTGAAATGGTAGAGTTT 140195  old sequence, chromosomal coordinates

Slater MR and Craig EA (1989) The SSA1 and SSA2 genes of the yeast Saccharomyces cerevisiae. Nucleic Acids Res 17(2):805-6
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  

2004-01-22YAL002W143848143848InsertionC
 Kellis et al. 2003 and Cliften et al. 2003 predicted and confirmed the insertion of a single C nucleotide after the C at chromosomal coordinate 143848. As a consequence of this sequence change, YAL002W was extended at the 5' end, altering the N-terminus and increasing the size of the predicted protein from 1176 to 1274 amino acids.
New: 143826 ATCTAGTTCTTCATATACGGCACCTCCCCTGAACGAAGATGGTCCTAAAGGGGTAGCTTC 143885
            ||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||| 
Old: 143826 ATCTAGTTCTTCATATACGGCAC-TCCCCTGAACGAAGATGGTCCTAAAGGGGTAGCTTC 143884

Kellis M, et al. (2003) Sequencing and comparison of yeast species to identify genes and regulatory elements. Nature 423(6937):241-54
SGD Papers Entry  Pubmed Entry  DOI full text  Web Supplement  SGD Curated Comments & Errata
Cliften P, et al. (2003) Finding functional features in Saccharomyces genomes by phylogenetic footprinting. Science 301(5629):71-6
SGD Papers Entry  Pubmed Entry  DOI full text  Web Supplement  

2011-02-03CEN1, YAR002W152189152190SubstitutionCAAC
 A dinucleotide substitution was made in the intergenic region between CEN1 and ORF NUP60/YAR002W
New    152153  ATATATTTACAAGTGAAAGCTTATTGTAATGTGTACTTTTAAACATCAAATAACAGACCT  152212
               ||||||||||||||||||||||||||||||||||  ||||||||||||||||||||||||
Old    152155  ATATATTTACAAGTGAAAGCTTATTGTAATGTGTCATTTTAAACATCAAATAACAGACCT  152214

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03YAR007C, YAR008W158934158934Insertion G
 A single nucleotide insertion was made in the intergenic region between ORFs RFA1/YAR007C and SEN34/YAR008W.
New    158923  TAACATATATGCAAAAAGAACGGCAAAAGGCGAGGAGGTTTTTATGCCACCGCTAGTATT  158982
               |||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
Old    158925  TAACATATAT-CAAAAAGAACGGCAAAAGGCGAGGAGGTTTTTATGCCACCGCTAGTATT  158983

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2004-01-30YAR014C166772166772DeletionG
 Kellis et al. 2003 predicted and confirmed the deletion of a single G nucleotide at chromosomal coordinate 166772. As a consequence of this sequence change, YAR014C was extended at the 3' end, altering the C-terminus and increasing the size of the predicted protein from 702 to 707 amino acids.
New: 166731 TTGTAAGGACAATATCATTTACGAATAATTTCATCCAATTG-TTTCATCAACACA 166784
            ||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Old: 166731 TTGTAAGGACAATATCATTTACGAATAATTTCATCCAATTGGTTTCATCAACACA 166785 

Kellis M, et al. (2003) Sequencing and comparison of yeast species to identify genes and regulatory elements. Nature 423(6937):241-54
SGD Papers Entry  Pubmed Entry  DOI full text  Web Supplement  SGD Curated Comments & Errata

2011-02-03
YAR014C
167048167048SubstitutionGC
167084167084Insertion C
167113167113Insertion T
167133167133Insertion A
167138167138Insertion T
167142167142Insertion A
167149167149Insertion T
167551167551SubstitutionGC
167802167802SubstitutionCT
 Six separate single nucleotides were inserted within ORF BUD14/YAR014C, and three single nucleotide substitutions were also made, altering its coding sequence. The start, stop, and majority of the reading frame remain the same, but the annotated protein is now two amino acids longer and a small section of the protein sequence is now different.
New    167033  GTCAGTTTGATTTGCCATTCCTCCACTAGACCCCAAGCTGGCTTGAATTTGCCCCTCTGT  167092
               |||||||||||||| |||||||||||||||||||||||||||||||||||| ||||||||
Old    167034  GTCAGTTTGATTTGGCATTCCTCCACTAGACCCCAAGCTGGCTTGAATTTG-CCCTCTGT  167092

New    167093  GTTTTCCACGTTTGTTGTCTCTTCAATTTTCGATTTTGCATGAATATATTGTGAAATGTC  167152
               ||||||||||||||||||||| |||||||||||||||||||| ||||| |||| ||||||
Old    167093  GTTTTCCACGTTTGTTGTCTC-TCAATTTTCGATTTTGCATG-ATATA-TGTG-AATGTC  167148

New    167153  CTTTATTGCTCTAGATGATGGAATACTGCTGCTTTCTTCAACTATTGGAGTGGATGGTGA  167212
               | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Old    167149  C-TTATTGCTCTAGATGATGGAATACTGCTGCTTTCTTCAACTATTGGAGTGGATGGTGA  167207

New    167513  GACGTCGCTCACTACATCGCTCGTATCATCATCCTGGAATTTGCCAAAATTTAACTTCGC  167572
               ||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
Old    167508  GACGTCGCTCACTACATCGCTCGTATCATCATCCTGGAATTTGGCAAAATTTAACTTCGC  167567

New    167763  TATCCATTCAGTGCAGGTGTCGGAATTATACTCTCTGCATCACTTTGATTCTTGTTACCA  167822
               |||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
Old    167758  TATCCATTCAGTGCAGGTGTCGGAATTATACTCTCTGCATCACTCTGATTCTTGTTACCA  167817

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03
YAR018C, YAR019C
171882171882Insertion C
171968171968DeletionC
171972171972SubstitutionCT
171975171977DeletionCTA
171983171983DeletionG
172018172018DeletionA
172020172020DeletionA
172041172041Insertion T
172042172042Insertion T
172170172170Insertion T
 Several nucleotide sequence changes were made in the intergenic region between ORFs KIN3/YAR018C and CDC15/YAR019C.
New    171843  GTAATGAAAAAATTCAGAAACCTTAAAAAAAAAACTTGGCTGTAACCTATCGGAAGACTG  171902
               ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
Old    171838  GTAATGAAAAAATTCAGAAACCTTAAAAAAAAAACTTGGCTGTAA-CTATCGGAAGACTG  171896
New    171953  CTAGAAACATTTAACAAAATG-CACTTT---GAGTC-GTTCATACATTTAATCCCCAATT  172007
               ||||||||||||||||||||| ||| ||   ||||| |||||||||||||||||||||||
Old    171947  CTAGAAACATTTAACAAAATGCCACCTTCTAGAGTCGGTTCATACATTTAATCCCCAATT  172006
New    172008  GAAAAAAAAAA-G-AAAAGAAAAAAAGCATATATATGTATATGCTTTTTTATCATTACTG  172065
               ||||||||||| | ||||||||||||||||||||| | ||||||||||||||||||||||
Old    172007  GAAAAAAAAAAAGAAAAAGAAAAAAAGCATATATA-G-ATATGCTTTTTTATCATTACTG  172064
New    172126  GGAAGGTTCACAATTCTATATATAGTGTTAATGTAATGCTGTATTATTTCTCTATATATG  172185
               |||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Old    172125  GGAAGGTTCACAATTCTATATATAGTGTTAATGTAATGCTGTATTA-TTCTCTATATATG  172183

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03
YAR019C
172425172425Insertion G
172434172434DeletionG
174173174173SubstitutionGC
174187174188SubstitutionCGGC
 Nucleotide changes within the coding region of CDC15/YAR019C resulted in an altered protein sequence. The start, stop, and reading frame remain the same, but protein residue 316 is now Alanine rather than Arginine, residue 321 is now Alanine rather than Proline, and 900-902 are now KDV rather than NGC.
New    172416  GAATTCCTTTTTGGGACATCC-TTGTTCCAGTTGTTGTTTAAAAACTCCGTTATTTGTAC  172474
               |||||||||||| |||||||| ||||||||||||||||||||||||||||||||||||||
Old    172414  GAATTCCTTTTT-GGACATCCGTTGTTCCAGTTGTTGTTTAAAAACTCCGTTATTTGTAC  172472

New    174165  TCTGCCCAGGCAGCGGGAGCCGCTGCAAGACTGAATTTAGAGGGTGATATATTTAGTTTC  174224
               |||||||||| |||||||||||||  ||||||||||||||||||||||||||||||||||
Old    174163  TCTGCCCAGGGAGCGGGAGCCGCTCGAAGACTGAATTTAGAGGGTGATATATTTAGTTTC  174222

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03YAR019W-A175305175305Insertion C
 A single C nucleotide was inserted very near the 3' end of ORF YAR019W-A, altering its coding sequence. The start and most of the reading frame remain the same, but the C-terminus has changed and the annotated protein is now 4 amino acids shorter.
New    175295  GGCACAGCAAAGTCCGCACAGAGCACTACAGTATAGCATAGAGTGCTAATGAGTTGATAG  175354
               ||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
Old    175293  GGCACAGCAAAGT-CGCACAGAGCACTACAGTATAGCATAGAGTGCTAATGAGTTGATAG  175351

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03
ARS110, YAR019W-A
175371175371DeletionT
175378175378SubstitutionTC
 A single nucleotide deletion and a single nucleotide substitution were made in the intergenic region between ORF YAR019W-A and ARS110.
New    175355  GCCCAATTTTGATTATGCC-TCTTTTCCATACACGACGCCAGAGGACATTATTACATTAC  175413
               ||||||||||||||||||| |||||| |||||||||||||||||||||||||||||||||
Old    175352  GCCCAATTTTGATTATGCCTTCTTTTTCATACACGACGCCAGAGGACATTATTACATTAC  175411

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03
YAR020C, YAR023C
177135177135Insertion T
178256178256SubstitutionGC
178601178601DeletionA
178618178618Insertion A
178638178638DeletionG
178647178647SubstitutionTC
 Several nucleotide sequence changes were made in the intergenic region between ORFs PAU7/YAR020C and YAR023C.
New    177104  CAAAGCTGATCAAGCCGCTGTATTTATATGAAACTTTGAACAACTACATCTGCACACATG  177163
               |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
Old    177102  CAAAGCTGATCAAGCCGCTGTATTTATATGAAAC-TTGAACAACTACATCTGCACACATG  177160
New    178244  ACAATTTAACCATGGCAGGTTAAAATATTACTGCGATCAGTAAAAATGGGGATATCACCT  178303
               ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
Old    178241  ACAATTTAACCATGGGAGGTTAAAATATTACTGCGATCAGTAAAAATGGGGATATCACCT  178300
New    178599  ATTGT-AGATTGTATCGTTCGAGAAACGTCAGGCATGATAGAT-GTTGCAATCACAGGAC  178656
               ||||| ||||||||||||||||| ||||||||||||||||||| |||||||| |||||||
Old    178596  ATTGTAAGATTGTATCGTTCGAG-AACGTCAGGCATGATAGATGGTTGCAATTACAGGAC  178654

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03
YAR023C
179683179683SubstitutionTA
179761179761SubstitutionCA
 The substitution of two nucleotides within the coding region of YAR023C resulted in an altered protein sequence. The start, stop, and reading frame remain the same, but protein residue 20 is now Phenylalanine rather than Valine, and residue 46 is now Phenylalanine rather than Isoleucine.
New    179672  ATTGGTCTACTGAATGACCATATCTGAAGGACTACCATAGAGCCACCTAAACATATCCGG  179731
               ||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
Old    179670  ATTGGTCTACTGATTGACCATATCTGAAGGACTACCATAGAGCCACCTAAACATATCCGG  179729

New    179732  ATCACCATGGCAGGGGAGAGAACACCAGAAAACCAAATGTTTGTTAAAGTCGCCAAAATC  179791
               ||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
Old    179730  ATCACCATGGCAGGGGAGAGAACACCAGAAACCCAAATGTTTGTTAAAGTCGCCAAAATC  179789

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03YAR023C, tL(CAA)A180961180961Insertion CAAA
 A tetranucleotide insertion was made in the intergenic region between ORF YAR023C and tRNA-Leu SUP56/tL(CAA)A.
New    180942  TTCCCATTACAATGCCGAATAGCAAAATATGTAGTAGAACACGTACACGCATGATAATTA  181001
               ||||||||||||||||||||||    ||||||||||||||||||||||||||||||||||
Old    180940  TTCCCATTACAATGCCGAATAG----ATATGTAGTAGAACACGTACACGCATGATAATTA  180995

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2003-09-27YAR042W, YAR044W193300193300InsertionG
 Insertion of a single G after the G at 193300 merged SWH1/YAR042W and OSH1/YAR044W. After merging YAR042W (coordinates 192613-193383 (1-771)) and YAR044W (coordinates 193599-196178 (1-2580)), the coordinates of the merged ORF, SWH1/YAR042W, are 192613-196179 (1-3567). OSH1 and YAR044W are now aliases of SWH1/YAR042W. This sequence change was predicted by several studies, then verified in S288c by SGD.

Old: 193261 CTTGAACACGGTGCTGACCCCTTCAAGAGAGACCGCAAGG-CAAACTGCCCATCGAGCTC 193319
            |||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
New: 193261 CTTGAACACGGTGCTGACCCCTTCAAGAGAGACCGCAAGGGCAAACTGCCCATCGAGCTC 193320

Schmalix WA and Bandlow W (1994) SWH1 from yeast encodes a candidate nuclear factor containing ankyrin repeats and showing homology to mammalian oxysterol-binding protein. Biochim Biophys Acta 1219(1):205-10
SGD Papers Entry  Pubmed Entry  DOI full text  
Beh CT, et al. (2001) Overlapping functions of the yeast oxysterol-binding protein homologues. Genetics 157(3):1117-40
SGD Papers Entry  Pubmed Entry  PMC full text  
Kellis M, et al. (2003) Sequencing and comparison of yeast species to identify genes and regulatory elements. Nature 423(6937):241-54
SGD Papers Entry  Pubmed Entry  DOI full text  Web Supplement  SGD Curated Comments & Errata
Brachat S, et al. (2003) Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii. Genome Biol 4(7):R45
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  
Cliften P, et al. (2003) Finding functional features in Saccharomyces genomes by phylogenetic footprinting. Science 301(5629):71-6
SGD Papers Entry  Pubmed Entry  DOI full text  Web Supplement  

2006-01-19YAR042W193355193355SubstitutionGA
 When confirming the G nucleotide insertion that resulted in the merger of SWH1/YAR042W and OSH1/YAR044W (see 2003-09-27), SGD detected an additional sequencing error. The substitution of an A nt for a G nt changes amino acid 248 from a serine to an asparagine.
Substitute an A nt for the G nt at 193355
New: 193341 CGACACCGCCACCAACACCAAGATCGCCATC 193371
            |||||||||||||| ||||||||||||||||
Old: 193341 CGACACCGCCACCAGCACCAAGATCGCCATC 193371
2011-02-03YAR042W, YAR047C198900198900Insertion A
 A single nucleotide insertion was made in the intergenic region between ORFs SWH1/YAR042W and YAR047C.
New    198882  TAAGCCCCTTTTATGGATGCATGAGAAAAAAAAAAAGGTTTGCTACAACCATCTCAGGTC  198941
               ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
Old    198876  TAAGCCCCTTTTATGGATGCATGAG-AAAAAAAAAAGGTTTGCTACAACCATCTCAGGTC  198934

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

2011-02-03
YAR047C, YAR050W
202933202933Insertion A
203327203327Insertion T
 Two separate single nucleotide insertions were made in the intergenic region between ORFs YAR047C and FLO1/YAR050W.
New    202902  GCTCATTAATTGCCCTCACAAGAATTTGGAAGTGCGTAGAACAGGTAAAAGATTGTACTA  202961
               ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
Old    202895  GCTCATTAATTGCCCTCACAAGAATTTGGAAGTGCGTAG-ACAGGTAAAAGATTGTACTA  202953
New    203322  TTCACTCTTGCTCGTTTGATGTAAGCTCTCTTCCGGGTTCTTATTTTTAATTCTTGTCAC  203381
               |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
Old    203314  TTCACTCTTGCTCG-TTGATGTAAGCTCTCTTCCGGGTTCTTATTTTTAATTCTTGTCAC  203372

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

1998-09-02YAR050W203939203939SubstitutionTG
 The following sequence change was made to the systematic sequence of Chromosome I within the ORF YAR050W/FLO1: transversion of T to G at 203939.
Old: 203881 CAATTCTATCAGTAGGTGGTGCAACCGCGTTCAACTGTTGTGCTCAACAGCAACCGCCTA 203940
            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
New: 203876 CAATTCTATCAGTAGGTGGTGCAACCGCGTTCAACTGTTGTGCTCAACAGCAACCGCCGA 203935
1998-09-02
YAR060C, YARCdelta8
210804210804DeletionT
210810210810SubstitutionTA
211011211011SubstitutionCT
 The following changes were made to the systematic sequence of Chromosome I in the region encompassing features YARCdelta8 and YAR060C: deletion of the T at 210804, transversion of T to A at 210810, and transition of C to T at 211011.
Old: 210781 TTTCTCTTTGCTTGCATCTTTTTTCTTCCTTGCCAAAAAATAAAAGATACTCATTTTAAA 210840
            ||||||||||||||||||||||| ||||| ||||||||||||||||||||||||||||||
New: 210776 TTTCTCTTTGCTTGCATCTTTTT-CTTCCATGCCAAAAAATAAAAGATACTCATTTTAAA 210834
Old: 210961 GCCCTTCTACGGCTTCTTCTAACCAATTGTTCCCCGTGAGTTGCTTTCTCCGAAAACCTT 211020
            |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
New: 210955 GCCCTTCTACGGCTTCTTCTAACCAATTGTTCCCCGTGAGTTGCTTTCTCTGAAAACCTT 211014
2011-02-03TEL01R, TEL01R-XC229568229568Insertion G
 A single nucleotide insertion was made within the right telomere of Chromosome 1, TEL01R, specifically within the X element Core sequence TEL01R-XC.
New    229542  ATTACGCATGGAGTTAAGAGTATTTACATGATAATTGGGGTTCCGTGATTCATTATAGAT  229601
               |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
Old    229533  ATTACGCATGGAGTTAAGAGTATTTACATGATAATT-GGGTTCCGTGATTCATTATAGAT  229591

Engel SR, et al. (2013) The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now. G3 (Bethesda)
SGD Papers Entry  Pubmed Entry  DOI full text  PMC full text  

ANNOTATION CHANGES without sequence changesJump to: Sequence changes

Date Affected Features
2007-05-08snR18
 Updated coordinates of snR18 based on GenBank U12981.

Hiraga K, et al. (1993) Cloning and characterization of the elongation factor EF-1 beta homologue of Saccharomyces cerevisiae. EF-1 beta is essential for growth. FEBS Lett 316(2):165-9
SGD Papers Entry  Pubmed Entry  DOI full text  

2007-05-08HRA1
 Updated coordinates of HRA1 based on Yang & Altman 2007.

Yang L and Altman S (2007) A noncoding RNA in Saccharomyces cerevisiae is an RNase P substrate. RNA 13(5):682-90
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  

2007-03-07ARS102, ARS103, ARS105, ARS108, ARS111, ARS112
 The following ARS elements were added to the genome annotation on Chromosome I based on Xu et al. 2006: ARS102, ARS103, ARS105, ARS108, ARS111, ARS112.

Xu W, et al. (2006) Genome-wide mapping of ORC and Mcm2p binding sites on tiling arrays and identification of essential ARS consensus sequences in S. cerevisiae. BMC Genomics 7():276
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  

2007-03-07ARS109
 The ACS within ARS101/ARS109 at coordinates 159946-159936 was added based on Xu et al. 2006 and Theis & Newlon 2001.

Theis JF and Newlon CS (2001) Two compound replication origins in Saccharomyces cerevisiae contain redundant origin recognition complex binding sites. Mol Cell Biol 21(8):2790-801
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  
Xu W, et al. (2006) Genome-wide mapping of ORC and Mcm2p binding sites on tiling arrays and identification of essential ARS consensus sequences in S. cerevisiae. BMC Genomics 7():276
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  

2007-02-06HRA1
 This RNA gene, HRA1, was described by Samanta et al. (2006). Approximate start and stop coordinates, which are accurate to within 25 nucleotides, have been specified for this non-coding RNA. Many thanks to Manoj Samanta for alerting us to this noncoding RNA.

Samanta MP, et al. (2006) Global identification of noncoding RNAs in Saccharomyces cerevisiae by modulating an essential RNA processing pathway. Proc Natl Acad Sci U S A 103(11):4192-7
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  yfgdb  

2006-10-02ARS109
 An ARS Consensus Sequence (ACS) was added to ARS109 based on Nieduszynski et al. 2006.

Nieduszynski CA, et al. (2006) Genome-wide identification of replication origins in yeast by comparative genomics. Genes Dev 20(14):1874-9
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  Web Supplement  

2006-09-06ARS104, ARS106, ARS107
 The following ARS elements on Chromosome I were added to SGD based on Nieduszynski et al. 2006: ARS104, ARS106, ARS107.

Nieduszynski CA, et al. (2006) Genome-wide identification of replication origins in yeast by comparative genomics. Genes Dev 20(14):1874-9
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  Web Supplement  

2006-09-06ARS109, ARS110
 Updated coordinates of the following ARS elements on Chromosome I based on Nieduszynski et al. 2006: ARS101/109, ARS110.

Nieduszynski CA, et al. (2006) Genome-wide identification of replication origins in yeast by comparative genomics. Genes Dev 20(14):1874-9
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  Web Supplement  

2006-05-08CEN1
 The previously annotated boundaries of CEN1 were adjusted to coincide with the 5' end of CDEI and the 3' end of CDEIII, to more accurately reflect current knowledge regarding centromere structure in Saccharomyces cerevisiae.

Wieland G, et al. (2001) Determination of the binding constants of the centromere protein Cbf1 to all 16 centromere DNAs of Saccharomyces cerevisiae. Nucleic Acids Res 29(5):1054-60
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  
Espelin CW, et al. (2003) Binding of the essential Saccharomyces cerevisiae kinetochore protein Ndc10p to CDEII. Mol Biol Cell 14(11):4557-68
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  

2006-02-28ARS110
 This ARS element ARS110 was added to the genome annotation based on Wyrick et al. 2001 and Dimock et al. 1984.

Dimock K, et al. (1984) Molecular cloning of the ADE1 gene of Saccharomyces cerevisiae and stability of the transformants. Gene 27(2):233-7
SGD Papers Entry  Pubmed Entry  DOI full text  
Wyrick JJ, et al. (2001) Genome-wide distribution of ORC and MCM proteins in S. cerevisiae: high-resolution mapping of replication origins. Science 294(5550):2357-60
SGD Papers Entry  Pubmed Entry  DOI full text  yfgdb  SGD Curated Comments & Errata

2004-10-18ARS109
 ARS101 was added to SGD based on Theis and Newlon 2001 and Wyrick et al. 2001.

Theis JF and Newlon CS (2001) Two compound replication origins in Saccharomyces cerevisiae contain redundant origin recognition complex binding sites. Mol Cell Biol 21(8):2790-801
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  
Wyrick JJ, et al. (2001) Genome-wide distribution of ORC and MCM proteins in S. cerevisiae: high-resolution mapping of replication origins. Science 294(5550):2357-60
SGD Papers Entry  Pubmed Entry  DOI full text  yfgdb  SGD Curated Comments & Errata

2004-10-12CEN1
 Centromeric DNA elements CDEI, CDEII, and CDEIII were annotated based on Wieland et al. 2001 and Espelin et al. 2003.

Wieland G, et al. (2001) Determination of the binding constants of the centromere protein Cbf1 to all 16 centromere DNAs of Saccharomyces cerevisiae. Nucleic Acids Res 29(5):1054-60
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  
Espelin CW, et al. (2003) Binding of the essential Saccharomyces cerevisiae kinetochore protein Ndc10p to CDEII. Mol Biol Cell 14(11):4557-68
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  

2003-09-22YAL044C
 The start site of GCV3/YAL044C was moved 21 nt (7 codons) downstream, based on the automated comparison of closely-related Saccharomyces species by Kellis et al. 2003. The numbering for both the nucleotides in the DNA coding sequence and the amino acids in the predicted protein have been updated. Evidence supporting this change includes: 1) This is the predicted start methionine in the majority of Saccharomyces species orthologs analyzed by Kellis et al. and/or Cliften et al.; 2) Significant sequence conservation begins abruptly at this predicted start methionine.

Kellis M, et al. (2003) Sequencing and comparison of yeast species to identify genes and regulatory elements. Nature 423(6937):241-54
SGD Papers Entry  Pubmed Entry  DOI full text  Web Supplement  SGD Curated Comments & Errata
Cliften P, et al. (2003) Finding functional features in Saccharomyces genomes by phylogenetic footprinting. Science 301(5629):71-6
SGD Papers Entry  Pubmed Entry  DOI full text  Web Supplement  

2003-09-22YAL034C
 The start site of FUN19/YAL034C was moved 150 nt (50 codons) downstream, based on the automated comparison of closely-related Saccharomyces species by Kellis et al. 2003. The numbering for both the nucleotides in the DNA coding sequence and the amino acids in the predicted protein have been updated. Evidence supporting this change includes: 1) This is the predicted start methionine in the majority of Saccharomyces species orthologs analyzed by Kellis et al. and/or Cliften et al.; 2) Significant sequence conservation begins abruptly at this predicted start methionine.

Kellis M, et al. (2003) Sequencing and comparison of yeast species to identify genes and regulatory elements. Nature 423(6937):241-54
SGD Papers Entry  Pubmed Entry  DOI full text  Web Supplement  SGD Curated Comments & Errata
Cliften P, et al. (2003) Finding functional features in Saccharomyces genomes by phylogenetic footprinting. Science 301(5629):71-6
SGD Papers Entry  Pubmed Entry  DOI full text  Web Supplement  

2003-09-22YAL011W
 The start site of YAL011W was moved 39 nt (13 codons) downstream, based on the automated comparison of closely-related Saccharomyces species by Kellis et al. 2003. The numbering for both the nucleotides in the DNA coding sequence and the amino acids in the predicted protein have been updated. Evidence supporting this change includes: 1) This is the predicted start methionine in the majority of Saccharomyces species orthologs analyzed by Kellis et al. and/or Cliften et al.; 2) Significant sequence conservation begins abruptly at this predicted start methionine.

Kellis M, et al. (2003) Sequencing and comparison of yeast species to identify genes and regulatory elements. Nature 423(6937):241-54
SGD Papers Entry  Pubmed Entry  DOI full text  Web Supplement  SGD Curated Comments & Errata
Cliften P, et al. (2003) Finding functional features in Saccharomyces genomes by phylogenetic footprinting. Science 301(5629):71-6
SGD Papers Entry  Pubmed Entry  DOI full text  Web Supplement  

2003-09-09TEL01L, TEL01L-TR, TEL01L-XC, TEL01L-XR, TEL01R, TEL01R-TR, TEL01R-XC
 The chromosomal locations for the following telomeric features on Chromosome I were generously provided by Ed Louis and Dave Barton (University of Leicester, UK): TEL01L, TEL01L-TR, TEL01L-XC, TEL01L-XR, TEL01R, TEL01R-TR, and TEL01R-XC.
2003-07-29YAL037C-B, YAL067W-A, YAL068W-A, YAR035C-A
 Thanks to Kumar et al. 2002 for providing the coordinates of the following ORFs on Chromosome I: YAL037C-B, YAL067W-A, YAL068W-A, YAR035C-A.

Kumar A, et al. (2002) An integrated approach for finding overlooked genes in yeast. Nat Biotechnol 20(1):58-63
SGD Papers Entry  Pubmed Entry  DOI full text  Web Supplement  yfgdb  SGD Curated Comments & Errata

2003-07-29YAL063C-A
 Thanks to Oshiro et al., Velculescu et al., and Basrai et al. for providing the coordinates of ORF YAL063C-A on Chromosome I.

Basrai MA, et al. (1999) NORF5/HUG1 is a component of the MEC1-mediated checkpoint response to DNA damage and replication arrest in Saccharomyces cerevisiae. Mol Cell Biol 19(10):7041-9
SGD Papers Entry  Pubmed Entry  PMC full text  
Velculescu VE, et al. (1997) Characterization of the yeast transcriptome. Cell 88(2):243-51
SGD Papers Entry  Pubmed Entry  DOI full text  yfgdb  
Oshiro G, et al. (2002) Parallel identification of new genes in Saccharomyces cerevisiae. Genome Res 12(8):1210-20
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  Web Supplement  yfgdb  

2003-07-29YAL016C-A, YAL019W-A, YAL026C-A, YAL031W-A, YAL037C-A, YAL047W-A, YAL059C-A, YAR019W-A
 Thanks to MIPS for providing the coordinates of the following ORFs on Chromosome I: YAL016C-A, YAL019W-A, YAL026C-A, YAL031W-A, YAL037C-A, YAL047W-A, YAL059C-A, and YAR019W-A.
2003-07-29YAL016C-B
 Thanks to Kessler et al. 2003 for providing the coordinates of ORF YAL016C-B on Chromosome I.

Kessler MM, et al. (2003) Systematic discovery of new genes in the Saccharomyces cerevisiae genome. Genome Res 13(2):264-71
SGD Papers Entry  Pubmed Entry  PMC full text  DOI full text  

2001-01-30YAL044W-A
 ORF added based on similarity to an S. pombe gene (information submitted by Valerie Wood).
1999-07-17YAR043C, YAR052C, YAR074C
 Deleted ORF, does not encode a protein; this putative ORF was included in the original annotation of Chromosome I but was later withdrawn

Jump to: Sequence Changes | Annotation Changes without Sequence Changes