Ontology: Molecular Function (GO:0008301)

Definition: The activity of binding selectively and non-covalently to and distorting the original structure of DNA, typically a straight helix, into a bend, or increasing the bend if the original structure was intrinsically bent due to its sequence.

Synonyms: DNA bending activity; DNA bending involving DNA binding

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term DNA binding, bending (15 genes) or to its variants containing one or more qualifiers (8 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	contributes_to: DNA binding, bending	8
	DNA binding, bending	15
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term DNA binding, bending or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

contributes_to: DNA binding, bending      8 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
HMLALPHA2/YCL067C	ISA: Inferred from Sequence Alignment with SGD:MATALPHA2 Assigned on 2013-04-29	manually curated	Astell CR, et al.  (1981) The sequence of the DNAs coding for the mating-type loci of Saccharomyces cerevisiae. Cell 27(1 Pt 2):15-23	SGD
MATA1	IDA: Inferred from Direct Assay Assigned on 2006-11-27	manually curated	Li T, et al.  (1995) Crystal structure of the MATa1/MAT alpha 2 homeodomain heterodimer bound to DNA. Science 270(5234):262-9	SGD
	IDA: Inferred from Direct Assay Assigned on 2006-11-27	manually curated	Smith DL, et al.  (1995) DNA bending by the a1 and alpha 2 homeodomain proteins from yeast. Nucleic Acids Res 23(7):1239-43	SGD
MATALPHA2/YCR039C	IDA: Inferred from Direct Assay Assigned on 2006-11-27	manually curated	Li T, et al.  (1995) Crystal structure of the MATa1/MAT alpha 2 homeodomain heterodimer bound to DNA. Science 270(5234):262-9	SGD
	IDA: Inferred from Direct Assay Assigned on 2006-11-27	manually curated	Smith DL, et al.  (1995) DNA bending by the a1 and alpha 2 homeodomain proteins from yeast. Nucleic Acids Res 23(7):1239-43	SGD
TFC1/YBR123C	IDA: Inferred from Direct Assay Assigned on 2012-01-05	manually curated	Leveillard T, et al.  (1991) Saccharomyces cerevisiae transcription factors IIIB and IIIC bend the DNA of a tRNA(Gln) gene. J Biol Chem 266(8):5162-8	SGD
TFC3/YAL001C	IDA: Inferred from Direct Assay Assigned on 2012-01-05	manually curated	Leveillard T, et al.  (1991) Saccharomyces cerevisiae transcription factors IIIB and IIIC bend the DNA of a tRNA(Gln) gene. J Biol Chem 266(8):5162-8	SGD
TFC4/YGR047C	IDA: Inferred from Direct Assay Assigned on 2012-01-05	manually curated	Leveillard T, et al.  (1991) Saccharomyces cerevisiae transcription factors IIIB and IIIC bend the DNA of a tRNA(Gln) gene. J Biol Chem 266(8):5162-8	SGD
TFC7/YOR110W	IDA: Inferred from Direct Assay Assigned on 2012-01-05	manually curated	Leveillard T, et al.  (1991) Saccharomyces cerevisiae transcription factors IIIB and IIIC bend the DNA of a tRNA(Gln) gene. J Biol Chem 266(8):5162-8	SGD
TFC8/YPL007C	IDA: Inferred from Direct Assay Assigned on 2012-01-05	manually curated	Leveillard T, et al.  (1991) Saccharomyces cerevisiae transcription factors IIIB and IIIC bend the DNA of a tRNA(Gln) gene. J Biol Chem 266(8):5162-8	SGD

DNA binding, bending      15 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
ABF2/YMR072W	IDA: Inferred from Direct Assay Assigned on 2004-07-20	manually curated	Fisher RP, et al.  (1992) DNA wrapping and bending by a mitochondrial high mobility group-like transcriptional activator protein. J Biol Chem 267(5):3358-67	SGD
CBF2/YGR140W	IDA: Inferred from Direct Assay Assigned on 2002-10-17	manually curated	Pietrasanta LI, et al.  (1999) Probing the Saccharomyces cerevisiae centromeric DNA (CEN DNA)-binding factor 3 (CBF3) kinetochore complex by using atomic force microscopy. Proc Natl Acad Sci U S A 96(7):3757-62	SGD
CEP3/YMR168C	IDA: Inferred from Direct Assay Assigned on 2002-10-17	manually curated	Pietrasanta LI, et al.  (1999) Probing the Saccharomyces cerevisiae centromeric DNA (CEN DNA)-binding factor 3 (CBF3) kinetochore complex by using atomic force microscopy. Proc Natl Acad Sci U S A 96(7):3757-62	SGD
CTF13/YMR094W	IDA: Inferred from Direct Assay Assigned on 2002-10-17	manually curated	Pietrasanta LI, et al.  (1999) Probing the Saccharomyces cerevisiae centromeric DNA (CEN DNA)-binding factor 3 (CBF3) kinetochore complex by using atomic force microscopy. Proc Natl Acad Sci U S A 96(7):3757-62	SGD
FLP1/R0010W	IMP: Inferred from Mutant Phenotype Assigned on 2006-08-04	manually curated	Schwartz CJ and Sadowski PD  (1989) FLP recombinase of the 2 microns circle plasmid of Saccharomyces cerevisiae bends its DNA target. Isolation of FLP mutants defective in DNA bending. J Mol Biol 205(4):647-58	SGD
HMLALPHA1/YCL066W	ISA: Inferred from Sequence Alignment with SGD:MATALPHA1 Assigned on 2013-04-29	manually curated	Astell CR, et al.  (1981) The sequence of the DNAs coding for the mating-type loci of Saccharomyces cerevisiae. Cell 27(1 Pt 2):15-23	SGD
HMO1/YDR174W	IDA: Inferred from Direct Assay Assigned on 2010-03-17	manually curated	Kamau E, et al.  (2004) The Saccharomyces cerevisiae high mobility group box protein HMO1 contains two functional DNA binding domains. J Biol Chem 279(53):55234-40	SGD
	IDA: Inferred from Direct Assay Assigned on 2009-04-29	manually curated	Bauerle KT, et al.  (2006) Interactions between N- and C-terminal domains of the Saccharomyces cerevisiae high-mobility group protein HMO1 are required for DNA bending. Biochemistry 45(11):3635-45	SGD
MATALPHA1/YCR040W	IDA: Inferred from Direct Assay Assigned on 2009-04-21	manually curated	Carr EA, et al.  (2004) Alpha1-induced DNA bending is required for transcriptional activation by the Mcm1-alpha1 complex. Nucleic Acids Res 32(8):2298-305	SGD
NHP6A/YPR052C	IDA: Inferred from Direct Assay Assigned on 2010-03-11	manually curated	Paull TT and Johnson RC  (1995) DNA looping by Saccharomyces cerevisiae high mobility group proteins NHP6A/B. Consequences for nucleoprotein complex assembly and chromatin condensation. J Biol Chem 270(15):8744-54	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-03-11	manually curated	Yen YM, et al.  (1998) Determinants of DNA binding and bending by the Saccharomyces cerevisiae high mobility group protein NHP6A that are important for its biological activities. Role of the unique N terminus and putative intercalating methionine. J Biol Chem 273(8):4424-35	SGD
NHP6B/YBR089C-A	IDA: Inferred from Direct Assay Assigned on 2010-03-11	manually curated	Paull TT and Johnson RC  (1995) DNA looping by Saccharomyces cerevisiae high mobility group proteins NHP6A/B. Consequences for nucleoprotein complex assembly and chromatin condensation. J Biol Chem 270(15):8744-54	SGD
RAP1/YNL216W	IDA: Inferred from Direct Assay Assigned on 2009-04-09	manually curated	Vignais ML and Sentenac A  (1989) Asymmetric DNA bending induced by the yeast multifunctional factor TUF. J Biol Chem 264(15):8463-6	SGD
RLM1/YPL089C	IDA: Inferred from Direct Assay Assigned on 2002-08-27	manually curated	West AG and Sharrocks AD  (1999) MADS-box transcription factors adopt alternative mechanisms for bending DNA. J Mol Biol 286(5):1311-23	SGD
ROX1/YPR065W	IDA: Inferred from Direct Assay Assigned on 2010-02-10	manually curated	Deckert J, et al.  (1999) Characterization of the DNA binding and bending HMG domain of the yeast hypoxic repressor Rox1. Nucleic Acids Res 27(17):3518-26	SGD
	IDA: Inferred from Direct Assay Assigned on 2002-08-06	manually curated	Deckert J, et al.  (1995) Mutational analysis of Rox1, a DNA-bending repressor of hypoxic genes in Saccharomyces cerevisiae. Mol Cell Biol 15(11):6109-17	SGD
SMP1/YBR182C	IDA: Inferred from Direct Assay Assigned on 2002-08-27	manually curated	West AG and Sharrocks AD  (1999) MADS-box transcription factors adopt alternative mechanisms for bending DNA. J Mol Biol 286(5):1311-23	SGD
SPT15/YER148W	IDA: Inferred from Direct Assay Assigned on 2009-08-31	manually curated	Horikoshi M, et al.  (1992) Transcription factor TFIID induces DNA bending upon binding to the TATA element. Proc Natl Acad Sci U S A 89(3):1060-4	SGD

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