Ontology: Biological Process (GO:0007035)

Definition: Any process that reduces the pH of the vacuole, measured by the concentration of the hydrogen ion.

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term vacuolar acidification (26 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	vacuolar acidification	26
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term vacuolar acidification or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

vacuolar acidification      26 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
DBF2/YGR092W	IMP: Inferred from Mutant Phenotype Assigned on 2008-09-26	manually curated	Makrantoni V, et al.  (2007) A novel role for the yeast protein kinase Dbf2p in vacuolar H+-ATPase function and sorbic acid stress tolerance. Microbiology 153(Pt 12):4016-26	SGD
MEH1/YKR007W	IMP: Inferred from Mutant Phenotype Assigned on 2005-06-30	manually curated	Gao XD, et al.  (2005) ERS1 encodes a functional homologue of the human lysosomal cystine transporter. FEBS J 272(10):2497-511	SGD
NHX1/YDR456W	IMP: Inferred from Mutant Phenotype Assigned on 2001-06-26	manually curated	Nass R and Rao R  (1999) The yeast endosomal Na+/H+ exchanger, Nhx1, confers osmotolerance following acute hypertonic shock. Microbiology 145 ( Pt 11):3221-8	SGD
RAV1/YJR033C	IGI: Inferred from Genetic Interaction with SGD:RAV2, IMP: Inferred from Mutant Phenotype Assigned on 2005-05-27	manually curated	Seol JH, et al.  (2001) Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat Cell Biol 3(4):384-91	SGD
	IGI: Inferred from Genetic Interaction with SGD:RAV2, IMP: Inferred from Mutant Phenotype Assigned on 2005-05-27	manually curated	Smardon AM, et al.  (2002) The RAVE complex is essential for stable assembly of the yeast V-ATPase. J Biol Chem 277(16):13831-9	SGD
RAV2/YDR202C	IGI: Inferred from Genetic Interaction with SGD:RAV1, IMP: Inferred from Mutant Phenotype Assigned on 2005-10-25	manually curated	Seol JH, et al.  (2001) Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat Cell Biol 3(4):384-91	SGD
	IGI: Inferred from Genetic Interaction with SGD:RAV1, IMP: Inferred from Mutant Phenotype Assigned on 2005-10-25	manually curated	Smardon AM, et al.  (2002) The RAVE complex is essential for stable assembly of the yeast V-ATPase. J Biol Chem 277(16):13831-9	SGD
RRG1/YDR065W	IMP: Inferred from Mutant Phenotype Assigned on 2006-04-24	manually curated	Eide DJ, et al.  (2005) Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae. Genome Biol 6(9):R77	SGD
SKP1/YDR328C	IGI: Inferred from Genetic Interaction with SGD:RAV2, SGD:RAV1, IMP: Inferred from Mutant Phenotype Assigned on 2005-05-27	manually curated	Seol JH, et al.  (2001) Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat Cell Biol 3(4):384-91	SGD
STV1/YMR054W	IEP: Inferred from Expression Pattern, IGI: Inferred from Genetic Interaction, IMP: Inferred from Mutant Phenotype, ISS: Inferred from Sequence or structural Similarity with SGD:VPH1 Assigned on 2006-04-11	manually curated	Kawasaki-Nishi S, et al.  (2001) Yeast V-ATPase complexes containing different isoforms of the 100-kDa a-subunit differ in coupling efficiency and in vivo dissociation. J Biol Chem 276(21):17941-8	SGD
	IGI: Inferred from Genetic Interaction Assigned on 2006-04-11	manually curated	Manolson MF, et al.  (1994) STV1 gene encodes functional homologue of 95-kDa yeast vacuolar H(+)-ATPase subunit Vph1p. J Biol Chem 269(19):14064-74	SGD
VMA1/YDL185W	IMP: Inferred from Mutant Phenotype Assigned on 2008-09-26	manually curated	Makrantoni V, et al.  (2007) A novel role for the yeast protein kinase Dbf2p in vacuolar H+-ATPase function and sorbic acid stress tolerance. Microbiology 153(Pt 12):4016-26	SGD
VMA10/YHR039C-A	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VMA11/YPL234C	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VMA13/YPR036W	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VMA16/YHR026W	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VMA2/YBR127C	IMP: Inferred from Mutant Phenotype Assigned on 2006-10-16	manually curated	Yamashiro CT, et al.  (1990) Role of vacuolar acidification in protein sorting and zymogen activation: a genetic analysis of the yeast vacuolar proton-translocating ATPase. Mol Cell Biol 10(7):3737-49	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2008-09-26	manually curated	Makrantoni V, et al.  (2007) A novel role for the yeast protein kinase Dbf2p in vacuolar H+-ATPase function and sorbic acid stress tolerance. Microbiology 153(Pt 12):4016-26	SGD
VMA22/YHR060W	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Preston RA, et al.  (1992) Genes required for vacuolar acidity in Saccharomyces cerevisiae. Genetics 131(3):551-8	SGD
VMA3/YEL027W	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VMA4/YOR332W	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VMA5/YKL080W	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VMA6/YLR447C	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VMA7/YGR020C	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VMA8/YEL051W	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VMA9/YCL005W-A	IMP: Inferred from Mutant Phenotype Assigned on 2004-04-27	manually curated	Sambade M and Kane PM  (2004) The yeast vacuolar proton-translocating ATPase contains a subunit homologous to the Manduca sexta and bovine e subunits that is essential for function. J Biol Chem 279(17):17361-5	SGD
VPH1/YOR270C	IMP: Inferred from Mutant Phenotype Assigned on 2006-10-19	manually curated	Manolson MF, et al.  (1992) The VPH1 gene encodes a 95-kDa integral membrane polypeptide required for in vivo assembly and activity of the yeast vacuolar H(+)-ATPase. J Biol Chem 267(20):14294-303	SGD
VPH2/YKL119C	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Preston RA, et al.  (1992) Genes required for vacuolar acidity in Saccharomyces cerevisiae. Genetics 131(3):551-8	SGD
VPS3/YDR495C	IMP: Inferred from Mutant Phenotype Assigned on 2005-05-31	manually curated	Raymond CK, et al.  (1990) Molecular analysis of the yeast VPS3 gene and the role of its product in vacuolar protein sorting and vacuolar segregation during the cell cycle. J Cell Biol 111(3):877-92	SGD
VPS45/YGL095C	IMP: Inferred from Mutant Phenotype Assigned on 2010-05-21	manually curated	Yoshida S, et al.  (1995) STT10, a novel class-D VPS yeast gene required for osmotic integrity related to the PKC1/STT1 protein kinase pathway. Gene 160(1):117-22	SGD

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