Ontology: Biological Process (GO:0006696)

Definition: The chemical reactions and pathways resulting in the formation of ergosterol, (22E)-ergosta-5,7,22-trien-3-beta-ol, a sterol found in ergot, yeast and moulds.

Synonyms: ergosterol anabolism; ergosterol biosynthesis; ergosterol formation; ergosterol synthesis

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term ergosterol biosynthetic process (23 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	ergosterol biosynthetic process	23
High-throughput	none	none
Computational (download data)	ergosterol biosynthetic process	1

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term ergosterol biosynthetic process or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

ergosterol biosynthetic process      23 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
ERG1/YGR175C	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-17	manually curated	Karst F and Lacroute F  (1977) Ertosterol biosynthesis in Saccharomyces cerevisiae: mutants deficient in the early steps of the pathway. Mol Gen Genet 154(3):269-77	SGD
ERG10/YPL028W	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-17	manually curated	Karst F and Lacroute F  (1977) Ertosterol biosynthesis in Saccharomyces cerevisiae: mutants deficient in the early steps of the pathway. Mol Gen Genet 154(3):269-77	SGD
ERG11/YHR007C	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-17	manually curated	Karst F and Lacroute F  (1977) Ertosterol biosynthesis in Saccharomyces cerevisiae: mutants deficient in the early steps of the pathway. Mol Gen Genet 154(3):269-77	SGD
ERG12/YMR208W	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-17	manually curated	Karst F and Lacroute F  (1977) Ertosterol biosynthesis in Saccharomyces cerevisiae: mutants deficient in the early steps of the pathway. Mol Gen Genet 154(3):269-77	SGD
ERG13/YML126C	IMP: Inferred from Mutant Phenotype Assigned on 2006-06-05	manually curated	Servouse M, et al.  (1984) Isolation and characterization of yeast mutants blocked in mevalonic acid formation. Biochem Biophys Res Commun 123(2):424-30	SGD
ERG2/YMR202W	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-14	manually curated	Rahier A, et al.  (2008) Identification of essential amino acid residues in a sterol 8,7-isomerase from Zea mays reveals functional homology and diversity with the isomerases of animal and fungal origin. Biochem J 414(2):247-59	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR006716 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
ERG20/YJL167W	IMP: Inferred from Mutant Phenotype Assigned on 2013-04-11	manually curated	Chambon C, et al.  (1991) Sterol pathway in yeast. Identification and properties of mutant strains defective in mevalonate diphosphate decarboxylase and farnesyl diphosphate synthetase. Lipids 26(8):633-6	SGD
ERG24/YNL280C	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-24	manually curated	Marcireau C, et al.  (1992) Construction and growth properties of a yeast strain defective in sterol 14-reductase. Curr Genet 22(4):267-72	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-24	manually curated	Lorenz RT and Parks LW  (1992) Cloning, sequencing, and disruption of the gene encoding sterol C-14 reductase in Saccharomyces cerevisiae. DNA Cell Biol 11(9):685-92	SGD
ERG25/YGR060W	IDA: Inferred from Direct Assay Assigned on 2001-01-18	manually curated	Li L and Kaplan J  (1996) Characterization of yeast methyl sterol oxidase (ERG25) and identification of a human homologue. J Biol Chem 271(28):16927-33	SGD
ERG26/YGL001C	IDA: Inferred from Direct Assay Assigned on 2001-01-18	manually curated	Gachotte D, et al.  (1998) Characterization of the Saccharomyces cerevisiae ERG26 gene encoding the C-3 sterol dehydrogenase (C-4 decarboxylase) involved in sterol biosynthesis. Proc Natl Acad Sci U S A 95(23):13794-9	SGD
ERG27/YLR100W	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Gachotte D, et al.  (1999) Characterization of the Saccharomyces cerevisiae ERG27 gene encoding the 3-keto reductase involved in C-4 sterol demethylation. Proc Natl Acad Sci U S A 96(22):12655-60	SGD
ERG28/YER044C	IDA: Inferred from Direct Assay Assigned on 2001-04-26	manually curated	Gachotte D, et al.  (2001) A novel gene conserved from yeast to humans is involved in sterol biosynthesis. J Lipid Res 42(1):150-4	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2001-04-26	manually curated	Hughes TR, et al.  (2000) Functional discovery via a compendium of expression profiles. Cell 102(1):109-26	SGD
	IPI: Inferred from Physical Interaction Assigned on 2005-07-11	manually curated	Mo C and Bard M  (2005) Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex. J Lipid Res 46(9):1991-8	SGD
ERG3/YLR056W	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-23	manually curated	Arthington BA, et al.  (1991) Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase. Gene 102(1):39-44	SGD
ERG4/YGL012W	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-22	manually curated	Lai MH, et al.  (1994) The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway. Gene 140(1):41-9	SGD
ERG5/YMR015C	IDA: Inferred from Direct Assay Assigned on 2010-06-23	manually curated	Kelly SL, et al.  (1995) Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61, a sterol delta 22-desaturase. FEBS Lett 377(2):217-20	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-23	manually curated	Skaggs BA, et al.  (1996) Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis. Gene 169(1):105-9	SGD
ERG6/YML008C	IMP: Inferred from Mutant Phenotype Assigned on 2013-03-15	manually curated	McCammon MT, et al.  (1984) Sterol methylation in Saccharomyces cerevisiae. J Bacteriol 157(2):475-83	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2013-03-15	manually curated	Gaber RF, et al.  (1989) The yeast gene ERG6 is required for normal membrane function but is not essential for biosynthesis of the cell-cycle-sparking sterol. Mol Cell Biol 9(8):3447-56	SGD
ERG7/YHR072W	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-17	manually curated	Karst F and Lacroute F  (1977) Ertosterol biosynthesis in Saccharomyces cerevisiae: mutants deficient in the early steps of the pathway. Mol Gen Genet 154(3):269-77	SGD
ERG8/YMR220W	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-17	manually curated	Karst F and Lacroute F  (1977) Ertosterol biosynthesis in Saccharomyces cerevisiae: mutants deficient in the early steps of the pathway. Mol Gen Genet 154(3):269-77	SGD
ERG9/YHR190W	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-17	manually curated	Karst F and Lacroute F  (1977) Ertosterol biosynthesis in Saccharomyces cerevisiae: mutants deficient in the early steps of the pathway. Mol Gen Genet 154(3):269-77	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-22	manually curated	Fegueur M, et al.  (1991) Isolation and primary structure of the ERG9 gene of Saccharomyces cerevisiae encoding squalene synthetase. Curr Genet 20(5):365-72	SGD
HMG1/YML075C	IMP: Inferred from Mutant Phenotype Assigned on 2010-06-17	manually curated	Donald KA, et al.  (1997) Effects of overproduction of the catalytic domain of 3-hydroxy-3-methylglutaryl coenzyme A reductase on squalene synthesis in Saccharomyces cerevisiae. Appl Environ Microbiol 63(9):3341-4	SGD
HMG2/YLR450W	ISA: Inferred from Sequence Alignment with SGD:HMG1 Assigned on 2010-06-17	manually curated	Basson ME, et al.  (1986) Saccharomyces cerevisiae contains two functional genes encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Proc Natl Acad Sci U S A 83(15):5563-7	SGD
MCR1/YKL150W	IDA: Inferred from Direct Assay Assigned on 2002-09-26	manually curated	Lamb DC, et al.  (1999) Biodiversity of the P450 catalytic cycle: yeast cytochrome b5/NADH cytochrome b5 reductase complex efficiently drives the entire sterol 14-demethylation (CYP51) reaction. FEBS Lett 462(3):283-8	SGD
NCP1/YHR042W	IMP: Inferred from Mutant Phenotype Assigned on 2002-04-19	manually curated	Venkateswarlu K, et al.  (1998) The N-terminal membrane domain of yeast NADPH-cytochrome P450 (CYP) oxidoreductase is not required for catalytic activity in sterol biosynthesis or in reconstitution of CYP activity. J Biol Chem 273(8):4492-6	SGD

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