Ontology: Biological Process (GO:0006623)

Definition: The process of directing proteins towards the vacuole, usually using signals contained within the protein.

Synonyms: protein vacuolar targeting; protein-vacuolar targeting; protein-vacuole targeting; vacuolar protein sorting

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term protein targeting to vacuole (52 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	protein targeting to vacuole	38
High-throughput (download data)	protein targeting to vacuole	14
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term protein targeting to vacuole or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

protein targeting to vacuole      52 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
APL5/YPL195W	IMP: Inferred from Mutant Phenotype Assigned on 2012-10-21	manually curated	Berger AC, et al.  (2007) The subcellular localization of the Niemann-Pick Type C proteins depends on the adaptor complex AP-3. J Cell Sci 120(Pt 20):3640-52	SGD
APL6/YGR261C	IMP: Inferred from Mutant Phenotype Assigned on 2012-10-21	manually curated	Berger AC, et al.  (2007) The subcellular localization of the Niemann-Pick Type C proteins depends on the adaptor complex AP-3. J Cell Sci 120(Pt 20):3640-52	SGD
APM3/YBR288C	IMP: Inferred from Mutant Phenotype Assigned on 2012-10-21	manually curated	Berger AC, et al.  (2007) The subcellular localization of the Niemann-Pick Type C proteins depends on the adaptor complex AP-3. J Cell Sci 120(Pt 20):3640-52	SGD
APS3/YJL024C	IMP: Inferred from Mutant Phenotype Assigned on 2012-10-21	manually curated	Berger AC, et al.  (2007) The subcellular localization of the Niemann-Pick Type C proteins depends on the adaptor complex AP-3. J Cell Sci 120(Pt 20):3640-52	SGD
ARL1/YBR164C	IMP: Inferred from Mutant Phenotype Assigned on 2003-01-23	manually curated	Rosenwald AG, et al.  (2002) ARL1 and membrane traffic in Saccharomyces cerevisiae. Yeast 19(12):1039-56	SGD
BOR1/YNL275W	IMP: Inferred from Mutant Phenotype Assigned on 2007-03-08	manually curated	Decker BL and Wickner WT  (2006) Enolase activates homotypic vacuole fusion and protein transport to the vacuole in yeast. J Biol Chem 281(20):14523-8	SGD
BSD2/YBR290W	IMP: Inferred from Mutant Phenotype Assigned on 2002-09-18	manually curated	Liu XF and Culotta VC  (1999) Post-translation control of Nramp metal transport in yeast. Role of metal ions and the BSD2 gene. J Biol Chem 274(8):4863-8	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-07-15	manually curated	Hettema EH, et al.  (2004) Bsd2 binds the ubiquitin ligase Rsp5 and mediates the ubiquitination of transmembrane proteins. EMBO J 23(6):1279-88	SGD
CCZ1/YBR131W	IMP: Inferred from Mutant Phenotype Assigned on 2003-02-26	manually curated	Wang CW, et al.  (2002) The Ccz1-Mon1 protein complex is required for the late step of multiple vacuole delivery pathways. J Biol Chem 277(49):47917-27	SGD
DID2/YKR035W-A	IMP: Inferred from Mutant Phenotype Assigned on 2002-05-28	manually curated	Amerik AY, et al.  (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80	SGD
HSE1/YHL002W	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction with SGD:VPS27 Assigned on 2007-08-09	manually curated	Bilodeau PS, et al.  (2002) The Vps27p Hse1p complex binds ubiquitin and mediates endosomal protein sorting. Nat Cell Biol 4(7):534-9	SGD
LOA1/YPR139C	IMP: Inferred from Mutant Phenotype Assigned on 2002-10-16	high-throughput	Bonangelino CJ, et al.  (2002) Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae. Mol Biol Cell 13(7):2486-501	SGD
MON1/YGL124C	IMP: Inferred from Mutant Phenotype Assigned on 2002-10-01	high-throughput	Bonangelino CJ, et al.  (2002) Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae. Mol Biol Cell 13(7):2486-501	SGD
MON2/YNL297C	IMP: Inferred from Mutant Phenotype Assigned on 2002-10-01	high-throughput	Bonangelino CJ, et al.  (2002) Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae. Mol Biol Cell 13(7):2486-501	SGD
MVB12/YGR206W	IMP: Inferred from Mutant Phenotype Assigned on 2006-12-12	manually curated	Curtiss M, et al.  (2007) Efficient cargo sorting by ESCRT-I and the subsequent release of ESCRT-I from multivesicular bodies requires the subunit Mvb12. Mol Biol Cell 18(2):636-45	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-03-05	manually curated	Chu T, et al.  (2006) New component of ESCRT-I regulates endosomal sorting complex assembly. J Cell Biol 175(5):815-23	SGD
MVP1/YMR004W	IMP: Inferred from Mutant Phenotype Assigned on 2002-08-20	manually curated	Ekena K and Stevens TH  (1995) The Saccharomyces cerevisiae MVP1 gene interacts with VPS1 and is required for vacuolar protein sorting. Mol Cell Biol 15(3):1671-8	SGD
PEP1/YBL017C	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction Assigned on 2001-01-18	manually curated	Marcusson EG, et al.  (1994) The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene. Cell 77(4):579-86	SGD
ROY1/YMR258C	IGI: Inferred from Genetic Interaction with SGD:VPS21 Assigned on 2011-03-21	manually curated	Liu Y, et al.  (2011) Non-SCF-type F-box protein Roy1/Ymr258c interacts with a Rab5-like GTPase Ypt52 and inhibits Ypt52 function. Mol Biol Cell 22(9):1575-84	SGD
SNF8/YPL002C	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction Assigned on 2002-10-01	manually curated	Babst M, et al.  (2002) Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body. Dev Cell 3(2):283-9	SGD
SNX4/YJL036W	IMP: Inferred from Mutant Phenotype Assigned on 2006-09-11	manually curated	Hettema EH, et al.  (2003) Retromer and the sorting nexins Snx4/41/42 mediate distinct retrieval pathways from yeast endosomes. EMBO J 22(3):548-57	SGD
SRN2/YLR119W	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction Assigned on 2002-08-27	manually curated	Katzmann DJ, et al.  (2001) Ubiquitin-dependent sorting into the multivesicular body pathway requires the function of a conserved endosomal protein sorting complex, ESCRT-I. Cell 106(2):145-55	SGD
STP22/YCL008C	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction Assigned on 2002-08-27	manually curated	Katzmann DJ, et al.  (2001) Ubiquitin-dependent sorting into the multivesicular body pathway requires the function of a conserved endosomal protein sorting complex, ESCRT-I. Cell 106(2):145-55	SGD
VID24/YBR105C	IMP: Inferred from Mutant Phenotype Assigned on 2007-11-29	manually curated	Chiang MC and Chiang HL  (1998) Vid24p, a novel protein localized to the fructose-1, 6-bisphosphatase-containing vesicles, regulates targeting of fructose-1,6-bisphosphatase from the vesicles to the vacuole for degradation. J Cell Biol 140(6):1347-56	SGD
VMA3/YEL027W	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Umemoto N, et al.  (1990) Roles of the VMA3 gene product, subunit c of the vacuolar membrane H(+)-ATPase on vacuolar acidification and protein transport. A study with VMA3-disrupted mutants of Saccharomyces cerevisiae. J Biol Chem 265(30):18447-53	SGD
VPS1/YKR001C	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Ekena K, et al.  (1993) The VPS1 protein is a dynamin-like GTPase required for sorting proteins to the yeast vacuole. Ciba Found Symp 176():198-211; discussion 211-4	SGD
VPS13/YLL040C	IMP: Inferred from Mutant Phenotype Assigned on 2012-04-27	manually curated	Redding K, et al.  (1996) Allele-specific suppression of a defective trans-Golgi network (TGN) localization signal in Kex2p identifies three genes involved in localization of TGN transmembrane proteins. Mol Cell Biol 16(11):6208-17	SGD
VPS15/YBR097W	IMP: Inferred from Mutant Phenotype Assigned on 2006-07-19	manually curated	Stack JH, et al.  (1995) Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast. J Cell Biol 129(2):321-34	SGD
VPS16/YPL045W	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Rieder SE and Emr SD  (1997) A novel RING finger protein complex essential for a late step in protein transport to the yeast vacuole. Mol Biol Cell 8(11):2307-27	SGD
VPS21/YOR089C	IMP: Inferred from Mutant Phenotype Assigned on 2002-11-13	manually curated	Singer-Kruger B, et al.  (1994) Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast. J Cell Biol 125(2):283-98	SGD
VPS25/YJR102C	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction Assigned on 2002-10-01	manually curated	Babst M, et al.  (2002) Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body. Dev Cell 3(2):283-9	SGD
VPS27/YNR006W	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction with SGD:HSE1 Assigned on 2007-08-09	manually curated	Bilodeau PS, et al.  (2002) The Vps27p Hse1p complex binds ubiquitin and mediates endosomal protein sorting. Nat Cell Biol 4(7):534-9	SGD

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