GO term: protein N-linked glycosylation 
Ontology: Biological Process (GO:0006487)
Definition: A protein glycosylation process in which a carbohydrate or carbohydrate derivative unit is added to a protein via the N4 atom of peptidyl-asparagine, the omega-N of arginine, or the N1' atom peptidyl-tryptophan.
Synonyms: N-glycan biosynthesis; N-glycan metabolism; protein amino acid N-linked glycosylation
View Ontology:
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(text)
Definition: A protein glycosylation process in which a carbohydrate or carbohydrate derivative unit is added to a protein via the N4 atom of peptidyl-asparagine, the omega-N of arginine, or the N1' atom peptidyl-tryptophan.
Synonyms: N-glycan biosynthesis; N-glycan metabolism; protein amino acid N-linked glycosylation
View Ontology:
(graph) | (text)
This table lists the methods used to annotate genes either directly to the term
protein N-linked glycosylation (31 genes)
or to its variants containing one or more
qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.
Links to Additional Annotations:
| Annotation Method | GO Term | # Yeast Genes Annotated |
|---|---|---|
| Manually curated (download data) | protein N-linked glycosylation | 31 |
| High-throughput | none | none |
| Computational | none | none |
Links to Additional Annotations:
- View
annotations in multiple organisms using
- Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.
Annotation details for genes that have been directly annotated to the term
protein N-linked glycosylation or its variants containing one or more
qualifiers (NOT, contributes to, or colocalizes with).
| protein N-linked glycosylation 31 genes directly annotated to this term |
||||
|---|---|---|---|---|
| Locus | Evidence | Annotation Method | Reference | Assigned By |
| ALG1/YBR110W | IMP: Inferred from Mutant Phenotype Assigned on 2002-09-02 |
manually curated | Huffaker TC and Robbins PW (1983) Yeast mutants deficient in protein glycosylation. Proc Natl Acad Sci U S A 80(24):7466-70 |
SGD |
| ALG5/YPL227C | TAS: Traceable Author Statement Assigned on 2002-09-27 |
manually curated | Heesen S, et al. (1994) Isolation of the ALG5 locus encoding the UDP-glucose:dolichyl-phosphate glucosyltransferase from Saccharomyces cerevisiae. Eur J Biochem 224(1):71-9 |
SGD |
| ALG7/YBR243C | IMP: Inferred from Mutant Phenotype Assigned on 2007-04-18 |
manually curated | Mendelsohn RD, et al. (2005) A hypomorphic allele of the first N-glycosylation gene, ALG7, causes mitochondrial defects in yeast. Biochim Biophys Acta 1723(1-3):33-44 |
SGD |
| ALG8/YOR067C | IGI: Inferred from Genetic Interaction with SGD:WBP1 Assigned on 2007-04-30 |
manually curated | Stagljar I, et al. (1994) New phenotype of mutations deficient in glucosylation of the lipid-linked oligosaccharide: cloning of the ALG8 locus. Proc Natl Acad Sci U S A 91(13):5977-81 |
SGD |
| ANP1/YEL036C | IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2002-02-13 |
manually curated | Jungmann J and Munro S (1998) Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity. EMBO J 17(2):423-34 |
SGD |
| CAX4/YGR036C | IMP: Inferred from Mutant Phenotype Assigned on 2002-09-05 |
manually curated | van Berkel MA, et al. (1999) The Saccharomyces cerevisiae CWH8 gene is required for full levels of dolichol-linked oligosaccharides in the endoplasmic reticulum and for efficient N-glycosylation. Glycobiology 9(3):243-53 |
SGD |
| CWH41/YGL027C | IMP: Inferred from Mutant Phenotype Assigned on 2006-12-21 |
manually curated | Romero PA, et al. (1997) The yeast CWH41 gene encodes glucosidase I. Glycobiology 7(7):997-1004 |
SGD |
| DIE2/YGR227W | IMP: Inferred from Mutant Phenotype Assigned on 2002-07-08 |
manually curated | Burda P and Aebi M (1998) The ALG10 locus of Saccharomyces cerevisiae encodes the alpha-1,2 glucosyltransferase of the endoplasmic reticulum: the terminal glucose of the lipid-linked oligosaccharide is required for efficient N-linked glycosylation. Glycobiology 8(5):455-62 |
SGD |
| DPM1/YPR183W | IMP: Inferred from Mutant Phenotype Assigned on 2002-09-26 |
manually curated | Orlean P (1992) Enzymes that recognize dolichols participate in three glycosylation pathways and are required for protein secretion. Biochem Cell Biol 70(6):438-47 |
SGD |
| EOS1/YNL080C | IMP: Inferred from Mutant Phenotype Assigned on 2007-01-04 |
manually curated | Nakamura T, et al. (2007) EOS1, whose deletion confers sensitivity to oxidative stress, is involved in N-glycosylation in Saccharomyces cerevisiae. Biochem Biophys Res Commun 353(2):293-8 |
SGD |
| GNT1/YOR320C | IMP: Inferred from Mutant Phenotype Assigned on 2003-07-08 |
manually curated | Yoko-o T, et al. (2003) An N-acetylglucosaminyltransferase of the Golgi apparatus of the yeast Saccharomyces cerevisiae that can modify N-linked glycans. Glycobiology 13(8):581-9 |
SGD |
| GTB1/YDR221W | IMP: Inferred from Mutant Phenotype Assigned on 2006-01-19 |
manually curated | Wilkinson BM, et al. (2006) Yeast GTB1 encodes a subunit of glucosidase II required for glycoprotein processing in the endoplasmic reticulum. J Biol Chem 281(10):6325-33 |
SGD |
| KTR2/YKR061W | IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18 |
manually curated | Lussier M, et al. (1996) Functional characterization of the YUR1, KTR1, and KTR2 genes as members of the yeast KRE2/MNT1 mannosyltransferase gene family. J Biol Chem 271(18):11001-8 |
SGD |
| KTR4/YBR199W | ISS: Inferred from Sequence or structural Similarity Assigned on 2001-01-18 |
manually curated | Lussier M, et al. (1999) The KTR and MNN1 mannosyltransferase families of Saccharomyces cerevisiae. Biochim Biophys Acta 1426(2):323-34 |
SGD |
| KTR5/YNL029C | ISS: Inferred from Sequence or structural Similarity Assigned on 2001-01-18 |
manually curated | Lussier M, et al. (1999) The KTR and MNN1 mannosyltransferase families of Saccharomyces cerevisiae. Biochim Biophys Acta 1426(2):323-34 |
SGD |
| KTR6/YPL053C | IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18 |
manually curated | Wang XH, et al. (1997) MNN6, a member of the KRE2/MNT1 family, is the gene for mannosylphosphate transfer in Saccharomyces cerevisiae. J Biol Chem 272(29):18117-24 |
SGD |
| KTR7/YIL085C | ISS: Inferred from Sequence or structural Similarity Assigned on 2001-01-18 |
manually curated | Lussier M, et al. (1999) The KTR and MNN1 mannosyltransferase families of Saccharomyces cerevisiae. Biochim Biophys Acta 1426(2):323-34 |
SGD |
| MNN4/YKL201C | IMP: Inferred from Mutant Phenotype Assigned on 2002-11-13 |
manually curated | Odani T, et al. (1996) Cloning and analysis of the MNN4 gene required for phosphorylation of N-linked oligosaccharides in Saccharomyces cerevisiae. Glycobiology 6(8):805-10 |
SGD |
| MNN9/YPL050C | IDA: Inferred from Direct Assay Assigned on 2002-02-13 |
manually curated | Jungmann J and Munro S (1998) Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity. EMBO J 17(2):423-34 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2012-05-15 |
manually curated | Rodionov D, et al. (2009) Expression and purification of recombinant M-Pol I from Saccharomyces cerevisiae with alpha-1,6 mannosylpolymerase activity. Protein Expr Purif 66(1):1-6 |
SGD | |
| OCH1/YGL038C | IMP: Inferred from Mutant Phenotype Assigned on 2005-05-11 |
manually curated | Lehle L, et al. (1995) Glycoprotein biosynthesis in Saccharomyces cerevisiae: ngd29, an N-glycosylation mutant allelic to och1 having a defect in the initiation of outer chain formation. FEBS Lett 370(1-2):41-5 |
SGD |
| OST1/YJL002C | IDA: Inferred from Direct Assay Assigned on 2001-01-18 |
manually curated | Pathak R and Imperiali B (1997) A dual affinity tag on the 64-kDa Nlt1p subunit allows the rapid characterization of mutant yeast oligosaccharyl transferase complexes. Arch Biochem Biophys 338(1):1-6 |
SGD |
| OST2/YOR103C | IPI: Inferred from Physical Interaction Assigned on 2002-10-01 |
manually curated | Karaoglu D, et al. (1997) The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p. J Biol Chem 272(51):32513-20 |
SGD |
| IMP: Inferred from Mutant Phenotype Assigned on 2010-08-16 |
manually curated | Silberstein S, et al. (1995) The essential OST2 gene encodes the 16-kD subunit of the yeast oligosaccharyltransferase, a highly conserved protein expressed in diverse eukaryotic organisms. J Cell Biol 131(2):371-83 |
SGD | |
| OST3/YOR085W | IPI: Inferred from Physical Interaction Assigned on 2002-10-01 |
manually curated | Karaoglu D, et al. (1997) The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p. J Biol Chem 272(51):32513-20 |
SGD |
| OST4/YDL232W | IPI: Inferred from Physical Interaction Assigned on 2002-10-01 |
manually curated | Karaoglu D, et al. (1997) The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p. J Biol Chem 272(51):32513-20 |
SGD |
| IMP: Inferred from Mutant Phenotype Assigned on 2009-09-28 |
manually curated | Chi JH, et al. (1996) The OST4 gene of Saccharomyces cerevisiae encodes an unusually small protein required for normal levels of oligosaccharyltransferase activity. J Biol Chem 271(6):3132-40 |
UniProtKB | |
| TAS: Traceable Author Statement Assigned on 2002-10-01 |
manually curated | Reiss G, et al. (1997) A specific screen for oligosaccharyltransferase mutations identifies the 9 kDa OST5 protein required for optimal activity in vivo and in vitro. EMBO J 16(6):1164-72 |
SGD | |
| OST5/YGL226C-A | IPI: Inferred from Physical Interaction Assigned on 2002-10-01 |
manually curated | Karaoglu D, et al. (1997) The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p. J Biol Chem 272(51):32513-20 |
SGD |
| ROT1/YMR200W | IGI: Inferred from Genetic Interaction with SGD:OST3, IPI: Inferred from Physical Interaction with SGD:OST3 Assigned on 2012-04-24 |
manually curated | Pasikowska M, et al. (2012) The essential endoplasmic reticulum chaperone Rot1 is required for protein N- and O-glycosylation in yeast. Glycobiology 22(7):939-47 |
SGD |
| STT3/YGL022W | IPI: Inferred from Physical Interaction Assigned on 2002-10-01 |
manually curated | Karaoglu D, et al. (1997) The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p. J Biol Chem 272(51):32513-20 |
SGD |
| SWP1/YMR149W | IPI: Inferred from Physical Interaction Assigned on 2002-10-01 |
manually curated | Karaoglu D, et al. (1997) The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p. J Biol Chem 272(51):32513-20 |
SGD |
| VAN1/YML115C | IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2002-02-13 |
manually curated | Jungmann J and Munro S (1998) Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity. EMBO J 17(2):423-34 |
SGD |
| WBP1/YEL002C | IPI: Inferred from Physical Interaction Assigned on 2002-10-01 |
manually curated | Karaoglu D, et al. (1997) The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p. J Biol Chem 272(51):32513-20 |
SGD |
