GO term: protein phosphorylation 
Ontology: Biological Process (GO:0006468)
Definition: The process of introducing a phosphate group on to a protein.
Synonyms: protein amino acid phosphorylation
View Ontology:
(graph) | 
(text)
Definition: The process of introducing a phosphate group on to a protein.
Synonyms: protein amino acid phosphorylation
View Ontology:
(graph) | (text)
This table lists the methods used to annotate genes either directly to the term
protein phosphorylation (133 genes)
or to its variants containing one or more
qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.
Links to Additional Annotations:
| Annotation Method | GO Term | # Yeast Genes Annotated |
|---|---|---|
| Manually curated (download data) | protein phosphorylation | 99 |
| High-throughput (download data) | protein phosphorylation | 82 |
| Computational (download data) | protein phosphorylation | 118 |
Links to Additional Annotations:
- View
annotations in multiple organisms using
- Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.
Annotation details for genes that have been directly annotated to the term
protein phosphorylation or its variants containing one or more
qualifiers (NOT, contributes to, or colocalizes with).
| protein phosphorylation 133 genes directly annotated to this term |
||||
|---|---|---|---|---|
| Locus | Evidence | Annotation Method | Reference | Assigned By |
| AKL1/YBR059C | IDA: Inferred from Direct Assay Assigned on 2012-05-04 |
high-throughput | Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:IPR008271, EBI:IPR000719 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| ALK1/YGL021W | IDA: Inferred from Direct Assay Assigned on 2006-10-31 |
manually curated | Nespoli A, et al. (2006) Alk1 and Alk2 are two new cell cycle-regulated haspin-like proteins in budding yeast. Cell Cycle 5(13):1464-71 |
SGD |
| ALK2/YBL009W | IDA: Inferred from Direct Assay Assigned on 2006-10-31 |
manually curated | Nespoli A, et al. (2006) Alk1 and Alk2 are two new cell cycle-regulated haspin-like proteins in budding yeast. Cell Cycle 5(13):1464-71 |
SGD |
| ARK1/YNL020C | IMP: Inferred from Mutant Phenotype, ISS: Inferred from Sequence or structural Similarity Assigned on 2001-01-18 |
manually curated | Cope MJ, et al. (1999) Novel protein kinases Ark1p and Prk1p associate with and regulate the cortical actin cytoskeleton in budding yeast. J Cell Biol 144(6):1203-18 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2009-02-23 |
manually curated | Jin M and Cai M (2008) A Novel Function of Arp2p in Mediating Prk1p-specific Regulation of Actin and Endocytosis in Yeast. Mol Biol Cell 19(1):297-307 |
SGD | |
| IDA: Inferred from Direct Assay Assigned on 2012-05-04 |
high-throughput | Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:IPR008271, EBI:IPR000719 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| ATG1/YGL180W | IMP: Inferred from Mutant Phenotype Assigned on 2008-05-13 |
manually curated | Matsuura A, et al. (1997) Apg1p, a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae. Gene 192(2):245-50 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2012-05-04 |
high-throughput | Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR002290, EBI:IPR008271 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| BCK1/YJL095W | TAS: Traceable Author Statement Assigned on 2001-01-18 |
manually curated | Costigan C and Snyder M (1994) SLK1, a yeast homolog of MAP kinase activators, has a RAS/cAMP-independent role in nutrient sensing. Mol Gen Genet 243(3):286-96 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2012-05-04 |
high-throughput | Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR002290, EBI:IPR008271 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| BUB1/YGR188C | IDA: Inferred from Direct Assay Assigned on 2003-02-13 |
manually curated | Roberts BT, et al. (1994) The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase. Mol Cell Biol 14(12):8282-91 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR008271 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| BUD32/YGR262C | IDA: Inferred from Direct Assay Assigned on 2002-10-10 |
manually curated | Stocchetto S, et al. (1997) Biochemical evidence that Saccharomyces cerevisiae YGR262c gene, required for normal growth, encodes a novel Ser/Thr-specific protein kinase. FEBS Lett 414(1):171-5 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:IPR008266, EBI:IPR000719 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| CAK1/YFL029C | IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2005-11-02 |
manually curated | Kaldis P, et al. (1996) The Cdk-activating kinase (CAK) from budding yeast. Cell 86(4):553-64 |
SGD |
| IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2005-11-02 |
manually curated | Thuret JY, et al. (1996) Civ1 (CAK in vivo), a novel Cdk-activating kinase. Cell 86(4):565-76 |
SGD | |
| IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2005-11-02 |
manually curated | Espinoza FH, et al. (1996) A cyclin-dependent kinase-activating kinase (CAK) in budding yeast unrelated to vertebrate CAK. Science 273(5282):1714-7 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:IPR008271, EBI:IPR000719 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| CBK1/YNL161W | IDA: Inferred from Direct Assay Assigned on 2008-09-11 |
manually curated | Mazanka E, et al. (2008) The NDR/LATS family kinase Cbk1 directly controls transcriptional asymmetry. PLoS Biol 6(8):e203 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:IPR000961, EBI:IPR002290, EBI:IPR008271, EBI:IPR017892, EBI:IPR000719 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| CDC15/YAR019C | IDA: Inferred from Direct Assay Assigned on 2002-09-18 |
manually curated | Mah AS, et al. (2001) Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex. Proc Natl Acad Sci U S A 98(13):7325-30 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2012-05-04 |
high-throughput | Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR002290, EBI:IPR008271 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| CDC28/YBR160W | IDA: Inferred from Direct Assay Assigned on 2012-05-04 |
high-throughput | Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR002290, EBI:IPR008271 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| CDC5/YMR001C | IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2006-12-07 |
manually curated | Kitada K, et al. (1993) A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5. Mol Cell Biol 13(7):4445-57 |
SGD |
| IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2006-12-05 |
manually curated | Darieva Z, et al. (2006) Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. Nature 444(7118):494-8 |
SGD | |
| IDA: Inferred from Direct Assay Assigned on 2012-05-04 |
high-throughput | Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:IPR002290, EBI:IPR008271, EBI:IPR000719 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| CDC7/YDL017W | IMP: Inferred from Mutant Phenotype Assigned on 2006-12-15 |
manually curated | Hollingsworth RE Jr and Sclafani RA (1990) DNA metabolism gene CDC7 from yeast encodes a serine (threonine) protein kinase. Proc Natl Acad Sci U S A 87(16):6272-6 |
SGD |
| IMP: Inferred from Mutant Phenotype Assigned on 2006-12-15 |
manually curated | Buck V, et al. (1991) CDC7 protein kinase activity is required for mitosis and meiosis in Saccharomyces cerevisiae. Mol Gen Genet 227(3):452-7 |
SGD | |
| IMP: Inferred from Mutant Phenotype Assigned on 2006-12-15 |
manually curated | Yoon HJ and Campbell JL (1991) The CDC7 protein of Saccharomyces cerevisiae is a phosphoprotein that contains protein kinase activity. Proc Natl Acad Sci U S A 88(9):3574-8 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR008271 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| CHK1/YBR274W | IDA: Inferred from Direct Assay Assigned on 2007-08-21 |
manually curated | Wang H, et al. (2001) Pds1 phosphorylation in response to DNA damage is essential for its DNA damage checkpoint function. Genes Dev 15(11):1361-72 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR002290, EBI:IPR008271 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| CKA1/YIL035C | IMP: Inferred from Mutant Phenotype Assigned on 2010-06-30 |
manually curated | Berkey CD and Carlson M (2006) A specific catalytic subunit isoform of protein kinase CK2 is required for phosphorylation of the repressor Nrg1 in Saccharomyces cerevisiae. Curr Genet 50(1):1-10 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2010-06-30 |
manually curated | Barz T, et al. (2006) Control of methionine biosynthesis genes by protein kinase CK2-mediated phosphorylation of Cdc34. Cell Mol Life Sci 63(18):2183-90 |
SGD | |
| IDA: Inferred from Direct Assay Assigned on 2012-05-04 |
high-throughput | Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:IPR008271, EBI:IPR000719 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| CKA2/YOR061W | IDA: Inferred from Direct Assay Assigned on 2010-06-30 |
manually curated | Barz T, et al. (2006) Control of methionine biosynthesis genes by protein kinase CK2-mediated phosphorylation of Cdc34. Cell Mol Life Sci 63(18):2183-90 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:IPR008271, EBI:IPR000719 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| CKB1/YGL019W | IMP: Inferred from Mutant Phenotype Assigned on 2010-06-30 |
manually curated | Berkey CD and Carlson M (2006) A specific catalytic subunit isoform of protein kinase CK2 is required for phosphorylation of the repressor Nrg1 in Saccharomyces cerevisiae. Curr Genet 50(1):1-10 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2010-06-30 |
manually curated | Barz T, et al. (2006) Control of methionine biosynthesis genes by protein kinase CK2-mediated phosphorylation of Cdc34. Cell Mol Life Sci 63(18):2183-90 |
SGD | |
| CKB2/YOR039W | IMP: Inferred from Mutant Phenotype Assigned on 2010-06-30 |
manually curated | Berkey CD and Carlson M (2006) A specific catalytic subunit isoform of protein kinase CK2 is required for phosphorylation of the repressor Nrg1 in Saccharomyces cerevisiae. Curr Genet 50(1):1-10 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2010-06-30 |
manually curated | Barz T, et al. (2006) Control of methionine biosynthesis genes by protein kinase CK2-mediated phosphorylation of Cdc34. Cell Mol Life Sci 63(18):2183-90 |
SGD | |
| CLA4/YNL298W | IDA: Inferred from Direct Assay Assigned on 2012-05-04 |
high-throughput | Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR002290, EBI:IPR008271 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| CMK1/YFR014C | IDA: Inferred from Direct Assay Assigned on 2001-01-18 |
manually curated | Ohya Y, et al. (1991) Two yeast genes encoding calmodulin-dependent protein kinases. Isolation, sequencing and bacterial expressions of CMK1 and CMK2. J Biol Chem 266(19):12784-94 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2012-05-04 |
high-throughput | Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR002290, EBI:IPR008271 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| CMK2/YOL016C | IDA: Inferred from Direct Assay Assigned on 2001-01-18 |
manually curated | Ohya Y, et al. (1991) Two yeast genes encoding calmodulin-dependent protein kinases. Isolation, sequencing and bacterial expressions of CMK1 and CMK2. J Biol Chem 266(19):12784-94 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2012-05-04 |
high-throughput | Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR002290, EBI:IPR008271 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| CTK1/YKL139W | IMP: Inferred from Mutant Phenotype, ISS: Inferred from Sequence or structural Similarity with SGD:CDC28 Assigned on 2007-01-22 |
manually curated | Lee JM and Greenleaf AL (1991) CTD kinase large subunit is encoded by CTK1, a gene required for normal growth of Saccharomyces cerevisiae. Gene Expr 1(2):149-67 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2012-05-04 |
high-throughput | Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR002290, EBI:IPR008271 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| CTK2/YJL006C | IPI: Inferred from Physical Interaction with SGD:CTK1 Assigned on 2007-01-22 |
manually curated | Sterner DE, et al. (1995) The yeast carboxyl-terminal repeat domain kinase CTDK-I is a divergent cyclin-cyclin-dependent kinase complex. Mol Cell Biol 15(10):5716-24 |
SGD |
| CTK3/YML112W | IPI: Inferred from Physical Interaction with SGD:CTK1 Assigned on 2006-09-25 |
manually curated | Sterner DE, et al. (1995) The yeast carboxyl-terminal repeat domain kinase CTDK-I is a divergent cyclin-cyclin-dependent kinase complex. Mol Cell Biol 15(10):5716-24 |
SGD |
| DBF2/YGR092W | IDA: Inferred from Direct Assay Assigned on 2003-02-04 |
manually curated | Mah AS, et al. (2001) Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex. Proc Natl Acad Sci U S A 98(13):7325-30 |
SGD |
| IMP: Inferred from Mutant Phenotype Assigned on 2008-09-26 |
manually curated | Makrantoni V, et al. (2007) A novel role for the yeast protein kinase Dbf2p in vacuolar H+-ATPase function and sorbic acid stress tolerance. Microbiology 153(Pt 12):4016-26 |
SGD | |
| IDA: Inferred from Direct Assay Assigned on 2012-05-04 |
high-throughput | Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:IPR000961, EBI:IPR002290, EBI:IPR008271, EBI:IPR017892, EBI:IPR000719 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| DBF20/YPR111W | IDA: Inferred from Direct Assay Assigned on 2002-11-18 |
manually curated | Toyn JH and Johnston LH (1994) The Dbf2 and Dbf20 protein kinases of budding yeast are activated after the metaphase to anaphase cell cycle transition. EMBO J 13(5):1103-13 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:IPR000961, EBI:IPR002290, EBI:IPR008271, EBI:IPR017892, EBI:IPR000719 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| DUN1/YDL101C | IDA: Inferred from Direct Assay Assigned on 2002-11-12 |
manually curated | Zhao X and Rothstein R (2002) The Dun1 checkpoint kinase phosphorylates and regulates the ribonucleotide reductase inhibitor Sml1. Proc Natl Acad Sci U S A 99(6):3746-51 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2012-05-04 |
high-throughput | Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:IPR000719, EBI:IPR002290, EBI:IPR008271 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| ELM1/YKL048C | IDA: Inferred from Direct Assay Assigned on 2007-08-14 |
manually curated | Koehler CM and Myers AM (1997) Serine-threonine protein kinase activity of Elm1p, a regulator of morphologic differentiation in Saccharomyces cerevisiae. FEBS Lett 408(1):109-14 |
SGD |
| IDA: Inferred from Direct Assay, IGI: Inferred from Genetic Interaction with SGD:SAK1, SGD:TOS3 Assigned on 2007-08-14 |
manually curated | Hong SP, et al. (2003) Activation of yeast Snf1 and mammalian AMP-activated protein kinase by upstream kinases. Proc Natl Acad Sci U S A 100(15):8839-43 |
SGD | |
| IDA: Inferred from Direct Assay Assigned on 2007-08-14 |
manually curated | Elbing K, et al. (2006) Purification and characterization of the three Snf1-activating kinases of Saccharomyces cerevisiae. Biochem J 393(Pt 3):797-805 |
SGD | |
| IDA: Inferred from Direct Assay Assigned on 2012-05-04 |
high-throughput | Ptacek J, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:IPR008271, EBI:IPR000719 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro | |
| ENV7/YPL236C | IEA: Inferred from Electronic Annotation with EBI:IPR000719 Last Updated 2013-03-02 |
computational | DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms. |
InterPro |
