Ontology: Biological Process (GO:0006303)

Definition: The repair of a double-strand break in DNA in which the two broken ends are rejoined with little or no sequence complementarity. Information at the DNA ends may be lost due to the modification of broken DNA ends.

Synonyms: NHEJ

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term double-strand break repair via nonhomologous end joining (26 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	double-strand break repair via nonhomologous end joining	26
High-throughput	none	none
Computational (download data)	double-strand break repair via nonhomologous end joining	2

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term double-strand break repair via nonhomologous end joining or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

double-strand break repair via nonhomologous end joining      26 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
DNL4/YOR005C	IDA: Inferred from Direct Assay Assigned on 2004-11-22	manually curated	Tseng HM and Tomkinson AE  (2004) Processing and joining of DNA ends coordinated by interactions among Dnl4/Lif1, Pol4, and FEN-1. J Biol Chem 279(46):47580-8	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Wilson TE, et al.  (1997) Yeast DNA ligase IV mediates non-homologous DNA end joining. Nature 388(6641):495-8	SGD
DOA1/YKL213C	IMP: Inferred from Mutant Phenotype Assigned on 2003-05-28	manually curated	Wilson TE  (2002) A genomics-based screen for yeast mutants with an altered recombination/end-joining repair ratio. Genetics 162(2):677-88	SGD
FYV6/YNL133C	IMP: Inferred from Mutant Phenotype Assigned on 2003-05-28	manually curated	Wilson TE  (2002) A genomics-based screen for yeast mutants with an altered recombination/end-joining repair ratio. Genetics 162(2):677-88	SGD
LIF1/YGL090W	IPI: Inferred from Physical Interaction Assigned on 2001-01-18	manually curated	Herrmann G, et al.  (1998) Saccharomyces cerevisiae LIF1: a function involved in DNA double-strand break repair related to mammalian XRCC4. EMBO J 17(14):4188-98	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-11-22	manually curated	Tseng HM and Tomkinson AE  (2004) Processing and joining of DNA ends coordinated by interactions among Dnl4/Lif1, Pol4, and FEN-1. J Biol Chem 279(46):47580-8	SGD
MCK1/YNL307C	IMP: Inferred from Mutant Phenotype Assigned on 2003-05-28	manually curated	Wilson TE  (2002) A genomics-based screen for yeast mutants with an altered recombination/end-joining repair ratio. Genetics 162(2):677-88	SGD
MRE11/YMR224C	IMP: Inferred from Mutant Phenotype Assigned on 2003-05-28	manually curated	Wilson TE  (2002) A genomics-based screen for yeast mutants with an altered recombination/end-joining repair ratio. Genetics 162(2):677-88	SGD
NEJ1/YLR265C	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-26	manually curated	Kegel A, et al.  (2001) Nej1p, a cell type-specific regulator of nonhomologous end joining in yeast. Curr Biol 11(20):1611-7	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-26	manually curated	Wu D, et al.  (2008) Recruitment and dissociation of nonhomologous end joining proteins at a DNA double-strand break in Saccharomyces cerevisiae. Genetics 178(3):1237-49	SGD
POL2/YNL262W	IGI: Inferred from Genetic Interaction with SGD:POL4, SGD:DNL4, IMP: Inferred from Mutant Phenotype Assigned on 2008-09-03	manually curated	Tseng SF, et al.  (2008) Proofreading activity of DNA polymerase Pol2 mediates 3'-end processing during nonhomologous end joining in yeast. PLoS Genet 4(4):e1000060	SGD
POL4/YCR014C	IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2008-09-03	manually curated	Wilson TE and Lieber MR  (1999) Efficient processing of DNA ends during yeast nonhomologous end joining. Evidence for a DNA polymerase beta (Pol4)-dependent pathway. J Biol Chem 274(33):23599-609	SGD
	IDA: Inferred from Direct Assay Assigned on 2004-11-22	manually curated	Tseng HM and Tomkinson AE  (2004) Processing and joining of DNA ends coordinated by interactions among Dnl4/Lif1, Pol4, and FEN-1. J Biol Chem 279(46):47580-8	SGD
RAD27/YKL113C	IDA: Inferred from Direct Assay Assigned on 2004-11-22	manually curated	Tseng HM and Tomkinson AE  (2004) Processing and joining of DNA ends coordinated by interactions among Dnl4/Lif1, Pol4, and FEN-1. J Biol Chem 279(46):47580-8	SGD
RAD50/YNL250W	IMP: Inferred from Mutant Phenotype Assigned on 2003-05-28	manually curated	Wilson TE  (2002) A genomics-based screen for yeast mutants with an altered recombination/end-joining repair ratio. Genetics 162(2):677-88	SGD
RSC1/YGR056W	IPI: Inferred from Physical Interaction with SGD:MRE11 Assigned on 2005-10-20	manually curated	Shim EY, et al.  (2005) The yeast chromatin remodeler RSC complex facilitates end joining repair of DNA double-strand breaks. Mol Cell Biol 25(10):3934-44	SGD
RSC2/YLR357W	IPI: Inferred from Physical Interaction with SGD:MRE11 Assigned on 2005-10-20	manually curated	Shim EY, et al.  (2005) The yeast chromatin remodeler RSC complex facilitates end joining repair of DNA double-strand breaks. Mol Cell Biol 25(10):3934-44	SGD
RSC30/YHR056C	IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2005-10-20	manually curated	Shim EY, et al.  (2005) The yeast chromatin remodeler RSC complex facilitates end joining repair of DNA double-strand breaks. Mol Cell Biol 25(10):3934-44	SGD
RSC8/YFR037C	IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2005-10-20	manually curated	Shim EY, et al.  (2005) The yeast chromatin remodeler RSC complex facilitates end joining repair of DNA double-strand breaks. Mol Cell Biol 25(10):3934-44	SGD
RTT109/YLL002W	IMP: Inferred from Mutant Phenotype Assigned on 2008-06-26	manually curated	Jessulat M, et al.  (2008) Interacting proteins Rtt109 and Vps75 affect the efficiency of non-homologous end-joining in Saccharomyces cerevisiae. Arch Biochem Biophys 469(2):157-64	SGD
SIN3/YOL004W	IMP: Inferred from Mutant Phenotype Assigned on 2005-02-24	manually curated	Jazayeri A, et al.  (2004) Saccharomyces cerevisiae Sin3p facilitates DNA double-strand break repair. Proc Natl Acad Sci U S A 101(6):1644-9	SGD
SIR2/YDL042C	IDA: Inferred from Direct Assay Assigned on 2002-11-01	manually curated	Hegde V and Klein H  (2000) Requirement for the SRS2 DNA helicase gene in non-homologous end joining in yeast. Nucleic Acids Res 28(14):2779-83	SGD
SIR3/YLR442C	IDA: Inferred from Direct Assay Assigned on 2002-11-01	manually curated	Hegde V and Klein H  (2000) Requirement for the SRS2 DNA helicase gene in non-homologous end joining in yeast. Nucleic Acids Res 28(14):2779-83	SGD
SIR4/YDR227W	IDA: Inferred from Direct Assay Assigned on 2002-11-01	manually curated	Hegde V and Klein H  (2000) Requirement for the SRS2 DNA helicase gene in non-homologous end joining in yeast. Nucleic Acids Res 28(14):2779-83	SGD
SRS2/YJL092W	IDA: Inferred from Direct Assay Assigned on 2002-11-01	manually curated	Hegde V and Klein H  (2000) Requirement for the SRS2 DNA helicase gene in non-homologous end joining in yeast. Nucleic Acids Res 28(14):2779-83	SGD
SUB1/YMR039C	IMP: Inferred from Mutant Phenotype Assigned on 2013-04-27	manually curated	Yu L and Volkert MR  (2013) Differential Requirement for SUB1 in Chromosomal and Plasmid Double-Strand DNA Break Repair. PLoS One 8(3):e58015	SGD
VPS75/YNL246W	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction with SGD:RTT109 Assigned on 2008-06-26	manually curated	Jessulat M, et al.  (2008) Interacting proteins Rtt109 and Vps75 affect the efficiency of non-homologous end-joining in Saccharomyces cerevisiae. Arch Biochem Biophys 469(2):157-64	SGD
XRS2/YDR369C	IMP: Inferred from Mutant Phenotype Assigned on 2003-05-28	manually curated	Wilson TE  (2002) A genomics-based screen for yeast mutants with an altered recombination/end-joining repair ratio. Genetics 162(2):677-88	SGD
YKU70/YMR284W	IDA: Inferred from Direct Assay Assigned on 2002-11-01	manually curated	Hegde V and Klein H  (2000) Requirement for the SRS2 DNA helicase gene in non-homologous end joining in yeast. Nucleic Acids Res 28(14):2779-83	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR006164, EBI:IPR006165, EBI:IPR005160 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
YKU80/YMR106C	IDA: Inferred from Direct Assay Assigned on 2002-11-01	manually curated	Hegde V and Klein H  (2000) Requirement for the SRS2 DNA helicase gene in non-homologous end joining in yeast. Nucleic Acids Res 28(14):2779-83	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR006164, EBI:IPR024193, EBI:IPR005160 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

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