Ontology: Biological Process (GO:0006268)

Definition: The process in which interchain hydrogen bonds between two strands of DNA are broken or 'melted', generating unpaired template strands for DNA replication.

Synonyms: DNA unwinding factor; DNA unwinding during replication

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term DNA unwinding involved in replication (9 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	DNA unwinding involved in replication	9
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term DNA unwinding involved in replication or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

DNA unwinding involved in replication      9 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
HFM1/YGL251C	IDA: Inferred from Direct Assay Assigned on 2003-04-24	manually curated	Nakagawa T and Kolodner RD  (2002) The MER3 DNA helicase catalyzes the unwinding of holliday junctions. J Biol Chem 277(31):28019-24	SGD
MCM4/YPR019W	IDA: Inferred from Direct Assay Assigned on 2007-08-14	manually curated	Kaplan DL, et al.  (2003) Mcm4,6,7 uses a "pump in ring" mechanism to unwind DNA by steric exclusion and actively translocate along a duplex. J Biol Chem 278(49):49171-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-16	manually curated	Biswas-Fiss EE, et al.  (2005) The Mcm467 complex of Saccharomyces cerevisiae is preferentially activated by autonomously replicating DNA sequences. Biochemistry 44(8):2916-25	SGD
MCM6/YGL201C	IDA: Inferred from Direct Assay Assigned on 2007-08-14	manually curated	Kaplan DL, et al.  (2003) Mcm4,6,7 uses a "pump in ring" mechanism to unwind DNA by steric exclusion and actively translocate along a duplex. J Biol Chem 278(49):49171-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-16	manually curated	Biswas-Fiss EE, et al.  (2005) The Mcm467 complex of Saccharomyces cerevisiae is preferentially activated by autonomously replicating DNA sequences. Biochemistry 44(8):2916-25	SGD
MCM7/YBR202W	IDA: Inferred from Direct Assay Assigned on 2007-08-14	manually curated	Kaplan DL, et al.  (2003) Mcm4,6,7 uses a "pump in ring" mechanism to unwind DNA by steric exclusion and actively translocate along a duplex. J Biol Chem 278(49):49171-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-16	manually curated	Biswas-Fiss EE, et al.  (2005) The Mcm467 complex of Saccharomyces cerevisiae is preferentially activated by autonomously replicating DNA sequences. Biochemistry 44(8):2916-25	SGD
RFA1/YAR007C	IDA: Inferred from Direct Assay Assigned on 2007-09-18	manually curated	Brill SJ and Stillman B  (1989) Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication. Nature 342(6245):92-5	SGD
RFA2/YNL312W	IDA: Inferred from Direct Assay Assigned on 2007-09-18	manually curated	Brill SJ and Stillman B  (1989) Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication. Nature 342(6245):92-5	SGD
RFA3/YJL173C	IDA: Inferred from Direct Assay Assigned on 2007-09-18	manually curated	Brill SJ and Stillman B  (1989) Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication. Nature 342(6245):92-5	SGD
SGS1/YMR190C	IDA: Inferred from Direct Assay Assigned on 2002-08-06	manually curated	Bennett RJ, et al.  (1999) Binding specificity determines polarity of DNA unwinding by the Sgs1 protein of S. cerevisiae. J Mol Biol 289(2):235-48	SGD
SLD3/YGL113W	IMP: Inferred from Mutant Phenotype Assigned on 2001-04-12	manually curated	Kamimura Y, et al.  (2001) Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae. EMBO J 20(8):2097-107	SGD

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