GO term: mRNA cleavage and polyadenylation specificity factor complex 
Ontology: Cellular Component (GO:0005847)
Definition: A multisubunit complex that binds to the canonical AAUAAA hexamer and to U-rich upstream sequence elements on the pre-mRNA, thereby stimulating the otherwise weakly active and nonspecific polymerase to elongate efficiently RNAs containing a poly(A) signal.
Synonyms: CFII complex; cleavage and polyadenylylation specificity factor activity; CPF complex; CPSF complex
View Ontology:
(graph) | 
(text)
Definition: A multisubunit complex that binds to the canonical AAUAAA hexamer and to U-rich upstream sequence elements on the pre-mRNA, thereby stimulating the otherwise weakly active and nonspecific polymerase to elongate efficiently RNAs containing a poly(A) signal.
Synonyms: CFII complex; cleavage and polyadenylylation specificity factor activity; CPF complex; CPSF complex
View Ontology:
(graph) | (text)
This table lists the methods used to annotate genes either directly to the term
mRNA cleavage and polyadenylation specificity factor complex (15 genes)
or to its variants containing one or more
qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.
Links to Additional Annotations:
| Annotation Method | GO Term | # Yeast Genes Annotated |
|---|---|---|
| Manually curated (download data) | mRNA cleavage and polyadenylation specificity factor complex | 15 |
| High-throughput | none | none |
| Computational | none | none |
Links to Additional Annotations:
- View
annotations in multiple organisms using
- Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.
Annotation details for genes that have been directly annotated to the term
mRNA cleavage and polyadenylation specificity factor complex or its variants containing one or more
qualifiers (NOT, contributes to, or colocalizes with).
| mRNA cleavage and polyadenylation specificity factor complex 15 genes directly annotated to this term |
||||
|---|---|---|---|---|
| Locus | Evidence | Annotation Method | Reference | Assigned By |
| CFT1/YDR301W | IPI: Inferred from Physical Interaction Assigned on 2004-09-28 |
manually curated | Zhao J, et al. (1997) Cleavage factor II of Saccharomyces cerevisiae contains homologues to subunits of the mammalian Cleavage/ polyadenylation specificity factor and exhibits sequence-specific, ATP-dependent interaction with precursor RNA. J Biol Chem 272(16):10831-8 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2012-04-05 |
manually curated | Nedea E, et al. (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10 |
SGD | |
| IPI: Inferred from Physical Interaction Assigned on 2004-09-28 |
manually curated | Zhao J, et al. (1999) Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor. Mol Cell Biol 19(11):7733-40 |
SGD | |
| CFT2/YLR115W | IPI: Inferred from Physical Interaction Assigned on 2004-09-28 |
manually curated | Zhao J, et al. (1997) Cleavage factor II of Saccharomyces cerevisiae contains homologues to subunits of the mammalian Cleavage/ polyadenylation specificity factor and exhibits sequence-specific, ATP-dependent interaction with precursor RNA. J Biol Chem 272(16):10831-8 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2012-04-05 |
manually curated | Nedea E, et al. (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10 |
SGD | |
| IPI: Inferred from Physical Interaction Assigned on 2004-09-28 |
manually curated | Zhao J, et al. (1999) Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor. Mol Cell Biol 19(11):7733-40 |
SGD | |
| FIP1/YJR093C | IDA: Inferred from Direct Assay Assigned on 2012-04-05 |
manually curated | Nedea E, et al. (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10 |
SGD |
| GLC7/YER133W | IDA: Inferred from Direct Assay Assigned on 2012-04-05 |
manually curated | Nedea E, et al. (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10 |
SGD |
| MPE1/YKL059C | IPI: Inferred from Physical Interaction Assigned on 2002-12-02 |
manually curated | Vo LT, et al. (2001) Mpe1, a zinc knuckle protein, is an essential component of yeast cleavage and polyadenylation factor required for the cleavage and polyadenylation of mRNA. Mol Cell Biol 21(24):8346-56 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2012-04-05 |
manually curated | Nedea E, et al. (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10 |
SGD | |
| PAP1/YKR002W | IDA: Inferred from Direct Assay Assigned on 2012-04-05 |
manually curated | Nedea E, et al. (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10 |
SGD |
| PFS2/YNL317W | IDA: Inferred from Direct Assay Assigned on 2012-04-05 |
manually curated | Nedea E, et al. (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10 |
SGD |
| PTA1/YAL043C | IDA: Inferred from Direct Assay Assigned on 2012-04-05 |
manually curated | Nedea E, et al. (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10 |
SGD |
| IPI: Inferred from Physical Interaction Assigned on 2004-09-28 |
manually curated | Zhao J, et al. (1999) Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor. Mol Cell Biol 19(11):7733-40 |
SGD | |
| PTI1/YGR156W | IDA: Inferred from Direct Assay Assigned on 2012-04-05 |
manually curated | Nedea E, et al. (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10 |
SGD |
| REF2/YDR195W | IDA: Inferred from Direct Assay Assigned on 2012-04-05 |
manually curated | Nedea E, et al. (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10 |
SGD |
| SSU72/YNL222W | IPI: Inferred from Physical Interaction with SGD:FIP1, SGD:PAP1, SGD:PFS2, SGD:YSH1 Assigned on 2012-06-20 |
manually curated | Dichtl B, et al. (2002) A role for SSU72 in balancing RNA polymerase II transcription elongation and termination. Mol Cell 10(5):1139-50 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2012-04-05 |
manually curated | Nedea E, et al. (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10 |
SGD | |
| SWD2/YKL018W | IDA: Inferred from Direct Assay Assigned on 2012-04-05 |
manually curated | Nedea E, et al. (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10 |
SGD |
| SYC1/YOR179C | IDA: Inferred from Direct Assay Assigned on 2012-04-05 |
manually curated | Nedea E, et al. (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10 |
SGD |
| YSH1/YLR277C | IPI: Inferred from Physical Interaction Assigned on 2004-09-28 |
manually curated | Zhao J, et al. (1997) Cleavage factor II of Saccharomyces cerevisiae contains homologues to subunits of the mammalian Cleavage/ polyadenylation specificity factor and exhibits sequence-specific, ATP-dependent interaction with precursor RNA. J Biol Chem 272(16):10831-8 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2012-04-05 |
manually curated | Nedea E, et al. (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10 |
SGD | |
| IPI: Inferred from Physical Interaction Assigned on 2004-09-28 |
manually curated | Zhao J, et al. (1999) Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor. Mol Cell Biol 19(11):7733-40 |
SGD | |
| YTH1/YPR107C | IDA: Inferred from Direct Assay Assigned on 2012-04-05 |
manually curated | Nedea E, et al. (2003) Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem 278(35):33000-10 |
SGD |
