Ontology: Cellular Component (GO:0005816)

Definition: The microtubule organizing center in fungi; functionally homologous to the animal cell centrosome.

Synonyms: SPB

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term spindle pole body (66 genes) or to its variants containing one or more qualifiers (14 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	colocalizes_with: spindle pole body	9
	spindle pole body	49
High-throughput (download data)	colocalizes_with: spindle pole body	5
	spindle pole body	3
Computational (download data)	spindle pole body	35

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term spindle pole body or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

spindle pole body      66 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
ADY3/YDL239C	IEA: Inferred from Electronic Annotation with EBI:SL-0252 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
ADY4/YLR227C	IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2003-07-08	manually curated	Nickas ME, et al.  (2003) Ady4p and Spo74p are components of the meiotic spindle pole body that promote growth of the prospore membrane in Saccharomyces cerevisiae. Eukaryot Cell 2(3):431-45	SGD
	IEA: Inferred from Electronic Annotation with EBI:SL-0252 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
AME1/YBR211C	NAS: Non-traceable Author Statement Assigned on 2003-01-22	manually curated	Ross-Macdonald P, et al.  (1997) A multipurpose transposon system for analyzing protein production, localization, and function in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 94(1):190-5	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-12-12	high-throughput	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD
BBP1/YPL255W	IDA: Inferred from Direct Assay Assigned on 2001-01-18	manually curated	Wigge PA, et al.  (1998) Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J Cell Biol 141(4):967-77	SGD
	IEA: Inferred from Electronic Annotation with EBI:SL-0252 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
BFA1/YJR053W	IDA: Inferred from Direct Assay Assigned on 2001-01-18	manually curated	Li R  (1999) Bifurcation of the mitotic checkpoint pathway in budding yeast. Proc Natl Acad Sci U S A 96(9):4989-94	SGD
BIK1/YCL029C	IDA: Inferred from Direct Assay Assigned on 2007-01-19	manually curated	Berlin V, et al.  (1990) BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin. J Cell Biol 111(6 Pt 1):2573-86	SGD
	IEA: Inferred from Electronic Annotation with EBI:SL-0252 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
BIM1/YER016W	IDA: Inferred from Direct Assay Assigned on 2001-01-18	manually curated	Wigge PA, et al.  (1998) Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J Cell Biol 141(4):967-77	SGD
BUB2/YMR055C	IDA: Inferred from Direct Assay Assigned on 2001-01-18	manually curated	Fraschini R, et al.  (1999) Budding yeast Bub2 is localized at spindle pole bodies and activates the mitotic checkpoint via a different pathway from Mad2. J Cell Biol 145(5):979-91	SGD
	IDA: Inferred from Direct Assay Assigned on 2001-01-18	manually curated	Li R  (1999) Bifurcation of the mitotic checkpoint pathway in budding yeast. Proc Natl Acad Sci U S A 96(9):4989-94	SGD
BUD6/YLR319C	IDA: Inferred from Direct Assay Assigned on 2007-07-11	manually curated	Haarer BK, et al.  (2007) Stable preanaphase spindle positioning requires Bud6p and an apparent interaction between the spindle pole bodies and the neck. Eukaryot Cell 6(5):797-807	SGD
CBF2/YGR140W	IEA: Inferred from Electronic Annotation with EBI:SL-0252 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
CDC14/YFR028C	IDA: Inferred from Direct Assay Assigned on 2002-09-18	manually curated	Yoshida S, et al.  (2002) Mitotic exit network controls the localization of Cdc14 to the spindle pole body in Saccharomyces cerevisiae. Curr Biol 12(11):944-50	SGD
CDC15/YAR019C	IDA: Inferred from Direct Assay Assigned on 2002-09-18	manually curated	Cenamor R, et al.  (1999) The budding yeast Cdc15 localizes to the spindle pole body in a cell-cycle-dependent manner. Mol Cell Biol Res Commun 2(3):178-84	SGD
CDC31/YOR257W	IEA: Inferred from Electronic Annotation with EBI:SL-0252 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
CIK1/YMR198W	IDA: Inferred from Direct Assay Assigned on 2002-02-08	manually curated	Page BD and Snyder M  (1992) CIK1: a developmentally regulated spindle pole body-associated protein important for microtubule functions in Saccharomyces cerevisiae. Genes Dev 6(8):1414-29	SGD
	IDA: Inferred from Direct Assay Assigned on 2002-02-08	manually curated	Shanks RM, et al.  (2001) The Kar3-interacting protein Cik1p plays a critical role in passage through meiosis I in Saccharomyces cerevisiae. Genetics 159(3):939-51	SGD
	IEA: Inferred from Electronic Annotation with EBI:SL-0252 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
CLB2/YPR119W	IDA: Inferred from Direct Assay Assigned on 2005-06-02	manually curated	Bailly E, et al.  (2003) Differential cellular localization among mitotic cyclins from Saccharomyces cerevisiae: a new role for the axial budding protein Bud3 in targeting Clb2 to the mother-bud neck. J Cell Sci 116(Pt 20):4119-30	SGD
CMD1/YBR109C	IEA: Inferred from Electronic Annotation with EBI:SL-0252 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
CNM67/YNL225C	IDA: Inferred from Direct Assay Assigned on 2001-01-18	manually curated	Wigge PA, et al.  (1998) Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J Cell Biol 141(4):967-77	SGD
	IEA: Inferred from Electronic Annotation with EBI:SL-0252 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
CRM1/YGR218W	IDA: Inferred from Direct Assay Assigned on 2008-08-08	manually curated	Neuber A, et al.  (2008) Nuclear export receptor Xpo1/Crm1 is physically and functionally linked to the spindle pole body in budding yeast. Mol Cell Biol 28(17):5348-58	SGD
DBF2/YGR092W	IDA: Inferred from Direct Assay Assigned on 2005-02-23	manually curated	Frenz LM, et al.  (2000) The budding yeast Dbf2 protein kinase localises to the centrosome and moves to the bud neck in late mitosis. J Cell Sci 113 Pt 19():3399-408	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-02-23	manually curated	Yoshida S and Toh-e A  (2001) Regulation of the localization of Dbf2 and mob1 during cell division of saccharomyces cerevisiae. Genes Genet Syst 76(2):141-7	SGD
DBF20/YPR111W	IDA: Inferred from Direct Assay Assigned on 2012-05-14	manually curated	Hwa Lim H, et al.  (2003) Inactivation of mitotic kinase triggers translocation of MEN components to mother-daughter neck in yeast. Mol Biol Cell 14(11):4734-43	SGD
DUO1/YGL061C	IDA: Inferred from Direct Assay Assigned on 2004-01-20	manually curated	Hofmann C, et al.  (1998) Saccharomyces cerevisiae Duo1p and Dam1p, novel proteins involved in mitotic spindle function. J Cell Biol 143(4):1029-40	SGD
DYN1/YKR054C	IDA: Inferred from Direct Assay Assigned on 2009-02-17	manually curated	Grava S, et al.  (2006) Asymmetric recruitment of dynein to spindle poles and microtubules promotes proper spindle orientation in yeast. Dev Cell 10(4):425-39	SGD
ESP1/YGR098C	IEA: Inferred from Electronic Annotation with EBI:SL-0252 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
FIN1/YDR130C	IDA: Inferred from Direct Assay Assigned on 2007-01-19	manually curated	van Hemert MJ, et al.  (2002) The Saccharomyces cerevisiae Fin1 protein forms cell cycle-specific filaments between spindle pole bodies. Proc Natl Acad Sci U S A 99(8):5390-3	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-01-19	manually curated	Woodbury EL and Morgan DO  (2007) Cdk and APC activities limit the spindle-stabilizing function of Fin1 to anaphase. Nat Cell Biol 9(1):106-12	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-06-08	manually curated	Woodbury EL and Morgan DO  (2007) The Role of Self-association in Fin1 Function on the Mitotic Spindle. J Biol Chem 282(44):32138-43	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-12-12	high-throughput	Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76	SGD
GLC7/YER133W	IDA: Inferred from Direct Assay Assigned on 2005-11-01	manually curated	Bloecher A and Tatchell K  (2000) Dynamic localization of protein phosphatase type 1 in the mitotic cell cycle of Saccharomyces cerevisiae. J Cell Biol 149(1):125-40	SGD
HRR25/YPL204W	IDA: Inferred from Direct Assay Assigned on 2009-02-26	manually curated	Lusk CP, et al.  (2007) Nup53p is a Target of Two Mitotic Kinases, Cdk1p and Hrr25p. Traffic 8(6):647-60	SGD
KAR1/YNL188W	IEA: Inferred from Electronic Annotation with EBI:SL-0252 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
KAR3/YPR141C	IDA: Inferred from Direct Assay Assigned on 2002-02-08	manually curated	Shanks RM, et al.  (2001) The Kar3-interacting protein Cik1p plays a critical role in passage through meiosis I in Saccharomyces cerevisiae. Genetics 159(3):939-51	SGD
	IDA: Inferred from Direct Assay Assigned on 2002-02-08	manually curated	Meluh PB and Rose MD  (1990) KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell 60(6):1029-41	SGD
	IDA: Inferred from Direct Assay Assigned on 2002-02-08	manually curated	Page BD, et al.  (1994) Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein. J Cell Biol 124(4):507-19	SGD
	IEA: Inferred from Electronic Annotation with EBI:SL-0252 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
KAR9/YPL269W	IDA: Inferred from Direct Assay Assigned on 2010-11-18	manually curated	Cepeda-Garcia C, et al.  (2010) Actin-mediated Delivery of Astral Microtubules Instructs Kar9p Asymmetric Loading to the Bud-Ward Spindle Pole. Mol Biol Cell 21(15):2685-95	SGD
KIN4/YOR233W	IDA: Inferred from Direct Assay Assigned on 2006-02-16	manually curated	Pereira G and Schiebel E  (2005) Kin4 kinase delays mitotic exit in response to spindle alignment defects. Mol Cell 19(2):209-21	SGD

colocalizes_with: spindle pole body      14 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
ADY3/YDL239C	IDA: Inferred from Direct Assay Assigned on 2011-01-04	manually curated	Moreno-Borchart AC, et al.  (2001) Prospore membrane formation linked to the leading edge protein (LEP) coat assembly. EMBO J 20(24):6946-57	SGD
	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:CNM67, SGD:NUD1, SGD:SPC42 Assigned on 2011-01-04	manually curated	Nickas ME and Neiman AM  (2002) Ady3p links spindle pole body function to spore wall synthesis in Saccharomyces cerevisiae. Genetics 160(4):1439-50	SGD
ARP1/YHR129C	IDA: Inferred from Direct Assay Assigned on 2009-05-21	manually curated	Woodruff JB, et al.  (2009) Dynein-driven mitotic spindle positioning restricted to anaphase by She1p inhibition of dynactin recruitment. Mol Biol Cell 20(13):3003-11	SGD
CDC28/YBR160W	IDA: Inferred from Direct Assay Assigned on 2008-01-31	manually curated	Maekawa H, et al.  (2003) Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions. EMBO J 22(3):438-49	SGD
CLB4/YLR210W	IDA: Inferred from Direct Assay Assigned on 2003-10-28	high-throughput	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
JNM1/YMR294W	IDA: Inferred from Direct Assay Assigned on 2009-05-21	manually curated	Woodruff JB, et al.  (2009) Dynein-driven mitotic spindle positioning restricted to anaphase by She1p inhibition of dynactin recruitment. Mol Biol Cell 20(13):3003-11	SGD
KRE28/YDR532C	IDA: Inferred from Direct Assay Assigned on 2003-10-28	high-throughput	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
LDB18/YLL049W	IDA: Inferred from Direct Assay Assigned on 2009-05-21	manually curated	Woodruff JB, et al.  (2009) Dynein-driven mitotic spindle positioning restricted to anaphase by She1p inhibition of dynactin recruitment. Mol Biol Cell 20(13):3003-11	SGD
MLP1/YKR095W	IDA: Inferred from Direct Assay Assigned on 2009-04-01	manually curated	Niepel M, et al.  (2005) The nuclear pore complex-associated protein, Mlp2p, binds to the yeast spindle pole body and promotes its efficient assembly. J Cell Biol 170(2):225-35	SGD
MLP2/YIL149C	IDA: Inferred from Direct Assay Assigned on 2009-04-01	manually curated	Niepel M, et al.  (2005) The nuclear pore complex-associated protein, Mlp2p, binds to the yeast spindle pole body and promotes its efficient assembly. J Cell Biol 170(2):225-35	SGD
NIP100/YPL174C	IDA: Inferred from Direct Assay Assigned on 2009-05-21	manually curated	Woodruff JB, et al.  (2009) Dynein-driven mitotic spindle positioning restricted to anaphase by She1p inhibition of dynactin recruitment. Mol Biol Cell 20(13):3003-11	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-05-12	manually curated	Kahana JA, et al.  (1998) The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B. Mol Biol Cell 9(7):1741-56	SGD
NKP2/YLR315W	IDA: Inferred from Direct Assay Assigned on 2003-10-28	high-throughput	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
NUF2/YOL069W	IDA: Inferred from Direct Assay Assigned on 2005-03-03	manually curated	Osborne MA, et al.  (1994) Nuf2, a spindle pole body-associated protein required for nuclear division in yeast. J Cell Biol 125(4):853-66	SGD
SGO1/YOR073W	IDA: Inferred from Direct Assay Assigned on 2003-10-28	high-throughput	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD
SLD7/YOR060C	IDA: Inferred from Direct Assay Assigned on 2003-10-28	high-throughput	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

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