GO term: nucleolus 
Ontology: Cellular Component (GO:0005730)
Definition: A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
View Ontology:
(graph) | 
(text)
Definition: A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
View Ontology:
(graph) | (text)
This table lists the methods used to annotate genes either directly to the term
nucleolus (337 genes)
or to its variants containing one or more
qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.
Links to Additional Annotations:
| Annotation Method | GO Term | # Yeast Genes Annotated |
|---|---|---|
| Manually curated (download data) | nucleolus | 252 |
| High-throughput (download data) | nucleolus | 66 |
| Computational (download data) | nucleolus | 207 |
Links to Additional Annotations:
- View
annotations in multiple organisms using
- Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.
Annotation details for genes that have been directly annotated to the term
nucleolus or its variants containing one or more
qualifiers (NOT, contributes to, or colocalizes with).
| nucleolus 337 genes directly annotated to this term |
||||
|---|---|---|---|---|
| Locus | Evidence | Annotation Method | Reference | Assigned By |
| ACS2/YLR153C | IDA: Inferred from Direct Assay Assigned on 2009-10-16 |
manually curated | Falcon AA, et al. (2010) Acetyl-coenzyme A synthetase 2 is a nuclear protein required for replicative longevity in Saccharomyces cerevisiae. Mol Cell Biochem 333(1-2):99-108 |
SGD |
| AIR1/YIL079C | IDA: Inferred from Direct Assay Assigned on 2003-05-12 |
manually curated | Drees BL, et al. (2001) A protein interaction map for cell polarity development. J Cell Biol 154(3):549-71 |
SGD |
| AIR2/YDL175C | IDA: Inferred from Direct Assay Assigned on 2009-08-24 |
manually curated | Dez C, et al. (2006) Surveillance of nuclear-restricted pre-ribosomes within a subnucleolar region of Saccharomyces cerevisiae. EMBO J 25(7):1534-46 |
SGD |
| BFR2/YDR299W | IDA: Inferred from Direct Assay Assigned on 2007-09-19 |
manually curated | Bernstein KA, et al. (2004) The small-subunit processome is a ribosome assembly intermediate. Eukaryot Cell 3(6):1619-26 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2003-10-28 |
high-throughput | Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| BMS1/YPL217C | IDA: Inferred from Direct Assay Assigned on 2001-01-18 |
manually curated | Rout MP, et al. (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| BMT2/YBR141C | IDA: Inferred from Direct Assay Assigned on 2013-04-23 |
manually curated | Sharma S, et al. (2013) Identification of a novel methyltransferase, Bmt2, responsible for the N-1-methyl-adenosine base modification of 25S rRNA in Saccharomyces cerevisiae. Nucleic Acids Res () |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2003-10-28 |
high-throughput | Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| BRX1/YOL077C | IPI: Inferred from Physical Interaction Assigned on 2002-08-26 |
manually curated | Fatica A, et al. (2002) Ssf1p prevents premature processing of an early pre-60S ribosomal particle. Mol Cell 9(2):341-51 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| BUD21/YOR078W | IDA: Inferred from Direct Assay Assigned on 2007-09-17 |
manually curated | Dragon F, et al. (2002) A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature 417(6892):967-70 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2008-04-07 |
manually curated | Grandi P, et al. (2002) 90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors. Mol Cell 10(1):105-15 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| BUD22/YMR014W | IDA: Inferred from Direct Assay Assigned on 2011-10-27 |
high-throughput | Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 |
SGD |
| BUD23/YCR047C | IDA: Inferred from Direct Assay Assigned on 2009-10-09 |
manually curated | White J, et al. (2008) Bud23 methylates G1575 of 18S rRNA and is required for efficient nuclear export of Pre-40S subunits. Mol Cell Biol 28(10):3151-61 |
SGD |
| CBF5/YLR175W | IC: Inferred By Curator from rRNA pseudouridine synthesis Assigned on 2006-09-25 |
manually curated | Lafontaine DLJ, et al. (1998) The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase. Genes Dev 12(4):527-37 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| CDC14/YFR028C | IDA: Inferred from Direct Assay Assigned on 2002-09-18 |
manually curated | Yoshida S, et al. (2002) Mitotic exit network controls the localization of Cdc14 to the spindle pole body in Saccharomyces cerevisiae. Curr Biol 12(11):944-50 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| CGR1/YGL029W | IDA: Inferred from Direct Assay Assigned on 2001-02-08 |
manually curated | Sun J, et al. (2001) Cgr1p, a novel nucleolar protein encoded by Saccharomyces cerevisiae orf YGL0292w. Curr Microbiol 42(1):65-9 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| CIC1/YHR052W | IDA: Inferred from Direct Assay Assigned on 2003-04-09 |
manually curated | Jager S, et al. (2001) Cic1, an adaptor protein specifically linking the 26S proteasome to its substrate, the SCF component Cdc4. EMBO J 20(16):4423-31 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| CSL4/YNL232W | IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB |
| CSM1/YCR086W | IDA: Inferred from Direct Assay Assigned on 2003-10-01 |
manually curated | Rabitsch KP, et al. (2003) Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I. Dev Cell 4(4):535-48 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| CTK1/YKL139W | IDA: Inferred from Direct Assay Assigned on 2008-10-30 |
manually curated | Bouchoux C, et al. (2004) CTD kinase I is involved in RNA polymerase I transcription. Nucleic Acids Res 32(19):5851-60 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| CTK2/YJL006C | IDA: Inferred from Direct Assay Assigned on 2008-10-30 |
manually curated | Bouchoux C, et al. (2004) CTD kinase I is involved in RNA polymerase I transcription. Nucleic Acids Res 32(19):5851-60 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| CTK3/YML112W | IDA: Inferred from Direct Assay Assigned on 2008-10-30 |
manually curated | Bouchoux C, et al. (2004) CTD kinase I is involved in RNA polymerase I transcription. Nucleic Acids Res 32(19):5851-60 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| DBP10/YDL031W | IDA: Inferred from Direct Assay Assigned on 2003-09-22 |
manually curated | Burger F, et al. (2000) Dbp10p, a putative RNA helicase from Saccharomyces cerevisiae, is required for ribosome biogenesis. Nucleic Acids Res 28(12):2315-23 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| DBP3/YGL078C | IDA: Inferred from Direct Assay Assigned on 2003-09-22 |
manually curated | Weaver PL, et al. (1997) Dbp3p, a putative RNA helicase in Saccharomyces cerevisiae, is required for efficient pre-rRNA processing predominantly at site A3. Mol Cell Biol 17(3):1354-65 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| DBP6/YNR038W | IDA: Inferred from Direct Assay Assigned on 2003-09-22 |
manually curated | Kressler D, et al. (1998) Dbp6p is an essential putative ATP-dependent RNA helicase required for 60S-ribosomal-subunit assembly in Saccharomyces cerevisiae. Mol Cell Biol 18(4):1855-65 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| DBP7/YKR024C | IDA: Inferred from Direct Assay Assigned on 2003-09-22 |
manually curated | Daugeron MC and Linder P (1998) Dbp7p, a putative ATP-dependent RNA helicase from Saccharomyces cerevisiae, is required for 60S ribosomal subunit assembly. RNA 4(5):566-81 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| DBP8/YHR169W | IDA: Inferred from Direct Assay Assigned on 2001-06-28 |
manually curated | Daugeron MC and Linder P (2001) Characterization and mutational analysis of yeast Dbp8p, a putative RNA helicase involved in ribosome biogenesis. Nucleic Acids Res 29(5):1144-55 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| DBP9/YLR276C | IDA: Inferred from Direct Assay Assigned on 2012-05-08 |
manually curated | Daugeron MC, et al. (2001) Dbp9p, a putative ATP-dependent RNA helicase involved in 60S-ribosomal-subunit biogenesis, functionally interacts with Dbp6p. RNA 7(9):1317-34 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| DHR2/YKL078W | IDA: Inferred from Direct Assay Assigned on 2002-03-19 |
manually curated | Colley A, et al. (2000) Dhr1p, a putative DEAH-box RNA helicase, is associated with the box C+D snoRNP U3. Mol Cell Biol 20(19):7238-46 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| DIM1/YPL266W | TAS: Traceable Author Statement Assigned on 2001-01-18 |
manually curated | Venema J and Tollervey D (1999) Ribosome synthesis in Saccharomyces cerevisiae. Annu Rev Genet 33:261-311 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| DIP2/YLR129W | IDA: Inferred from Direct Assay Assigned on 2007-09-17 |
manually curated | Dragon F, et al. (2002) A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature 417(6892):967-70 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| DIS3/YOL021C | IDA: Inferred from Direct Assay Assigned on 2009-01-29 |
manually curated | Shiomi T, et al. (1998) Human dis3p, which binds to either GTP- or GDP-Ran, complements Saccharomyces cerevisiae dis3. J Biochem (Tokyo) 123(5):883-90 |
SGD |
| IDA: Inferred from Direct Assay Assigned on 2012-12-12 |
high-throughput | Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76 |
SGD | |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
| DRS1/YLL008W | IDA: Inferred from Direct Assay Assigned on 2013-03-18 |
high-throughput | Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 |
SGD |
| IEA: Inferred from Electronic Annotation with EBI:SL-0188 Last Updated 2013-03-02 |
computational | UniProt-GOA (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries. |
UniProtKB | |
