Ontology: Cellular Component (GO:0005665)

Definition: RNA polymerase II, one of three nuclear DNA-directed RNA polymerases found in all eukaryotes, is a multisubunit complex; typically it produces mRNAs, snoRNAs, and some of the snRNAs. Two large subunits comprise the most conserved portion including the catalytic site and share similarity with other eukaryotic and bacterial multisubunit RNA polymerases. The largest subunit of RNA polymerase II contains an essential carboxyl-terminal domain (CTD) composed of a variable number of heptapeptide repeats (YSPTSPS). The remainder of the complex is composed of smaller subunits (generally ten or more), some of which are also found in RNA polymerases I and III. Although the core is competent to mediate ribonucleic acid synthesis, it requires additional factors to select the appropriate template.

Synonyms: RNA polymerase II complex; RNAP II complex

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term DNA-directed RNA polymerase II, core complex (12 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	DNA-directed RNA polymerase II, core complex	12
High-throughput	none	none
Computational (download data)	DNA-directed RNA polymerase II, core complex	1

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term DNA-directed RNA polymerase II, core complex or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

DNA-directed RNA polymerase II, core complex      12 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
RPB10/YOR210W	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Sayre MH, et al.  (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-19	manually curated	Carles C, et al.  (1991) Two additional common subunits, ABC10 alpha and ABC10 beta, are shared by yeast RNA polymerases. J Biol Chem 266(35):24092-6	SGD
RPB11/YOL005C	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Sayre MH, et al.  (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82	SGD
RPB2/YOR151C	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Kolodziej PA, et al.  (1990) RNA polymerase II subunit composition, stoichiometry, and phosphorylation. Mol Cell Biol 10(5):1915-20	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Sayre MH, et al.  (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Woychik NA, et al.  (1990) Subunits shared by eukaryotic nuclear RNA polymerases. Genes Dev 4(3):313-23	SGD
RPB3/YIL021W	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Kolodziej PA, et al.  (1990) RNA polymerase II subunit composition, stoichiometry, and phosphorylation. Mol Cell Biol 10(5):1915-20	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Sayre MH, et al.  (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Woychik NA, et al.  (1990) Subunits shared by eukaryotic nuclear RNA polymerases. Genes Dev 4(3):313-23	SGD
RPB4/YJL140W	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Kolodziej PA, et al.  (1990) RNA polymerase II subunit composition, stoichiometry, and phosphorylation. Mol Cell Biol 10(5):1915-20	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Sayre MH, et al.  (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Woychik NA, et al.  (1990) Subunits shared by eukaryotic nuclear RNA polymerases. Genes Dev 4(3):313-23	SGD
RPB5/YBR154C	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Kolodziej PA, et al.  (1990) RNA polymerase II subunit composition, stoichiometry, and phosphorylation. Mol Cell Biol 10(5):1915-20	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Sayre MH, et al.  (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Woychik NA, et al.  (1990) Subunits shared by eukaryotic nuclear RNA polymerases. Genes Dev 4(3):313-23	SGD
RPB7/YDR404C	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Kolodziej PA, et al.  (1990) RNA polymerase II subunit composition, stoichiometry, and phosphorylation. Mol Cell Biol 10(5):1915-20	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Sayre MH, et al.  (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Woychik NA, et al.  (1990) Subunits shared by eukaryotic nuclear RNA polymerases. Genes Dev 4(3):313-23	SGD
RPB8/YOR224C	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Kolodziej PA, et al.  (1990) RNA polymerase II subunit composition, stoichiometry, and phosphorylation. Mol Cell Biol 10(5):1915-20	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Sayre MH, et al.  (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Woychik NA, et al.  (1990) Subunits shared by eukaryotic nuclear RNA polymerases. Genes Dev 4(3):313-23	SGD
RPB9/YGL070C	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Kolodziej PA, et al.  (1990) RNA polymerase II subunit composition, stoichiometry, and phosphorylation. Mol Cell Biol 10(5):1915-20	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Sayre MH, et al.  (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Woychik NA, et al.  (1990) Subunits shared by eukaryotic nuclear RNA polymerases. Genes Dev 4(3):313-23	SGD
RPC10/YHR143W-A	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Sayre MH, et al.  (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-19	manually curated	Carles C, et al.  (1991) Two additional common subunits, ABC10 alpha and ABC10 beta, are shared by yeast RNA polymerases. J Biol Chem 266(35):24092-6	SGD
RPO21/YDL140C	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Kolodziej PA, et al.  (1990) RNA polymerase II subunit composition, stoichiometry, and phosphorylation. Mol Cell Biol 10(5):1915-20	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Sayre MH, et al.  (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Woychik NA, et al.  (1990) Subunits shared by eukaryotic nuclear RNA polymerases. Genes Dev 4(3):313-23	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR000684 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
RPO26/YPR187W	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Kolodziej PA, et al.  (1990) RNA polymerase II subunit composition, stoichiometry, and phosphorylation. Mol Cell Biol 10(5):1915-20	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Sayre MH, et al.  (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82	SGD
	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Woychik NA, et al.  (1990) Subunits shared by eukaryotic nuclear RNA polymerases. Genes Dev 4(3):313-23	SGD

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