Ontology: Molecular Function (GO:0004826)

Definition: Catalysis of the reaction: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

Synonyms: phenylalanine translase activity; phenylalanyl-tRNA synthetase activity; L-phenylalanine:tRNAPhe ligase (AMP-forming) activity; L-phenylalanyl-tRNA synthetase activity; phenylalanine-tRNA synthetase activity; phenylalanyl-transfer ribonucleate synthetase activity; phenylalanyl-transfer RNA ligase activity; phenylalanyl-transfer RNA synthetase activity; phenylalanyl-tRNA ligase activity

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term phenylalanine-tRNA ligase activity (3 genes) or to its variants containing one or more qualifiers (2 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	contributes_to: phenylalanine-tRNA ligase activity	2
	phenylalanine-tRNA ligase activity	1
High-throughput	none	none
Computational (download data)	phenylalanine-tRNA ligase activity	3

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term phenylalanine-tRNA ligase activity or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

phenylalanine-tRNA ligase activity      3 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
FRS1/YLR060W	IEA: Inferred from Electronic Annotation with EBI:IPR004531, EBI:IPR005146 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:6.1.1.20 Last Updated 2013-03-02	computational	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
FRS2/YFL022C	IEA: Inferred from Electronic Annotation with EBI:IPR004529 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:6.1.1.20 Last Updated 2013-03-02	computational	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
MSF1/YPR047W	IDA: Inferred from Direct Assay Assigned on 2007-01-19	manually curated	Sanni A, et al.  (1991) Evolution of aminoacyl-tRNA synthetase quaternary structure and activity: Saccharomyces cerevisiae mitochondrial phenylalanyl-tRNA synthetase. Proc Natl Acad Sci U S A 88(19):8387-91	MGI
	IMP: Inferred from Mutant Phenotype, ISS: Inferred from Sequence or structural Similarity Assigned on 2001-01-18	manually curated	Koerner TJ, et al.  (1987) Isolation and characterization of the yeast gene coding for the alpha subunit of mitochondrial phenylalanyl-tRNA synthetase. J Biol Chem 262(8):3690-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-12-05	manually curated	Roy H, et al.  (2005) Loss of editing activity during the evolution of mitochondrial phenylalanyl-tRNA synthetase. J Biol Chem 280(46):38186-92	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR004530, EBI:IPR005121 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with IUBMB:6.1.1.20 Last Updated 2013-03-02	computational	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB

contributes_to: phenylalanine-tRNA ligase activity      2 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
FRS1/YLR060W	IDA: Inferred from Direct Assay Assigned on 2008-12-05	manually curated	Roy H, et al.  (2005) Loss of editing activity during the evolution of mitochondrial phenylalanyl-tRNA synthetase. J Biol Chem 280(46):38186-92	SGD
	IDA: Inferred from Direct Assay Assigned on 2013-03-19	manually curated	Fasiolo F, et al.  (1975) Modification of phenylalanyl-tRNA synthetase from baker's yeast by proteolytic cleavage and properties of the trypsin-modified enzyme. Eur J Biochem 53(2):487-92	SGD
FRS2/YFL022C	IDA: Inferred from Direct Assay Assigned on 2008-12-05	manually curated	Roy H, et al.  (2005) Loss of editing activity during the evolution of mitochondrial phenylalanyl-tRNA synthetase. J Biol Chem 280(46):38186-92	SGD
	IDA: Inferred from Direct Assay Assigned on 2013-03-19	manually curated	Fasiolo F, et al.  (1975) Modification of phenylalanyl-tRNA synthetase from baker's yeast by proteolytic cleavage and properties of the trypsin-modified enzyme. Eur J Biochem 53(2):487-92	SGD

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