Ontology: Biological Process (GO:0000480)

Definition: Endonucleolytic cleavage within the 5'-External Transcribed Spacer (ETS) of a tricistronic rRNA transcript that contains the Small Subunit (SSU) rRNA, the 5.8S rRNA, and the Large Subunit (LSU) rRNA in that order from 5' to 3' along the primary transcript. Endonucleolytic cleavage within the 5'-ETS of the pre-RNA is conserved as one of the early steps of rRNA processing in all eukaryotes, but the specific position of cleavage is variable.

Synonyms: endonucleolytic cleavage at A-prime; endonucleolytic cleavage at A0

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (31 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	31
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)      31 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
DBP8/YHR169W	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-16	manually curated	Daugeron MC and Linder P  (2001) Characterization and mutational analysis of yeast Dbp8p, a putative RNA helicase involved in ribosome biogenesis. Nucleic Acids Res 29(5):1144-55	SGD
DIP2/YLR129W	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-03	manually curated	Gallagher JE, et al.  (2004) RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Genes Dev 18(20):2506-17	SGD
EMG1/YLR186W	IMP: Inferred from Mutant Phenotype Assigned on 2007-09-19	manually curated	Bernstein KA, et al.  (2004) The small-subunit processome is a ribosome assembly intermediate. Eukaryot Cell 3(6):1619-26	SGD
ESF2/YNR054C	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-16	manually curated	Hoang T, et al.  (2005) Esf2p, a U3-associated factor required for small-subunit processome assembly and compaction. Mol Cell Biol 25(13):5523-34	SGD
FCF1/YDR339C	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-03	manually curated	Rempola B, et al.  (2006) Fcf1p and Fcf2p are novel nucleolar Saccharomyces cerevisiae proteins involved in pre-rRNA processing. Biochem Biophys Res Commun 346(2):546-54	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2008-06-18	manually curated	Bleichert F, et al.  (2006) The PINc domain protein Utp24, a putative nuclease, is required for the early cleavage steps in 18S rRNA maturation. Proc Natl Acad Sci U S A 103(25):9464-9	SGD
FCF2/YLR051C	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-03	manually curated	Rempola B, et al.  (2006) Fcf1p and Fcf2p are novel nucleolar Saccharomyces cerevisiae proteins involved in pre-rRNA processing. Biochem Biophys Res Commun 346(2):546-54	SGD
LOC1/YFR001W	IMP: Inferred from Mutant Phenotype Assigned on 2009-03-12	manually curated	Urbinati CR, et al.  (2006) Loc1p is required for efficient assembly and nuclear export of the 60S ribosomal subunit. Mol Genet Genomics 276(4):369-77	SGD
MPP10/YJR002W	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-21	manually curated	Dunbar DA, et al.  (1997) Mpp10p, a U3 small nucleolar ribonucleoprotein component required for pre-18S rRNA processing in yeast. Mol Cell Biol 17(10):5803-12	SGD
MRD1/YPR112C	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-22	manually curated	Jin SB, et al.  (2002) Mrd1p is required for processing of pre-rRNA and for maintenance of steady-state levels of 40 S ribosomal subunits in yeast. J Biol Chem 277(21):18431-9	SGD
NOC4/YPR144C	IMP: Inferred from Mutant Phenotype Assigned on 2007-09-19	manually curated	Bernstein KA, et al.  (2004) The small-subunit processome is a ribosome assembly intermediate. Eukaryot Cell 3(6):1619-26	SGD
NOP14/YDL148C	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-03	manually curated	Gallagher JE, et al.  (2004) RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Genes Dev 18(20):2506-17	SGD
NOP19/YGR251W	IMP: Inferred from Mutant Phenotype Assigned on 2011-09-29	manually curated	Choque E, et al.  (2011) The nucleolar protein Nop19p interacts preferentially with Utp25p and Dhr2p and is essential for the production of the 40S ribosomal subunit in Saccharomyces cerevisiae. RNA Biol 8(6):1158-72	SGD
NOP58/YOR310C	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-13	manually curated	Lafontaine DL and Tollervey D  (1999) Nop58p is a common component of the box C+D snoRNPs that is required for snoRNA stability. RNA 5(3):455-67	SGD
NOP9/YJL010C	IMP: Inferred from Mutant Phenotype Assigned on 2008-09-02	manually curated	Thomson E, et al.  (2007) Nop9 is an RNA binding protein present in pre-40S ribosomes and required for 18S rRNA synthesis in yeast. RNA 13(12):2165-2174	SGD
PWP2/YCR057C	IMP: Inferred from Mutant Phenotype Assigned on 2007-09-18	manually curated	Dosil M and Bustelo XR  (2004) Functional characterization of Pwp2, a WD family protein essential for the assembly of the 90 S pre-ribosomal particle. J Biol Chem 279(36):37385-97	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2008-06-30	manually curated	Bernstein KA, et al.  (2004) The small-subunit processome is a ribosome assembly intermediate. Eukaryot Cell 3(6):1619-26	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-03	manually curated	Gallagher JE, et al.  (2004) RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Genes Dev 18(20):2506-17	SGD
RCL1/YOL010W	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-22	manually curated	Billy E, et al.  (2000) Rcl1p, the yeast protein similar to the RNA 3'-phosphate cyclase, associates with U3 snoRNP and is required for 18S rRNA biogenesis. EMBO J 19(9):2115-26	SGD
ROK1/YGL171W	IMP: Inferred from Mutant Phenotype Assigned on 2012-04-24	manually curated	Venema J, et al.  (1997) Rok1p is a putative RNA helicase required for rRNA processing. Mol Cell Biol 17(6):3398-407	SGD
RRP5/YMR229C	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-20	manually curated	Venema J and Tollervey D  (1996) RRP5 is required for formation of both 18S and 5.8S rRNA in yeast. EMBO J 15(20):5701-14	SGD
SAS10/YDL153C	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-03	manually curated	Gallagher JE, et al.  (2004) RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Genes Dev 18(20):2506-17	SGD
SNR17A/snR17a	IGI: Inferred from Genetic Interaction with SGD:ETS1-1, SGD:ETS1-2 Assigned on 2007-09-16	manually curated	Beltrame M and Tollervey D  (1995) Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis. EMBO J 14(17):4350-6	SGD
SNR17B/snR17b	IGI: Inferred from Genetic Interaction with SGD:ETS1-1, SGD:ETS1-2 Assigned on 2007-09-16	manually curated	Beltrame M and Tollervey D  (1995) Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis. EMBO J 14(17):4350-6	SGD
TOM1/YDR457W	IMP: Inferred from Mutant Phenotype Assigned on 2009-12-14	manually curated	Tabb AL, et al.  (2001) Genes encoding ribosomal proteins Rps0A/B of Saccharomyces cerevisiae interact with TOM1 mutants defective in ribosome synthesis. Genetics 157(3):1107-16	SGD
UTP10/YJL109C	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-30	manually curated	Dez C, et al.  (2007) Roles of the HEAT repeat proteins Utp10 and Utp20 in 40S ribosome maturation. RNA 13(9):1516-27	SGD
UTP11/YKL099C	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-03	manually curated	Gallagher JE, et al.  (2004) RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Genes Dev 18(20):2506-17	SGD
UTP13/YLR222C	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-03	manually curated	Gallagher JE, et al.  (2004) RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Genes Dev 18(20):2506-17	SGD
UTP14/YML093W	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-03	manually curated	Gallagher JE, et al.  (2004) RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Genes Dev 18(20):2506-17	SGD
UTP18/YJL069C	IMP: Inferred from Mutant Phenotype Assigned on 2007-09-19	manually curated	Bernstein KA, et al.  (2004) The small-subunit processome is a ribosome assembly intermediate. Eukaryot Cell 3(6):1619-26	SGD
UTP20/YBL004W	IMP: Inferred from Mutant Phenotype Assigned on 2007-09-19	manually curated	Bernstein KA, et al.  (2004) The small-subunit processome is a ribosome assembly intermediate. Eukaryot Cell 3(6):1619-26	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-07-30	manually curated	Dez C, et al.  (2007) Roles of the HEAT repeat proteins Utp10 and Utp20 in 40S ribosome maturation. RNA 13(9):1516-27	SGD
UTP23/YOR004W	IMP: Inferred from Mutant Phenotype Assigned on 2008-06-18	manually curated	Bleichert F, et al.  (2006) The PINc domain protein Utp24, a putative nuclease, is required for the early cleavage steps in 18S rRNA maturation. Proc Natl Acad Sci U S A 103(25):9464-9	SGD
UTP6/YDR449C	IMP: Inferred from Mutant Phenotype Assigned on 2007-08-03	manually curated	Gallagher JE, et al.  (2004) RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Genes Dev 18(20):2506-17	SGD

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