Ontology: Molecular Function (GO:0046961)

Definition: Catalysis of the transfer of protons from one side of a membrane to the other according to the reaction: ATP + H2O + H+(in) = ADP + phosphate + H+(out), by a rotational mechanism.

Synonyms: H(+)-transporting ATPase activity; hydrogen ion translocating F-type ATPase activity; hydrogen ion transporting two-sector ATPase activity; ATP synthase activity; chloroplast ATPase activity; F(0)F(1)-ATPase activity; F(1)-ATPase activity; F(o)F(1)-ATPase activity; H(+)-transporting ATP synthase activity; hydrogen ion translocating A-type ATPase activity; F0F1-ATPase; hydrogen ion translocating V-type ATPase activity; mitochondrial ATPase activity; ATP phosphohydrolase (H+-transporting) activity; bacterial Ca2+/Mg2+ ATPase; coupling factors (Fo, F1 and CF1); F1-ATPase; FoF1-ATPase; H+-transporting ATPase activity; H+-transporting two-sector ATPase; hydrogen ion transporting ATPase activity, rotational mechanism

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term proton-transporting ATPase activity, rotational mechanism (18 genes) or to its variants containing one or more qualifiers (16 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	contributes_to: proton-transporting ATPase activity, rotational mechanism	16
	proton-transporting ATPase activity, rotational mechanism	14
High-throughput	none	none
Computational (download data)	proton-transporting ATPase activity, rotational mechanism	7

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term proton-transporting ATPase activity, rotational mechanism or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

contributes_to: proton-transporting ATPase activity, rotational mechanism      16 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
ATP1/YBL099W	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD
ATP14/YLR295C	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD
ATP15/YPL271W	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD
ATP16/YDL004W	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD
ATP17/YDR377W	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD
ATP18/YML081C-A	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD
ATP2/YJR121W	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD
ATP20/YPR020W	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD
ATP3/YBR039W	IDA: Inferred from Direct Assay Assigned on 2011-05-27	manually curated	Ichikawa N and Mizuno M  (2004) Functional expression of hexahistidine-tagged beta-subunit of yeast F1-ATPase and isolation of the enzyme by immobilized metal affinity chromatography. Protein Expr Purif 37(1):97-101	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-27	manually curated	Paul MF, et al.  (1994) Cloning of the yeast ATP3 gene coding for the gamma-subunit of F1 and characterization of atp3 mutants. J Biol Chem 269(42):26158-64	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD
ATP4/YPL078C	IMP: Inferred from Mutant Phenotype Assigned on 2011-03-24	manually curated	Razaka-Jolly D, et al.  (1994) Mutation in the hydrophobic domain of ATP synthase subunit 4 (subunit b) of yeast mitochondria disturbs coupling between proton translocation and catalysis. Biochemistry 33(32):9684-91	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD
ATP5/YDR298C	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD
ATP6/Q0085	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD
ATP7/YKL016C	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD
ATP8/Q0080	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD
OLI1/Q0130	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD
TIM11/YDR322C-A	IDA: Inferred from Direct Assay Assigned on 2011-01-21	manually curated	Forster K, et al.  (2010) Proton transport coupled ATP synthesis by the purified yeast H(+)-ATP synthase in proteoliposomes. Biochim Biophys Acta 1797(11):1828-37	SGD

proton-transporting ATPase activity, rotational mechanism      18 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
ATP1/YBL099W	IEA: Inferred from Electronic Annotation with EBI:IPR005294 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
ATP15/YPL271W	IEA: Inferred from Electronic Annotation with EBI:IPR006721 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
ATP16/YDL004W	IEA: Inferred from Electronic Annotation with EBI:IPR001469, EBI:IPR020546 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
ATP2/YJR121W	IMP: Inferred from Mutant Phenotype Assigned on 2008-08-26	manually curated	Mueller DM  (1989) Site directed mutagenesis of the beta-subunit of the yeast mitochondrial ATPase. Biochem Biophys Res Commun 164(1):381-6	SGD
ATP3/YBR039W	IEA: Inferred from Electronic Annotation with EBI:IPR000131 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
STV1/YMR054W	IGI: Inferred from Genetic Interaction with SGD:VPH1, ISS: Inferred from Sequence or structural Similarity with SGD:VPH1 Assigned on 2006-04-11	manually curated	Manolson MF, et al.  (1994) STV1 gene encodes functional homologue of 95-kDa yeast vacuolar H(+)-ATPase subunit Vph1p. J Biol Chem 269(19):14064-74	SGD
VMA1/YDL185W	TAS: Traceable Author Statement Assigned on 2003-07-16	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2003-07-16	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VMA10/YHR039C-A	TAS: Traceable Author Statement Assigned on 2003-07-16	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2003-07-16	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VMA11/YPL234C	TAS: Traceable Author Statement Assigned on 2003-07-16	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2003-07-16	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VMA13/YPR036W	TAS: Traceable Author Statement Assigned on 2003-07-16	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2003-07-16	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR004908 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
VMA16/YHR026W	TAS: Traceable Author Statement Assigned on 2003-07-16	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2003-07-16	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VMA2/YBR127C	IMP: Inferred from Mutant Phenotype Assigned on 2006-10-16	manually curated	Yamashiro CT, et al.  (1990) Role of vacuolar acidification in protein sorting and zymogen activation: a genetic analysis of the yeast vacuolar proton-translocating ATPase. Mol Cell Biol 10(7):3737-49	SGD
VMA4/YOR332W	TAS: Traceable Author Statement Assigned on 2003-07-10	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2003-07-10	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR002842 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
VMA5/YKL080W	TAS: Traceable Author Statement Assigned on 2003-07-10	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2003-07-10	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VMA6/YLR447C	IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2003-07-10	manually curated	Bauerle C, et al.  (1993) The Saccharomyces cerevisiae VMA6 gene encodes the 36-kDa subunit of the vacuolar H(+)-ATPase membrane sector. J Biol Chem 268(17):12749-57	SGD
VMA7/YGR020C	TAS: Traceable Author Statement Assigned on 2003-07-10	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2003-07-10	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR005772 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
VMA8/YEL051W	TAS: Traceable Author Statement Assigned on 2003-07-10	manually curated	Forgac M  (1999) Structure and properties of the vacuolar (H+)-ATPases. J Biol Chem 274(19):12951-4	SGD
	TAS: Traceable Author Statement Assigned on 2003-07-10	manually curated	Stevens TH and Forgac M  (1997) Structure, function and regulation of the vacuolar (H+)-ATPase. Annu Rev Cell Dev Biol 13:779-808	SGD
VPH1/YOR270C	IDA: Inferred from Direct Assay Assigned on 2006-10-19	manually curated	Leng XH, et al.  (1996) Site-directed mutagenesis of the 100-kDa subunit (Vph1p) of the yeast vacuolar (H+)-ATPase. J Biol Chem 271(37):22487-93	SGD
	IDA: Inferred from Direct Assay Assigned on 2006-10-19	manually curated	Manolson MF, et al.  (1992) The VPH1 gene encodes a 95-kDa integral membrane polypeptide required for in vivo assembly and activity of the yeast vacuolar H(+)-ATPase. J Biol Chem 267(20):14294-303	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-04-27	manually curated	Su Y, et al.  (2008) Human H+ATPase a4 subunit mutations causing renal tubular acidosis reveal a role for interaction with phosphofructokinase-1. Am J Physiol Renal Physiol 295(4):F950-8	UniProtKB

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