Ontology: Molecular Function (GO:0004520)

Definition: Catalysis of the hydrolysis of ester linkages within deoxyribonucleic acid by creating internal breaks.

Synonyms: endonuclease G activity; DNA nicking activity

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term endodeoxyribonuclease activity (5 genes) or to its variants containing one or more qualifiers (1 gene). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	endodeoxyribonuclease activity	5
	NOT: endodeoxyribonuclease activity	1
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term endodeoxyribonuclease activity or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

NOT: endodeoxyribonuclease activity      1 gene directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
MRS1/YIR021W	IDA: Inferred from Direct Assay, ISS: Inferred from Sequence or structural Similarity with SGD:CCE1 Assigned on 2003-08-21	manually curated	Bassi GS, et al.  (2002) Recruitment of intron-encoded and co-opted proteins in splicing of the bI3 group I intron RNA. Proc Natl Acad Sci U S A 99(1):128-33	SGD

endodeoxyribonuclease activity      5 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
CCE1/YKL011C	IDA: Inferred from Direct Assay Assigned on 2002-11-05	manually curated	Ezekiel UR and Zassenhaus HP  (1993) Localization of a cruciform cutting endonuclease to yeast mitochondria. Mol Gen Genet 240(3):414-8	SGD
ENS2	IDA: Inferred from Direct Assay Assigned on 2003-03-04	manually curated	Mizumura H, et al.  (1999) Stable association of 70-kDa heat shock protein induces latent multisite specificity of a unisite-specific endonuclease in yeast mitochondria. J Biol Chem 274(36):25682-90	SGD
MRE11/YMR224C	IDA: Inferred from Direct Assay Assigned on 2005-09-19	manually curated	Ghosal G and Muniyappa K  (2005) Saccharomyces cerevisiae Mre11 is a high-affinity G4 DNA-binding protein and a G-rich DNA-specific endonuclease: implications for replication of telomeric DNA. Nucleic Acids Res 33(15):4692-703	SGD
NUC1/YJL208C	IDA: Inferred from Direct Assay Assigned on 2001-01-18	manually curated	Dake E, et al.  (1988) Purification and properties of the major nuclease from mitochondria of Saccharomyces cerevisiae. J Biol Chem 263(16):7691-702	SGD
VMA1/YDL185W	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Gimble FS and Thorner J  (1992) Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae. Nature 357(6376):301-6	SGD

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