Ontology: Molecular Function (GO:0004519)

Definition: Catalysis of the hydrolysis of ester linkages within nucleic acids by creating internal breaks.

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term endonuclease activity (91 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	endonuclease activity	13
High-throughput	none	none
Computational (download data)	endonuclease activity	86

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term endonuclease activity or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

endonuclease activity      91 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
AI1/Q0050	TAS: Traceable Author Statement Assigned on 2002-10-24	manually curated	Eskes R, et al.  (1997) Mobility of yeast mitochondrial group II introns: engineering a new site specificity and retrohoming via full reverse splicing. Cell 88(6):865-74	SGD
AI2/Q0055	TAS: Traceable Author Statement Assigned on 2002-10-24	manually curated	Eskes R, et al.  (1997) Mobility of yeast mitochondrial group II introns: engineering a new site specificity and retrohoming via full reverse splicing. Cell 88(6):865-74	SGD
AI3/Q0060	IDA: Inferred from Direct Assay Assigned on 2002-11-18	manually curated	Guo WW, et al.  (1995) The mobile group I intron 3 alpha of the yeast mitochondrial COXI gene encodes a 35-kDa processed protein that is an endonuclease but not a maturase. J Biol Chem 270(26):15563-70	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR001982 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
AI4/Q0065	IDA: Inferred from Direct Assay Assigned on 2002-11-14	manually curated	Henke RM, et al.  (1995) Maturase and endonuclease functions depend on separate conserved domains of the bifunctional protein encoded by the group I intron aI4 alpha of yeast mitochondrial DNA. EMBO J 14(20):5094-9	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR001982 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
AI5_ALPHA/Q0070	IMP: Inferred from Mutant Phenotype Assigned on 2002-11-19	manually curated	Seraphin B, et al.  (1992) The yeast mitochondrial intron aI5 alpha: associated endonuclease activity and in vivo mobility. Gene 113(1):1-8	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR004860 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
AI5_BETA/Q0075	IEA: Inferred from Electronic Annotation with EBI:IPR001982 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
APN1/YKL114C	IEA: Inferred from Electronic Annotation with EBI:IPR018246 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
APN2/YBL019W	IEA: Inferred from Electronic Annotation with EBI:IPR020848 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
BI2/Q0110	IEA: Inferred from Electronic Annotation with EBI:IPR004860 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
BI3/Q0115	IEA: Inferred from Electronic Annotation with EBI:IPR001982 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
BI4/Q0120	IEA: Inferred from Electronic Annotation with EBI:IPR001982 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
CCE1/YKL011C	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DIN7/YDR263C	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DIS3/YOL021C	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
DOM34/YNL001W	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
HO/YDL227C	IDA: Inferred from Direct Assay Assigned on 2002-07-24	manually curated	Kostriken R, et al.  (1983) A site-specific endonuclease essential for mating-type switching in Saccharomyces cerevisiae. Cell 35(1):167-74	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR007869, EBI:IPR004042 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on automatic assignment of UniProtKB keywords in UniProtKB/TrEMBL entries.	UniProtKB
LCL3/YGL085W	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
MMS4/YBR098W	IDA: Inferred from Direct Assay Assigned on 2002-10-01	manually curated	Kaliraman V, et al.  (2001) Functional overlap between Sgs1-Top3 and the Mms4-Mus81 endonuclease. Genes Dev 15(20):2730-40	SGD
	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
MRE11/YMR224C	IDA: Inferred from Direct Assay Assigned on 2001-01-18	manually curated	Moreau S, et al.  (1999) The nuclease activity of Mre11 is required for meiosis but not for mating type switching, end joining, or telomere maintenance. Mol Cell Biol 19(1):556-66	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR007281 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
MUS81/YDR386W	IDA: Inferred from Direct Assay Assigned on 2002-10-01	manually curated	Kaliraman V, et al.  (2001) Functional overlap between Sgs1-Top3 and the Mms4-Mus81 endonuclease. Genes Dev 15(20):2730-40	SGD
	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
NGL1/YOL042W	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-12-16	manually curated	Faber AW, et al.  (2002) Ngl2p is a Ccr4p-like RNA nuclease essential for the final step in 3'-end processing of 5.8S rRNA in Saccharomyces cerevisiae. RNA 8(9):1095-101	SGD
NGL3/YML118W	ISS: Inferred from Sequence or structural Similarity Assigned on 2002-12-16	manually curated	Faber AW, et al.  (2002) Ngl2p is a Ccr4p-like RNA nuclease essential for the final step in 3'-end processing of 5.8S rRNA in Saccharomyces cerevisiae. RNA 8(9):1095-101	SGD
NTG1/YAL015C	IEA: Inferred from Electronic Annotation with EBI:IPR004036 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
NTG2/YOL043C	IEA: Inferred from Electronic Annotation with EBI:IPR004036, EBI:IPR003651 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
NUC1/YJL208C	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
Q0255	IEA: Inferred from Electronic Annotation with EBI:IPR001982 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
RAD1/YPL022W	IEA: Inferred from Electronic Annotation with EBI:IPR006167 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
RAD10/YML095C	IEA: Inferred from Electronic Annotation with EBI:IPR004579 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
RAD2/YGR258C	IEA: Inferred from Electronic Annotation with EBI:IPR001044 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
RAD27/YKL113C	IEA: Inferred from Electronic Annotation with EBI:KW-0255 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

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