Ontology: Biological Process (GO:0000398)

Definition: The joining together of exons from one or more primary transcripts of messenger RNA (mRNA) and the excision of intron sequences, via a spliceosomal mechanism, so that mRNA consisting only of the joined exons is produced.

Synonyms: mRNA splicing; pre-mRNA splicing; splicing GT-AG intron; splicing AT-AC intron; nuclear mRNA splicing via U12-type spliceosome; nuclear mRNA splicing via U2-type spliceosome; nuclear mRNA splicing, via spliceosome

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term mRNA splicing, via spliceosome (85 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	mRNA splicing, via spliceosome	81
High-throughput (download data)	mRNA splicing, via spliceosome	3
Computational (download data)	mRNA splicing, via spliceosome	9

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term mRNA splicing, via spliceosome or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

mRNA splicing, via spliceosome      85 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
SNR6/snR6	IPI: Inferred from Physical Interaction, ISS: Inferred from Sequence or structural Similarity Assigned on 2002-11-18	manually curated	Siliciano PG, et al.  (1987) An essential snRNA from S. cerevisiae has properties predicted for U4, including interaction with a U6-like snRNA. Cell 50(4):585-92	SGD
SNR7-L/snR7-L	IMP: Inferred from Mutant Phenotype Assigned on 2002-07-17	manually curated	Patterson B and Guthrie C  (1987) An essential yeast snRNA with a U5-like domain is required for splicing in vivo. Cell 49(5):613-24	SGD
SNR7-S/snR7-S	IMP: Inferred from Mutant Phenotype Assigned on 2002-07-17	manually curated	Patterson B and Guthrie C  (1987) An essential yeast snRNA with a U5-like domain is required for splicing in vivo. Cell 49(5):613-24	SGD
SNT309/YPR101W	IGI: Inferred from Genetic Interaction with SGD:PRP19, IMP: Inferred from Mutant Phenotype Assigned on 2005-09-23	manually curated	Chen HR, et al.  (1998) Snt309p, a component of the Prp19p-associated complex that interacts with Prp19p and associates with the spliceosome simultaneously with or immediately after dissociation of U4 in the same manner as Prp19p. Mol Cell Biol 18(4):2196-204	SGD
SNU114/YKL173W	IMP: Inferred from Mutant Phenotype Assigned on 2005-10-06	manually curated	Fabrizio P, et al.  (1997) An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2. EMBO J 16(13):4092-106	SGD
SNU13/YEL026W	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-10-04	manually curated	Stevens SW, et al.  (2001) Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae. RNA 7(11):1543-53	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-02-23	manually curated	Dobbyn HC and O'Keefe RT  (2004) Analysis of Snu13p mutations reveals differential interactions with the U4 snRNA and U3 snoRNA. RNA 10(2):308-20	SGD
SNU23/YDL098C	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-10-04	manually curated	Stevens SW, et al.  (2001) Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae. RNA 7(11):1543-53	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-10-03	manually curated	Gottschalk A, et al.  (1999) Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP. EMBO J 18(16):4535-48	SGD
SNU56/YDR240C	IPI: Inferred from Physical Interaction Assigned on 2002-07-29	manually curated	Zhang D and Rosbash M  (1999) Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. Genes Dev 13(5):581-92	SGD
SNU66/YOR308C	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-10-04	manually curated	Stevens SW, et al.  (2001) Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae. RNA 7(11):1543-53	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-10-03	manually curated	Gottschalk A, et al.  (1999) Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP. EMBO J 18(16):4535-48	SGD
SNU71/YGR013W	IPI: Inferred from Physical Interaction Assigned on 2002-10-30	manually curated	Gottschalk A, et al.  (1998) A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins. RNA 4(4):374-93	SGD
SPP2/YOR148C	IMP: Inferred from Mutant Phenotype Assigned on 2002-11-04	manually curated	Roy J, et al.  (1995) The final stages of spliceosome maturation require Spp2p that can interact with the DEAH box protein Prp2p and promote step 1 of splicing. RNA 1(4):375-90	SGD
SPP381/YBR152W	IMP: Inferred from Mutant Phenotype Assigned on 2005-09-23	manually curated	Lybarger S, et al.  (1999) Elevated levels of a U4/U6.U5 snRNP-associated protein, Spp381p, rescue a mutant defective in spliceosome maturation. Mol Cell Biol 19(1):577-84	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-09-26	manually curated	Gottschalk A, et al.  (1999) Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP. EMBO J 18(16):4535-48	SGD
SPP382/YLR424W	IPI: Inferred from Physical Interaction Assigned on 2003-12-30	high-throughput	Hazbun TR, et al.  (2003) Assigning function to yeast proteins by integration of technologies. Mol Cell 12(6):1353-65	SGD
SPT4/YGR063C	IMP: Inferred from Mutant Phenotype Assigned on 2012-02-24	manually curated	Lindstrom DL, et al.  (2003) Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol Cell Biol 23(4):1368-78	SGD
SPT5/YML010W	IMP: Inferred from Mutant Phenotype Assigned on 2012-02-24	manually curated	Lindstrom DL, et al.  (2003) Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol Cell Biol 23(4):1368-78	SGD
SQS1/YNL224C	IGI: Inferred from Genetic Interaction with SGD:SPP382 Assigned on 2007-08-24	manually curated	Pandit S, et al.  (2006) Inhibition of a spliceosome turnover pathway suppresses splicing defects. Proc Natl Acad Sci U S A 103(37):13700-5	SGD
STO1/YMR125W	IPI: Inferred from Physical Interaction Assigned on 2002-07-29	manually curated	Zhang D and Rosbash M  (1999) Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. Genes Dev 13(5):581-92	SGD
SUB2/YDL084W	IMP: Inferred from Mutant Phenotype Assigned on 2005-07-11	manually curated	Zhang M and Green MR  (2001) Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56. Genes Dev 15(1):30-5	SGD
SWT21/YNL187W	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-25	manually curated	Hage R, et al.  (2009) A targeted bypass screen identifies Ynl187p, Prp42p, Snu71p, and Cbp80p for stable U1 snRNP/Pre-mRNA interaction. Mol Cell Biol 29(14):3941-52	SGD
SYF2/YGR129W	IMP: Inferred from Mutant Phenotype Assigned on 2009-10-20	manually curated	Russell CS, et al.  (2000) Functional analyses of interacting factors involved in both pre-mRNA splicing and cell cycle progression in Saccharomyces cerevisiae. RNA 6(11):1565-72	SGD
THP3/YPR045C	IMP: Inferred from Mutant Phenotype Assigned on 2009-10-07	manually curated	Wilmes GM, et al.  (2008) A genetic interaction map of RNA-processing factors reveals links between Sem1/Dss1-containing complexes and mRNA export and splicing. Mol Cell 32(5):735-46	SGD
USA1/YML029W	IPI: Inferred from Physical Interaction with SGD:SNP1 Assigned on 2006-11-15	manually curated	Awasthi S, et al.  (2001) New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing. J Biol Chem 276(33):31004-15	SGD
YHC1/YLR298C	IPI: Inferred from Physical Interaction Assigned on 2002-07-29	manually curated	Zhang D and Rosbash M  (1999) Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. Genes Dev 13(5):581-92	SGD
YJR084W	IMP: Inferred from Mutant Phenotype Assigned on 2009-10-07	manually curated	Wilmes GM, et al.  (2008) A genetic interaction map of RNA-processing factors reveals links between Sem1/Dss1-containing complexes and mRNA export and splicing. Mol Cell 32(5):735-46	SGD
YSF3/YNL138W-A	IPI: Inferred from Physical Interaction Assigned on 2008-10-27	manually curated	Wang Q, et al.  (2005) Interactions of the yeast SF3b splicing factor. Mol Cell Biol 25(24):10745-54	SGD

SGD Home About Blog Site Map Help Terms of Use
© Stanford University, Stanford, CA 94305.