Ontology: Biological Process (GO:0000398)

Definition: The joining together of exons from one or more primary transcripts of messenger RNA (mRNA) and the excision of intron sequences, via a spliceosomal mechanism, so that mRNA consisting only of the joined exons is produced.

Synonyms: mRNA splicing; pre-mRNA splicing; splicing GT-AG intron; splicing AT-AC intron; nuclear mRNA splicing via U12-type spliceosome; nuclear mRNA splicing via U2-type spliceosome; nuclear mRNA splicing, via spliceosome

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term mRNA splicing, via spliceosome (85 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	mRNA splicing, via spliceosome	81
High-throughput (download data)	mRNA splicing, via spliceosome	3
Computational (download data)	mRNA splicing, via spliceosome	9

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term mRNA splicing, via spliceosome or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

mRNA splicing, via spliceosome      85 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
NAM8/YHR086W	IPI: Inferred from Physical Interaction Assigned on 2002-07-29	manually curated	Zhang D and Rosbash M  (1999) Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. Genes Dev 13(5):581-92	SGD
NPL3/YDR432W	IGI: Inferred from Genetic Interaction with SGD:NAM8, SGD:SNU66, SGD:CUS2, SGD:LEA1, IMP: Inferred from Mutant Phenotype Assigned on 2008-12-17	manually curated	Kress TL, et al.  (2008) A Single SR-like Protein, Npl3, Promotes Pre-mRNA Splicing in Budding Yeast. Mol Cell 32(5):727-34	SGD
NTC20/YBR188C	IGI: Inferred from Genetic Interaction with SGD:ISY1 Assigned on 2001-01-18	manually curated	Chen CH, et al.  (2001) Identification and characterization of two novel components of the Prp19p-associated complex, Ntc30p and Ntc20p. J Biol Chem 276(1):488-94	SGD
NTR2/YKR022C	IPI: Inferred from Physical Interaction with SGD:PRP8, SGD:CWC2 Assigned on 2003-12-30	high-throughput	Hazbun TR, et al.  (2003) Assigning function to yeast proteins by integration of technologies. Mol Cell 12(6):1353-65	SGD
PML1/YLR016C	IDA: Inferred from Direct Assay Assigned on 2005-10-06	manually curated	Dziembowski A, et al.  (2004) Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing. EMBO J 23(24):4847-56	SGD
PRP18/YGR006W	IMP: Inferred from Mutant Phenotype Assigned on 2003-01-10	manually curated	Bacikova D and Horowitz DS  (2002) Mutational analysis identifies two separable roles of the Saccharomyces cerevisiae splicing factor Prp18. RNA 8(10):1280-93	SGD
PRP21/YJL203W	IDA: Inferred from Direct Assay Assigned on 2002-11-14	manually curated	Wiest DK, et al.  (1996) In vitro studies of the Prp9.Prp11.Prp21 complex indicate a pathway for U2 small nuclear ribonucleoprotein activation. J Biol Chem 271(52):33268-76	SGD
PRP3/YDR473C	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR027104 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
PRP31/YGR091W	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR027105 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
PRP4/YPR178W	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
PRP40/YKL012W	IDA: Inferred from Direct Assay Assigned on 2002-11-14	manually curated	Kao HY and Siliciano PG  (1996) Identification of Prp40, a novel essential yeast splicing factor associated with the U1 small nuclear ribonucleoprotein particle. Mol Cell Biol 16(3):960-7	SGD
PRP42/YDR235W	IMP: Inferred from Mutant Phenotype Assigned on 2002-11-04	manually curated	McLean MR and Rymond BC  (1998) Yeast pre-mRNA splicing requires a pair of U1 snRNP-associated tetratricopeptide repeat proteins. Mol Cell Biol 18(1):353-60	SGD
PRP45/YAL032C	IEA: Inferred from Electronic Annotation with EBI:IPR017862, EBI:IPR004015 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
PRP46/YPL151C	IMP: Inferred from Mutant Phenotype Assigned on 2006-09-18	manually curated	Albers M, et al.  (2003) Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors. RNA 9(1):138-50	SGD
PRP6/YBR055C	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR010491 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
PRP8/YHR165C	IEA: Inferred from Electronic Annotation with EBI:IPR012592, EBI:IPR012591 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
PRP9/YDL030W	IDA: Inferred from Direct Assay Assigned on 2002-11-14	manually curated	Wiest DK, et al.  (1996) In vitro studies of the Prp9.Prp11.Prp21 complex indicate a pathway for U2 small nuclear ribonucleoprotein activation. J Biol Chem 271(52):33268-76	SGD
RDS3/YPR094W	IGI: Inferred from Genetic Interaction with SGD:CLF1, IMP: Inferred from Mutant Phenotype Assigned on 2005-03-10	manually curated	Vincent K, et al.  (2003) Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing. Genetics 164(3):895-907	SGD
	IPI: Inferred from Physical Interaction Assigned on 2008-10-27	manually curated	Wang Q, et al.  (2005) Interactions of the yeast SF3b splicing factor. Mol Cell Biol 25(24):10745-54	SGD
RSE1/YML049C	IPI: Inferred from Physical Interaction Assigned on 2008-10-27	manually curated	Wang Q, et al.  (2005) Interactions of the yeast SF3b splicing factor. Mol Cell Biol 25(24):10745-54	SGD
	IGI: Inferred from Genetic Interaction, IMP: Inferred from Mutant Phenotype Assigned on 2002-10-01	manually curated	Chen EJ, et al.  (1998) A link between secretion and pre-mRNA processing defects in Saccharomyces cerevisiae and the identification of a novel splicing gene, RSE1. Mol Cell Biol 18(12):7139-46	SGD
SAD1/YFR005C	IDA: Inferred from Direct Assay Assigned on 2002-08-22	manually curated	Lygerou Z, et al.  (1999) A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing. Mol Cell Biol 19(3):2008-20	SGD
SMB1/YER029C	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
SMD1/YGR074W	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-01-19	manually curated	Rymond BC  (1993) Convergent transcripts of the yeast PRP38-SMD1 locus encode two essential splicing factors, including the D1 core polypeptide of small nuclear ribonucleoprotein particles. Proc Natl Acad Sci U S A 90(3):848-52	UniProtKB
	IPI: Inferred from Physical Interaction Assigned on 2002-07-29	manually curated	Zhang D and Rosbash M  (1999) Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. Genes Dev 13(5):581-92	SGD
SMD2/YLR275W	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
SMD3/YLR147C	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
	IPI: Inferred from Physical Interaction Assigned on 2002-07-29	manually curated	Zhang D and Rosbash M  (1999) Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. Genes Dev 13(5):581-92	SGD
SME1/YOR159C	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR027078 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
SMX2/YFL017W-A	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
SMX3/YPR182W	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
SNP1/YIL061C	IPI: Inferred from Physical Interaction Assigned on 2002-07-29	manually curated	Zhang D and Rosbash M  (1999) Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. Genes Dev 13(5):581-92	SGD
SNR14/snR14	TAS: Traceable Author Statement Assigned on 2002-07-08	manually curated	Hu J, et al.  (1995) Mutational analysis of Saccharomyces cerevisiae U4 small nuclear RNA identifies functionally important domains. Mol Cell Biol 15(3):1274-85	SGD
SNR19/snR19	IDA: Inferred from Direct Assay Assigned on 2002-07-15	manually curated	Kretzner L, et al.  (1987) S. cerevisiae U1 RNA is large and has limited primary sequence homology to metazoan U1 snRNA. Cell 50(4):593-602	SGD

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