Ontology: Biological Process (GO:0000398)

Definition: The joining together of exons from one or more primary transcripts of messenger RNA (mRNA) and the excision of intron sequences, via a spliceosomal mechanism, so that mRNA consisting only of the joined exons is produced.

Synonyms: mRNA splicing; pre-mRNA splicing; splicing GT-AG intron; splicing AT-AC intron; nuclear mRNA splicing via U12-type spliceosome; nuclear mRNA splicing via U2-type spliceosome; nuclear mRNA splicing, via spliceosome

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term mRNA splicing, via spliceosome (85 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	mRNA splicing, via spliceosome	81
High-throughput (download data)	mRNA splicing, via spliceosome	3
Computational (download data)	mRNA splicing, via spliceosome	9

Links to Additional Annotations:

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• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term mRNA splicing, via spliceosome or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

mRNA splicing, via spliceosome      85 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
BUD13/YGL174W	IDA: Inferred from Direct Assay Assigned on 2005-10-06	manually curated	Dziembowski A, et al.  (2004) Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing. EMBO J 23(24):4847-56	SGD
BUD31/YCR063W	IPI: Inferred from Physical Interaction Assigned on 2008-10-27	manually curated	Wang Q, et al.  (2005) Interactions of the yeast SF3b splicing factor. Mol Cell Biol 25(24):10745-54	SGD
CBC2/YPL178W	IMP: Inferred from Mutant Phenotype Assigned on 2002-07-30	manually curated	Colot HV, et al.  (1996) The yeast splicing factor Mud13p is a commitment complex component and corresponds to CBP20, the small subunit of the nuclear cap-binding complex. Genes Dev 10(13):1699-708	SGD
CEF1/YMR213W	IMP: Inferred from Mutant Phenotype Assigned on 2009-11-10	manually curated	Tsai WY, et al.  (1999) Cef1p is a component of the Prp19p-associated complex and essential for pre-mRNA splicing. J Biol Chem 274(14):9455-62	SGD
CUS1/YMR240C	IPI: Inferred from Physical Interaction Assigned on 2008-10-27	manually curated	Wang Q, et al.  (2005) Interactions of the yeast SF3b splicing factor. Mol Cell Biol 25(24):10745-54	SGD
CUS2/YNL286W	IGI: Inferred from Genetic Interaction, IPI: Inferred from Physical Interaction Assigned on 2002-09-26	manually curated	Yan D, et al.  (1998) CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif. Mol Cell Biol 18(9):5000-9	SGD
CWC15/YDR163W	IGI: Inferred from Genetic Interaction with SGD:PRP19 Assigned on 2004-04-29	manually curated	Ohi MD, et al.  (2002) Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Mol Cell Biol 22(7):2011-24	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR006973 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
CWC2/YDL209C	IMP: Inferred from Mutant Phenotype Assigned on 2002-09-05	manually curated	Ohi MD and Gould KL  (2002) Characterization of interactions among the Cef1p-Prp19p-associated splicing complex. RNA 8(6):798-815	SGD
CWC21/YDR482C	IGI: Inferred from Genetic Interaction with SGD:ISY1, SGD:BUD13, IMP: Inferred from Mutant Phenotype Assigned on 2010-07-26	manually curated	Khanna M, et al.  (2009) A systematic characterization of Cwc21, the yeast ortholog of the human spliceosomal protein SRm300. RNA 15(12):2174-85	SGD
CWC22/YGR278W	IDA: Inferred from Direct Assay Assigned on 2011-02-11	manually curated	Yeh TC, et al.  (2011) Splicing factor Cwc22 is required for the function of Prp2 and for the spliceosome to escape from a futile pathway. Mol Cell Biol 31(1):43-53	SGD
CWC25/YNL245C	IDA: Inferred from Direct Assay Assigned on 2009-12-03	manually curated	Warkocki Z, et al.  (2009) Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components. Nat Struct Mol Biol 16(12):1237-43	SGD
	IPI: Inferred from Physical Interaction with SGD:PRP19 Assigned on 2003-12-31	high-throughput	Hazbun TR, et al.  (2003) Assigning function to yeast proteins by integration of technologies. Mol Cell 12(6):1353-65	SGD
DIB1/YPR082C	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-10-04	manually curated	Stevens SW, et al.  (2001) Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae. RNA 7(11):1543-53	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2003-01-29	manually curated	Reuter K, et al.  (1999) Identification, characterization and crystal structure analysis of the human spliceosomal U5 snRNP-specific 15 kD protein. J Mol Biol 294(2):515-25	SGD
ECM2/YBR065C	IGI: Inferred from Genetic Interaction with SGD:LSR1 Assigned on 2004-02-17	manually curated	Xu D, et al.  (1998) Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing. Mol Cell Biol 18(4):2055-66	SGD
HSH155/YMR288W	IPI: Inferred from Physical Interaction Assigned on 2008-10-27	manually curated	Wang Q, et al.  (2005) Interactions of the yeast SF3b splicing factor. Mol Cell Biol 25(24):10745-54	SGD
HSH49/YOR319W	IPI: Inferred from Physical Interaction Assigned on 2008-10-27	manually curated	Wang Q, et al.  (2005) Interactions of the yeast SF3b splicing factor. Mol Cell Biol 25(24):10745-54	SGD
	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction Assigned on 2002-11-05	manually curated	Igel H, et al.  (1998) Conservation of structure and subunit interactions in yeast homologues of splicing factor 3b (SF3b) subunits. RNA 4(1):1-10	SGD
HUB1/YNR032C-A	IPI: Inferred from Physical Interaction with SGD:SNU66, ISO: Inferred from Sequence Orthology with EBI:O94650 Assigned on 2008-04-16	manually curated	Wilkinson CR, et al.  (2004) Ubiquitin-like protein Hub1 is required for pre-mRNA splicing and localization of an essential splicing factor in fission yeast. Curr Biol 14(24):2283-8	SGD
IST3/YIR005W	IDA: Inferred from Direct Assay Assigned on 2005-10-06	manually curated	Dziembowski A, et al.  (2004) Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing. EMBO J 23(24):4847-56	SGD
	IPI: Inferred from Physical Interaction Assigned on 2008-10-27	manually curated	Wang Q, et al.  (2005) Interactions of the yeast SF3b splicing factor. Mol Cell Biol 25(24):10745-54	SGD
LEA1/YPL213W	IMP: Inferred from Mutant Phenotype Assigned on 2009-10-15	manually curated	Caspary F and Seraphin B  (1998) The yeast U2A'/U2B complex is required for pre-spliceosome formation. EMBO J 17(21):6348-58	SGD
LSM2/YBL026W	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
LSM3/YLR438C-A	IPI: Inferred from Physical Interaction Assigned on 2003-01-13	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
	TAS: Traceable Author Statement Assigned on 2005-02-22	manually curated	Fernandez CF, et al.  (2004) An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5. Mol Biol Cell 15(6):2842-52	SGD
LSM4/YER112W	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
	TAS: Traceable Author Statement Assigned on 2005-02-22	manually curated	Fernandez CF, et al.  (2004) An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5. Mol Biol Cell 15(6):2842-52	SGD
LSM5/YER146W	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
LSM6/YDR378C	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
LSM7/YNL147W	IMP: Inferred from Mutant Phenotype Assigned on 2002-07-15	manually curated	Pannone BK, et al.  (2001) Multiple functional interactions between components of the Lsm2-Lsm8 complex, U6 snRNA, and the yeast La protein. Genetics 158(1):187-96	SGD
LSM8/YJR022W	IPI: Inferred from Physical Interaction Assigned on 2002-06-20	manually curated	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
LSR1	IPI: Inferred from Physical Interaction Assigned on 2002-11-18	manually curated	Xu D and Friesen JD  (2001) Splicing factor slt11p and its involvement in formation of U2/U6 helix II in activation of the yeast spliceosome. Mol Cell Biol 21(4):1011-23	SGD
MRN1/YPL184C	IGI: Inferred from Genetic Interaction with SGD:SNT309, SGD:PRP4, SGD:PRP22 Assigned on 2013-01-30	manually curated	During L, et al.  (2012) MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation. PLoS One 7(9):e44373	SGD
MSL1/YIR009W	IMP: Inferred from Mutant Phenotype Assigned on 2009-10-15	manually curated	Caspary F and Seraphin B  (1998) The yeast U2A'/U2B complex is required for pre-spliceosome formation. EMBO J 17(21):6348-58	SGD
	IPI: Inferred from Physical Interaction, ISS: Inferred from Sequence or structural Similarity Assigned on 2002-11-01	manually curated	Tang J, et al.  (1996) Identification and characterization of a yeast gene encoding the U2 small nuclear ribonucleoprotein particle B" protein. Mol Cell Biol 16(6):2787-95	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR024888 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
MSL5/YLR116W	IPI: Inferred from Physical Interaction Assigned on 2002-11-18	manually curated	Berglund JA, et al.  (1997) The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC. Cell 89(5):781-7	SGD
MUD1/YBR119W	IGI: Inferred from Genetic Interaction Assigned on 2002-11-15	manually curated	Liao XC, et al.  (1993) An enhancer screen identifies a gene that encodes the yeast U1 snRNP A protein: implications for snRNP protein function in pre-mRNA splicing. Genes Dev 7(3):419-28	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR024888 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

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