Ontology: Molecular Function (GO:0003887)

Definition: Catalysis of the reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1); the synthesis of DNA from deoxyribonucleotide triphosphates in the presence of a DNA template and primer.

Synonyms: DNA nucleotidyltransferase (DNA-directed) activity; DNA-dependent DNA polymerase activity; deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (DNA-directed) activity; deoxyribonucleic acid duplicase activity; deoxyribonucleic duplicase activity; deoxyribonucleic polymerase I; DNA duplicase activity; DNA polymerase I; DNA polymerase II; DNA polymerase III; DNA replicase activity; duplicase; Klenow fragment; sequenase; Taq DNA polymerase; Taq Pol I; Tca DNA polymerase; DNA polymerase alpha; DNA polymerase beta; DNA polymerase gamma; alpha DNA polymerase activity; beta DNA polymerase activity; delta DNA polymerase activity; DNA polymerase V activity; epsilon DNA polymerase activity; eta DNA polymerase activity; gamma DNA-directed DNA polymerase activity; iota DNA polymerase activity; kappa DNA polymerase activity; lambda DNA polymerase activity; mu DNA polymerase activity; nu DNA polymerase activity; sigma DNA polymerase activity; theta DNA polymerase activity; zeta DNA polymerase activity

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term DNA-directed DNA polymerase activity (63 genes) or to its variants containing one or more qualifiers (7 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	contributes_to: DNA-directed DNA polymerase activity	7
	DNA-directed DNA polymerase activity	58
High-throughput	none	none
Computational (download data)	DNA-directed DNA polymerase activity	60

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term DNA-directed DNA polymerase activity or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

contributes_to: DNA-directed DNA polymerase activity      7 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
DPB2/YPR175W	IDA: Inferred from Direct Assay Assigned on 2012-09-26	manually curated	Asturias FJ, et al.  (2006) Structure of Saccharomyces cerevisiae DNA polymerase epsilon by cryo-electron microscopy. Nat Struct Mol Biol 13(1):35-43	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-09-21	manually curated	Shcherbakova PV, et al.  (2003) Unique error signature of the four-subunit yeast DNA polymerase epsilon. J Biol Chem 278(44):43770-80	SGD
DPB3/YBR278W	IMP: Inferred from Mutant Phenotype Assigned on 2012-09-21	manually curated	Araki H, et al.  (1991) Cloning DPB3, the gene encoding the third subunit of DNA polymerase II of Saccharomyces cerevisiae. Nucleic Acids Res 19(18):4867-72	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-09-26	manually curated	Asturias FJ, et al.  (2006) Structure of Saccharomyces cerevisiae DNA polymerase epsilon by cryo-electron microscopy. Nat Struct Mol Biol 13(1):35-43	SGD
DPB4/YDR121W	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction with SGD:POL2 Assigned on 2007-09-20	manually curated	Ohya T, et al.  (2000) Structure and function of the fourth subunit (Dpb4p) of DNA polymerase epsilon in Saccharomyces cerevisiae. Nucleic Acids Res 28(20):3846-52	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-09-26	manually curated	Asturias FJ, et al.  (2006) Structure of Saccharomyces cerevisiae DNA polymerase epsilon by cryo-electron microscopy. Nat Struct Mol Biol 13(1):35-43	SGD
POL12/YBL035C	IDA: Inferred from Direct Assay Assigned on 2006-09-13	manually curated	Plevani P, et al.  (1985) Polypeptide structure of DNA primase from a yeast DNA polymerase-primase complex. J Biol Chem 260(11):7102-7	SGD
	IDA: Inferred from Direct Assay Assigned on 2006-09-13	manually curated	Brooke RG, et al.  (1991) Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities. J Biol Chem 266(5):3005-15	SGD
POL31/YJR006W	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Burgers PM  (1998) Eukaryotic DNA polymerases in DNA replication and DNA repair. Chromosoma 107(4):218-27	SGD
POL32/YJR043C	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Burgers PM  (1998) Eukaryotic DNA polymerases in DNA replication and DNA repair. Chromosoma 107(4):218-27	SGD
REV7/YIL139C	IDA: Inferred from Direct Assay Assigned on 2010-02-02	manually curated	Nelson JR, et al.  (1996) Thymine-thymine dimer bypass by yeast DNA polymerase zeta. Science 272(5268):1646-9	SGD

DNA-directed DNA polymerase activity      63 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
YPR137C-B	ISS: Inferred from Sequence or structural Similarity Assigned on 2003-08-11	manually curated	Kim JM, et al.  (1998) Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence. Genome Res 8(5):464-78	SGD
	IEA: Inferred from Electronic Annotation with IUBMB:2.7.7.7 Last Updated 2013-03-02	computational	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0239 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
YPR158C-D	ISS: Inferred from Sequence or structural Similarity Assigned on 2003-08-11	manually curated	Kim JM, et al.  (1998) Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence. Genome Res 8(5):464-78	SGD
	IEA: Inferred from Electronic Annotation with IUBMB:2.7.7.7 Last Updated 2013-03-02	computational	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0239 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
YPR158W-B	ISS: Inferred from Sequence or structural Similarity Assigned on 2003-08-11	manually curated	Kim JM, et al.  (1998) Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence. Genome Res 8(5):464-78	SGD
	IEA: Inferred from Electronic Annotation with IUBMB:2.7.7.7 Last Updated 2013-03-02	computational	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0239 Last Updated 2013-03-02	computational	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

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