Ontology: Molecular Function (GO:0000386)

Definition: Catalysis of the second transesterification reaction of spliceosomal mRNA splicing. Ligation of the two exons occurs via a transesterification reaction where the free 3'-hydroxyl group of the 5' exon is the nucleophile attacking the 3' splice site. Non-expressed sequences are now detached from the exons. In cis splicing, the intron is in a lariat structure.

Synonyms: lariat formation, 5'-splice site cleavage; 3'-splice site cleavage, exon ligation

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term second spliceosomal transesterification activity (0 genes) or to its variants containing one or more qualifiers (15 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	contributes_to: second spliceosomal transesterification activity	15
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term second spliceosomal transesterification activity or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

contributes_to: second spliceosomal transesterification activity      15 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
CDC40/YDR364C	IGI: Inferred from Genetic Interaction, IMP: Inferred from Mutant Phenotype Assigned on 2009-10-30	manually curated	Lindsey-Boltz LA, et al.  (2000) The carboxy terminal WD domain of the pre-mRNA splicing factor Prp17p is critical for function. RNA 6(9):1289-305	SGD
CEF1/YMR213W	IMP: Inferred from Mutant Phenotype Assigned on 2013-03-06	manually curated	Query CC and Konarska MM  (2012) CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome. RNA 18(5):1001-13	SGD
CLF1/YLR117C	IC: Inferred By Curator from U2-type catalytic step 2 spliceosome Assigned on 2009-10-20	manually curated	Russell CS, et al.  (2000) Functional analyses of interacting factors involved in both pre-mRNA splicing and cell cycle progression in Saccharomyces cerevisiae. RNA 6(11):1565-72	SGD
CWC2/YDL209C	IC: Inferred By Curator from U2-type catalytic step 2 spliceosome Assigned on 2009-10-22	manually curated	McGrail JC, et al.  (2009) The RNA binding protein Cwc2 interacts directly with the U6 snRNA to link the nineteen complex to the spliceosome during pre-mRNA splicing. Nucleic Acids Res 37(13):4205-17	SGD
ISY1/YJR050W	IC: Inferred By Curator from U2-type catalytic step 2 spliceosome Assigned on 2009-10-22	manually curated	Dix I, et al.  (1999) The identification and characterization of a novel splicing protein, Isy1p, of Saccharomyces cerevisiae. RNA 5(3):360-8	SGD
PRP16/YKR086W	IMP: Inferred from Mutant Phenotype Assigned on 2009-10-05	manually curated	Schwer B and Guthrie C  (1991) PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome. Nature 349(6309):494-9	SGD
PRP18/YGR006W	IDA: Inferred from Direct Assay Assigned on 2005-09-21	manually curated	James SA, et al.  (2002) How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing. RNA 8(8):1068-77	SGD
	IGI: Inferred from Genetic Interaction with SGD:PRP8 Assigned on 2008-04-11	manually curated	Aronova A, et al.  (2007) Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing. RNA 13(9):1437-44	SGD
PRP22/YER013W	IMP: Inferred from Mutant Phenotype Assigned on 2009-10-23	manually curated	Schwer B and Gross CH  (1998) Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing. EMBO J 17(7):2086-94	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-09-21	manually curated	James SA, et al.  (2002) How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing. RNA 8(8):1068-77	SGD
PRP45/YAL032C	IC: Inferred By Curator from U2-type catalytic step 2 spliceosome Assigned on 2009-10-21	manually curated	Albers M, et al.  (2003) Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors. RNA 9(1):138-50	SGD
PRP8/YHR165C	IMP: Inferred from Mutant Phenotype Assigned on 2008-04-11	manually curated	Aronova A, et al.  (2007) Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing. RNA 13(9):1437-44	SGD
SLU7/YDR088C	IDA: Inferred from Direct Assay Assigned on 2005-09-21	manually curated	James SA, et al.  (2002) How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing. RNA 8(8):1068-77	SGD
SNR7-L/snR7-L	IMP: Inferred from Mutant Phenotype Assigned on 2008-04-08	manually curated	O'Keefe RT and Newman AJ  (1998) Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing. EMBO J 17(2):565-74	SGD
SNR7-S/snR7-S	IMP: Inferred from Mutant Phenotype Assigned on 2008-04-08	manually curated	O'Keefe RT and Newman AJ  (1998) Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing. EMBO J 17(2):565-74	SGD
SYF1/YDR416W	IC: Inferred By Curator from U2-type catalytic step 2 spliceosome Assigned on 2009-10-20	manually curated	Russell CS, et al.  (2000) Functional analyses of interacting factors involved in both pre-mRNA splicing and cell cycle progression in Saccharomyces cerevisiae. RNA 6(11):1565-72	SGD
SYF2/YGR129W	IC: Inferred By Curator from U2-type catalytic step 2 spliceosome Assigned on 2009-10-20	manually curated	Russell CS, et al.  (2000) Functional analyses of interacting factors involved in both pre-mRNA splicing and cell cycle progression in Saccharomyces cerevisiae. RNA 6(11):1565-72	SGD

SGD Home About Blog Site Map Help Terms of Use
© Stanford University, Stanford, CA 94305.