Ontology: Molecular Function (GO:0003697)

Definition: Interacting selectively and non-covalently with single-stranded DNA.

Synonyms: ssDNA binding

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term single-stranded DNA binding (29 genes) or to its variants containing one or more qualifiers (13 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	contributes_to: single-stranded DNA binding	13
	single-stranded DNA binding	27
High-throughput	none	none
Computational (download data)	single-stranded DNA binding	3

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term single-stranded DNA binding or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

single-stranded DNA binding      29 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
ACO1/YLR304C	IDA: Inferred from Direct Assay Assigned on 2007-08-21	manually curated	Chen XJ, et al.  (2007) Yeast aconitase binds and provides metabolically coupled protection to mitochondrial DNA. Proc Natl Acad Sci U S A 104(34):13738-43	SGD
ASM4/YDL088C	IDA: Inferred from Direct Assay Assigned on 2011-05-25	manually curated	Patel SS and Rexach MF  (2008) Discovering novel interactions at the nuclear pore complex using bead halo: a rapid method for detecting molecular interactions of high and low affinity at equilibrium. Mol Cell Proteomics 7(1):121-31	SGD
DMC1/YER179W	IDA: Inferred from Direct Assay Assigned on 2002-05-21	manually curated	Hong EL, et al.  (2001) Saccharomyces cerevisiae Dmc1 protein promotes renaturation of single-strand DNA (ssDNA) and assimilation of ssDNA into homologous super-coiled duplex DNA. J Biol Chem 276(45):41906-12	SGD
EST1/YLR233C	IDA: Inferred from Direct Assay Assigned on 2007-07-23	manually curated	Virta-Pearlman V, et al.  (1996) Est1 has the properties of a single-stranded telomere end-binding protein. Genes Dev 10(24):3094-104	SGD
FLP1/R0010W	IDA: Inferred from Direct Assay Assigned on 2006-07-21	manually curated	Zhu XD and Sadowski PD  (1998) Selection of novel, specific single-stranded DNA sequences by Flp, a duplex-specific DNA binding protein. Nucleic Acids Res 26(5):1329-36	SGD
GIS2/YNL255C	IDA: Inferred from Direct Assay Assigned on 2011-02-04	manually curated	Scherrer T, et al.  (2011) Defining potentially conserved RNA regulons of homologous zinc-finger RNA-binding proteins. Genome Biol 12(1):R3	SGD
HSP60/YLR259C	IDA: Inferred from Direct Assay Assigned on 2004-07-16	manually curated	Kaufman BA, et al.  (2000) In organello formaldehyde crosslinking of proteins to mtDNA: identification of bifunctional proteins. Proc Natl Acad Sci U S A 97(14):7772-7	SGD
ISW2/YOR304W	IDA: Inferred from Direct Assay Assigned on 2009-02-17	manually curated	Fischer CJ, et al.  (2009) Kinetic mechanism for single-stranded DNA binding and translocation by Saccharomyces cerevisiae Isw2. Biochemistry 48(13):2960-8	SGD
LAP3/YNL239W	IDA: Inferred from Direct Assay Assigned on 2011-06-23	manually curated	Zheng W and Johnston SA  (1998) The nucleic acid binding activity of bleomycin hydrolase is involved in bleomycin detoxification. Mol Cell Biol 18(6):3580-5	SGD
MCM10/YIL150C	IDA: Inferred from Direct Assay Assigned on 2009-07-22	manually curated	Eisenberg S, et al.  (2009) Novel DNA Binding Properties of the Mcm10 Protein from Saccharomyces cerevisiae. J Biol Chem 284(37):25412-20	SGD
MCM4/YPR019W	IMP: Inferred from Mutant Phenotype Assigned on 2008-01-31	manually curated	Bochman ML and Schwacha A  (2007) Differences in the single-stranded DNA binding activities of MCM2-7 and MCM467: MCM2 and MCM5 define a slow ATP-dependent step. J Biol Chem 282(46):33795-804	SGD
MHR1/YDR296W	IDA: Inferred from Direct Assay Assigned on 2009-09-16	manually curated	Masuda T, et al.  (2009) A non-canonical DNA structure enables homologous recombination in various genetic systems. J Biol Chem 284(44):30230-9	SGD
NSR1/YGR159C	IDA: Inferred from Direct Assay Assigned on 2002-12-05	manually curated	Lin JJ and Zakian VA  (1994) Isolation and characterization of two Saccharomyces cerevisiae genes that encode proteins that bind to (TG1-3)n single strand telomeric DNA in vitro. Nucleic Acids Res 22(23):4906-13	SGD
NUP53/YMR153W	IDA: Inferred from Direct Assay Assigned on 2011-05-25	manually curated	Patel SS and Rexach MF  (2008) Discovering novel interactions at the nuclear pore complex using bead halo: a rapid method for detecting molecular interactions of high and low affinity at equilibrium. Mol Cell Proteomics 7(1):121-31	SGD
PIM1/YBL022C	IBA: Inferred from Biological Aspect of Ancestor with PANTHER:PTHR10046_AN2 Assigned on 2010-04-12	computational	Gaudet P, et al.  (2011) Annotation inferences using phylogenetic trees.	RefGenome
POL2/YNL262W	IDA: Inferred from Direct Assay Assigned on 2012-09-21	manually curated	Tsubota T, et al.  (2003) Double-stranded DNA binding properties of Saccharomyces cerevisiae DNA polymerase epsilon and of the Dpb3p-Dpb4p subassembly. Genes Cells 8(11):873-88	SGD
RAD1/YPL022W	IDA: Inferred from Direct Assay Assigned on 2007-10-11	manually curated	Sung P, et al.  (1993) Purification and characterization of the Saccharomyces cerevisiae RAD1/RAD10 endonuclease. J Biol Chem 268(35):26391-9	SGD
RAD10/YML095C	IDA: Inferred from Direct Assay Assigned on 2006-03-08	manually curated	Sung P, et al.  (1992) Renaturation of DNA catalysed by yeast DNA repair and recombination protein RAD10. Nature 355(6362):743-5	SGD
RAD18/YCR066W	IDA: Inferred from Direct Assay Assigned on 2009-11-11	manually curated	Bailly V, et al.  (1994) Specific complex formation between yeast RAD6 and RAD18 proteins: a potential mechanism for targeting RAD6 ubiquitin-conjugating activity to DNA damage sites. Genes Dev 8(7):811-20	SGD
RAD2/YGR258C	IEA: Inferred from Electronic Annotation with EBI:IPR001044 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
RAD51/YER095W	IDA: Inferred from Direct Assay Assigned on 2010-04-13	manually curated	Chen J, et al.  (2010) Insights into the mechanism of Rad51 recombinase from the structure and properties of a filament interface mutant. Nucleic Acids Res 38(14):4889-906	SGD
RFA1/YAR007C	IDA: Inferred from Direct Assay Assigned on 2007-09-18	manually curated	Philipova D, et al.  (1996) A hierarchy of SSB protomers in replication protein A. Genes Dev 10(17):2222-33	SGD
RFA2/YNL312W	IDA: Inferred from Direct Assay Assigned on 2007-09-18	manually curated	Philipova D, et al.  (1996) A hierarchy of SSB protomers in replication protein A. Genes Dev 10(17):2222-33	SGD
RIM1/YCR028C-A	IDA: Inferred from Direct Assay Assigned on 2007-11-12	manually curated	Van Dyck E, et al.  (1992) A single-stranded DNA binding protein required for mitochondrial DNA replication in S. cerevisiae is homologous to E. coli SSB. EMBO J 11(9):3421-30	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR011344, EBI:IPR000424 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
RMI1/YPL024W	IDA: Inferred from Direct Assay Assigned on 2006-07-26	manually curated	Mullen JR, et al.  (2005) Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex. Mol Cell Biol 25(11):4476-87	SGD
RPB7/YDR404C	IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2010-01-19	manually curated	Orlicky SM, et al.  (2001) Dissociable Rpb4-Rpb7 subassembly of rna polymerase II binds to single-strand nucleic acid and mediates a post-recruitment step in transcription initiation. J Biol Chem 276(13):10097-102	SGD
SIR3/YLR442C	IDA: Inferred from Direct Assay Assigned on 2009-03-11	manually curated	Adkins NL, et al.  (2009) Role of nucleic acid binding in Sir3p-dependent interactions with chromatin fibers. Biochemistry 48(2):276-88	SGD
SLD2/YKL108W	IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2011-07-19	manually curated	Kanter DM and Kaplan DL  (2011) Sld2 binds to origin single-stranded DNA and stimulates DNA annealing. Nucleic Acids Res 39(7):2580-92	SGD
SMC5/YOL034W	IDA: Inferred from Direct Assay Assigned on 2011-09-21	manually curated	Roy MA, et al.  (2011) Dynamic and selective DNA-binding activity of Smc5, a core component of the Smc5-Smc6 complex. Cell Cycle 10(4):690-700	SGD

contributes_to: single-stranded DNA binding      13 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
DPB2/YPR175W	IDA: Inferred from Direct Assay Assigned on 2012-09-21	manually curated	Tsubota T, et al.  (2003) Double-stranded DNA binding properties of Saccharomyces cerevisiae DNA polymerase epsilon and of the Dpb3p-Dpb4p subassembly. Genes Cells 8(11):873-88	SGD
DPB3/YBR278W	IDA: Inferred from Direct Assay Assigned on 2012-09-21	manually curated	Tsubota T, et al.  (2003) Double-stranded DNA binding properties of Saccharomyces cerevisiae DNA polymerase epsilon and of the Dpb3p-Dpb4p subassembly. Genes Cells 8(11):873-88	SGD
DPB4/YDR121W	IDA: Inferred from Direct Assay Assigned on 2012-09-21	manually curated	Tsubota T, et al.  (2003) Double-stranded DNA binding properties of Saccharomyces cerevisiae DNA polymerase epsilon and of the Dpb3p-Dpb4p subassembly. Genes Cells 8(11):873-88	SGD
MCM2/YBL023C	IMP: Inferred from Mutant Phenotype Assigned on 2008-01-31	manually curated	Bochman ML and Schwacha A  (2007) Differences in the single-stranded DNA binding activities of MCM2-7 and MCM467: MCM2 and MCM5 define a slow ATP-dependent step. J Biol Chem 282(46):33795-804	SGD
MCM5/YLR274W	IMP: Inferred from Mutant Phenotype Assigned on 2008-01-31	manually curated	Bochman ML and Schwacha A  (2007) Differences in the single-stranded DNA binding activities of MCM2-7 and MCM467: MCM2 and MCM5 define a slow ATP-dependent step. J Biol Chem 282(46):33795-804	SGD
MCM7/YBR202W	IMP: Inferred from Mutant Phenotype Assigned on 2008-01-31	manually curated	Bochman ML and Schwacha A  (2007) Differences in the single-stranded DNA binding activities of MCM2-7 and MCM467: MCM2 and MCM5 define a slow ATP-dependent step. J Biol Chem 282(46):33795-804	SGD
MLH1/YMR167W	IDA: Inferred from Direct Assay Assigned on 2007-04-06	manually curated	Hall MC, et al.  (2001) High affinity cooperative DNA binding by the yeast Mlh1-Pms1 heterodimer. J Mol Biol 312(4):637-47	SGD
PMS1/YNL082W	IDA: Inferred from Direct Assay Assigned on 2007-04-06	manually curated	Hall MC, et al.  (2001) High affinity cooperative DNA binding by the yeast Mlh1-Pms1 heterodimer. J Mol Biol 312(4):637-47	SGD
PRI1/YIR008C	IDA: Inferred from Direct Assay Assigned on 2010-05-03	manually curated	Sauguet L, et al.  (2010) Shared active site architecture between the large subunit of eukaryotic primase and DNA photolyase. PLoS One 5(4):e10083	SGD
PRI2/YKL045W	IDA: Inferred from Direct Assay Assigned on 2010-05-03	manually curated	Sauguet L, et al.  (2010) Shared active site architecture between the large subunit of eukaryotic primase and DNA photolyase. PLoS One 5(4):e10083	SGD
RPB4/YJL140W	IDA: Inferred from Direct Assay Assigned on 2010-01-15	manually curated	Orlicky SM, et al.  (2001) Dissociable Rpb4-Rpb7 subassembly of rna polymerase II binds to single-strand nucleic acid and mediates a post-recruitment step in transcription initiation. J Biol Chem 276(13):10097-102	SGD
TFA1/YKL028W	IDA: Inferred from Direct Assay Assigned on 2012-01-13	manually curated	Kuldell NH and Buratowski S  (1997) Genetic analysis of the large subunit of yeast transcription factor IIE reveals two regions with distinct functions. Mol Cell Biol 17(9):5288-98	SGD
TFA2/YKR062W	IDA: Inferred from Direct Assay Assigned on 2012-01-13	manually curated	Kuldell NH and Buratowski S  (1997) Genetic analysis of the large subunit of yeast transcription factor IIE reveals two regions with distinct functions. Mol Cell Biol 17(9):5288-98	SGD

SGD Home About Blog Site Map Help Terms of Use
© Stanford University, Stanford, CA 94305.