Ontology: Molecular Function (GO:0003688)

Definition: Interacting selectively and non-covalently with the DNA replication origin, a unique DNA sequence of a replicon at which DNA replication is initiated and proceeds bidirectionally or unidirectionally.

Synonyms: ARS binding

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term DNA replication origin binding (30 genes) or to its variants containing one or more qualifiers (1 gene). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	DNA replication origin binding	30
	NOT: DNA replication origin binding	1
High-throughput	none	none
Computational (download data)	DNA replication origin binding	1

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term DNA replication origin binding or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

DNA replication origin binding      30 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
ABF1/YKL112W	IDA: Inferred from Direct Assay Assigned on 2012-02-15	manually curated	Diffley JF and Stillman B  (1988) Purification of a yeast protein that binds to origins of DNA replication and a transcriptional silencer. Proc Natl Acad Sci U S A 85(7):2120-4	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-24	manually curated	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD
ADE12/YNL220W	IDA: Inferred from Direct Assay Assigned on 2007-03-27	manually curated	Gallert KC, et al.  (1996) Enzymatic properties and inhibition by single-stranded autonomously replicating sequences of adenylosuccinate synthase from Saccharomyces cerevisiae. Eur J Biochem 239(2):487-93	SGD
BMH1/YER177W	IDA: Inferred from Direct Assay Assigned on 2007-08-20	manually curated	Callejo M, et al.  (2002) The 14-3-3 protein homologues from Saccharomyces cerevisiae, Bmh1p and Bmh2p, have cruciform DNA-binding activity and associate in vivo with ARS307. J Biol Chem 277(41):38416-23	SGD
BMH2/YDR099W	IDA: Inferred from Direct Assay Assigned on 2007-08-20	manually curated	Callejo M, et al.  (2002) The 14-3-3 protein homologues from Saccharomyces cerevisiae, Bmh1p and Bmh2p, have cruciform DNA-binding activity and associate in vivo with ARS307. J Biol Chem 277(41):38416-23	SGD
CDC45/YLR103C	IDA: Inferred from Direct Assay Assigned on 2007-08-14	manually curated	Sclafani RA, et al.  (2002) The mcm5-bob1 bypass of Cdc7p/Dbf4p in DNA replication depends on both Cdk1-independent and Cdk1-dependent steps in Saccharomyces cerevisiae. Genetics 161(1):47-57	SGD
CDC53/YDL132W	IPI: Inferred from Physical Interaction Assigned on 2006-01-23	manually curated	Koepp DM, et al.  (2006) The F-box protein Dia2 regulates DNA replication. Mol Biol Cell 17(4):1540-8	SGD
CDC6/YJL194W	IDA: Inferred from Direct Assay Assigned on 2007-08-09	manually curated	Tanaka T, et al.  (1997) Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs. Cell 90(4):649-60	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-24	manually curated	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD
CLF1/YLR117C	IDA: Inferred from Direct Assay Assigned on 2010-04-21	manually curated	Zhu W, et al.  (2002) Evidence that the pre-mRNA splicing factor Clf1p plays a role in DNA replication in Saccharomyces cerevisiae. Genetics 160(4):1319-33	SGD
DBF4/YDR052C	IDA: Inferred from Direct Assay Assigned on 2006-12-05	manually curated	Dowell SJ, et al.  (1994) Interaction of Dbf4, the Cdc7 protein kinase regulatory subunit, with yeast replication origins in vivo. Science 265(5176):1243-6	SGD
DIA2/YOR080W	IPI: Inferred from Physical Interaction Assigned on 2006-01-23	manually curated	Koepp DM, et al.  (2006) The F-box protein Dia2 regulates DNA replication. Mol Biol Cell 17(4):1540-8	SGD
HSP60/YLR259C	IDA: Inferred from Direct Assay Assigned on 2008-07-18	manually curated	Kaufman BA, et al.  (2000) In organello formaldehyde crosslinking of proteins to mtDNA: identification of bifunctional proteins. Proc Natl Acad Sci U S A 97(14):7772-7	SGD
MCM1/YMR043W	IDA: Inferred from Direct Assay Assigned on 2003-03-04	manually curated	Chang VK, et al.  (2003) Mcm1 binds replication origins. J Biol Chem 278(8):6093-100	SGD
MCM10/YIL150C	IDA: Inferred from Direct Assay Assigned on 2007-08-13	manually curated	Homesley L, et al.  (2000) Mcm10 and the MCM2-7 complex interact to initiate DNA synthesis and to release replication factors from origins. Genes Dev 14(8):913-26	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-15	manually curated	Ricke RM and Bielinsky AK  (2004) Mcm10 regulates the stability and chromatin association of DNA polymerase-alpha. Mol Cell 16(2):173-85	SGD
MCM2/YBL023C	IDA: Inferred from Direct Assay Assigned on 2007-08-14	manually curated	Wilmes GM and Bell SP  (2002) The B2 element of the Saccharomyces cerevisiae ARS1 origin of replication requires specific sequences to facilitate pre-RC formation. Proc Natl Acad Sci U S A 99(1):101-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-24	manually curated	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD
MCM3/YEL032W	IDA: Inferred from Direct Assay Assigned on 2007-08-14	manually curated	Wilmes GM and Bell SP  (2002) The B2 element of the Saccharomyces cerevisiae ARS1 origin of replication requires specific sequences to facilitate pre-RC formation. Proc Natl Acad Sci U S A 99(1):101-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-24	manually curated	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD
MCM4/YPR019W	IDA: Inferred from Direct Assay Assigned on 2007-08-14	manually curated	Wilmes GM and Bell SP  (2002) The B2 element of the Saccharomyces cerevisiae ARS1 origin of replication requires specific sequences to facilitate pre-RC formation. Proc Natl Acad Sci U S A 99(1):101-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-24	manually curated	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD
MCM5/YLR274W	IDA: Inferred from Direct Assay Assigned on 2007-08-14	manually curated	Wilmes GM and Bell SP  (2002) The B2 element of the Saccharomyces cerevisiae ARS1 origin of replication requires specific sequences to facilitate pre-RC formation. Proc Natl Acad Sci U S A 99(1):101-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-24	manually curated	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD
MCM6/YGL201C	IDA: Inferred from Direct Assay Assigned on 2007-08-14	manually curated	Wilmes GM and Bell SP  (2002) The B2 element of the Saccharomyces cerevisiae ARS1 origin of replication requires specific sequences to facilitate pre-RC formation. Proc Natl Acad Sci U S A 99(1):101-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-24	manually curated	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD
MCM7/YBR202W	IDA: Inferred from Direct Assay Assigned on 2007-08-09	manually curated	Tanaka T, et al.  (1997) Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs. Cell 90(4):649-60	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-14	manually curated	Wilmes GM and Bell SP  (2002) The B2 element of the Saccharomyces cerevisiae ARS1 origin of replication requires specific sequences to facilitate pre-RC formation. Proc Natl Acad Sci U S A 99(1):101-6	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-24	manually curated	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD
ORC1/YML065W	IDA: Inferred from Direct Assay Assigned on 2007-08-24	manually curated	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD
ORC2/YBR060C	IDA: Inferred from Direct Assay Assigned on 2007-08-09	manually curated	Tanaka T, et al.  (1997) Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs. Cell 90(4):649-60	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-08-24	manually curated	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD
ORC3/YLL004W	IDA: Inferred from Direct Assay Assigned on 2007-08-24	manually curated	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD
ORC4/YPR162C	IDA: Inferred from Direct Assay Assigned on 2007-08-24	manually curated	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD
ORC5/YNL261W	IDA: Inferred from Direct Assay Assigned on 2007-08-24	manually curated	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD
ORC6/YHR118C	IDA: Inferred from Direct Assay Assigned on 2007-08-24	manually curated	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD
RAD53/YPL153C	IDA: Inferred from Direct Assay Assigned on 2006-08-16	manually curated	Dohrmann PR and Sclafani RA  (2006) Novel role for checkpoint Rad53 protein kinase in the initiation of chromosomal DNA replication in Saccharomyces cerevisiae. Genetics 174(1):87-99	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR016256 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
SKP1/YDR328C	IPI: Inferred from Physical Interaction Assigned on 2006-01-23	manually curated	Koepp DM, et al.  (2006) The F-box protein Dia2 regulates DNA replication. Mol Biol Cell 17(4):1540-8	SGD
SLD2/YKL108W	IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2011-07-19	manually curated	Kanter DM and Kaplan DL  (2011) Sld2 binds to origin single-stranded DNA and stimulates DNA annealing. Nucleic Acids Res 39(7):2580-92	SGD
SUM1/YDR310C	IDA: Inferred from Direct Assay Assigned on 2008-06-10	manually curated	Irlbacher H, et al.  (2005) Control of replication initiation and heterochromatin formation in Saccharomyces cerevisiae by a regulator of meiotic gene expression. Genes Dev 19(15):1811-22	SGD
TAH11/YJR046W	IDA: Inferred from Direct Assay Assigned on 2007-08-24	manually curated	Kawasaki Y, et al.  (2006) Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells 11(7):745-56	SGD

NOT: DNA replication origin binding      1 gene directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
TAH11/YJR046W	IDA: Inferred from Direct Assay Assigned on 2007-09-15	manually curated	Asano T, et al.  (2007) Interaction between ORC and Cdt1p of Saccharomyces cerevisiae. FEMS Yeast Res 7(8):1256-62	SGD

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