Ontology: Biological Process (GO:0000350)

Definition: Conformational rearrangement of the spliceosomal complex containing the RNA products from the 1st step of splicing to form the catalytic site for the second step of splicing.

Synonyms: catalytic spliceosome assembly for second transesterification step; formation of catalytic spliceosome for second transesterification step; formation of spliceosomal A2-2 complex; formation of spliceosomal C1 complex; lariat formation, 5'-splice site cleavage; spliceosomal A2-3 complex biosynthesis; spliceosomal A2-3 complex formation; spliceosomal C2 complex biosynthesis; spliceosomal C2 complex formation; formation of catalytic U12-type spliceosome for second transesterification step; formation of catalytic U2-type spliceosome for second transesterification step

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term generation of catalytic spliceosome for second transesterification step (12 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	generation of catalytic spliceosome for second transesterification step	12
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term generation of catalytic spliceosome for second transesterification step or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

generation of catalytic spliceosome for second transesterification step      12 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
CDC40/YDR364C	IGI: Inferred from Genetic Interaction, IMP: Inferred from Mutant Phenotype Assigned on 2009-10-30	manually curated	Lindsey-Boltz LA, et al.  (2000) The carboxy terminal WD domain of the pre-mRNA splicing factor Prp17p is critical for function. RNA 6(9):1289-305	SGD
CEF1/YMR213W	IMP: Inferred from Mutant Phenotype Assigned on 2013-03-06	manually curated	Query CC and Konarska MM  (2012) CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome. RNA 18(5):1001-13	SGD
ISY1/YJR050W	IMP: Inferred from Mutant Phenotype Assigned on 2009-10-19	manually curated	Dix I, et al.  (1999) The identification and characterization of a novel splicing protein, Isy1p, of Saccharomyces cerevisiae. RNA 5(3):360-8	SGD
PRP16/YKR086W	IMP: Inferred from Mutant Phenotype Assigned on 2009-06-19	manually curated	Villa T and Guthrie C  (2005) The Isy1p component of the NineTeen complex interacts with the ATPase Prp16p to regulate the fidelity of pre-mRNA splicing. Genes Dev 19(16):1894-904	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-10-05	manually curated	Schwer B and Guthrie C  (1991) PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome. Nature 349(6309):494-9	SGD
PRP18/YGR006W	IDA: Inferred from Direct Assay Assigned on 2005-09-21	manually curated	James SA, et al.  (2002) How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing. RNA 8(8):1068-77	SGD
PRP22/YER013W	IDA: Inferred from Direct Assay Assigned on 2005-09-21	manually curated	James SA, et al.  (2002) How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing. RNA 8(8):1068-77	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2013-03-06	manually curated	Query CC and Konarska MM  (2012) CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome. RNA 18(5):1001-13	SGD
PRP45/YAL032C	IGI: Inferred from Genetic Interaction with SGD:SLU7, SGD:CDC40, SGD:PRP18, SGD:PRP22, SGD:SYF1, SGD:CLF1, SGD:NTC20, SGD:CEF1, IMP: Inferred from Mutant Phenotype Assigned on 2009-09-22	manually curated	Gahura O, et al.  (2009) Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates. J Cell Biochem 106(1):139-51	SGD
PRP8/YHR165C	IMP: Inferred from Mutant Phenotype Assigned on 2013-03-06	manually curated	Query CC and Konarska MM  (2012) CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome. RNA 18(5):1001-13	SGD
SLU7/YDR088C	IDA: Inferred from Direct Assay Assigned on 2005-09-21	manually curated	James SA, et al.  (2002) How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing. RNA 8(8):1068-77	SGD
SNR6/snR6	IMP: Inferred from Mutant Phenotype Assigned on 2013-03-06	manually curated	Query CC and Konarska MM  (2012) CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome. RNA 18(5):1001-13	SGD
SNR7-L/snR7-L	IMP: Inferred from Mutant Phenotype Assigned on 2008-04-08	manually curated	O'Keefe RT and Newman AJ  (1998) Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing. EMBO J 17(2):565-74	SGD
SNR7-S/snR7-S	IMP: Inferred from Mutant Phenotype Assigned on 2008-04-08	manually curated	O'Keefe RT and Newman AJ  (1998) Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing. EMBO J 17(2):565-74	SGD

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