GO term: DNA replication preinitiation complex 
Ontology: Cellular Component (GO:0031261)
Definition: A protein-DNA complex assembled at eukaryotic DNA replication origins immediately prior to the initiation of DNA replication. The preinitiation complex is formed by the assembly of additional proteins onto an existing prereplicative complex. In budding yeast, the additional proteins include Cdc45p, Sld2p, Sld3p, Dpb11p, DNA polymerases, and others; in fission yeast the GINS complex is present.
Synonyms: pre-IC
View Ontology:
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(text)
Definition: A protein-DNA complex assembled at eukaryotic DNA replication origins immediately prior to the initiation of DNA replication. The preinitiation complex is formed by the assembly of additional proteins onto an existing prereplicative complex. In budding yeast, the additional proteins include Cdc45p, Sld2p, Sld3p, Dpb11p, DNA polymerases, and others; in fission yeast the GINS complex is present.
Synonyms: pre-IC
View Ontology:
(graph) | (text)
This table lists the methods used to annotate genes either directly to the term
DNA replication preinitiation complex (22 genes)
or to its variants containing one or more
qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.
Links to Additional Annotations:
| Annotation Method | GO Term | # Yeast Genes Annotated |
|---|---|---|
| Manually curated (download data) | DNA replication preinitiation complex | 22 |
| High-throughput | none | none |
| Computational | none | none |
Links to Additional Annotations:
- View
annotations in multiple organisms using
- Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.
Annotation details for genes that have been directly annotated to the term
DNA replication preinitiation complex or its variants containing one or more
qualifiers (NOT, contributes to, or colocalizes with).
| DNA replication preinitiation complex 22 genes directly annotated to this term |
||||
|---|---|---|---|---|
| Locus | Evidence | Annotation Method | Reference | Assigned By |
| CDC45/YLR103C | IDA: Inferred from Direct Assay Assigned on 2007-08-02 |
manually curated | Zou L and Stillman B (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6 |
SGD |
| CDC6/YJL194W | IDA: Inferred from Direct Assay Assigned on 2007-08-02 |
manually curated | Zou L and Stillman B (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6 |
SGD |
| DPB11/YJL090C | IMP: Inferred from Mutant Phenotype Assigned on 2007-08-02 |
manually curated | Tanaka S, et al. (2007) CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast. Nature 445(7125):328-32 |
SGD |
| MCM2/YBL023C | IDA: Inferred from Direct Assay Assigned on 2007-08-02 |
manually curated | Zou L and Stillman B (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6 |
SGD |
| MCM3/YEL032W | IPI: Inferred from Physical Interaction with SGD:CDC45 Assigned on 2007-08-02 |
manually curated | Zou L and Stillman B (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6 |
SGD |
| MCM4/YPR019W | IDA: Inferred from Direct Assay Assigned on 2007-08-02 |
manually curated | Zou L and Stillman B (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6 |
SGD |
| MCM5/YLR274W | IPI: Inferred from Physical Interaction with SGD:CDC45 Assigned on 2007-08-02 |
manually curated | Zou L and Stillman B (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6 |
SGD |
| MCM6/YGL201C | IDA: Inferred from Direct Assay Assigned on 2007-08-02 |
manually curated | Zou L and Stillman B (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6 |
SGD |
| MCM7/YBR202W | IDA: Inferred from Direct Assay Assigned on 2007-08-02 |
manually curated | Zou L and Stillman B (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6 |
SGD |
| ORC1/YML065W | IDA: Inferred from Direct Assay Assigned on 2007-08-02 |
manually curated | Zou L and Stillman B (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6 |
SGD |
| ORC2/YBR060C | IDA: Inferred from Direct Assay Assigned on 2007-08-02 |
manually curated | Zou L and Stillman B (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6 |
SGD |
| ORC3/YLL004W | IDA: Inferred from Direct Assay Assigned on 2007-08-02 |
manually curated | Zou L and Stillman B (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6 |
SGD |
| ORC4/YPR162C | IDA: Inferred from Direct Assay Assigned on 2007-08-02 |
manually curated | Zou L and Stillman B (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6 |
SGD |
| ORC5/YNL261W | IDA: Inferred from Direct Assay Assigned on 2007-08-02 |
manually curated | Zou L and Stillman B (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6 |
SGD |
| ORC6/YHR118C | IDA: Inferred from Direct Assay Assigned on 2007-08-02 |
manually curated | Zou L and Stillman B (1998) Formation of a preinitiation complex by S-phase cyclin CDK-dependent loading of Cdc45p onto chromatin. Science 280(5363):593-6 |
SGD |
| PSF1/YDR013W | IGI: Inferred from Genetic Interaction with SGD:DPB11, IMP: Inferred from Mutant Phenotype Assigned on 2007-08-02 |
manually curated | Takayama Y, et al. (2003) GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes Dev 17(9):1153-65 |
SGD |
| PSF2/YJL072C | IPI: Inferred from Physical Interaction with SGD:PSF1 Assigned on 2007-08-02 |
manually curated | Takayama Y, et al. (2003) GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes Dev 17(9):1153-65 |
SGD |
| PSF3/YOL146W | IPI: Inferred from Physical Interaction with SGD:PSF1 Assigned on 2007-08-02 |
manually curated | Takayama Y, et al. (2003) GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes Dev 17(9):1153-65 |
SGD |
| SLD2/YKL108W | IDA: Inferred from Direct Assay, IGI: Inferred from Genetic Interaction with SGD:PSF1, SGD:PSF2, SGD:PSF3, SGD:SLD5 Assigned on 2011-07-19 |
manually curated | Muramatsu S, et al. (2010) CDK-dependent complex formation between replication proteins Dpb11, Sld2, Pol {varepsilon}, and GINS in budding yeast. Genes Dev 24(6):602-12 |
SGD |
| SLD3/YGL113W | IPI: Inferred from Physical Interaction with SGD:CDC45 Assigned on 2007-08-02 |
manually curated | Kamimura Y, et al. (2001) Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae. EMBO J 20(8):2097-107 |
SGD |
| SLD5/YDR489W | IGI: Inferred from Genetic Interaction with SGD:DPB11, IMP: Inferred from Mutant Phenotype Assigned on 2007-08-02 |
manually curated | Takayama Y, et al. (2003) GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes Dev 17(9):1153-65 |
SGD |
| SLD7/YOR060C | IDA: Inferred from Direct Assay Assigned on 2011-05-06 |
manually curated | Tanaka T, et al. (2011) Sld7, an Sld3-associated protein required for efficient chromosomal DNA replication in budding yeast. EMBO J 30(10):2019-30 |
SGD |
