Ontology: Molecular Function (GO:0030627)

Definition: Interacting selectively and non-covalently with the pre-mRNA 5' splice site sequence.

Synonyms: pre-mRNA 5' splice site binding

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term pre-mRNA 5'-splice site binding (5 genes) or to its variants containing one or more qualifiers (1 gene). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	contributes_to: pre-mRNA 5'-splice site binding	1
	pre-mRNA 5'-splice site binding	5
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term pre-mRNA 5'-splice site binding or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

pre-mRNA 5'-splice site binding      5 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
LSR1	IDA: Inferred from Direct Assay Assigned on 2008-04-02	manually curated	McGrail JC and O'Keefe RT  (2008) The U1, U2 and U5 snRNAs crosslink to the 5' exon during yeast pre-mRNA splicing. Nucleic Acids Res 36(3):814-25	SGD
RPL30/YGL030W	IGI: Inferred from Genetic Interaction with SGD:RPL30, IMP: Inferred from Mutant Phenotype Assigned on 2008-07-01	manually curated	Macias S, et al.  (2008) L30 binds the nascent RPL30 transcript to repress U2 snRNP recruitment. Mol Cell 30(6):732-42	SGD
SNR19/snR19	IGI: Inferred from Genetic Interaction, IMP: Inferred from Mutant Phenotype Assigned on 2005-10-27	manually curated	Seraphin B, et al.  (1988) A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5' cleavage site. EMBO J 7(8):2533-8	SGD
	IGI: Inferred from Genetic Interaction, IMP: Inferred from Mutant Phenotype Assigned on 2005-10-27	manually curated	Lacadie SA and Rosbash M  (2005) Cotranscriptional spliceosome assembly dynamics and the role of U1 snRNA:5'ss base pairing in yeast. Mol Cell 19(1):65-75	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-04-02	manually curated	McGrail JC and O'Keefe RT  (2008) The U1, U2 and U5 snRNAs crosslink to the 5' exon during yeast pre-mRNA splicing. Nucleic Acids Res 36(3):814-25	SGD
SNR7-L/snR7-L	IDA: Inferred from Direct Assay Assigned on 2002-07-17	manually curated	Alvi RK, et al.  (2001) ATP-dependent interaction of yeast U5 snRNA loop 1 with the 5' splice site. RNA 7(7):1013-23	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-04-08	manually curated	O'Keefe RT and Newman AJ  (1998) Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing. EMBO J 17(2):565-74	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-04-02	manually curated	McGrail JC and O'Keefe RT  (2008) The U1, U2 and U5 snRNAs crosslink to the 5' exon during yeast pre-mRNA splicing. Nucleic Acids Res 36(3):814-25	SGD
SNR7-S/snR7-S	IDA: Inferred from Direct Assay Assigned on 2008-04-08	manually curated	O'Keefe RT and Newman AJ  (1998) Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing. EMBO J 17(2):565-74	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-04-02	manually curated	McGrail JC and O'Keefe RT  (2008) The U1, U2 and U5 snRNAs crosslink to the 5' exon during yeast pre-mRNA splicing. Nucleic Acids Res 36(3):814-25	SGD

contributes_to: pre-mRNA 5'-splice site binding      1 gene directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
PRP39/YML046W	IGI: Inferred from Genetic Interaction with SGD:LSR1 Assigned on 2005-11-02	manually curated	Lockhart SR and Rymond BC  (1994) Commitment of yeast pre-mRNA to the splicing pathway requires a novel U1 small nuclear ribonucleoprotein polypeptide, Prp39p. Mol Cell Biol 14(6):3623-33	SGD

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