Ontology: Biological Process (GO:0030466)

Definition: Repression of transcription at silent mating-type loci by alteration of the structure of chromatin.

Synonyms: heterochromatic silencing at silent mating-type cassette; aging-dependent sterility; chromatin silencing at HML and HMR; silent mating-type cassette chromatin silencing

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term chromatin silencing at silent mating-type cassette (38 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	chromatin silencing at silent mating-type cassette	38
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term chromatin silencing at silent mating-type cassette or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

chromatin silencing at silent mating-type cassette      38 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
ABF1/YKL112W	IMP: Inferred from Mutant Phenotype Assigned on 2005-10-20	manually curated	Loo S, et al.  (1995) Roles of ABF1, NPL3, and YCL54 in silencing in Saccharomyces cerevisiae. Genetics 141(3):889-902	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2012-02-15	manually curated	Zou Y, et al.  (2006) Asymmetric positioning of nucleosomes and directional establishment of transcriptionally silent chromatin by Saccharomyces cerevisiae silencers. Mol Cell Biol 26(20):7806-19	SGD
ASF1/YJL115W	IGI: Inferred from Genetic Interaction with SGD:RLF2 Assigned on 2006-09-15	manually curated	Mousson F, et al.  (2005) Structural basis for the interaction of Asf1 with histone H3 and its functional implications. Proc Natl Acad Sci U S A 102(17):5975-80	SGD
ASF2/YDL197C	IGI: Inferred from Genetic Interaction Assigned on 2002-11-04	manually curated	Le S, et al.  (1997) Two new S-phase-specific genes from Saccharomyces cerevisiae. Yeast 13(11):1029-42	SGD
ESC2/YDR363W	IMP: Inferred from Mutant Phenotype Assigned on 2002-09-30	manually curated	Dhillon N and Kamakaka RT  (2000) A histone variant, Htz1p, and a Sir1p-like protein, Esc2p, mediate silencing at HMR. Mol Cell 6(4):769-80	SGD
FUN30/YAL019W	IMP: Inferred from Mutant Phenotype Assigned on 2009-12-15	manually curated	Neves-Costa A, et al.  (2009) The SNF2-family member Fun30 promotes gene silencing in heterochromatic loci. PLoS One 4(12):e8111	SGD
HTZ1/YOL012C	IGI: Inferred from Genetic Interaction, IPI: Inferred from Physical Interaction Assigned on 2001-01-18	manually curated	Dhillon N and Kamakaka RT  (2000) A histone variant, Htz1p, and a Sir1p-like protein, Esc2p, mediate silencing at HMR. Mol Cell 6(4):769-80	SGD
MCM10/YIL150C	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction with SGD:SIR2, SGD:SIR3 Assigned on 2005-09-26	manually curated	Liachko I and Tye BK  (2005) Mcm10 is required for the maintenance of transcriptional silencing in Saccharomyces cerevisiae. Genetics 171(2):503-15	SGD
	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction with SGD:SIR2, SGD:SIR3 Assigned on 2006-01-06	manually curated	Douglas NL, et al.  (2005) Dual roles for Mcm10 in DNA replication initiation and silencing at the mating-type loci. Mol Biol Rep 32(4):197-204	SGD
MCM3/YEL032W	IMP: Inferred from Mutant Phenotype Assigned on 2009-01-09	manually curated	Liachko I and Tye BK  (2009) Mcm10 mediates the interaction between DNA replication and silencing machineries. Genetics 181(2):379-91	SGD
MCM7/YBR202W	IMP: Inferred from Mutant Phenotype Assigned on 2009-01-09	manually curated	Liachko I and Tye BK  (2009) Mcm10 mediates the interaction between DNA replication and silencing machineries. Genetics 181(2):379-91	SGD
MGA2/YIR033W	IGI: Inferred from Genetic Interaction with SGD:SIR1, SGD:SUM1 Assigned on 2011-12-14	manually curated	Dula ML and Holmes SG  (2000) MGA2 and SPT23 are modifiers of transcriptional silencing in yeast. Genetics 156(3):933-41	SGD
MRC1/YCL061C	IGI: Inferred from Genetic Interaction, IMP: Inferred from Mutant Phenotype Assigned on 2001-08-14	manually curated	Hu F, et al.  (2001) Asf1 links Rad53 to control of chromatin assembly. Genes Dev 15(9):1061-6	SGD
MSH2/YOL090W	IGI: Inferred from Genetic Interaction with SGD:POL30 Assigned on 2006-09-14	manually curated	Huang S, et al.  (2005) Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing. Proc Natl Acad Sci U S A 102(38):13410-5	SGD
NAM7/YMR080C	IGI: Inferred from Genetic Interaction with SGD:POL30 Assigned on 2006-09-14	manually curated	Huang S, et al.  (2005) Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing. Proc Natl Acad Sci U S A 102(38):13410-5	SGD
NUP133/YKR082W	IDA: Inferred from Direct Assay Assigned on 2012-06-07	manually curated	Ruben GJ, et al.  (2011) Nucleoporin Mediated Nuclear Positioning and Silencing of HMR. PLoS One 6(7):e21923	SGD
NUP2/YLR335W	IMP: Inferred from Mutant Phenotype Assigned on 2012-06-18	manually curated	Ruben GJ, et al.  (2011) Nucleoporin Mediated Nuclear Positioning and Silencing of HMR. PLoS One 6(7):e21923	SGD
NUP60/YAR002W	IMP: Inferred from Mutant Phenotype Assigned on 2012-06-18	manually curated	Ruben GJ, et al.  (2011) Nucleoporin Mediated Nuclear Positioning and Silencing of HMR. PLoS One 6(7):e21923	SGD
NUP84/YDL116W	IDA: Inferred from Direct Assay Assigned on 2012-06-07	manually curated	Ruben GJ, et al.  (2011) Nucleoporin Mediated Nuclear Positioning and Silencing of HMR. PLoS One 6(7):e21923	SGD
ORC1/YML065W	IDA: Inferred from Direct Assay, IGI: Inferred from Genetic Interaction with SGD:SIR1 Assigned on 2007-09-25	manually curated	Connelly JJ, et al.  (2006) Structure and function of the Saccharomyces cerevisiae Sir3 BAH domain. Mol Cell Biol 26(8):3256-65	SGD
	IDA: Inferred from Direct Assay Assigned on 2007-09-26	manually curated	Palacios DeBeer MA, et al.  (2003) Differential DNA affinity specifies roles for the origin recognition complex in budding yeast heterochromatin. Genes Dev 17(15):1817-22	SGD
ORC2/YBR060C	IDA: Inferred from Direct Assay Assigned on 2007-09-26	manually curated	Palacios DeBeer MA, et al.  (2003) Differential DNA affinity specifies roles for the origin recognition complex in budding yeast heterochromatin. Genes Dev 17(15):1817-22	SGD
ORC3/YLL004W	IDA: Inferred from Direct Assay Assigned on 2007-09-26	manually curated	Palacios DeBeer MA, et al.  (2003) Differential DNA affinity specifies roles for the origin recognition complex in budding yeast heterochromatin. Genes Dev 17(15):1817-22	SGD
ORC4/YPR162C	IDA: Inferred from Direct Assay Assigned on 2007-09-26	manually curated	Palacios DeBeer MA, et al.  (2003) Differential DNA affinity specifies roles for the origin recognition complex in budding yeast heterochromatin. Genes Dev 17(15):1817-22	SGD
ORC5/YNL261W	IDA: Inferred from Direct Assay Assigned on 2007-09-26	manually curated	Palacios DeBeer MA, et al.  (2003) Differential DNA affinity specifies roles for the origin recognition complex in budding yeast heterochromatin. Genes Dev 17(15):1817-22	SGD
ORC6/YHR118C	IDA: Inferred from Direct Assay Assigned on 2007-09-26	manually curated	Palacios DeBeer MA, et al.  (2003) Differential DNA affinity specifies roles for the origin recognition complex in budding yeast heterochromatin. Genes Dev 17(15):1817-22	SGD
POL30/YBR088C	IMP: Inferred from Mutant Phenotype Assigned on 2006-09-14	manually curated	Huang S, et al.  (2005) Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing. Proc Natl Acad Sci U S A 102(38):13410-5	SGD
RAP1/YNL216W	IGI: Inferred from Genetic Interaction with SGD:SIR3 Assigned on 2012-05-02	manually curated	Moretti P, et al.  (1994) Evidence that a complex of SIR proteins interacts with the silencer and telomere-binding protein RAP1. Genes Dev 8(19):2257-69	SGD
RIF1/YBR275C	IMP: Inferred from Mutant Phenotype Assigned on 2010-05-12	manually curated	Hardy CF, et al.  (1992) A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation. Genes Dev 6(5):801-14	SGD
SAS3/YBL052C	IMP: Inferred from Mutant Phenotype Assigned on 2002-10-01	manually curated	Osada S, et al.  (2001) The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1. Genes Dev 15(23):3155-68	SGD
SCP160/YJL080C	IMP: Inferred from Mutant Phenotype Assigned on 2006-04-27	manually curated	Marsellach FX, et al.  (2006) The multi-KH domain protein of Saccharomyces cerevisiae Scp160p contributes to the regulation of telomeric silencing. J Biol Chem 281(26):18227-35	SGD
SET1/YHR119W	IMP: Inferred from Mutant Phenotype Assigned on 2005-05-25	manually curated	Nislow C, et al.  (1997) SET1, a yeast member of the trithorax family, functions in transcriptional silencing and diverse cellular processes. Mol Biol Cell 8(12):2421-36	SGD
SIR1/YKR101W	IDA: Inferred from Direct Assay Assigned on 2007-09-26	manually curated	Palacios DeBeer MA, et al.  (2003) Differential DNA affinity specifies roles for the origin recognition complex in budding yeast heterochromatin. Genes Dev 17(15):1817-22	SGD
	IGI: Inferred from Genetic Interaction Assigned on 2002-11-21	manually curated	Rine J, et al.  (1979) A suppressor of mating-type locus mutations in Saccharomyces cerevisiae: evidence for and identification of cryptic mating-type loci. Genetics 93(4):877-901	SGD

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