Ontology: Biological Process (GO:0030433)

Definition: The chemical reactions and pathways resulting in the breakdown of proteins transported from the endoplasmic reticulum and targeted to cytoplasmic proteasomes for degradation. This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins.

Synonyms: endoplasmic reticulum-associated protein catabolic process; endoplasmic reticulum-associated protein catabolism; ER-associated protein breakdown; ER-associated protein catabolism; ER-associated protein degradation; ERAD

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term ER-associated protein catabolic process (42 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	ER-associated protein catabolic process	42
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term ER-associated protein catabolic process or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

ER-associated protein catabolic process      42 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
ADD37/YMR184W	IMP: Inferred from Mutant Phenotype Assigned on 2006-05-11	manually curated	Palmer EA, et al.  (2003) Differential requirements of novel A1PiZ degradation deficient (ADD) genes in ER-associated protein degradation. J Cell Sci 116(Pt 11):2361-73	SGD
ATG19/YOL082W	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-30	manually curated	Mazon MJ, et al.  (2007) Efficient degradation of misfolded mutant Pma1 by endoplasmic reticulum-associated degradation requires Atg19 and the Cvt/autophagy pathway. Mol Microbiol 63(4):1069-1077	SGD
BST1/YFL025C	IMP: Inferred from Mutant Phenotype Assigned on 2002-09-25	manually curated	Vashist S, et al.  (2001) Distinct retrieval and retention mechanisms are required for the quality control of endoplasmic reticulum protein folding. J Cell Biol 155(3):355-68	SGD
CDC48/YDL126C	IMP: Inferred from Mutant Phenotype Assigned on 2003-01-30	manually curated	Rabinovich E, et al.  (2002) AAA-ATPase p97/Cdc48p, a cytosolic chaperone required for endoplasmic reticulum-associated protein degradation. Mol Cell Biol 22(2):626-34	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2012-06-22	manually curated	Bays NW, et al.  (2001) HRD4/NPL4 is required for the proteasomal processing of ubiquitinated ER proteins. Mol Biol Cell 12(12):4114-28	SGD
CNE1/YAL058W	IMP: Inferred from Mutant Phenotype Assigned on 2002-07-17	manually curated	Parlati F, et al.  (1995) Saccharomyces cerevisiae CNE1 encodes an endoplasmic reticulum (ER) membrane protein with sequence similarity to calnexin and calreticulin and functions as a constituent of the ER quality control apparatus. J Biol Chem 270(1):244-53	SGD
CUE1/YMR264W	IMP: Inferred from Mutant Phenotype Assigned on 2002-07-15	manually curated	Biederer T, et al.  (1997) Role of Cue1p in ubiquitination and degradation at the ER surface. Science 278(5344):1806-9	SGD
DER1/YBR201W	IMP: Inferred from Mutant Phenotype Assigned on 2001-12-14	manually curated	Knop M, et al.  (1996) Der1, a novel protein specifically required for endoplasmic reticulum degradation in yeast. EMBO J 15(4):753-63	SGD
	IPI: Inferred from Physical Interaction with SGD:HRD1, SGD:HRD3 Assigned on 2006-12-18	manually curated	Gauss R, et al.  (2006) The Hrd1p ligase complex forms a linchpin between ER-lumenal substrate selection and Cdc48p recruitment. EMBO J 25(9):1827-35	SGD
DFM1/YDR411C	IMP: Inferred from Mutant Phenotype Assigned on 2011-02-17	manually curated	Stolz A, et al.  (2010) Dfm1 forms distinct complexes with Cdc48 and the ER ubiquitin ligases and is required for ERAD. Traffic 11(10):1363-9	SGD
DSK2/YMR276W	IMP: Inferred from Mutant Phenotype Assigned on 2005-03-10	manually curated	Medicherla B, et al.  (2004) A genomic screen identifies Dsk2p and Rad23p as essential components of ER-associated degradation. EMBO Rep 5(7):692-7	SGD
EPS1/YIL005W	IMP: Inferred from Mutant Phenotype Assigned on 2007-11-08	manually curated	Wang Q and Chang A  (2003) Substrate recognition in ER-associated degradation mediated by Eps1, a member of the protein disulfide isomerase family. EMBO J 22(15):3792-802	SGD
HLJ1/YMR161W	IMP: Inferred from Mutant Phenotype Assigned on 2004-11-02	manually curated	Huyer G, et al.  (2004) Distinct machinery is required in Saccharomyces cerevisiae for the endoplasmic reticulum-associated degradation of a multispanning membrane protein and a soluble luminal protein. J Biol Chem 279(37):38369-78	SGD
	IGI: Inferred from Genetic Interaction, IMP: Inferred from Mutant Phenotype Assigned on 2004-11-02	manually curated	Youker RT, et al.  (2004) Distinct roles for the Hsp40 and Hsp90 molecular chaperones during cystic fibrosis transmembrane conductance regulator degradation in yeast. Mol Biol Cell 15(11):4787-97	SGD
HRD1/YOL013C	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction Assigned on 2001-12-12	manually curated	Bays NW, et al.  (2001) Hrd1p/Der3p is a membrane-anchored ubiquitin ligase required for ER-associated degradation. Nat Cell Biol 3(1):24-9	SGD
HRD3/YLR207W	IDA: Inferred from Direct Assay Assigned on 2002-08-01	manually curated	Haynes CM, et al.  (2002) An HRD/DER-independent ER quality control mechanism involves Rsp5p-dependent ubiquitination and ER-Golgi transport. J Cell Biol 158(1):91-101	SGD
HUL5/YGL141W	IMP: Inferred from Mutant Phenotype Assigned on 2008-05-12	manually curated	Crosas B, et al.  (2006) Ubiquitin chains are remodeled at the proteasome by opposing ubiquitin ligase and deubiquitinating activities. Cell 127(7):1401-13	SGD
JEM1/YJL073W	IMP: Inferred from Mutant Phenotype Assigned on 2006-02-01	manually curated	Nishikawa SI, et al.  (2001) Molecular chaperones in the yeast endoplasmic reticulum maintain the solubility of proteins for retrotranslocation and degradation. J Cell Biol 153(5):1061-70	SGD
KAR2/YJL034W	IMP: Inferred from Mutant Phenotype Assigned on 2006-02-01	manually curated	Nishikawa SI, et al.  (2001) Molecular chaperones in the yeast endoplasmic reticulum maintain the solubility of proteins for retrotranslocation and degradation. J Cell Biol 153(5):1061-70	SGD
LCL2/YLR104W	IGI: Inferred from Genetic Interaction Assigned on 2009-10-05	manually curated	Jonikas MC, et al.  (2009) Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum. Science 323(5922):1693-7	SGD
MNL1/YHR204W	IMP: Inferred from Mutant Phenotype Assigned on 2002-08-20	manually curated	Nakatsukasa K, et al.  (2001) Mnl1p, an alpha -mannosidase-like protein in yeast Saccharomyces cerevisiae, is required for endoplasmic reticulum-associated degradation of glycoproteins. J Biol Chem 276(12):8635-8	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2002-08-20	manually curated	Jakob CA, et al.  (2001) Htm1p, a mannosidase-like protein, is involved in glycoprotein degradation in yeast. EMBO Rep 2(5):423-30	SGD
MNL2/YLR057W	IGI: Inferred from Genetic Interaction with SGD:MNL1 Assigned on 2011-10-17	manually curated	Benitez EM, et al.  (2011) Mnl2, a novel component of the ER associated protein degradation pathway. Biochem Biophys Res Commun 414(3):528-32	SGD
MNS1/YJR131W	IMP: Inferred from Mutant Phenotype Assigned on 2005-07-28	manually curated	Knop M, et al.  (1996) N-Glycosylation affects endoplasmic reticulum degradation of a mutated derivative of carboxypeptidase yscY in yeast. Yeast 12(12):1229-38	SGD
NPL4/YBR170C	IMP: Inferred from Mutant Phenotype Assigned on 2003-10-10	manually curated	Bays NW, et al.  (2001) HRD4/NPL4 is required for the proteasomal processing of ubiquitinated ER proteins. Mol Biol Cell 12(12):4114-28	SGD
PBN1/YCL052C	IMP: Inferred from Mutant Phenotype Assigned on 2006-11-02	manually curated	Subramanian S, et al.  (2006) Pbn1p: an essential endoplasmic reticulum membrane protein required for protein processing in the endoplasmic reticulum of budding yeast. Proc Natl Acad Sci U S A 103(4):939-44	SGD
POF1/YCL047C	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction with SGD:UBC7 Assigned on 2012-02-07	manually curated	Costa IM, et al.  (2011) The promoter of filamentation (POF1) protein from Saccharomyces cerevisiae is an ATPase involved in the protein quality control process. BMC Microbiol 11(1):268	SGD
RAD23/YEL037C	IMP: Inferred from Mutant Phenotype Assigned on 2005-03-10	manually curated	Medicherla B, et al.  (2004) A genomic screen identifies Dsk2p and Rad23p as essential components of ER-associated degradation. EMBO Rep 5(7):692-7	SGD
RPT4/YOR259C	IMP: Inferred from Mutant Phenotype Assigned on 2010-09-13	manually curated	Lipson C, et al.  (2008) A proteasomal ATPase contributes to dislocation of endoplasmic reticulum-associated degradation (ERAD) substrates. J Biol Chem 283(11):7166-75	SGD
SCJ1/YMR214W	IMP: Inferred from Mutant Phenotype Assigned on 2006-02-01	manually curated	Nishikawa SI, et al.  (2001) Molecular chaperones in the yeast endoplasmic reticulum maintain the solubility of proteins for retrotranslocation and degradation. J Cell Biol 153(5):1061-70	SGD
SEC13/YLR208W	IMP: Inferred from Mutant Phenotype Assigned on 2012-06-11	manually curated	Fu L and Sztul E  (2003) Traffic-independent function of the Sar1p/COPII machinery in proteasomal sorting of the cystic fibrosis transmembrane conductance regulator. J Cell Biol 160(2):157-63	SGD
SEC61/YLR378C	IPI: Inferred from Physical Interaction with SGD:HRD3, SGD:CDC48 Assigned on 2011-03-09	manually curated	Schafer A and Wolf DH  (2009) Sec61p is part of the endoplasmic reticulum-associated degradation machinery. EMBO J 28(19):2874-84	SGD
SHP1/YBL058W	IMP: Inferred from Mutant Phenotype Assigned on 2011-05-19	manually curated	Tran JR, et al.  (2011) A Cdc48p-associated factor modulates endoplasmic reticulum-associated degradation, cell stress, and ubiquitinated protein homeostasis. J Biol Chem 286(7):5744-55	SGD
SSM4/YIL030C	IMP: Inferred from Mutant Phenotype Assigned on 2005-07-14	manually curated	Swanson R, et al.  (2001) A conserved ubiquitin ligase of the nuclear envelope/endoplasmic reticulum that functions in both ER-associated and Matalpha2 repressor degradation. Genes Dev 15(20):2660-74	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-07-14	manually curated	Huyer G, et al.  (2004) Distinct machinery is required in Saccharomyces cerevisiae for the endoplasmic reticulum-associated degradation of a multispanning membrane protein and a soluble luminal protein. J Biol Chem 279(37):38369-78	SGD

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