Ontology: Biological Process (GO:0030150)

Definition: The import of proteins across the outer and inner mitochondrial membranes into the matrix. Unfolded proteins enter the mitochondrial matrix with a chaperone protein; the information required to target the precursor protein from the cytosol to the mitochondrial matrix is contained within its N-terminal matrix-targeting sequence. Translocation of precursors to the matrix occurs at the rare sites where the outer and inner membranes are close together.

Synonyms: mitochondrial translocation; mitochondrial matrix protein import; protein transport into mitochondrial matrix

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term protein import into mitochondrial matrix (21 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	protein import into mitochondrial matrix	21
High-throughput	none	none
Computational (download data)	protein import into mitochondrial matrix	5

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term protein import into mitochondrial matrix or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

protein import into mitochondrial matrix      21 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
HSP78/YDR258C	IGI: Inferred from Genetic Interaction with SGD:SSC1 Assigned on 2009-01-23	manually curated	Schmitt M, et al.  (1995) Hsp78, a Clp homologue within mitochondria, can substitute for chaperone functions of mt-hsp70. EMBO J 14(14):3434-44	SGD
MDJ2/YNL328C	IGI: Inferred from Genetic Interaction, IPI: Inferred from Physical Interaction Assigned on 2006-03-02	manually curated	Mokranjac D, et al.  (2005) The import motor of the yeast mitochondrial TIM23 preprotein translocase contains two different J proteins, Tim14 and Mdj2. J Biol Chem 280(36):31608-14	SGD
MGE1/YOR232W	IMP: Inferred from Mutant Phenotype Assigned on 2003-11-19	manually curated	Laloraya S, et al.  (1994) A role for a eukaryotic GrpE-related protein, Mge1p, in protein translocation. Proc Natl Acad Sci U S A 91(14):6481-5	SGD
MGR2/YPL098C	IMP: Inferred from Mutant Phenotype Assigned on 2012-06-07	manually curated	Gebert M, et al.  (2012) Mgr2 promotes coupling of the mitochondrial presequence translocase to partner complexes. J Cell Biol 197(5):595-604	SGD
PAM16/YJL104W	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction Assigned on 2004-07-13	manually curated	Frazier AE, et al.  (2004) Pam16 has an essential role in the mitochondrial protein import motor. Nat Struct Mol Biol 11(3):226-33	SGD
	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction Assigned on 2004-07-13	manually curated	Kozany C, et al.  (2004) The J domain-related cochaperone Tim16 is a constituent of the mitochondrial TIM23 preprotein translocase. Nat Struct Mol Biol 11(3):234-41	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR005341 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
PAM17/YKR065C	IMP: Inferred from Mutant Phenotype Assigned on 2007-02-01	manually curated	van der Laan M, et al.  (2005) Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor. Mol Cell Biol 25(17):7449-58	SGD
PAM18/YLR008C	IMP: Inferred from Mutant Phenotype Assigned on 2003-11-26	manually curated	Mokranjac D, et al.  (2003) Tim14, a novel key component of the import motor of the TIM23 protein translocase of mitochondria. EMBO J 22(19):4945-56	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2003-12-10	manually curated	Truscott KN, et al.  (2003) A J-protein is an essential subunit of the presequence translocase-associated protein import motor of mitochondria. J Cell Biol 163(4):707-13	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2003-12-10	manually curated	D'Silva PD, et al.  (2003) J protein cochaperone of the mitochondrial inner membrane required for protein import into the mitochondrial matrix. Proc Natl Acad Sci U S A 100(24):13839-44	SGD
SSC1/YJR045C	IDA: Inferred from Direct Assay Assigned on 2003-11-19	manually curated	Voos W, et al.  (1996) Differential requirement for the mitochondrial Hsp70-Tim44 complex in unfolding and translocation of preproteins. EMBO J 15(11):2668-77	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2007-09-18	manually curated	Voisine C, et al.  (2000) Role of the mitochondrial Hsp70s, Ssc1 and Ssq1, in the maturation of Yfh1. Mol Cell Biol 20(10):3677-84	SGD
TIM17/YJL143W	IMP: Inferred from Mutant Phenotype Assigned on 2009-08-05	manually curated	Berthold J, et al.  (1995) The MIM complex mediates preprotein translocation across the mitochondrial inner membrane and couples it to the mt-Hsp70/ATP driving system. Cell 81(7):1085-93	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-08-05	manually curated	Ryan KR, et al.  (1994) SMS1, a high-copy suppressor of the yeast mas6 mutant, encodes an essential inner membrane protein required for mitochondrial protein import. Mol Biol Cell 5(5):529-38	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2003-12-01	manually curated	Milisav I, et al.  (2001) Modular structure of the TIM23 preprotein translocase of mitochondria. J Biol Chem 276(28):25856-61	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-08-05	manually curated	Meier S, et al.  (2005) Conserved N-terminal negative charges in the Tim17 subunit of the TIM23 translocase play a critical role in the import of preproteins into mitochondria. J Biol Chem 280(9):7777-85	SGD
TIM21/YGR033C	IMP: Inferred from Mutant Phenotype Assigned on 2009-08-05	manually curated	Chacinska A, et al.  (2005) Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell 120(6):817-29	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR013261 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
TIM23/YNR017W	IMP: Inferred from Mutant Phenotype Assigned on 2009-08-05	manually curated	Berthold J, et al.  (1995) The MIM complex mediates preprotein translocation across the mitochondrial inner membrane and couples it to the mt-Hsp70/ATP driving system. Cell 81(7):1085-93	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-08-05	manually curated	Donzeau M, et al.  (2000) Tim23 links the inner and outer mitochondrial membranes. Cell 101(4):401-12	SGD
TIM44/YIL022W	IMP: Inferred from Mutant Phenotype Assigned on 2009-08-05	manually curated	Hutu DP, et al.  (2008) Mitochondrial protein import motor: differential role of tim44 in the recruitment of pam17 and j-complex to the presequence translocase. Mol Biol Cell 19(6):2642-9	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-08-05	manually curated	Maarse AC, et al.  (1992) MPI1, an essential gene encoding a mitochondrial membrane protein, is possibly involved in protein import into yeast mitochondria. EMBO J 11(10):3619-28	SGD
TIM50/YPL063W	IMP: Inferred from Mutant Phenotype Assigned on 2009-08-05	manually curated	Geissler A, et al.  (2002) The mitochondrial presequence translocase: an essential role of Tim50 in directing preproteins to the import channel. Cell 111(4):507-18	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-08-05	manually curated	Yamamoto H, et al.  (2002) Tim50 is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes. Cell 111(4):519-28	SGD
TOM20/YGR082W	IMP: Inferred from Mutant Phenotype Assigned on 2009-04-09	manually curated	Moczko M, et al.  (1994) Deletion of the receptor MOM19 strongly impairs import of cleavable preproteins into Saccharomyces cerevisiae mitochondria. J Biol Chem 269(12):9045-51	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-04-09	manually curated	Ramage L, et al.  (1993) Functional cooperation of mitochondrial protein import receptors in yeast. EMBO J 12(11):4115-23	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2003-12-02	manually curated	Dekker PJ, et al.  (1998) Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex. Mol Cell Biol 18(11):6515-24	SGD
TOM22/YNL131W	IMP: Inferred from Mutant Phenotype Assigned on 2009-04-09	manually curated	van Wilpe S, et al.  (1999) Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase. Nature 401(6752):485-9	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2003-12-02	manually curated	Dekker PJ, et al.  (1998) Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex. Mol Cell Biol 18(11):6515-24	SGD
TOM40/YMR203W	IMP: Inferred from Mutant Phenotype Assigned on 2009-04-03	manually curated	Habib SJ, et al.  (2005) Assembly of the TOB complex of mitochondria. J Biol Chem 280(8):6434-40	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR005686 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
TOM6/YOR045W	IMP: Inferred from Mutant Phenotype Assigned on 2003-12-02	manually curated	Dekker PJ, et al.  (1998) Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex. Mol Cell Biol 18(11):6515-24	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR020266 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
TOM7/YNL070W	IMP: Inferred from Mutant Phenotype Assigned on 2003-12-02	manually curated	Honlinger A, et al.  (1996) Tom7 modulates the dynamics of the mitochondrial outer membrane translocase and plays a pathway-related role in protein import. EMBO J 15(9):2125-37	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR012621 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
TOM70/YNL121C	IMP: Inferred from Mutant Phenotype Assigned on 2009-08-07	manually curated	Moczko M, et al.  (1994) Deletion of the receptor MOM19 strongly impairs import of cleavable preproteins into Saccharomyces cerevisiae mitochondria. J Biol Chem 269(12):9045-51	SGD
XDJ1/YLR090W	IGI: Inferred from Genetic Interaction Assigned on 2013-03-04	manually curated	Sahi C, et al.  (2013) Sequential Duplications of an Ancient Member of the DnaJ-Family Expanded the Functional Chaperone Network in the Eukaryotic Cytosol. Mol Biol Evol 30(5):985-98	SGD
ZIM17/YNL310C	IMP: Inferred from Mutant Phenotype Assigned on 2005-01-03	manually curated	Burri L, et al.  (2004) Zim17, a novel zinc finger protein essential for protein import into mitochondria. J Biol Chem 279(48):50243-9	SGD

SGD Home About Blog Site Map Help Terms of Use
© Stanford University, Stanford, CA 94305.