Ontology: Biological Process (GO:0000027)

Definition: The aggregation, arrangement and bonding together of constituent RNAs and proteins to form the large ribosomal subunit.

Synonyms: 50S ribosomal subunit assembly; 60S ribosomal subunit assembly

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term ribosomal large subunit assembly (33 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	ribosomal large subunit assembly	33
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term ribosomal large subunit assembly or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

ribosomal large subunit assembly      33 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
BRX1/YOL077C	IPI: Inferred from Physical Interaction Assigned on 2002-08-26	manually curated	Fatica A, et al.  (2002) Ssf1p prevents premature processing of an early pre-60S ribosomal particle. Mol Cell 9(2):341-51	SGD
DRS1/YLL008W	IMP: Inferred from Mutant Phenotype Assigned on 2013-05-01	manually curated	Ripmaster TL, et al.  (1992) A putative ATP-dependent RNA helicase involved in Saccharomyces cerevisiae ribosome assembly. Proc Natl Acad Sci U S A 89(23):11131-5	SGD
IPI1/YHR085W	IMP: Inferred from Mutant Phenotype Assigned on 2005-03-10	manually curated	Galani K, et al.  (2004) Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits. J Biol Chem 279(53):55411-8	SGD
IPI3/YNL182C	IMP: Inferred from Mutant Phenotype Assigned on 2005-03-10	manually curated	Galani K, et al.  (2004) Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits. J Biol Chem 279(53):55411-8	SGD
LSG1/YGL099W	IMP: Inferred from Mutant Phenotype Assigned on 2010-09-14	manually curated	Lo KY, et al.  (2010) Defining the pathway of cytoplasmic maturation of the 60S ribosomal subunit. Mol Cell 39(2):196-208	SGD
MAK11/YKL021C	IGI: Inferred from Genetic Interaction with SGD:RLP24, IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction with SGD:RLP24 Assigned on 2007-03-02	manually curated	Saveanu C, et al.  (2007) The p21-activated protein kinase inhibitor Skb15 and its budding yeast homologue are 60S ribosome assembly factors. Mol Cell Biol 27(8):2897-909	SGD
MAK21/YDR060W	IMP: Inferred from Mutant Phenotype Assigned on 2001-07-03	manually curated	Edskes HK, et al.  (1998) Mak21p of Saccharomyces cerevisiae, a homolog of human CAATT-binding protein, is essential for 60 S ribosomal subunit biogenesis. J Biol Chem 273(44):28912-20	SGD
MDN1/YLR106C	IPI: Inferred from Physical Interaction Assigned on 2003-11-03	manually curated	Bassler J, et al.  (2001) Identification of a 60S preribosomal particle that is closely linked to nuclear export. Mol Cell 8(3):517-29	SGD
	IPI: Inferred from Physical Interaction Assigned on 2003-11-03	manually curated	Nissan TA, et al.  (2002) 60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm. EMBO J 21(20):5539-47	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-03-10	manually curated	Galani K, et al.  (2004) Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits. J Biol Chem 279(53):55411-8	SGD
MRT4/YKL009W	IGI: Inferred from Genetic Interaction with SGD:YVH1, IMP: Inferred from Mutant Phenotype Assigned on 2010-02-09	manually curated	Kemmler S, et al.  (2009) Yvh1 is required for a late maturation step in the 60S biogenesis pathway. J Cell Biol 186(6):863-80	SGD
NIP7/YPL211W	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Venema J and Tollervey D  (1999) Ribosome synthesis in Saccharomyces cerevisiae. Annu Rev Genet 33:261-311	SGD
REX4/YOL080C	IGI: Inferred from Genetic Interaction with SGD:RRP5 Assigned on 2008-05-21	manually curated	Eppens NA, et al.  (2002) Deletions in the S1 domain of Rrp5p cause processing at a novel site in ITS1 of yeast pre-rRNA that depends on Rex4p. Nucleic Acids Res 30(19):4222-31	SGD
RIX1/YHR197W	IMP: Inferred from Mutant Phenotype Assigned on 2005-03-10	manually curated	Galani K, et al.  (2004) Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits. J Biol Chem 279(53):55411-8	SGD
RPF1/YHR088W	IPI: Inferred from Physical Interaction Assigned on 2002-08-26	manually curated	Fatica A, et al.  (2002) Ssf1p prevents premature processing of an early pre-60S ribosomal particle. Mol Cell 9(2):341-51	SGD
RPF2/YKR081C	IPI: Inferred from Physical Interaction Assigned on 2002-08-26	manually curated	Fatica A, et al.  (2002) Ssf1p prevents premature processing of an early pre-60S ribosomal particle. Mol Cell 9(2):341-51	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2003-02-19	manually curated	Wehner KA and Baserga SJ  (2002) The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis. Mol Cell 9(2):329-39	SGD
RPL10/YLR075W	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Dick FA and Trumpower BL  (1998) Heterologous complementation reveals that mutant alleles of QSR1 render 60S ribosomal subunits unstable and translationally inactive. Nucleic Acids Res 26(10):2442-8	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-04-10	manually curated	Petrov AN, et al.  (2008) Yeast ribosomal protein L10 helps coordinate tRNA movement through the large subunit. Nucleic Acids Res 36(19):6187-98	SGD
RPL11A/YPR102C	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Moritz M, et al.  (1990) Depletion of yeast ribosomal proteins L16 or rp59 disrupts ribosome assembly. J Cell Biol 111(6 Pt 1):2261-74	SGD
RPL11B/YGR085C	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Moritz M, et al.  (1990) Depletion of yeast ribosomal proteins L16 or rp59 disrupts ribosome assembly. J Cell Biol 111(6 Pt 1):2261-74	SGD
RPL12A/YEL054C	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Briones E, et al.  (1998) The GTPase center protein L12 is required for correct ribosomal stalk assembly but not for Saccharomyces cerevisiae viability. J Biol Chem 273(48):31956-61	SGD
RPL12B/YDR418W	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Briones E, et al.  (1998) The GTPase center protein L12 is required for correct ribosomal stalk assembly but not for Saccharomyces cerevisiae viability. J Biol Chem 273(48):31956-61	SGD
RPL25/YOL127W	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Kooi EA, et al.  (1994) Mutational analysis of the C-terminal region of Saccharomyces cerevisiae ribosomal protein L25 in vitro and in vivo demonstrates the presence of two distinct functional elements. J Mol Biol 240(3):243-55	SGD
RPL3/YOR063W	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Nam HG and Fried HM  (1986) Effects of progressive depletion of TCM1 or CYH2 mRNA on Saccharomyces cerevisiae ribosomal protein accumulation. Mol Cell Biol 6(5):1535-44	SGD
RPL5/YPL131W	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Deshmukh M, et al.  (1993) Yeast ribosomal protein L1 is required for the stability of newly synthesized 5S rRNA and the assembly of 60S ribosomal subunits. Mol Cell Biol 13(5):2835-45	SGD
RPL6A/YML073C	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Rotenberg MO, et al.  (1988) Depletion of Saccharomyces cerevisiae ribosomal protein L16 causes a decrease in 60S ribosomal subunits and formation of half-mer polyribosomes. Genes Dev 2(2):160-72	SGD
RPL6B/YLR448W	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Rotenberg MO, et al.  (1988) Depletion of Saccharomyces cerevisiae ribosomal protein L16 causes a decrease in 60S ribosomal subunits and formation of half-mer polyribosomes. Genes Dev 2(2):160-72	SGD
RPP0/YLR340W	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Santos C and Ballesta JP  (1994) Ribosomal protein P0, contrary to phosphoproteins P1 and P2, is required for ribosome activity and Saccharomyces cerevisiae viability. J Biol Chem 269(22):15689-96	SGD
RSA1/YPL193W	IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Kressler D, et al.  (1999) Synthetic lethality with conditional dbp6 alleles identifies rsa1p, a nucleoplasmic protein involved in the assembly of 60S ribosomal subunits. Mol Cell Biol 19(12):8633-45	SGD
RSA3/YLR221C	IGI: Inferred from Genetic Interaction, IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction Assigned on 2004-05-26	manually curated	De La Cruz J, et al.  (2004) The putative RNA helicase Dbp6p functionally interacts with Rpl3p, Nop8p and the novel trans-acting Factor Rsa3p during biogenesis of 60S ribosomal subunits in Saccharomyces cerevisiae. Genetics 166(4):1687-99	SGD
RSA4/YCR072C	IMP: Inferred from Mutant Phenotype Assigned on 2005-11-08	manually curated	de la Cruz J, et al.  (2005) The essential WD-repeat protein Rsa4p is required for rRNA processing and intra-nuclear transport of 60S ribosomal subunits. Nucleic Acids Res 33(18):5728-39	SGD
SPB4/YFL002C	IMP: Inferred from Mutant Phenotype Assigned on 2011-08-25	manually curated	Garcia-Gomez JJ, et al.  (2011) Dynamics of the putative RNA helicase Spb4 during ribosome assembly in Saccharomyces cerevisiae. Mol Cell Biol 31(20):4156-64	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2013-03-14	manually curated	Sachs AB and Davis RW  (1990) Translation initiation and ribosomal biogenesis: involvement of a putative rRNA helicase and RPL46. Science 247(4946):1077-9	SGD
SQT1/YIR012W	IGI: Inferred from Genetic Interaction, IMP: Inferred from Mutant Phenotype Assigned on 2001-01-18	manually curated	Eisinger DP, et al.  (1997) SQT1, which encodes an essential WD domain protein of Saccharomyces cerevisiae, suppresses dominant-negative mutations of the ribosomal protein gene QSR1. Mol Cell Biol 17(9):5146-55	SGD

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