Ontology: Cellular Component (GO:0000243)

Definition: A spliceosomal complex that is formed by association of the U1 snRNP with the 5' splice site of an unspliced intron in an RNA transcript.

Synonyms: mammalian spliceosomal complex E; mammalian spliceosomal E complex; yeast spliceosomal complex CC

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term commitment complex (12 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	commitment complex	12
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term commitment complex or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

commitment complex      12 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
CBC2/YPL178W	IDA: Inferred from Direct Assay Assigned on 2002-07-30	manually curated	Colot HV, et al.  (1996) The yeast splicing factor Mud13p is a commitment complex component and corresponds to CBP20, the small subunit of the nuclear cap-binding complex. Genes Dev 10(13):1699-708	SGD
MSL5/YLR116W	IDA: Inferred from Direct Assay Assigned on 2005-11-03	manually curated	Abovich N and Rosbash M  (1997) Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals. Cell 89(3):403-12	SGD
	IDA: Inferred from Direct Assay Assigned on 2002-11-18	manually curated	Rutz B and Seraphin B  (1999) Transient interaction of BBP/ScSF1 and Mud2 with the splicing machinery affects the kinetics of spliceosome assembly. RNA 5(6):819-31	SGD
MUD2/YKL074C	IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2009-10-21	manually curated	Abovich N, et al.  (1994) The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition. Genes Dev 8(7):843-54	SGD
NAM8/YHR086W	IPI: Inferred from Physical Interaction Assigned on 2002-04-05	manually curated	Zhang D and Rosbash M  (1999) Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. Genes Dev 13(5):581-92	SGD
PRP39/YML046W	IGI: Inferred from Genetic Interaction with SGD:LSR1 Assigned on 2005-11-02	manually curated	Lockhart SR and Rymond BC  (1994) Commitment of yeast pre-mRNA to the splicing pathway requires a novel U1 small nuclear ribonucleoprotein polypeptide, Prp39p. Mol Cell Biol 14(6):3623-33	SGD
SMD1/YGR074W	IPI: Inferred from Physical Interaction Assigned on 2002-07-29	manually curated	Zhang D and Rosbash M  (1999) Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. Genes Dev 13(5):581-92	SGD
SMD3/YLR147C	IPI: Inferred from Physical Interaction Assigned on 2002-07-29	manually curated	Zhang D and Rosbash M  (1999) Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. Genes Dev 13(5):581-92	SGD
SNP1/YIL061C	IPI: Inferred from Physical Interaction Assigned on 2002-07-29	manually curated	Zhang D and Rosbash M  (1999) Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. Genes Dev 13(5):581-92	SGD
SNR19/snR19	IDA: Inferred from Direct Assay Assigned on 2005-10-31	manually curated	Seraphin B and Rosbash M  (1989) Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing. Cell 59(2):349-58	SGD
SNU56/YDR240C	IPI: Inferred from Physical Interaction Assigned on 2002-04-05	manually curated	Zhang D and Rosbash M  (1999) Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. Genes Dev 13(5):581-92	SGD
STO1/YMR125W	IPI: Inferred from Physical Interaction Assigned on 2002-07-29	manually curated	Zhang D and Rosbash M  (1999) Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. Genes Dev 13(5):581-92	SGD
YHC1/YLR298C	IPI: Inferred from Physical Interaction Assigned on 2002-07-29	manually curated	Zhang D and Rosbash M  (1999) Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. Genes Dev 13(5):581-92	SGD

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