Ontology: Cellular Component (GO:0022626)

Definition: A ribosome located in the cytosol.

Synonyms: 80S ribosome; 70S ribosome

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term cytosolic ribosome (13 genes) or to its variants containing one or more qualifiers (5 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	colocalizes_with: cytosolic ribosome	5
	cytosolic ribosome	13
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term cytosolic ribosome or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

colocalizes_with: cytosolic ribosome      5 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
ARD1/YHR013C	IDA: Inferred from Direct Assay Assigned on 2004-12-01	manually curated	Gautschi M, et al.  (2003) The yeast N(alpha)-acetyltransferase NatA is quantitatively anchored to the ribosome and interacts with nascent polypeptides. Mol Cell Biol 23(20):7403-14	SGD
BUD27/YFL023W	IDA: Inferred from Direct Assay Assigned on 2010-07-08	manually curated	Deplazes A, et al.  (2009) Yeast Uri1p promotes translation initiation and may provide a link to cotranslational quality control. EMBO J 28(10):1429-41	SGD
NAT1/YDL040C	IDA: Inferred from Direct Assay Assigned on 2004-12-01	manually curated	Gautschi M, et al.  (2003) The yeast N(alpha)-acetyltransferase NatA is quantitatively anchored to the ribosome and interacts with nascent polypeptides. Mol Cell Biol 23(20):7403-14	SGD
NAT5/YOR253W	IDA: Inferred from Direct Assay Assigned on 2004-12-01	manually curated	Gautschi M, et al.  (2003) The yeast N(alpha)-acetyltransferase NatA is quantitatively anchored to the ribosome and interacts with nascent polypeptides. Mol Cell Biol 23(20):7403-14	SGD
SNL1/YIL016W	IDA: Inferred from Direct Assay Assigned on 2012-06-14	manually curated	Verghese J and Morano KA  (2012) A lysine-rich region within fungal BAG domain-containing proteins mediates a novel association with ribosomes. Eukaryot Cell 11(8):1003-11	SGD

cytosolic ribosome      13 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
ANB1/YJR047C	ISA: Inferred from Sequence Alignment with SGD:HYP2 Assigned on 2009-05-15	manually curated	Jao DL and Chen KY  (2006) Tandem affinity purification revealed the hypusine-dependent binding of eukaryotic initiation factor 5A to the translating 80S ribosomal complex. J Cell Biochem 97(3):583-98	SGD
FES1/YBR101C	IPI: Inferred from Physical Interaction Assigned on 2003-03-21	manually curated	Kabani M, et al.  (2002) Nucleotide exchange factor for the yeast Hsp70 molecular chaperone Ssa1p. Mol Cell Biol 22(13):4677-89	SGD
GCN1/YGL195W	IDA: Inferred from Direct Assay Assigned on 2002-08-02	manually curated	Marton MJ, et al.  (1997) Evidence that GCN1 and GCN20, translational regulators of GCN4, function on elongating ribosomes in activation of eIF2alpha kinase GCN2. Mol Cell Biol 17(8):4474-89	SGD
GCN2/YDR283C	IDA: Inferred from Direct Assay Assigned on 2002-08-02	manually curated	Ramirez M, et al.  (1991) Ribosome association of GCN2 protein kinase, a translational activator of the GCN4 gene of Saccharomyces cerevisiae. Mol Cell Biol 11(6):3027-36	SGD
GCN20/YFR009W	IDA: Inferred from Direct Assay Assigned on 2002-07-11	manually curated	Marton MJ, et al.  (1997) Evidence that GCN1 and GCN20, translational regulators of GCN4, function on elongating ribosomes in activation of eIF2alpha kinase GCN2. Mol Cell Biol 17(8):4474-89	SGD
HEF3/YNL014W	IPI: Inferred from Physical Interaction Assigned on 2002-10-09	manually curated	Sarthy AV, et al.  (1998) Identification and kinetic analysis of a functional homolog of elongation factor 3, YEF3 in Saccharomyces cerevisiae. Yeast 14(3):239-53	SGD
HYP2/YEL034W	IPI: Inferred from Physical Interaction Assigned on 2009-05-15	manually curated	Jao DL and Chen KY  (2006) Tandem affinity purification revealed the hypusine-dependent binding of eukaryotic initiation factor 5A to the translating 80S ribosomal complex. J Cell Biochem 97(3):583-98	SGD
MAP1/YLR244C	IDA: Inferred from Direct Assay Assigned on 2002-11-14	manually curated	Vetro JA and Chang YH  (2002) Yeast methionine aminopeptidase type 1 is ribosome-associated and requires its N-terminal zinc finger domain for normal function in vivo. J Cell Biochem 85(4):678-88	SGD
RLI1/YDR091C	IDA: Inferred from Direct Assay Assigned on 2005-02-17	manually curated	Dong J, et al.  (2004) The essential ATP-binding cassette protein RLI1 functions in translation by promoting preinitiation complex assembly. J Biol Chem 279(40):42157-68	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-04-04	manually curated	Kispal G, et al.  (2005) Biogenesis of cytosolic ribosomes requires the essential iron-sulphur protein Rli1p and mitochondria. EMBO J 24(3):589-98	SGD
SQT1/YIR012W	IDA: Inferred from Direct Assay Assigned on 2001-01-18	manually curated	Eisinger DP, et al.  (1997) SQT1, which encodes an essential WD domain protein of Saccharomyces cerevisiae, suppresses dominant-negative mutations of the ribosomal protein gene QSR1. Mol Cell Biol 17(9):5146-55	SGD
STM1/YLR150W	IDA: Inferred from Direct Assay Assigned on 2004-08-05	manually curated	Van Dyke MW, et al.  (2004) Stm1p, a G4 quadruplex and purine motif triplex nucleic acid-binding protein, interacts with ribosomes and subtelomeric Y' DNA in Saccharomyces cerevisiae. J Biol Chem 279(23):24323-33	SGD
YEF3/YLR249W	IDA: Inferred from Direct Assay Assigned on 2009-08-31	manually curated	Van Dyke N, et al.  (2009) Stm1p alters the ribosome association of eukaryotic elongation factor 3 and affects translation elongation. Nucleic Acids Res 37(18):6116-25	SGD
YGR054W	IDA: Inferred from Direct Assay Assigned on 2002-10-14	manually curated	Zoll WL, et al.  (2002) Characterization of mammalian eIF2A and identification of the yeast homolog. J Biol Chem 277(40):37079-87	SGD

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