Ontology: Biological Process (GO:0002143)

Definition: The process in which a uridine residue at position 34 in the anticodon of a tRNA is post-transcriptionally thiolated at the C2 position. This process involves transfer of a sulfur from cysteine to position C2 by several steps.

Synonyms: wobble position s2U biosynthesis; tRNA wobble uridine thiolation

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term tRNA wobble position uridine thiolation (5 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	tRNA wobble position uridine thiolation	5
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term tRNA wobble position uridine thiolation or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

tRNA wobble position uridine thiolation      5 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
NCS2/YNL119W	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-17	manually curated	Esberg A, et al.  (2006) Elevated levels of two tRNA species bypass the requirement for elongator complex in transcription and exocytosis. Mol Cell 24(1):139-48	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-16	manually curated	Nakai Y, et al.  (2008) Thio-modification of Yeast Cytosolic tRNA Requires a Ubiquitin-related System That Resembles Bacterial Sulfur Transfer Systems. J Biol Chem 283(41):27469-76	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-17	manually curated	Huang B, et al.  (2008) A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. RNA 14(10):2183-94	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-17	manually curated	Noma A, et al.  (2009) Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions. Nucleic Acids Res 37(4):1335-52	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-11-16	manually curated	Leidel S, et al.  (2009) Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA. Nature 458(7235):228-32	SGD
NCS6/YGL211W	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-16	manually curated	Nakai Y, et al.  (2008) Thio-modification of Yeast Cytosolic tRNA Requires a Ubiquitin-related System That Resembles Bacterial Sulfur Transfer Systems. J Biol Chem 283(41):27469-76	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-17	manually curated	Noma A, et al.  (2009) Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions. Nucleic Acids Res 37(4):1335-52	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-11-16	manually curated	Leidel S, et al.  (2009) Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA. Nature 458(7235):228-32	SGD
TUM1/YOR251C	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-17	manually curated	Noma A, et al.  (2009) Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions. Nucleic Acids Res 37(4):1335-52	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-11-16	manually curated	Leidel S, et al.  (2009) Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA. Nature 458(7235):228-32	SGD
UBA4/YHR111W	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-16	manually curated	Nakai Y, et al.  (2008) Thio-modification of Yeast Cytosolic tRNA Requires a Ubiquitin-related System That Resembles Bacterial Sulfur Transfer Systems. J Biol Chem 283(41):27469-76	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-17	manually curated	Noma A, et al.  (2009) Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions. Nucleic Acids Res 37(4):1335-52	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-11-16	manually curated	Leidel S, et al.  (2009) Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA. Nature 458(7235):228-32	SGD
URM1/YIL008W	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-16	manually curated	Nakai Y, et al.  (2008) Thio-modification of Yeast Cytosolic tRNA Requires a Ubiquitin-related System That Resembles Bacterial Sulfur Transfer Systems. J Biol Chem 283(41):27469-76	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-09-17	manually curated	Noma A, et al.  (2009) Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions. Nucleic Acids Res 37(4):1335-52	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2010-11-16	manually curated	Leidel S, et al.  (2009) Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA. Nature 458(7235):228-32	SGD

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