Ontology: Biological Process (GO:0000209)

Definition: Addition of multiple ubiquitin groups to a protein, forming a ubiquitin chain.

Synonyms: polyubiquitin; protein polyubiquitinylation; protein polyubiquitylation

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term protein polyubiquitination (22 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	protein polyubiquitination	22
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term protein polyubiquitination or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

protein polyubiquitination      22 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
BUL1/YMR275C	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction Assigned on 2001-01-18	manually curated	Yashiroda H, et al.  (1996) Bul1, a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae. Mol Cell Biol 16(7):3255-63	SGD
BUL2/YML111W	IMP: Inferred from Mutant Phenotype, ISS: Inferred from Sequence or structural Similarity Assigned on 2001-01-18	manually curated	Yashiroda H, et al.  (1998) The PY-motif of Bul1 protein is essential for growth of Saccharomyces cerevisiae under various stress conditions. Gene 225(1-2):39-46	SGD
CDC34/YDR054C	IDA: Inferred from Direct Assay Assigned on 2005-10-28	manually curated	Haas AL, et al.  (1991) Ubiquitin conjugation by the yeast RAD6 and CDC34 gene products. Comparison to their putative rabbit homologs, E2(20K) AND E2(32K). J Biol Chem 266(8):5104-12	SGD
GPB1/YOR371C	IDA: Inferred from Direct Assay Assigned on 2010-10-18	manually curated	Phan VT, et al.  (2010) The RasGAP proteins Ira2 and neurofibromin are negatively regulated by Gpb1 in yeast and ETEA in humans. Mol Cell Biol 30(9):2264-79	SGD
HUL5/YGL141W	IDA: Inferred from Direct Assay Assigned on 2008-05-12	manually curated	Crosas B, et al.  (2006) Ubiquitin chains are remodeled at the proteasome by opposing ubiquitin ligase and deubiquitinating activities. Cell 127(7):1401-13	SGD
MET30/YIL046W	IMP: Inferred from Mutant Phenotype Assigned on 2011-03-18	manually curated	Kaiser P, et al.  (1998) Cdc34 and the F-box protein Met30 are required for degradation of the Cdk-inhibitory kinase Swe1. Genes Dev 12(16):2587-97	SGD
MMS2/YGL087C	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction Assigned on 2005-10-26	manually curated	Hofmann RM and Pickart CM  (1999) Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair. Cell 96(5):645-53	SGD
MOT2/YER068W	IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2009-04-21	manually curated	Mersman DP, et al.  (2009) Polyubiquitination of the demethylase Jhd2 controls histone methylation and gene expression. Genes Dev 23(8):951-62	SGD
PEX4/YGR133W	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Hochstrasser M  (1996) Ubiquitin-dependent protein degradation. Annu Rev Genet 30:405-39	SGD
RAD5/YLR032W	IDA: Inferred from Direct Assay Assigned on 2009-09-28	manually curated	Carlile CM, et al.  (2009) Synthesis of free and proliferating cell nuclear antigen-bound polyubiquitin chains by the RING E3 ubiquitin ligase Rad5. J Biol Chem 284(43):29326-34	SGD
RAD6/YGL058W	IMP: Inferred from Mutant Phenotype Assigned on 2009-11-11	manually curated	Watkins JF, et al.  (1993) The extremely conserved amino terminus of RAD6 ubiquitin-conjugating enzyme is essential for amino-end rule-dependent protein degradation. Genes Dev 7(2):250-61	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-11-11	manually curated	Dohmen RJ, et al.  (1991) The N-end rule is mediated by the UBC2(RAD6) ubiquitin-conjugating enzyme. Proc Natl Acad Sci U S A 88(16):7351-5	SGD
RSP5/YER125W	IDA: Inferred from Direct Assay, IMP: Inferred from Mutant Phenotype Assigned on 2008-10-13	manually curated	Huibregtse JM, et al.  (1997) The large subunit of RNA polymerase II is a substrate of the Rsp5 ubiquitin-protein ligase. Proc Natl Acad Sci U S A 94(8):3656-61	SGD
UBC1/YDR177W	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Hochstrasser M  (1996) Ubiquitin-dependent protein degradation. Annu Rev Genet 30:405-39	SGD
UBC11/YOR339C	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Hochstrasser M  (1996) Ubiquitin-dependent protein degradation. Annu Rev Genet 30:405-39	SGD
UBC13/YDR092W	IDA: Inferred from Direct Assay Assigned on 2005-10-26	manually curated	Hofmann RM and Pickart CM  (1999) Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair. Cell 96(5):645-53	SGD
UBC4/YBR082C	IDA: Inferred from Direct Assay Assigned on 2005-01-03	manually curated	Seufert W and Jentsch S  (1990) Ubiquitin-conjugating enzymes UBC4 and UBC5 mediate selective degradation of short-lived and abnormal proteins. EMBO J 9(2):543-50	SGD
UBC5/YDR059C	IDA: Inferred from Direct Assay Assigned on 2005-01-03	manually curated	Seufert W and Jentsch S  (1990) Ubiquitin-conjugating enzymes UBC4 and UBC5 mediate selective degradation of short-lived and abnormal proteins. EMBO J 9(2):543-50	SGD
UBC6/YER100W	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Hochstrasser M  (1996) Ubiquitin-dependent protein degradation. Annu Rev Genet 30:405-39	SGD
UBC8/YEL012W	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Hochstrasser M  (1996) Ubiquitin-dependent protein degradation. Annu Rev Genet 30:405-39	SGD
UBI4/YLL039C	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Hochstrasser M  (1996) Ubiquitin-dependent protein degradation. Annu Rev Genet 30:405-39	SGD
UBR1/YGR184C	IMP: Inferred from Mutant Phenotype Assigned on 2009-11-11	manually curated	Bartel B, et al.  (1990) The recognition component of the N-end rule pathway. EMBO J 9(10):3179-89	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2009-11-11	manually curated	Xie Y and Varshavsky A  (1999) The E2-E3 interaction in the N-end rule pathway: the RING-H2 finger of E3 is required for the synthesis of multiubiquitin chain. EMBO J 18(23):6832-44	SGD
UFD4/YKL010C	TAS: Traceable Author Statement Assigned on 2001-01-18	manually curated	Hochstrasser M  (1996) Ubiquitin-dependent protein degradation. Annu Rev Genet 30:405-39	SGD

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