Ontology: Cellular Component (GO:0019005)

Definition: A ubiquitin ligase complex in which a cullin from the Cul1 subfamily and a RING domain protein form the catalytic core; substrate specificity is conferred by a Skp1 adaptor and an F-box protein. SCF complexes are involved in targeting proteins for degradation by the proteasome. The best characterized complexes are those from yeast and mammals (with core subunits named Cdc53/Cul1, Rbx1/Hrt1/Roc1).

Synonyms: CDL1 complex; CRL1 complex; Cul1-RING ubiquitin ligase complex; cullin-RING ligase 1; SCF complex; SCF complex substrate recognition subunit; Skp1/Cul1/F-box protein complex

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term SCF ubiquitin ligase complex (19 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	SCF ubiquitin ligase complex	19
High-throughput	none	none
Computational (download data)	SCF ubiquitin ligase complex	1

Links to Additional Annotations:

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• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term SCF ubiquitin ligase complex or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

SCF ubiquitin ligase complex      19 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
CDC34/YDR054C	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:CDC53 Assigned on 2007-03-01	manually curated	Skowyra D, et al.  (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91(2):209-19	SGD
	IMP: Inferred from Mutant Phenotype Assigned on 2005-04-08	manually curated	Jaquenoud M, et al.  (1998) The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex. EMBO J 17(18):5360-73	SGD
CDC4/YFL009W	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:SKP1 Assigned on 2007-03-01	manually curated	Skowyra D, et al.  (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91(2):209-19	SGD
	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:SKP1, SGD:CDC53 Assigned on 2007-02-07	manually curated	Feldman RM, et al.  (1997) A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell 91(2):221-30	SGD
CDC53/YDL132W	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:CDC34, SGD:SKP1 Assigned on 2007-03-01	manually curated	Skowyra D, et al.  (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91(2):209-19	SGD
	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:CDC4 Assigned on 2007-02-07	manually curated	Feldman RM, et al.  (1997) A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell 91(2):221-30	SGD
DAS1/YJL149W	IDA: Inferred from Direct Assay Assigned on 2011-03-18	manually curated	Seol JH, et al.  (2001) Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat Cell Biol 3(4):384-91	SGD
	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:SKP1 Assigned on 2011-03-18	manually curated	Kus BM, et al.  (2004) Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro. Proteins 54(3):455-67	SGD
DIA2/YOR080W	IDA: Inferred from Direct Assay Assigned on 2005-02-18	manually curated	Kus BM, et al.  (2004) Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro. Proteins 54(3):455-67	SGD
	IDA: Inferred from Direct Assay Assigned on 2009-11-23	manually curated	Koepp DM, et al.  (2006) The F-box protein Dia2 regulates DNA replication. Mol Biol Cell 17(4):1540-8	SGD
GRR1/YJR090C	IMP: Inferred from Mutant Phenotype Assigned on 2005-04-08	manually curated	Jaquenoud M, et al.  (1998) The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex. EMBO J 17(18):5360-73	SGD
HRT1/YOL133W	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:CDC4, SGD:CDC53, SGD:CDC34 Assigned on 2007-02-21	manually curated	Seol JH, et al.  (1999) Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Genes Dev 13(12):1614-26	SGD
HRT3/YLR097C	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:SKP1 Assigned on 2011-03-18	manually curated	Kus BM, et al.  (2004) Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro. Proteins 54(3):455-67	SGD
	IPI: Inferred from Physical Interaction with SGD:SKP1 Assigned on 2007-06-05	manually curated	Hwang GW, et al.  (2006) Identification of F-box proteins that are involved in resistance to methylmercury in Saccharomyces cerevisiae. FEBS Lett 580(30):6813-6818	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-03-18	manually curated	Willems AR, et al.  (1999) SCF ubiquitin protein ligases and phosphorylation-dependent proteolysis. Philos Trans R Soc Lond B Biol Sci 354(1389):1533-50	SGD
MDM30/YLR368W	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:SKP1 Assigned on 2011-03-18	manually curated	Kus BM, et al.  (2004) Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro. Proteins 54(3):455-67	SGD
	IPI: Inferred from Physical Interaction with SGD:CDC53 Assigned on 2008-07-03	manually curated	Cohen MM, et al.  (2008) Ubiquitin-Proteasome-dependent Degradation of a Mitofusin, a Critical Regulator of Mitochondrial Fusion. Mol Biol Cell 19(6):2457-64	SGD
MET30/YIL046W	IDA: Inferred from Direct Assay Assigned on 2011-03-18	manually curated	Kaiser P, et al.  (1998) Cdc34 and the F-box protein Met30 are required for degradation of the Cdk-inhibitory kinase Swe1. Genes Dev 12(16):2587-97	SGD
MFB1/YDR219C	ISA: Inferred from Sequence Alignment with SGD:CDC4, SGD:DIA2, SGD:GRR1, SGD:HRT3, SGD:MDM30, SGD:MET30, SGD:SAF1, SGD:SKP2, SGD:UFO1, SGD:YDR131C, SGD:YDR306C, SGD:YLR224W Assigned on 2011-03-18	manually curated	Patton EE, et al.  (1998) Combinatorial control in ubiquitin-dependent proteolysis: don't Skp the F-box hypothesis. Trends Genet 14(6):236-43	SGD
SAF1/YBR280C	IDA: Inferred from Direct Assay Assigned on 2011-03-18	manually curated	Seol JH, et al.  (2001) Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat Cell Biol 3(4):384-91	SGD
	IMP: Inferred from Mutant Phenotype, IPI: Inferred from Physical Interaction with SGD:SKP1, ISS: Inferred from Sequence or structural Similarity with SGD:CDC4 Assigned on 2007-05-31	manually curated	Escusa S, et al.  (2007) Skp1-Cullin-F-box-dependent Degradation of Aah1p Requires Its Interaction with the F-box Protein Saf1p. J Biol Chem 282(28):20097-103	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR027046 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
SKP1/YDR328C	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:CDC53, SGD:CDC4 Assigned on 2007-03-01	manually curated	Skowyra D, et al.  (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91(2):209-19	SGD
	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:CDC4 Assigned on 2007-02-07	manually curated	Feldman RM, et al.  (1997) A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell 91(2):221-30	SGD
SKP2/YNL311C	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:SKP1 Assigned on 2011-03-18	manually curated	Kus BM, et al.  (2004) Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro. Proteins 54(3):455-67	SGD
UFO1/YML088W	IDA: Inferred from Direct Assay Assigned on 2011-03-18	manually curated	Seol JH, et al.  (2001) Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat Cell Biol 3(4):384-91	SGD
	IPI: Inferred from Physical Interaction with SGD:CDC53, SGD:SKP1 Assigned on 2011-03-17	manually curated	Kaplun L, et al.  (2003) DNA damage response-mediated degradation of Ho endonuclease via the ubiquitin system involves its nuclear export. J Biol Chem 278(49):48727-34	SGD
	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:SKP1 Assigned on 2011-03-17	manually curated	Kus BM, et al.  (2004) Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro. Proteins 54(3):455-67	SGD
YDR131C	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:SKP1 Assigned on 2011-03-18	manually curated	Kus BM, et al.  (2004) Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro. Proteins 54(3):455-67	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-03-18	manually curated	Willems AR, et al.  (1999) SCF ubiquitin protein ligases and phosphorylation-dependent proteolysis. Philos Trans R Soc Lond B Biol Sci 354(1389):1533-50	SGD
YDR306C	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:SKP1 Assigned on 2011-03-18	manually curated	Kus BM, et al.  (2004) Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro. Proteins 54(3):455-67	SGD
YLR224W	IDA: Inferred from Direct Assay, IPI: Inferred from Physical Interaction with SGD:SKP1 Assigned on 2011-03-18	manually curated	Kus BM, et al.  (2004) Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro. Proteins 54(3):455-67	SGD
YLR352W	IDA: Inferred from Direct Assay Assigned on 2011-03-18	manually curated	Seol JH, et al.  (2001) Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat Cell Biol 3(4):384-91	SGD

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