GO term: mediator complex

Ontology: Cellular Component (GO:0016592)

Definition: A protein complex that interacts with the carboxy-terminal domain of the largest subunit of RNA polymerase II and plays an active role in transducing the signal from a transcription factor to the transcriptional machinery. The mediator complex is required for activation of transcription of most protein-coding genes, but can also act as a transcriptional corepressor. The Saccharomyces complex contains several identifiable subcomplexes: a head domain comprising Srb2, -4, and -5, Med6, -8, and -11, and Rox3 proteins; a middle domain comprising Med1, -4, and -7, Nut1 and -2, Cse2, Rgr1, Soh1, and Srb7 proteins; a tail consisting of Gal11p, Med2p, Pgd1p, and Sin4p; and a regulatory subcomplex comprising Ssn2, -3, and -8, and Srb8 proteins. Metazoan mediator complexes have similar modular structures and include homologs of yeast Srb and Med proteins.

Synonyms: TRAP complex; CDK8-containing TRAP/mediator complex; L mediator complex; Srb-mediator complex

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Annotation Summary

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This table lists the methods used to annotate genes either directly to the term mediator complex (23 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	mediator complex	6
High-throughput	none	none
Computational (download data)	mediator complex	20

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Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Genes Annotated with this Term

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Annotation details for genes that have been directly annotated to the term mediator complex or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

mediator complex 23 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
CSE2/YNR010W	IEA: Inferred from Electronic Annotation with EBI:IPR011425 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
GAL11/YOL051W	IEA: Inferred from Electronic Annotation with EBI:IPR008626 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
MED1/YPR070W	IEA: Inferred from Electronic Annotation with EBI:IPR019680 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
MED11/YMR112C	IEA: Inferred from Electronic Annotation with EBI:IPR019404 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
MED4/YOR174W	IEA: Inferred from Electronic Annotation with EBI:IPR019258 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
MED6/YHR058C	IEA: Inferred from Electronic Annotation with EBI:IPR016612, EBI:IPR007018 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
MED7/YOL135C	IEA: Inferred from Electronic Annotation with EBI:IPR009244 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
MED8/YBR193C	IEA: Inferred from Electronic Annotation with EBI:IPR019364 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
NUT1/YGL151W	IDA: Inferred from Direct Assay Assigned on 2009-04-20	manually curated	Cai G, et al. (2009) Mediator structural conservation and implications for the regulation mechanism. Structure 17(4):559-67	SGD
NUT1/YGL151W	IEA: Inferred from Electronic Annotation with EBI:IPR014801 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
NUT2/YPR168W	IEA: Inferred from Electronic Annotation with EBI:IPR019145 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
PGD1/YGL025C	IEA: Inferred from Electronic Annotation with EBI:IPR020998 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
RGR1/YLR071C	IEA: Inferred from Electronic Annotation with EBI:IPR013947 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
ROX3/YBL093C	IEA: Inferred from Electronic Annotation with EBI:IPR013942 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
SOH1/YGL127C	IEA: Inferred from Electronic Annotation with EBI:IPR008831 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
SRB2/YHR041C	IEA: Inferred from Electronic Annotation with EBI:IPR016532, EBI:IPR013921 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
SRB4/YER022W	IEA: Inferred from Electronic Annotation with EBI:IPR019313 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
SRB5/YGR104C	IEA: Inferred from Electronic Annotation with EBI:IPR019095 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
SRB6/YBR253W	IEA: Inferred from Electronic Annotation with EBI:IPR009332, EBI:IPR016530 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
SRB8/YCR081W	IDA: Inferred from Direct Assay Assigned on 2011-01-24	manually curated	Borggrefe T, et al. (2002) A complex of the Srb8, -9, -10, and -11 transcriptional regulatory proteins from yeast. J Biol Chem 277(46):44202-7	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-02-07	manually curated	Liu Y, et al. (2001) Yeast nuclear extract contains two major forms of RNA polymerase II mediator complexes. J Biol Chem 276(10):7169-75	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR019035 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
SSN2/YDR443C	IDA: Inferred from Direct Assay Assigned on 2011-01-24	manually curated	Borggrefe T, et al. (2002) A complex of the Srb8, -9, -10, and -11 transcriptional regulatory proteins from yeast. J Biol Chem 277(46):44202-7	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-02-07	manually curated	Liu Y, et al. (2001) Yeast nuclear extract contains two major forms of RNA polymerase II mediator complexes. J Biol Chem 276(10):7169-75	SGD
	IDA: Inferred from Direct Assay Assigned on 2011-01-24	manually curated	Guglielmi B, et al. (2004) A high resolution protein interaction map of the yeast Mediator complex. Nucleic Acids Res 32(18):5379-91	SGD
	IEA: Inferred from Electronic Annotation with EBI:IPR009401, EBI:IPR018174 Last Updated 2013-03-02	computational	DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
SSN3/YPL042C	IDA: Inferred from Direct Assay Assigned on 2011-01-24	manually curated	Borggrefe T, et al. (2002) A complex of the Srb8, -9, -10, and -11 transcriptional regulatory proteins from yeast. J Biol Chem 277(46):44202-7	SGD
SSN3/YPL042C	IDA: Inferred from Direct Assay Assigned on 2011-02-07	manually curated	Liu Y, et al. (2001) Yeast nuclear extract contains two major forms of RNA polymerase II mediator complexes. J Biol Chem 276(10):7169-75	SGD
SSN8/YNL025C	IDA: Inferred from Direct Assay Assigned on 2011-01-24	manually curated	Borggrefe T, et al. (2002) A complex of the Srb8, -9, -10, and -11 transcriptional regulatory proteins from yeast. J Biol Chem 277(46):44202-7	SGD
SSN8/YNL025C	IDA: Inferred from Direct Assay Assigned on 2011-02-07	manually curated	Liu Y, et al. (2001) Yeast nuclear extract contains two major forms of RNA polymerase II mediator complexes. J Biol Chem 276(10):7169-75	SGD
TAF14/YPL129W	IPI: Inferred from Physical Interaction Assigned on 2004-10-07	manually curated	Henry NL, et al. (1994) TFIIF-TAF-RNA polymerase II connection. Genes Dev 8(23):2868-78	SGD

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