Ontology: Cellular Component (GO:0016591)

Definition: Large protein complex composed of the RNA polymerase core complex and a variety of other proteins including transcription factor complexes TFIIA, D, E, F, and H which are required for promoter recognition, and the Mediator subcomplex. Catalyzes the synthesis of eukaryotic pre-mRNA.

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term DNA-directed RNA polymerase II, holoenzyme (4 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	DNA-directed RNA polymerase II, holoenzyme	3
High-throughput	none	none
Computational (download data)	DNA-directed RNA polymerase II, holoenzyme	1

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term DNA-directed RNA polymerase II, holoenzyme or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

DNA-directed RNA polymerase II, holoenzyme      4 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
ABD1/YBR236C	IPI: Inferred from Physical Interaction Assigned on 2003-02-04	manually curated	Schroeder SC, et al.  (2000) Dynamic association of capping enzymes with transcribing RNA polymerase II. Genes Dev 14(19):2435-40	SGD
ASK10/YGR097W	IDA: Inferred from Direct Assay Assigned on 2004-01-20	manually curated	Cohen TJ, et al.  (2003) Ask10p mediates the oxidative stress-induced destruction of the Saccharomyces cerevisiae C-type cyclin Ume3p/Srb11p. Eukaryot Cell 2(5):962-70	SGD
IWR1/YDL115C	IPI: Inferred from Physical Interaction with SGD:RPO21, SGD:DST1, SGD:RPB2, SGD:RPB3, SGD:RPB4, SGD:RPB5, SGD:RPB7 Assigned on 2006-04-10	manually curated	Krogan NJ, et al.  (2006) Global landscape of protein complexes in the yeast Saccharomyces cerevisiae. Nature 440(7084):637-43	SGD
SSN8/YNL025C	IEA: Inferred from Electronic Annotation with EBI:IPR023598 Last Updated 2013-03-02	computational	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro

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