Ontology: Cellular Component (GO:0000131)

Definition: The portion of the budding yeast plasma membrane where a daughter cell will emerge. The yeast marks this spot with bud-site selection proteins before bud emergence occurs. Actin is polarized to this spot just prior to and during bud emergence.

View Ontology: (graph) | (text)

This table lists the methods used to annotate genes either directly to the term incipient cellular bud site (58 genes) or to its variants containing one or more qualifiers (0 genes). Note that some genes may have been annotated by more than one method so the numbers in the table below may not add up to the totals given here.

Annotation Method	GO Term	# Yeast Genes Annotated
Manually curated (download data)	incipient cellular bud site	58
High-throughput	none	none
Computational	none	none

Links to Additional Annotations:

• View annotations in multiple organisms using
• Search for S. cerevisiae genes annotated, by the Manually curated or High-throughput methods, to this term or to any terms that are descended from this term, i.e., child terms representing more specific biology than this term.

Annotation details for genes that have been directly annotated to the term incipient cellular bud site or its variants containing one or more qualifiers (NOT, contributes to, or colocalizes with).

incipient cellular bud site      58 genes directly annotated to this term
Locus	Evidence	Annotation Method	Reference	Assigned By
BEM1/YBR200W	IDA: Inferred from Direct Assay Assigned on 2002-08-07	manually curated	Ayscough KR, et al.  (1997) High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A. J Cell Biol 137(2):399-416	SGD
BEM2/YER155C	IDA: Inferred from Direct Assay Assigned on 2009-11-06	manually curated	Knaus M, et al.  (2007) Phosphorylation of Bem2p and Bem3p may contribute to local activation of Cdc42p at bud emergence. EMBO J 26(21):4501-13	SGD
BEM3/YPL115C	IDA: Inferred from Direct Assay Assigned on 2009-11-06	manually curated	Knaus M, et al.  (2007) Phosphorylation of Bem2p and Bem3p may contribute to local activation of Cdc42p at bud emergence. EMBO J 26(21):4501-13	SGD
BNI1/YNL271C	IDA: Inferred from Direct Assay Assigned on 2003-08-27	manually curated	Ozaki-Kuroda K, et al.  (2001) Dynamic localization and function of Bni1p at the sites of directed growth in Saccharomyces cerevisiae. Mol Cell Biol 21(3):827-39	SGD
BNI4/YNL233W	IDA: Inferred from Direct Assay Assigned on 2004-11-12	manually curated	Sanz M, et al.  (2004) Saccharomyces cerevisiae Bni4p directs the formation of the chitin ring and also participates in the correct assembly of the septum structure. Microbiology 150(Pt 10):3229-41	SGD
	IDA: Inferred from Direct Assay Assigned on 2009-11-04	manually curated	Kozubowski L, et al.  (2005) Role of the septin ring in the asymmetric localization of proteins at the mother-bud neck in Saccharomyces cerevisiae. Mol Biol Cell 16(8):3455-66	SGD
BUD14/YAR014C	IDA: Inferred from Direct Assay Assigned on 2002-10-10	manually curated	Ni L and Snyder M  (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12(7):2147-70	SGD
BUD5/YCR038C	IDA: Inferred from Direct Assay Assigned on 2003-01-08	manually curated	Kang PJ, et al.  (2001) A GDP/GTP exchange factor involved in linking a spatial landmark to cell polarity. Science 292(5520):1376-8	SGD
BUD6/YLR319C	IDA: Inferred from Direct Assay Assigned on 2012-11-14	manually curated	Segal M, et al.  (2000) Bud6 directs sequential microtubule interactions with the bud tip and bud neck during spindle morphogenesis in Saccharomyces cerevisiae. Mol Biol Cell 11(11):3689-702	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-11-14	manually curated	Amberg DC, et al.  (1997) Aip3p/Bud6p, a yeast actin-interacting protein that is involved in morphogenesis and the selection of bipolar budding sites. Mol Biol Cell 8(4):729-53	SGD
BUD8/YLR353W	IDA: Inferred from Direct Assay Assigned on 2002-12-01	manually curated	Harkins HA, et al.  (2001) Bud8p and Bud9p, proteins that may mark the sites for bipolar budding in yeast. Mol Biol Cell 12(8):2497-518	SGD
CBK1/YNL161W	IDA: Inferred from Direct Assay Assigned on 2010-01-05	manually curated	Racki WJ, et al.  (2000) Cbk1p, a protein similar to the human myotonic dystrophy kinase, is essential for normal morphogenesis in Saccharomyces cerevisiae. EMBO J 19(17):4524-32	SGD
CDC24/YAL041W	IDA: Inferred from Direct Assay Assigned on 2002-07-10	manually curated	Shimada Y, et al.  (2000) Nuclear sequestration of the exchange factor Cdc24 by Far1 regulates cell polarity during yeast mating. Nat Cell Biol 2(2):117-24	SGD
CDC42/YLR229C	IDA: Inferred from Direct Assay Assigned on 2012-08-13	manually curated	Richman TJ, et al.  (2002) Saccharomyces cerevisiae Cdc42p localizes to cellular membranes and clusters at sites of polarized growth. Eukaryot Cell 1(3):458-68	SGD
	IDA: Inferred from Direct Assay Assigned on 2012-08-13	manually curated	Ziman M, et al.  (1993) Subcellular localization of Cdc42p, a Saccharomyces cerevisiae GTP-binding protein involved in the control of cell polarity. Mol Biol Cell 4(12):1307-16	SGD
CHS3/YBR023C	IDA: Inferred from Direct Assay Assigned on 2004-10-14	manually curated	Santos B and Snyder M  (1997) Targeting of chitin synthase 3 to polarized growth sites in yeast requires Chs5p and Myo2p. J Cell Biol 136(1):95-110	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-05-31	manually curated	Sanz M, et al.  (2004) Saccharomyces cerevisiae Bni4p directs the formation of the chitin ring and also participates in the correct assembly of the septum structure. Microbiology 150(Pt 10):3229-41	SGD
CMD1/YBR109C	IDA: Inferred from Direct Assay Assigned on 2001-01-18	manually curated	Brockerhoff SE and Davis TN  (1992) Calmodulin concentrates at regions of cell growth in Saccharomyces cerevisiae. J Cell Biol 118(3):619-29	SGD
CRH1/YGR189C	IDA: Inferred from Direct Assay Assigned on 2002-12-01	manually curated	Rodriguez-Pena JM, et al.  (2000) A novel family of cell wall-related proteins regulated differently during the yeast life cycle. Mol Cell Biol 20(9):3245-55	SGD
EXO70/YJL085W	IDA: Inferred from Direct Assay Assigned on 2012-09-07	manually curated	Zhang X, et al.  (2008) Membrane association and functional regulation of Sec3 by phospholipids and Cdc42. J Cell Biol 180(1):145-58	SGD
EXO84/YBR102C	IDA: Inferred from Direct Assay Assigned on 2004-07-30	manually curated	Guo W, et al.  (1999) Exo84p is an exocyst protein essential for secretion. J Biol Chem 274(33):23558-64	SGD
GIC1/YHR061C	IDA: Inferred from Direct Assay Assigned on 2002-09-06	manually curated	Chen GC, et al.  (1997) The Cdc42 GTPase-associated proteins Gic1 and Gic2 are required for polarized cell growth in Saccharomyces cerevisiae. Genes Dev 11(22):2958-71	SGD
GIC2/YDR309C	IDA: Inferred from Direct Assay Assigned on 2002-09-06	manually curated	Brown JL, et al.  (1997) Novel Cdc42-binding proteins Gic1 and Gic2 control cell polarity in yeast. Genes Dev 11(22):2972-82	SGD
GYL1/YMR192W	IDA: Inferred from Direct Assay Assigned on 2005-04-14	manually curated	Chesneau L, et al.  (2004) Gyp5p and Gyl1p are involved in the control of polarized exocytosis in budding yeast. J Cell Sci 117(Pt 20):4757-67	SGD
GYP5/YPL249C	IDA: Inferred from Direct Assay Assigned on 2005-04-14	manually curated	Chesneau L, et al.  (2004) Gyp5p and Gyl1p are involved in the control of polarized exocytosis in budding yeast. J Cell Sci 117(Pt 20):4757-67	SGD
HYM1/YKL189W	IDA: Inferred from Direct Assay Assigned on 2003-10-16	manually curated	Nelson B, et al.  (2003) RAM: a conserved signaling network that regulates Ace2p transcriptional activity and polarized morphogenesis. Mol Biol Cell 14(9):3782-803	SGD
KCC4/YCL024W	IDA: Inferred from Direct Assay Assigned on 2009-11-04	manually curated	Kozubowski L, et al.  (2005) Role of the septin ring in the asymmetric localization of proteins at the mother-bud neck in Saccharomyces cerevisiae. Mol Biol Cell 16(8):3455-66	SGD
KIC1/YHR102W	IDA: Inferred from Direct Assay Assigned on 2003-10-16	manually curated	Nelson B, et al.  (2003) RAM: a conserved signaling network that regulates Ace2p transcriptional activity and polarized morphogenesis. Mol Biol Cell 14(9):3782-803	SGD
MMR1/YLR190W	IDA: Inferred from Direct Assay Assigned on 2004-01-15	manually curated	Shepard KA, et al.  (2003) Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-localized transcripts using DNA microarray analysis. Proc Natl Acad Sci U S A 100(20):11429-34	SGD
MSB3/YNL293W	IDA: Inferred from Direct Assay Assigned on 2001-01-18	manually curated	Bi E, et al.  (2000) Identification of novel, evolutionarily conserved Cdc42p-interacting proteins and of redundant pathways linking Cdc24p and Cdc42p to actin polarization in yeast. Mol Biol Cell 11(2):773-93	SGD
MSB4/YOL112W	IDA: Inferred from Direct Assay Assigned on 2001-01-18	manually curated	Bi E, et al.  (2000) Identification of novel, evolutionarily conserved Cdc42p-interacting proteins and of redundant pathways linking Cdc24p and Cdc42p to actin polarization in yeast. Mol Biol Cell 11(2):773-93	SGD
MYO1/YHR023W	IDA: Inferred from Direct Assay Assigned on 2006-09-18	manually curated	Bi E, et al.  (1998) Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis. J Cell Biol 142(5):1301-12	SGD
MYO2/YOR326W	IDA: Inferred from Direct Assay Assigned on 2006-10-18	manually curated	Lillie SH and Brown SS  (1994) Immunofluorescence localization of the unconventional myosin, Myo2p, and the putative kinesin-related protein, Smy1p, to the same regions of polarized growth in Saccharomyces cerevisiae. J Cell Biol 125(4):825-42	SGD
PCL1/YNL289W	IDA: Inferred from Direct Assay Assigned on 2008-02-29	manually curated	Moffat J and Andrews B  (2004) Late-G1 cyclin-CDK activity is essential for control of cell morphogenesis in budding yeast. Nat Cell Biol 6(1):59-66	SGD

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