This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2007-09-17    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
base pairing with rRNA	IGI: Inferred from Genetic Interaction with SGD:ETS1-2, SGD:ETS1-1 Assigned on 2008-06-25 IMP: Inferred from Mutant Phenotype Assigned on 2008-06-25	Beltrame M and Tollervey D  (1995) Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis. EMBO J 14(17):4350-6	SGD
RNA binding	IDA: Inferred from Direct Assay Assigned on 2007-09-17 IPI: Inferred from Physical Interaction with SGD:RDN37-2, SGD:RDN37-1 Assigned on 2006-10-24	Tollervey D  (1987) A yeast small nuclear RNA is required for normal processing of pre-ribosomal RNA. EMBO J 6(13):4169-75	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IGI: Inferred from Genetic Interaction with SGD:ETS1-2, SGD:ETS1-1 Assigned on 2007-09-16	Beltrame M and Tollervey D  (1995) Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis. EMBO J 14(17):4350-6	SGD
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IGI: Inferred from Genetic Interaction with SGD:ETS1-2, SGD:ETS1-1 Assigned on 2007-09-16	Beltrame M and Tollervey D  (1995) Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis. EMBO J 14(17):4350-6	SGD
endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IGI: Inferred from Genetic Interaction with SGD:ETS1-2, SGD:ETS1-1 Assigned on 2007-09-16	Beltrame M and Tollervey D  (1995) Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis. EMBO J 14(17):4350-6	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
90S preribosome	IDA: Inferred from Direct Assay Assigned on 2007-09-18	Dosil M and Bustelo XR  (2004) Functional characterization of Pwp2, a WD family protein essential for the assembly of the 90 S pre-ribosomal particle. J Biol Chem 279(36):37385-97	SGD
box C/D snoRNP complex	IPI: Inferred from Physical Interaction with SGD:NOP58, SGD:NOP56 Assigned on 2007-05-16	Lafontaine DL and Tollervey D  (2000) Synthesis and assembly of the box C+D small nucleolar RNPs. Mol Cell Biol 20(8):2650-9	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-08-13	Lafontaine DL and Tollervey D  (1999) Nop58p is a common component of the box C+D snoRNPs that is required for snoRNA stability. RNA 5(3):455-67	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-07-11	Watkins NJ, et al.  (2000) A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP. Cell 103(3):457-66	SGD
nucleolus	IDA: Inferred from Direct Assay Assigned on 2006-09-19	Morrissey JP and Tollervey D  (1993) Yeast snR30 is a small nucleolar RNA required for 18S rRNA synthesis. Mol Cell Biol 13(4):2469-77	SGD
	IDA: Inferred from Direct Assay Assigned on 2006-10-24	Tollervey D  (1987) A yeast small nuclear RNA is required for normal processing of pre-ribosomal RNA. EMBO J 6(13):4169-75	SGD
small-subunit processome	IDA: Inferred from Direct Assay Assigned on 2007-08-03	Dragon F, et al.  (2002) A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature 417(6892):967-70	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
90S preribosome	IDA: Inferred from Direct Assay Assigned on 2012-02-08	Grandi P, et al.  (2002) 90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors. Mol Cell 10(1):105-15	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

There are no Computational annotations for SNR17A

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).