This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2002-07-17    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
pre-mRNA 5'-splice site binding	IDA: Inferred from Direct Assay Assigned on 2008-04-02	McGrail JC and O'Keefe RT  (2008) The U1, U2 and U5 snRNAs crosslink to the 5' exon during yeast pre-mRNA splicing. Nucleic Acids Res 36(3):814-25	SGD
	IDA: Inferred from Direct Assay Assigned on 2008-04-08	O'Keefe RT and Newman AJ  (1998) Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing. EMBO J 17(2):565-74	SGD
	IDA: Inferred from Direct Assay Assigned on 2002-07-17	Alvi RK, et al.  (2001) ATP-dependent interaction of yeast U5 snRNA loop 1 with the 5' splice site. RNA 7(7):1013-23	SGD
contributes_to second spliceosomal transesterification activity	IMP: Inferred from Mutant Phenotype Assigned on 2008-04-08	O'Keefe RT and Newman AJ  (1998) Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing. EMBO J 17(2):565-74	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
alignment of 3' and 5' splice sites of mRNA	IMP: Inferred from Mutant Phenotype Assigned on 2008-04-14	O'Keefe RT and Newman AJ  (1998) Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing. EMBO J 17(2):565-74	SGD
generation of catalytic spliceosome for second transesterification step	IMP: Inferred from Mutant Phenotype Assigned on 2008-04-08	O'Keefe RT and Newman AJ  (1998) Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing. EMBO J 17(2):565-74	SGD
mRNA 5'-splice site recognition	IDA: Inferred from Direct Assay Assigned on 2008-04-02	McGrail JC and O'Keefe RT  (2008) The U1, U2 and U5 snRNAs crosslink to the 5' exon during yeast pre-mRNA splicing. Nucleic Acids Res 36(3):814-25	SGD
mRNA splicing, via spliceosome	IMP: Inferred from Mutant Phenotype Assigned on 2002-07-17	Patterson B and Guthrie C  (1987) An essential yeast snRNA with a U5-like domain is required for splicing in vivo. Cell 49(5):613-24	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
U4/U6 x U5 tri-snRNP complex	IDA: Inferred from Direct Assay Assigned on 2005-10-03	Gottschalk A, et al.  (1999) Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP. EMBO J 18(16):4535-48	SGD
	IDA: Inferred from Direct Assay Assigned on 2005-02-19	Stevens SW and Abelson J  (1999) Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins. Proc Natl Acad Sci U S A 96(13):7226-31	SGD
U5 snRNP	IDA: Inferred from Direct Assay Assigned on 2002-07-17	Stevens SW, et al.  (2001) Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae. RNA 7(11):1543-53	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

There are no High-throughput annotations for SNR7-L

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

There are no Computational annotations for SNR7-L

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).