This page displays GO annotations in different sections according to the annotation method used to add that annotation to SGD.

Last Reviewed on: 2003-10-28    Molecular Function | Biological Process | Cellular Component

Manually curated Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
peptide alpha-N-acetyltransferase activity	ISS: Inferred from Sequence or structural Similarity Assigned on 2003-01-28 IMP: Inferred from Mutant Phenotype Assigned on 2003-01-28	Polevoda B, et al.  (1999) Identification and specificities of N-terminal acetyltransferases from Saccharomyces cerevisiae. EMBO J 18(21):6155-68	SGD

Manually curated Biological Process
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoskeleton organization	IGI: Inferred from Genetic Interaction with SGD:MDM20 Assigned on 2005-06-15 IMP: Inferred from Mutant Phenotype Assigned on 2005-06-15	Singer JM and Shaw JM  (2003) Mdm20 protein functions with Nat3 protein to acetylate Tpm1 protein and regulate tropomyosin-actin interactions in budding yeast. Proc Natl Acad Sci U S A 100(13):7644-9	SGD
mitochondrion inheritance	IGI: Inferred from Genetic Interaction with SGD:MDM20 Assigned on 2005-06-15 IMP: Inferred from Mutant Phenotype Assigned on 2005-06-15	Singer JM and Shaw JM  (2003) Mdm20 protein functions with Nat3 protein to acetylate Tpm1 protein and regulate tropomyosin-actin interactions in budding yeast. Proc Natl Acad Sci U S A 100(13):7644-9	SGD
N-terminal peptidyl-methionine acetylation	IMP: Inferred from Mutant Phenotype Assigned on 2002-07-11	Polevoda B, et al.  (1999) Identification and specificities of N-terminal acetyltransferases from Saccharomyces cerevisiae. EMBO J 18(21):6155-68	SGD

Manually curated Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
NatB complex	IDA: Inferred from Direct Assay Assigned on 2010-07-01	Polevoda B, et al.  (2003) Nat3p and Mdm20p are required for function of yeast NatB Nalpha-terminal acetyltransferase and of actin and tropomyosin. J Biol Chem 278(33):30686-97	SGD

* Manually curated GO annotations reflect our best understanding of the basic molecular function, biological process, and cellular component for this gene product. Manually curated annotations are assigned by SGD curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all Manually curated GO annotations for accuracy and completeness. The "Last Reviewed on:" date at the top of this section indicates when these annotations were last reviewed.

Cellular Component

High-throughput Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IDA: Inferred from Direct Assay Assigned on 2003-10-28	Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91	SGD

** GO annotations from High-throughput experiments are made based on a variety of large scale high-throughput experiments, including genome-wide experiments. Many of these annotations are made based on GO annotations (or mappings to GO annotations) assigned by the authors, rather than SGD curators. While SGD curators read these publications and often work closely with authors to incorporate the information, each individual annotation may not necessarily be reviewed by a curator. GO Annotations from high-throughput experiments will be assigned only when this type of data is available, and thus may not be assigned in all three aspects of the Gene Ontologies.

Molecular Function | Cellular Component

Computational Molecular Function
Annotation(s)	Evidence	Reference(s)	Assigned By
N-acetyltransferase activity	IEA: Inferred from Electronic Annotation with EBI:IPR000182 Last updated 2013-03-02	DDB, et al.  (2001) Gene Ontology annotation through association of InterPro records with GO terms.	InterPro
peptide alpha-N-acetyltransferase activity	IEA: Inferred from Electronic Annotation with IUBMB:2.3.1.88 Last updated 2013-03-02	GOA curators and MGI curators  (2001) Gene Ontology annotation based on Enzyme Commission mapping.	UniProtKB
transferase activity	IEA: Inferred from Electronic Annotation with EBI:KW-0808 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB
transferase activity, transferring acyl groups	IEA: Inferred from Electronic Annotation with EBI:KW-0012 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

Computational Cellular Component
Annotation(s)	Evidence	Reference(s)	Assigned By
cytoplasm	IEA: Inferred from Electronic Annotation with EBI:SL-0086 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.	UniProtKB
	IEA: Inferred from Electronic Annotation with EBI:KW-0963 Last updated 2013-03-02	UniProt-GOA  (2011) Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.	UniProtKB

*** Computational GO Annotations are predictions. These annotations are NOT reviewed by a curator. Currently, all computational GO annotations for S. cerevisiae are assigned by an external source (for example, the Gene Ontology Annotation (GOA) project of the European Bioinformatics Institute (EBI)).